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Mitsuhiro Abe's Resource

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Catalog #Name of cloneRunning titlePublication
RDB13496pET28/EqtII-EGFP-His6Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13497pET28/EqtII(8-69)-EGFP-His6Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13498pET28/His6-EGFP-NT-LysMarker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13499pET28/His6-mCherry-NT-LysMarker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13501pCAG/tdiRFP-CAAXMarker of plasma membrane. Mammalian expression plasmid of CAAX box protein.Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. (original).
RDB13958pMAL/MBP-lyseninMarker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.Kiyokawa, E., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631. (original).
RDB13959pQE30/His6-Venus-NT-LysMarker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. (original).
RDB13960pQE30/His6-mRFP-NT-LysMarker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. (original).
RDB13961pET28/His6-EGFP-D4Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens.Shimada, Y., The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains. Eur. J. Biochem. 269 (24): 6195-6203 (2002). PMID 12473115. (original).
RDB13962pET28/PlyA2-EGFP-His6Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.Bhat, H.B., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047. (original).
RDB13963pET28/Oly-EGFP-His6Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. (original).
RDB13964pET28/EryA-EGFP-His6Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. (original).
RDB14300pET28/His6-mCherry-D4Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens.Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. (original).
RDB14301pET28/His6-EGFP-NakanoriMarker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. (original).
RDB14697pSpCas9(BB)-2A-puro-ACAT1 sgRNAExpression vector of sgRNA for targeting human ACAT1 and Cas9.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14698pSpCas9(BB)-2A-puro-ACAT2 sgRNAExpression vector of sgRNA for targeting human ACAT2 and Cas9.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14699pDEST/mCherry-Rab18Expression vector of human Rab18, wild type, fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14700pDEST/mCherry-Rab18 Q67LExpression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB14701pDEST/mCherry-Rab18 S22NExpression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. (original).
RDB16764pDEST/mCherry-PLCD1-PHExpression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1).Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original).
RDB16765pDEST/mCherry-PIP5KBExpression vector of human phosphatidylinositol 4-phosphate 5-kinase beta (PIP5K beta).Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original).
RDB16766pDEST/mCherry-TubbyCExpression vector of human Tubby proteins C-terminal tubby domain (Tubby C).Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original).
RDB16767pDEST/mCherry-CeRhoAExpression vector of C. elegans RhoA.Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original).
RDB18943pDEST/mCherry-NT-LysMarker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic lysenin with mCherry.Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original).
RDB18944pDEST/mCherry-D4Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 with mCherry.Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. (original).
RDB19203pET28/His6-PMP2 Expression vector of His-tagged human PMP2 for E. coli.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19204pET28/His6-PMP2(I43N) Expression vector of His-tagged human PMP2 (I43N) for E. coli.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19205pET28/His6-PMP2(R127Q) Expression vector of His-tagged human PMP2 (R127Q) for E. coli.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19206pET28/His6-FABP3 Expression vector of His-tagged human FABP3 for E. coli.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19207pET28/His6-FABP4 Expression vector of His-tagged human FABP4 for E. coli.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19208pHalo-NT-Lys Mammalian expression vector of HaloTag(R) 7-tagged nontoxic Lysenin.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19209pHalo-PMP2 Mammalian expression vector of HaloTag(R) 7-tagged human PMP2.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19210pHalo-PMP2(I43N) Mammalian expression vector of HaloTag(R) 7-tagged human PMP2 (I43N).Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19211pHalo-PLC delta-PHMammalian expression vector of HaloTag(R) 7-tagged PH domain of human phospholipase C delta 1 (PLC delta 1).Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19212pTRE-PMP2 Mammalian expression vector of human PMP2, Tet-On/Off Advanced IRES mCherry system.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19213pTRE-TRPV4 Mammalian expression vector of human TRPV4, Tet-On/Off Advanced IRES mCherry system.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19214pET28/His6-EGFP-Lact-C2 Expression vector of His and EGFP-tagged C2 domain of bovine Lactadherin for E. coli. Phosphatidylserine (PS)-specific probe.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19215pET28/His6-NT-Lys Expression vector of His-tagged nontoxic Lysenin for E. coli.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19216pDEST/mCherry-synaptojanin Mammalian expression vector of human synaptojanin fused with mCherry at N-terminus.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19217pDEST/AcGFP-synaptojanin Mammalian expression vector of human synaptojanin fused with AcGFP at N-terminus.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19218pAcGFP-PMP2 Mammalian expression vector of human PMP2 fused with AcGFP at C-terminus.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).
RDB19219pTRE-SaSMaseC Mammalian expression vector of Staphylococcus aureus sphingomyelinase C (SMase C), Tet-On/Off Advanced IRES mCherry system.Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. (original).

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2023.03.15

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