Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic lysenin with mCherry.
Clone info. | Marker of sphingomyelin in cell membrane. Expression vector of common brandling worm non-toxic (NT) lysenin fused with mCherry at N-terminus. |
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Comment | Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry. Expression was confirmed by the depositor with microscopy. |
Vector backbone | pcDNA-DEST40 (plasmid) |
Size of vector backbone | 7.1 kb |
Selectable markers | Ampicillin |
Gene/insert name | Eisenia fetida lysenin cDNA |
Depositor|Developer | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020). |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020)。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18943 | pDEST/mCherry-NT-Lys | DNA solution |
Materials & Methods section:
The pDEST/mCherry-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18943). |
Reference section:
Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18943_B1Ctp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5'(mCherry) Sequence file: RDB18943_B1Cta.seq ![]() |
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>D07782A1_B1Ct_2_CMV_Forward_C07_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNTTGN NGCNNATGGG CGGTANGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC 121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT GGTGAGCAAG 181 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG 241 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG 301 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC 361 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC 421 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC 481 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC 541 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG 601 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC 661 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA NGTCAAGACC 721 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG 781 GACATCACCT CCCACAACGA GGANTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC 841 CGCCACTCCA CCGGCGGCNT GGACGANCTG TACAAGAAGC TTATAATCCT NNGAAAAACA 901 GAAATTCGAA TTNAGCATGC AGAAAGGAAN GANTACNTGA NAGTCNNTTC NNNAAAANNT 961 NGGNCANCTG CNNNNNNNNN N // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA, insert 3'(NT-Lys,mCherry) Sequence file: RDB18943_B1Ctb.seq ![]() |
>D07782A1_B1Ct_2_bGH_rev3_E07_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGNN GNNGGNNNNN NANAGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGGGTTT AAACCTCGAG TTAACCAACC ACTTCCAAAA TCCACTTGTC 121 TTCACGTTTG TCCAGACAGT ACACGTTTGT TGCCGGTCCA TCATCGTAGC ACAGCCCACT 181 GCGAGTGAAG TACTTATTCA TGAAGGTGAC TACGTCACCA TGACGAAGAG GCAATGACTT 241 ATTGATTGCC CAGCGCTGTT TCGGATTATC AGTACCCTGA TCAAAGTAGA TTCCTCCTTG 301 ATCAGAGGAA TAGGCATACT CATAGCCCGA ATACATGGTG TTCAGCGTTT TAATTCGAAA 361 TCCCCCCCAA TCTTCATAGA GCACAAACTT GAAAAGTTTG CTATGTCCAA GAGTTGCAGC 421 TGGCCAACTT TTTCTTGAAA CGACTGTCAT GTACTCCTTC CTTTCTGCAT GCTTAATTCG 481 AATTTCTGTT TTTCCAAGGA TTATAAGCTT CTTGTACAGC TCGTCCATGC CGCCGGTGGA 541 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT 601 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT 661 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT 721 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGANGANG CCTCCCAGCC 781 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGANGGGAAG TTGGTGCCGC GCANCTTCAC 841 CTTGTAGATG AACTCGCCGT CCTGCANGGN NGANTCCTGG GNCACGGNCA NCACGCCGCC 901 NNNCTCNAAN TTCATCACGC GCNCCNNCTT GNANCCNNNN GGGNNNNANN NCTTNNNGTA 961 NNNNNNANGN CGGNGGGGNG NNTNNNNNNN NNCNNNNNNN NNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR Sequence file: RDB18943_B1Ctc.seq ![]() |
>D07782A1_B1Ct_2_SV40pro_ori_F_F07_16_ABI24.ab1 1 NNNNNNNNNN NNGNNGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT 61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 721 TGCTTGCCGA ATATCATGGT GGAAAATGNN CGCTTTTCTG GATTCATCGA CTGTGGCCGG 781 CTGGGTGTGG CGGANCGCTA TCAGGACATA NCGTTGGCTA CCCGTGNNAT TGCTGAAGAN 841 CTTGGCGGCG AATGNNCTGA NCGCTTCCTC GNGCTTTACN GNATCGCCGC TNCCGANTCG 901 CAGCGCATCG CCNNCTATCG CCTTCTTGNN NANTNCNTCT NNNCGGNNNT NNGGGNNNNC 961 GCGNANNGAN NGANNNNNCN NNNNCNNANN CNNNNCNNNN NNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP] |
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