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| Catalog # | Name of clone | Running title | Publication |
| RDB20680 | pCMV-EGFP-Cav-1 | Expression vector of human Caveolin-1. EGFP was fused to the N terminus. | Kuwashima, Y., TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane. Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. (original). |
| RDB20678 | pFN21A HaloTag-TRPV4 (R616Q) | Expression vector of human TRPV4 (R616Q). HaloTag(R) 7 was fused to the N terminus. | Kuwashima, Y., TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane. Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. (original). |
| RDB20677 | pFN21A HaloTag-TRPV4 (WT) | Expression vector of human TRPV4. HaloTag(R) 7 was fused to the N terminus. | Kuwashima, Y., TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane. Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. (original). |
| RDB20676 | pFN21A-TRPV4 (WT) | Expression vector of human TRPV4. | Kuwashima, Y., TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane. Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. (original). |
| RDB20674 | pCMV-EGFRvIII-Halo | Expression vector of human EGFRvIII (Del30-297). HaloTag(R) 7 was fused to the C terminus. | Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. (original). |
| RDB20673 | pCMV-EGFR(3RN)-Halo | Expression vector of human mutant EGFR(3RN, R645N/R646N/R647N). HaloTag(R) 7 was fused to the C terminus. | Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. (original). |
| RDB20672 | pCMV-EGFR(WT)-Halo | Expression vector of human wild-type EGFR. HaloTag(R) 7 was fused to the C terminus. | Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. (original). |
| RDB20671 | pCMV-Halo-TubbyC | Expression vector of the Tubby C-terminal domain, which interacts with PI(4,5)P2. HaloTag(R) 7 was fused to the N terminus. | Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. (original). |
| RDB20667 | DEST40/PAmCherry-GRP1-PH | Expression vector of the human GRP1 PH domain, which interacts with PI(3,4,5)P3 tagged with PAmCherry at the N terminus. | Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. (original). |
| RDB20666 | DEST40/EGFR-rsKame | Expression vector of human EGFR tagged with rsKame at the N terminus. | Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. (original). |
| RDB19219 | pTRE-SaSMaseC | Mammalian expression vector of Staphylococcus aureus sphingomyelinase C (SMase C), Tet-On/Off Advanced IRES mCherry system. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19218 | pAcGFP-PMP2 | Mammalian expression vector of human PMP2 fused with AcGFP at C-terminus. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19217 | pDEST/AcGFP-synaptojanin | Mammalian expression vector of human synaptojanin fused with AcGFP at N-terminus. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19216 | pDEST/mCherry-synaptojanin | Mammalian expression vector of human synaptojanin fused with mCherry at N-terminus. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19215 | pET28/His6-NT-Lys | Expression vector of His-tagged nontoxic Lysenin for E. coli. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19214 | pET28/His6-EGFP-Lact-C2 | Expression vector of His and EGFP-tagged C2 domain of bovine Lactadherin for E. coli. Phosphatidylserine (PS)-specific probe. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19213 | pTRE-TRPV4 | Mammalian expression vector of human TRPV4, Tet-On/Off Advanced IRES mCherry system. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19212 | pTRE-PMP2 | Mammalian expression vector of human PMP2, Tet-On/Off Advanced IRES mCherry system. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19211 | pHalo-PLC delta-PH | Mammalian expression vector of HaloTag(R) 7-tagged PH domain of human phospholipase C delta 1 (PLC delta 1). | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19210 | pHalo-PMP2(I43N) | Mammalian expression vector of HaloTag(R) 7-tagged human PMP2 (I43N). | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19209 | pHalo-PMP2 | Mammalian expression vector of HaloTag(R) 7-tagged human PMP2. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19208 | pHalo-NT-Lys | Mammalian expression vector of HaloTag(R) 7-tagged nontoxic Lysenin. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19207 | pET28/His6-FABP4 | Expression vector of His-tagged human FABP4 for E. coli. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19206 | pET28/His6-FABP3 | Expression vector of His-tagged human FABP3 for E. coli. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19205 | pET28/His6-PMP2(R127Q) | Expression vector of His-tagged human PMP2 (R127Q) for E. coli. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19204 | pET28/His6-PMP2(I43N) | Expression vector of His-tagged human PMP2 (I43N) for E. coli. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB19203 | pET28/His6-PMP2 | Expression vector of His-tagged human PMP2 for E. coli. | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original). [link to RRC of NBRP] |
| RDB18944 | pDEST/mCherry-D4 | Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 with mCherry. | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original). [link to RRC of NBRP] |
| RDB18943 | pDEST/mCherry-NT-Lys | Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic lysenin with mCherry. | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original). [link to RRC of NBRP] |
| RDB16767 | pDEST/mCherry-CeRhoA | Expression vector of C. elegans RhoA. | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original). [link to RRC of NBRP] |
| RDB16766 | pDEST/mCherry-TubbyC | Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C). | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original). [link to RRC of NBRP] |
| RDB16765 | pDEST/mCherry-PIP5KB | Expression vector of human phosphatidylinositol 4-phosphate 5-kinase beta (PIP5K beta). | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original). [link to RRC of NBRP] |
| RDB16764 | pDEST/mCherry-PLCD1-PH | Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1). | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original). [link to RRC of NBRP] |
| RDB14701 | pDEST/mCherry-Rab18 S22N | Expression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original). [link to RRC of NBRP] |
| RDB14700 | pDEST/mCherry-Rab18 Q67L | Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original). [link to RRC of NBRP] |
| RDB14699 | pDEST/mCherry-Rab18 | Expression vector of human Rab18, wild type, fused with mCherry. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original). [link to RRC of NBRP] |
| RDB14698 | pSpCas9(BB)-2A-puro-ACAT2 sgRNA | Expression vector of sgRNA for targeting human ACAT2 and Cas9. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original). [link to RRC of NBRP] |
| RDB14697 | pSpCas9(BB)-2A-puro-ACAT1 sgRNA | Expression vector of sgRNA for targeting human ACAT1 and Cas9. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original). [link to RRC of NBRP] |
| RDB14301 | pET28/His6-EGFP-Nakanori | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. | Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. (original). [link to RRC of NBRP] |
| RDB14300 | pET28/His6-mCherry-D4 | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens. | Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. (original). [link to RRC of NBRP] |
| RDB13964 | pET28/EryA-EGFP-His6 | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA. | Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. (original). [link to RRC of NBRP] |
| RDB13963 | pET28/Oly-EGFP-His6 | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly. | Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. (original). [link to RRC of NBRP] |
| RDB13962 | pET28/PlyA2-EGFP-His6 | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2. | Bhat, H.B., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047. (original). [link to RRC of NBRP] |
| RDB13961 | pET28/His6-EGFP-D4 | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens. | Shimada, Y., The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains. Eur. J. Biochem. 269 (24): 6195-6203 (2002). PMID 12473115. (original). [link to RRC of NBRP] |
| RDB13960 | pQE30/His6-mRFP-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin. | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. (original). [link to RRC of NBRP] |
| RDB13959 | pQE30/His6-Venus-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin. | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. (original). [link to RRC of NBRP] |
| RDB13958 | pMAL/MBP-lysenin | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin. | Kiyokawa, E., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631. (original). [link to RRC of NBRP] |
| RDB13501 | pCAG/tdiRFP-CAAX | Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein. | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original). [link to RRC of NBRP] |
| RDB13499 | pET28/His6-mCherry-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original). [link to RRC of NBRP] |
| RDB13498 | pET28/His6-EGFP-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original). [link to RRC of NBRP] |
| RDB13497 | pET28/EqtII(8-69)-EGFP-His6 | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II. | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original). [link to RRC of NBRP] |
| RDB13496 | pET28/EqtII-EGFP-His6 | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original). [link to RRC of NBRP] |
2025.03.05
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