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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G1-1a

Bacteria expression vector of human galectin-1, GST fusion protein

Catalog number RDB04217
Resource name pGEX-G1-1a
Clone info. Expression vector of human galectin-1.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 1 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank
External Database
Human lectin, galactoside-binding, soluble, 1
          Reference sequence
            

          Distribution information

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          RDB04217 pGEX-G1-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB04217.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13754_A6A4p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pJB8-Forward
          Region: insert, thrombin site, GST 3'
          Sequence file: RDB13754_A6A4c.seq check
          >04217_13754_A9HtF_JB8-Forward_A06_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGCT GCGCCCCGNC NCCCGCCAAC ACCCGCTGAN
             61 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC
            121 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGATCGTC
            181 AGTCAGTCAC GATGCGGCCG CTCGAGTCAG TCAAAGGCCA CACATTTGAT CTTGAAGTCA
            241 CCGTCAGCTG CCATGTAGTT GATGGCCTCC AGGTTGAGGC GGTTGGGGAA CTTGAATTCG
            301 TATCCATCTG GCAGCTTGAC GGTCAGGTTG GCCTGGTCGA AGGTGATGCA CACCTCTGCA
            361 ACACTTCCAG GCTGGAAGGG AAAGACAGCC TCCCGCTGCT CGGTCCCCCA GGCCCCGCCG
            421 TCCTTGCTGT TGCACACGAT GGTGTTGGCG TCGCCGTGGG CGTTGAAGCG AGGGTTGAAG
            481 TGCAGGCACA GGTTGTTGCT GTCTTTGCCC AGGTTCAGCA CGAAGCTCTT AGCGTCAGGA
            541 GCCACCTCGC CTCGCACTCG AAGGCACTCT CCAGGTTTGA GATTCAGGTT GCTGGCGACC
            601 AGACCACAAG CCATGGATCC ACGCGGAACC AGATCCGATT TTGGAGGATG GTCGCCACCA
            661 CCAAACGTGG CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA TTTCAAGTAC
            721 TTATCAATTT GTGGGATAGC TTCNATACGT TTTTTAAAAC AAACTAATTT TGGGAACGCA
            781 TCCAGGCACA TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA CATGAAGTCA
            841 GGATGGGTTA CATGATCACC ATTTAAATAT GTTTTATGAC ATANNCGATC TTCGAACATT
          901 TTCAGCNTTT CNNNGTAGCT NGCTN
          //
          Primer: pUC-M13_-100
          Region: lacZ alpha, lacI gene 3'
          Sequence file: RDB13754_A6A4d.seq check
          >04217_13754_A9HtF_pUC-M13_-100_B06_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGNNNGCNNG GNNTTTCCCN GTCACGACNT TGTAAAACGA
             61 CGGCCAGTGA ATCCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
            121 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
            181 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
            241 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
            301 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
            361 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
            421 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
            481 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
            541 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
            601 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
            661 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
            721 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CNNNNNGCTC
            781 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAAATAAT ACTGTTGATG GGTGTCTGGT
            841 CAGAGACATC NAAGAAAATA ACGNCNGGAA CATTAGTGCA NGCAGCTTCC ACAGCAATGG
          901 CNTCCTGN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl