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pOGN (#RDB15987)

Porcine cDNA for osteoglycin/mimecan (OGN).

Alternative name Porcine osteoglycin/mimecan
Clone info. Porcine cDNA for osteoglycin/mimecan (OGN) in pGEM-T Easy vector.
Vector backbone pGEM-T Easy (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name sus scrofa (porcine) OGN cDNA
Reference of insert sequence LC060063 (DDBJ accession number)
Depositor|Developer Nishi, Nozomu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017)。

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RDB15987 pOGN DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pOGN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15987).

Reference section:

Itoh, A., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15987_A8Fip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 ()
Region: 5'UTR,insert5'
Sequence file: RDB15987_A8Fia.seq check
>D05595A5-2_A8Fi_M13_-40_F01_06_ABI08.ab1
    1 AGCCAGGGAT TGTAATACGA CTCACTATAG GGCGAATTGG GCCCGACGTC GCATGCTCCC
   61 GGCCGCCATG GCGGCCGCGG GAATTCGATT TTGTCCCACA GTCAGCAGGC CACTAGCTTA
  121 TTAACTTCCA GACACCTTAA TTTTTGCTAA AATGAAGACT CTGCAATCTA CACTTCTCTT
  181 GTTCCTGTTT GTGCCTCTGA TAAAGCCAGC ACCACCATCT CAGCAGGACT CTCGCATTAT
  241 CTCTGATTAT GGAACAAATA ATTTTGAAGA AACCTTATTT AGCCAAGATT ATGAGGATAA
  301 ATACCTGGAT GGAAAAAGTA TTAAGGAAAA AGAGGCTATC AGAATACCTG ATGAGAAAAG
  361 TTTTGAATTA CAAAAAGACG AGAGTACAAT ACCATTATCC CCCAAGAAAG AAAACGATGA
  421 AATGCCCACA TGTCTGCTGT GTGTTTGTTT AAGTGGCTCT GTATACTGTG AAGAAATTGA
  481 CATTAAAGCT GTACCACCCT TGCCAAAGGA ATCAGCCTAT CTTTATGCAC GATTCAACAA
  541 AATTAAAAAG TTGACTGCCA AAGATTTTGC AGATATACCT AACTTAAGAC GACTTGATTT
  601 TACGGGAAAT TTGATTGAAG ATATAGAAGA TGGCACTTTC TCAAAACTCT CTCTGTTAGA
  661 AGAACTTATA CTAGCTGACA ATCAACTACT GAAACTTCCA GTTCTCCCTC CTAAGCTTAC
  721 GTTATTTAAT GCAAAACGCA ACAAAATCAA GAGTAGGGGA ATAAAAGCAA ATGCATTCAA
  781 AAAATTGAAT AAACTCTCCT TCCTCTACCT GGACCACAAT GCCCTGGAAT CCGTGCCTCT
  841 TAATTTACCA GAAAGTCTGC GTATAATTCA TCTTCAGGGT AACAACATAA CTTCAATTAC
  901 AGATGACACA TTCTGCAAGG GCTAATGACA CCAGTTATAT TCGGGATCAC ATTGAAGAGA
  961 TACGCTTGGG AAGATAATCC CATCATCCTG GGAAAATATC CCAACAGTTT TATCTGCTTT
 1021 AAAAAGACTG CCCTATAGGG TCATACATCT AGTCACTATC ACACAACATA TAAGCTAAAG
 1081 TACACACATA ACTTATAATC TGTCTCAATT CACTTAGTTG ATTTCGCATC GCCTGGCAGT
1141 TCGGACCAAT ATATTTGGGG GAGAGAAGGC CTTTCCTCCC A
//
Primer: ColE1ori_F ()
Region: ori,3'UTR,insert3'
Sequence file: RDB15987_A8Fib.seq check
>D05595A5-2_A8Fi_ColE1ori_F_C03_09_ABI24.ab1
    1 GGTGGCAGTC GGATCGCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
   61 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
  121 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
  181 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
  241 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
  301 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC
  361 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
  421 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA
  481 TGATTACGCC AAGCTATTTA GGTGACACTA TAGAATACTC AAGCTATGCA TCCAACGCGT
  541 TGGGAGCTCT CCCATATGGT CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTGAGACA
  601 GATTATAAGT ATGTGTGTAC TTTAGCTTAT ATGTTGTGTT GATAGTGACT AGATGTATGA
  661 CCCTATAGGC AGTCTTTTTA AGCAGATAAA ACTGTTGGGA TATTTTCCCA GGATGATGGG
  721 ATTATCTTCC AAGCGTATCT CTTCAATGTG ATCCCGAATA TAACTGGTGT CATTAGCCTT
  781 GCAGAATGTG TCATCTGTAA TTGAAGTTAT GTTGTTACCC TGAAGATGAA TTATACGCAG
  841 ACTTTCTGGT AAATTAAGAG GCACGGATTC CAGGGCATTG TGGTCCAGGT AGAGGAAGGA
  901 GAGTTTATTC AATTTTTTGA ATGCAATTTG CTTTTATTCC CCCTACTCTT GATTTTTGTT
  961 GCGTTTTGCA TTAAATAACG TAAGCTTAGA GGAGAACTGG AAGTTTCAGT AGTTGATTGT
 1021 CAGCTAGTAT AGTTCTTTCT TAACAGAGGA GGAGTTTTGA GAAAGTTGGC CATTCCTCCT
1081 ATGAGTGCGG TTGCGATCAA AATCCGTAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. [link to RRC of NBRP]