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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET-G4-1 (#RDB19437)

Bacterial expression vector of human galectin-4 (tag-free protein).

Clone info. Bacterial expression vector of human galectin-4 (tag-free protein).
Comment Expression was confirmed by the depositor with SDS-PAGE and Western blotting.
Vector backbone pET-11a (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Ampicillin
Gene/insert name human LGALS4 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence (full) RDB19437zzt01.seq compiled by us

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19437 pET-G4-1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-G4-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19437).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19437_B1Kqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723)
Region: T7 pro, lacO, RBS, insert 5'
Sequence file: RDB19437_B1Kqa.seq check
>D08453A3_B1Kq_3_pET-B_F_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNNCGGC NNCGATGCGT CCGGCGTANA GGNNCGAGAT
   61 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC
  121 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGGCC TATGTCCCCG
  181 CACCGGGCTA CCAGCCCACC TACAACCCGA CGCTGCCTTA CTACCAGCCC ATCCCGGGCG
  241 GGCTCAACGT GGGAATGTCT GTTTACATCC AAGGAGTGGC CAGCGAGCAC ATGAAGCGGT
  301 TCTTCGTGAA CTTTGTGGTT GGGCAGGATC CGGGCTCAGA CGTCGCCTTC CACTTCAATC
  361 CGCGGTTTGA CGGCTGGGAC AAGGTGGTCT TCAACACGTT GCAGGGCGGG AAGTGGGGCA
  421 GCGAGGAGAG GAAGAGGAGC ATGCCCTTCA AAAAGGGTGC CGCCTTTGAG CTGGTCTTCA
  481 TAGTCCTGGC TGAGCACTAC AAGGTGGTGG TAAATGGAAA TCCCTTCTAT GAGTACGGGC
  541 ACCGGCTTCC CCTACAGATG GTCACCCACC TGCAAGTGGA TGGGGATCTG CAACTTCAAT
  601 CAATCAACTT CATCGGAGGC CAGCCCCTCC GGCCCCAGGG ACCCCCGATG ATGCCACCTT
  661 ACCCTGGTCC CGGACATTGC CATCAACAGC TGAACAGCCT GCCCACCATG GAAGGACCCC
  721 CAACCTTCAA CCCGCCTGTG CCATATTTCG GGAGGCTGCA AGGAGGGCTC ACAGCTCGAA
  781 NAACCATCAT CATCAAGGGC TATGTGCCTC CCACAGGCAA GAGCTTTGCT ATCAACTTCN
  841 AGGTGGGCTC CTCANGGGAC ATAGCTCTGC ACATTAATCC CCGCANGGGC NACGGTACCG
  901 NGGTCCGGAA CAGCCTNCTG ANNGGNTCGN GGGGNTCCGA NNANAAGAAN ANCACCCNCN
961 NCCCATTNNG NCCCNGANNN NNNNTTGATC NNNNCATNCN CTGNNNNTNN NNNNNNNN
//
Primer: pET-check-R1 (Pr0192)
Region: T7 ter, insert 3'
Sequence file: RDB19437_B1Kqb.seq check
>D08453A3_B1Kq_3_pET-check-R1_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCGCN GNCNGGCNCC GNGTATGAAA TCTAACAATG
   61 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC
  121 CGGTACTGCC GGGCCTCTTG CGGGATATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC
  181 CCTCAAGACC CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC
  241 AGCCAACTCA GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCTTTAGATT TGGACATAGG
  301 ACAAGGTGAC ATCACCCTGG ATTTCCAATG TGTCCACCCT CTGGAAGGCC GAGAGGCGAT
  361 GGGCAAAGTC AAAGAGGTGC TGGCCATTGG CGTAAACCTT GAAGCGATCC AAGCCACAGC
  421 GAATGGACAG ATCAAAGAAC TGTCCGGGAC CAAATGGGTT GTGGGTGATC TTCTTCTCCT
  481 CGGATCCCCA CGAGCCATTC AGAAGGCTGT TCCGGACCAC GGTACCGTTG CCCATGCGGG
  541 GATTAATGTG CAGAGCTATG TCCCCTGAGG AGCCCACCTT GAAGTTGATA GCAAAGCTCT
  601 TGCCTGTGGG AGGCACATAG CCCTTGATGA TGATGGTTCT TCGAGCTGTG AGCCCTCCTT
  661 GCAGCCTCCC GAAATATGGC ACAGGCGGGT TGAAGGTTGG GGGTCCTTCC ATGGTGGGCA
  721 GGCTGTTCAG CTGTTGATGG CAATGTCCGG GACCAGGGTA AGGTGGCATC ATCGGGGGTC
  781 CCTGGGGCCG GANGGGCTGG NCTCCGATGA AGTTGANTGA TTGAAGTTGC ANATCCCCAT
  841 CCNCTTGCNN GTGGGTGANC NTCTGTAGGG GAAGCCNGNG CCCGTNCTCN TANAANNGNA
  901 NTTCNNTTTA CCACCACCTT GNANNGNTCA NCCAGGANTA TGNNNNCAGN TNNAANNNNG
961 CACCCTTTTT NNANGGNNAT GNNNCNNNTT NNNNNNNNNN
//
Primer: lacIpro_F (Pr0409)
Region: lacI pro, lacI
Sequence file: RDB19437_B1Kqc.seq check
>D08453A3_B1Kq_3_lacIpro_F_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNGCNNCNN GANANANTCA TTCNGGGTGG TGAATGTGAA ACCAGTAACG
   61 TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC
  121 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG
  181 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC
  241 GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT
  301 CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA
  361 GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC
  421 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG
  481 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC
  541 GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA
  601 GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG CTGGCTGGCA TAAATATCTC
  661 ACTCGNAATC AAATTCNNNN NANANCNNNN NGGGNNNNNN ANNNNNNNGN CNNGNCCNNN
721 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]