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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-NT-Lys

Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic lysenin with mCherry.

Catalog number RDB18943
Resource name pDEST/mCherry-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic (NT) lysenin fused with mCherry at N-terminus.
Comment Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry. Expression was confirmed by the depositor with microscopy.
Vector backbone pcDNA-DEST40 (plasmid)
Size of vector backbone 7.1 kb
Selectable markers Ampicillin
Gene/insert name Eisenia fetida lysenin cDNA
Depositor|Developer Abe, Mitsuhiro |
Other clones in our bank
External Database
Eisenia fetida lysenin
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020).
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18943 pDEST/mCherry-NT-Lys DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pDEST/mCherry-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18943).

            Reference section:

            Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18943_B1Ctp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward (Pr0016)
            Region: CMV pro, insert 5'(mCherry)
            Sequence file: RDB18943_B1Cta.seq check
            >D07782A1_B1Ct_2_CMV_Forward_C07_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNTTGN NGCNNATGGG CGGTANGCGT GTACGGTGGG AGGTCTATAT
               61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
              121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT GGTGAGCAAG
              181 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG
              241 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG
              301 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC
              361 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC
              421 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC
              481 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC
              541 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG
              601 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC
              661 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA NGTCAAGACC
              721 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG
              781 GACATCACCT CCCACAACGA GGANTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC
              841 CGCCACTCCA CCGGCGGCNT GGACGANCTG TACAAGAAGC TTATAATCCT NNGAAAAACA
              901 GAAATTCGAA TTNAGCATGC AGAAAGGAAN GANTACNTGA NAGTCNNTTC NNNAAAANNT
            961 NGGNCANCTG CNNNNNNNNN N
            //
            Primer: bGH_rev3 (Pr0885)
            Region: bGH pA, insert 3'(NT-Lys,mCherry)
            Sequence file: RDB18943_B1Ctb.seq check
            >D07782A1_B1Ct_2_bGH_rev3_E07_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGGNN GNNGGNNNNN NANAGATGGC TGGCAACTAG AAGGCACAGT
               61 CGAGGCTGAT CAGCGGGTTT AAACCTCGAG TTAACCAACC ACTTCCAAAA TCCACTTGTC
              121 TTCACGTTTG TCCAGACAGT ACACGTTTGT TGCCGGTCCA TCATCGTAGC ACAGCCCACT
              181 GCGAGTGAAG TACTTATTCA TGAAGGTGAC TACGTCACCA TGACGAAGAG GCAATGACTT
              241 ATTGATTGCC CAGCGCTGTT TCGGATTATC AGTACCCTGA TCAAAGTAGA TTCCTCCTTG
              301 ATCAGAGGAA TAGGCATACT CATAGCCCGA ATACATGGTG TTCAGCGTTT TAATTCGAAA
              361 TCCCCCCCAA TCTTCATAGA GCACAAACTT GAAAAGTTTG CTATGTCCAA GAGTTGCAGC
              421 TGGCCAACTT TTTCTTGAAA CGACTGTCAT GTACTCCTTC CTTTCTGCAT GCTTAATTCG
              481 AATTTCTGTT TTTCCAAGGA TTATAAGCTT CTTGTACAGC TCGTCCATGC CGCCGGTGGA
              541 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
              601 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
              661 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
              721 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGANGANG CCTCCCAGCC
              781 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGANGGGAAG TTGGTGCCGC GCANCTTCAC
              841 CTTGTAGATG AACTCGCCGT CCTGCANGGN NGANTCCTGG GNCACGGNCA NCACGCCGCC
              901 NNNCTCNAAN TTCATCACGC GCNCCNNCTT GNANCCNNNN GGGNNNNANN NCTTNNNGTA
            961 NNNNNNANGN CGGNGGGGNG NNTNNNNNNN NNCNNNNNNN NNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori, NeoR
            Sequence file: RDB18943_B1Ctc.seq check
            >D07782A1_B1Ct_2_SV40pro_ori_F_F07_16_ABI24.ab1
                1 NNNNNNNNNN NNGNNGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT
               61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
              121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
              181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
              241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
              301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
              361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
              421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
              481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
              541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
              601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
              661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC
              721 TGCTTGCCGA ATATCATGGT GGAAAATGNN CGCTTTTCTG GATTCATCGA CTGTGGCCGG
              781 CTGGGTGTGG CGGANCGCTA TCAGGACATA NCGTTGGCTA CCCGTGNNAT TGCTGAAGAN
              841 CTTGGCGGCG AATGNNCTGA NCGCTTCCTC GNGCTTTACN GNATCGCCGC TNCCGANTCG
              901 CAGCGCATCG CCNNCTATCG CCTTCTTGNN NANTNCNTCT NNNCGGNNNT NNGGGNNNNC
            961 GCGNANNGAN NGANNNNNCN NNNNCNNANN CNNNNCNNNN NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl