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pGEX-G1-1a (#RDB04217)

Bacteria expression vector of human galectin-1, GST fusion protein

Clone info. Expression vector of human galectin-1.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 1 cDNA
Depositor|Developer Nishi, Nozomu |
 
Remarks, protocol and/or map (pdf) RDB04217.pdf

Distribution information

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Terms and conditions for distribution With no restriction.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04217 pGEX-G1-1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G1-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04217).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13754_A6A4p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pJB8-Forward (Pr0084)
Region: insert, thrombin site, GST 3'
Sequence file: RDB13754_A6A4c.seq check
>04217_13754_A9HtF_JB8-Forward_A06_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGCT GCGCCCCGNC NCCCGCCAAC ACCCGCTGAN
   61 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC
  121 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGATCGTC
  181 AGTCAGTCAC GATGCGGCCG CTCGAGTCAG TCAAAGGCCA CACATTTGAT CTTGAAGTCA
  241 CCGTCAGCTG CCATGTAGTT GATGGCCTCC AGGTTGAGGC GGTTGGGGAA CTTGAATTCG
  301 TATCCATCTG GCAGCTTGAC GGTCAGGTTG GCCTGGTCGA AGGTGATGCA CACCTCTGCA
  361 ACACTTCCAG GCTGGAAGGG AAAGACAGCC TCCCGCTGCT CGGTCCCCCA GGCCCCGCCG
  421 TCCTTGCTGT TGCACACGAT GGTGTTGGCG TCGCCGTGGG CGTTGAAGCG AGGGTTGAAG
  481 TGCAGGCACA GGTTGTTGCT GTCTTTGCCC AGGTTCAGCA CGAAGCTCTT AGCGTCAGGA
  541 GCCACCTCGC CTCGCACTCG AAGGCACTCT CCAGGTTTGA GATTCAGGTT GCTGGCGACC
  601 AGACCACAAG CCATGGATCC ACGCGGAACC AGATCCGATT TTGGAGGATG GTCGCCACCA
  661 CCAAACGTGG CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA TTTCAAGTAC
  721 TTATCAATTT GTGGGATAGC TTCNATACGT TTTTTAAAAC AAACTAATTT TGGGAACGCA
  781 TCCAGGCACA TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA CATGAAGTCA
  841 GGATGGGTTA CATGATCACC ATTTAAATAT GTTTTATGAC ATANNCGATC TTCGAACATT
901 TTCAGCNTTT CNNNGTAGCT NGCTN
//
Primer: pUC-M13_-100 (Pr0310)
Region: lacZ alpha, lacI gene 3'
Sequence file: RDB13754_A6A4d.seq check
>04217_13754_A9HtF_pUC-M13_-100_B06_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GGNNNGCNNG GNNTTTCCCN GTCACGACNT TGTAAAACGA
   61 CGGCCAGTGA ATCCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
  121 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
  181 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
  241 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
  301 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
  361 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
  421 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
  481 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
  541 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
  601 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
  661 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
  721 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CNNNNNGCTC
  781 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAAATAAT ACTGTTGATG GGTGTCTGGT
  841 CAGAGACATC NAAGAAAATA ACGNCNGGAA CATTAGTGCA NGCAGCTTCC ACAGCAATGG
901 CNTCCTGN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]