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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-NT-Lys (#RDB18943)

Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic lysenin with mCherry.

Clone info. Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic (NT) lysenin fused with mCherry at N-terminus.
Comment Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry. Expression was confirmed by the depositor with microscopy.
Vector backbone pcDNA-DEST40 (plasmid)
Size of vector backbone 7.1 kb
Selectable markers Ampicillin
Gene/insert name Eisenia fetida lysenin cDNA
Depositor|Developer Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18943 pDEST/mCherry-NT-Lys DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18943).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18943_B1Ctp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'(mCherry)
Sequence file: RDB18943_B1Cta.seq check
>D07782A1_B1Ct_2_CMV_Forward_C07_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTGN NGCNNATGGG CGGTANGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT GGTGAGCAAG
  181 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG
  241 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG
  301 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC
  361 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC
  421 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC
  481 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC
  541 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG
  601 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC
  661 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA NGTCAAGACC
  721 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG
  781 GACATCACCT CCCACAACGA GGANTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC
  841 CGCCACTCCA CCGGCGGCNT GGACGANCTG TACAAGAAGC TTATAATCCT NNGAAAAACA
  901 GAAATTCGAA TTNAGCATGC AGAAAGGAAN GANTACNTGA NAGTCNNTTC NNNAAAANNT
961 NGGNCANCTG CNNNNNNNNN N
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA, insert 3'(NT-Lys,mCherry)
Sequence file: RDB18943_B1Ctb.seq check
>D07782A1_B1Ct_2_bGH_rev3_E07_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGGNN GNNGGNNNNN NANAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACCTCGAG TTAACCAACC ACTTCCAAAA TCCACTTGTC
  121 TTCACGTTTG TCCAGACAGT ACACGTTTGT TGCCGGTCCA TCATCGTAGC ACAGCCCACT
  181 GCGAGTGAAG TACTTATTCA TGAAGGTGAC TACGTCACCA TGACGAAGAG GCAATGACTT
  241 ATTGATTGCC CAGCGCTGTT TCGGATTATC AGTACCCTGA TCAAAGTAGA TTCCTCCTTG
  301 ATCAGAGGAA TAGGCATACT CATAGCCCGA ATACATGGTG TTCAGCGTTT TAATTCGAAA
  361 TCCCCCCCAA TCTTCATAGA GCACAAACTT GAAAAGTTTG CTATGTCCAA GAGTTGCAGC
  421 TGGCCAACTT TTTCTTGAAA CGACTGTCAT GTACTCCTTC CTTTCTGCAT GCTTAATTCG
  481 AATTTCTGTT TTTCCAAGGA TTATAAGCTT CTTGTACAGC TCGTCCATGC CGCCGGTGGA
  541 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
  601 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
  661 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
  721 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGANGANG CCTCCCAGCC
  781 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGANGGGAAG TTGGTGCCGC GCANCTTCAC
  841 CTTGTAGATG AACTCGCCGT CCTGCANGGN NGANTCCTGG GNCACGGNCA NCACGCCGCC
  901 NNNCTCNAAN TTCATCACGC GCNCCNNCTT GNANCCNNNN GGGNNNNANN NCTTNNNGTA
961 NNNNNNANGN CGGNGGGGNG NNTNNNNNNN NNCNNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR
Sequence file: RDB18943_B1Ctc.seq check
>D07782A1_B1Ct_2_SV40pro_ori_F_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNGNNGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
  121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT
  181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC
  241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC
  301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC
  361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
  421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG
  481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC
  541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT
  601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
  661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC
  721 TGCTTGCCGA ATATCATGGT GGAAAATGNN CGCTTTTCTG GATTCATCGA CTGTGGCCGG
  781 CTGGGTGTGG CGGANCGCTA TCAGGACATA NCGTTGGCTA CCCGTGNNAT TGCTGAAGAN
  841 CTTGGCGGCG AATGNNCTGA NCGCTTCCTC GNGCTTTACN GNATCGCCGC TNCCGANTCG
  901 CAGCGCATCG CCNNCTATCG CCTTCTTGNN NANTNCNTCT NNNCGGNNNT NNGGGNNNNC
961 GCGNANNGAN NGANNNNNCN NNNNCNNANN CNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]