Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET-G4-1

Bacterial expression vector of human galectin-4 (tag-free protein).

Sequence (full)	RDB19437zzt01.seq compiled by us

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19437_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, insert 5' Sequence file: RDB19437_B1Kqa.seq
>D08453A3_B1Kq_3_pET-B_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNNNCGGC NNCGATGCGT CCGGCGTANA GGNNCGAGAT    61 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC   121 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGGCC TATGTCCCCG   181 CACCGGGCTA CCAGCCCACC TACAACCCGA CGCTGCCTTA CTACCAGCCC ATCCCGGGCG   241 GGCTCAACGT GGGAATGTCT GTTTACATCC AAGGAGTGGC CAGCGAGCAC ATGAAGCGGT   301 TCTTCGTGAA CTTTGTGGTT GGGCAGGATC CGGGCTCAGA CGTCGCCTTC CACTTCAATC   361 CGCGGTTTGA CGGCTGGGAC AAGGTGGTCT TCAACACGTT GCAGGGCGGG AAGTGGGGCA   421 GCGAGGAGAG GAAGAGGAGC ATGCCCTTCA AAAAGGGTGC CGCCTTTGAG CTGGTCTTCA   481 TAGTCCTGGC TGAGCACTAC AAGGTGGTGG TAAATGGAAA TCCCTTCTAT GAGTACGGGC   541 ACCGGCTTCC CCTACAGATG GTCACCCACC TGCAAGTGGA TGGGGATCTG CAACTTCAAT   601 CAATCAACTT CATCGGAGGC CAGCCCCTCC GGCCCCAGGG ACCCCCGATG ATGCCACCTT   661 ACCCTGGTCC CGGACATTGC CATCAACAGC TGAACAGCCT GCCCACCATG GAAGGACCCC   721 CAACCTTCAA CCCGCCTGTG CCATATTTCG GGAGGCTGCA AGGAGGGCTC ACAGCTCGAA   781 NAACCATCAT CATCAAGGGC TATGTGCCTC CCACAGGCAA GAGCTTTGCT ATCAACTTCN   841 AGGTGGGCTC CTCANGGGAC ATAGCTCTGC ACATTAATCC CCGCANGGGC NACGGTACCG   901 NGGTCCGGAA CAGCCTNCTG ANNGGNTCGN GGGGNTCCGA NNANAAGAAN ANCACCCNCN 961 NCCCATTNNG NCCCNGANNN NNNNTTGATC NNNNCATNCN CTGNNNNTNN NNNNNNNN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert 3' Sequence file: RDB19437_B1Kqb.seq
>D08453A3_B1Kq_3_pET-check-R1_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCGCN GNCNGGCNCC GNGTATGAAA TCTAACAATG    61 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC   121 CGGTACTGCC GGGCCTCTTG CGGGATATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC   181 CCTCAAGACC CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC   241 AGCCAACTCA GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCTTTAGATT TGGACATAGG   301 ACAAGGTGAC ATCACCCTGG ATTTCCAATG TGTCCACCCT CTGGAAGGCC GAGAGGCGAT   361 GGGCAAAGTC AAAGAGGTGC TGGCCATTGG CGTAAACCTT GAAGCGATCC AAGCCACAGC   421 GAATGGACAG ATCAAAGAAC TGTCCGGGAC CAAATGGGTT GTGGGTGATC TTCTTCTCCT   481 CGGATCCCCA CGAGCCATTC AGAAGGCTGT TCCGGACCAC GGTACCGTTG CCCATGCGGG   541 GATTAATGTG CAGAGCTATG TCCCCTGAGG AGCCCACCTT GAAGTTGATA GCAAAGCTCT   601 TGCCTGTGGG AGGCACATAG CCCTTGATGA TGATGGTTCT TCGAGCTGTG AGCCCTCCTT   661 GCAGCCTCCC GAAATATGGC ACAGGCGGGT TGAAGGTTGG GGGTCCTTCC ATGGTGGGCA   721 GGCTGTTCAG CTGTTGATGG CAATGTCCGG GACCAGGGTA AGGTGGCATC ATCGGGGGTC   781 CCTGGGGCCG GANGGGCTGG NCTCCGATGA AGTTGANTGA TTGAAGTTGC ANATCCCCAT   841 CCNCTTGCNN GTGGGTGANC NTCTGTAGGG GAAGCCNGNG CCCGTNCTCN TANAANNGNA   901 NTTCNNTTTA CCACCACCTT GNANNGNTCA NCCAGGANTA TGNNNNCAGN TNNAANNNNG 961 CACCCTTTTT NNANGGNNAT GNNNCNNNTT NNNNNNNNNN //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19437_B1Kqc.seq
>D08453A3_B1Kq_3_lacIpro_F_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNGCNNCNN GANANANTCA TTCNGGGTGG TGAATGTGAA ACCAGTAACG    61 TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC   121 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG   181 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC   241 GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT   301 CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA   361 GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC   421 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG   481 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC   541 GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA   601 GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC   661 ACTCGNAATC AAATTCNNNN NANANCNNNN NGGGNNNNNN ANNNNNNNGN CNNGNCCNNN 721 NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl