Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET-G4-1

Bacterial expression vector of human galectin-4 (tag-free protein).

Catalog number RDB19437
Resource name pET-G4-1
Clone info. Bacterial expression vector of human galectin-4 (tag-free protein).
Comment Expression was confirmed by the depositor with SDS-PAGE and Western blotting.
Vector backbone pET-11a (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Ampicillin
Gene/insert name human LGALS4 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
human LGALS4

          Reference sequence
            
           
          Sequence (full) RDB19437zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.


          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースの利用は、学術機関の学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19437 pET-G4-1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19437_B1Kqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pET-B_F
          Region: T7 pro, lacO, RBS, insert 5'
          Sequence file: RDB19437_B1Kqa.seq check
          >D08453A3_B1Kq_3_pET-B_F_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNNCGGC NNCGATGCGT CCGGCGTANA GGNNCGAGAT
             61 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC
            121 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGGCC TATGTCCCCG
            181 CACCGGGCTA CCAGCCCACC TACAACCCGA CGCTGCCTTA CTACCAGCCC ATCCCGGGCG
            241 GGCTCAACGT GGGAATGTCT GTTTACATCC AAGGAGTGGC CAGCGAGCAC ATGAAGCGGT
            301 TCTTCGTGAA CTTTGTGGTT GGGCAGGATC CGGGCTCAGA CGTCGCCTTC CACTTCAATC
            361 CGCGGTTTGA CGGCTGGGAC AAGGTGGTCT TCAACACGTT GCAGGGCGGG AAGTGGGGCA
            421 GCGAGGAGAG GAAGAGGAGC ATGCCCTTCA AAAAGGGTGC CGCCTTTGAG CTGGTCTTCA
            481 TAGTCCTGGC TGAGCACTAC AAGGTGGTGG TAAATGGAAA TCCCTTCTAT GAGTACGGGC
            541 ACCGGCTTCC CCTACAGATG GTCACCCACC TGCAAGTGGA TGGGGATCTG CAACTTCAAT
            601 CAATCAACTT CATCGGAGGC CAGCCCCTCC GGCCCCAGGG ACCCCCGATG ATGCCACCTT
            661 ACCCTGGTCC CGGACATTGC CATCAACAGC TGAACAGCCT GCCCACCATG GAAGGACCCC
            721 CAACCTTCAA CCCGCCTGTG CCATATTTCG GGAGGCTGCA AGGAGGGCTC ACAGCTCGAA
            781 NAACCATCAT CATCAAGGGC TATGTGCCTC CCACAGGCAA GAGCTTTGCT ATCAACTTCN
            841 AGGTGGGCTC CTCANGGGAC ATAGCTCTGC ACATTAATCC CCGCANGGGC NACGGTACCG
            901 NGGTCCGGAA CAGCCTNCTG ANNGGNTCGN GGGGNTCCGA NNANAAGAAN ANCACCCNCN
          961 NCCCATTNNG NCCCNGANNN NNNNTTGATC NNNNCATNCN CTGNNNNTNN NNNNNNNN
          //
          Primer: pET-check-R1
          Region: T7 ter, insert 3'
          Sequence file: RDB19437_B1Kqb.seq check
          >D08453A3_B1Kq_3_pET-check-R1_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNCGCN GNCNGGCNCC GNGTATGAAA TCTAACAATG
             61 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC
            121 CGGTACTGCC GGGCCTCTTG CGGGATATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC
            181 CCTCAAGACC CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC
            241 AGCCAACTCA GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCTTTAGATT TGGACATAGG
            301 ACAAGGTGAC ATCACCCTGG ATTTCCAATG TGTCCACCCT CTGGAAGGCC GAGAGGCGAT
            361 GGGCAAAGTC AAAGAGGTGC TGGCCATTGG CGTAAACCTT GAAGCGATCC AAGCCACAGC
            421 GAATGGACAG ATCAAAGAAC TGTCCGGGAC CAAATGGGTT GTGGGTGATC TTCTTCTCCT
            481 CGGATCCCCA CGAGCCATTC AGAAGGCTGT TCCGGACCAC GGTACCGTTG CCCATGCGGG
            541 GATTAATGTG CAGAGCTATG TCCCCTGAGG AGCCCACCTT GAAGTTGATA GCAAAGCTCT
            601 TGCCTGTGGG AGGCACATAG CCCTTGATGA TGATGGTTCT TCGAGCTGTG AGCCCTCCTT
            661 GCAGCCTCCC GAAATATGGC ACAGGCGGGT TGAAGGTTGG GGGTCCTTCC ATGGTGGGCA
            721 GGCTGTTCAG CTGTTGATGG CAATGTCCGG GACCAGGGTA AGGTGGCATC ATCGGGGGTC
            781 CCTGGGGCCG GANGGGCTGG NCTCCGATGA AGTTGANTGA TTGAAGTTGC ANATCCCCAT
            841 CCNCTTGCNN GTGGGTGANC NTCTGTAGGG GAAGCCNGNG CCCGTNCTCN TANAANNGNA
            901 NTTCNNTTTA CCACCACCTT GNANNGNTCA NCCAGGANTA TGNNNNCAGN TNNAANNNNG
          961 CACCCTTTTT NNANGGNNAT GNNNCNNNTT NNNNNNNNNN
          //
          Primer: lacIpro_F
          Region: lacI pro, lacI
          Sequence file: RDB19437_B1Kqc.seq check
          >D08453A3_B1Kq_3_lacIpro_F_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNGCNNCNN GANANANTCA TTCNGGGTGG TGAATGTGAA ACCAGTAACG
             61 TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC
            121 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG
            181 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC
            241 GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT
            301 CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA
            361 GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC
            421 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG
            481 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC
            541 GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA
            601 GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC
            661 ACTCGNAATC AAATTCNNNN NANANCNNNN NGGGNNNNNN ANNNNNNNGN CNNGNCCNNN
          721 NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl