Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/His6-EGFP-D4

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimada, Y., Eur J Biochem. 269 (24): 6195-6203, 2002.)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimada, Y., Eur J Biochem. 269 (24): 6195-6203, 2002.)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13961_A6Btp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R Region: T7 pro, lacO, RBS, 6xHis, thrombin site, EGFP, D4 5' Sequence file: RDB13961_A6Bta.seq
>D03144A4_A6Bt_1_pBR 322 BamHI R_B04_04.ab1     1 TCCGACATAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA    61 TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCATGGGCA GCAGCCATCA TCATCATCAT   121 CACAGCAGCG GCCTGGTGCC GCGCGGCAGC CATATGGCTA GAGTGAGCAA GGGCGAGGAG   181 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG   241 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC   301 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC   361 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC   421 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC   481 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG   541 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC   601 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG   661 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC   721 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC   781 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC   841 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGCTA GCAAGGGAAA AATAAACTTA   901 GATCATAGTG GAGCCTATGT TGCACAGTTT GAAGTAGCCT GGGGATGAAG TTTCATATGA   961 CAAGAATGGA AATGAAAGTT TTAACTCATA AAACATGGGA TGGGAAATTA TCAAGATAAA  1021 ACAGCTCACT AATTCACAGT AATACCCTCT GAGGCTTAAT TGCAGAAATA ATAGAATAAG 1081 CCAGAGGACT GTACAGGTCC TTGGCTTTGG GGGCAAATGG TTTT //
Primer: T7 terminator Region: D4, EGFP 3' Sequence file: RDB13961_A6Btb.seq
>D03144A4_A6Bt_1_T7Terminator_A04_01.ab1     1 CAATGCATCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGCTCG    61 AGTGCGGCCG CAAGCTTGTC GACGGAGCTC GAATTCGAGC TCGGTACCCT ATGAATCTAA   121 TACATGTAAA CCAAATTTAT TGTATAGTAA TGTCCAAAAC AAATCTAATA TTTACTTTAT   181 TATAACTCTA AAATATGAAA TAAAAAACTG ATTTTAGACT AGCTAAAATC AGTTTTTACT   241 TTAGTTTAAT TGTAAGTAAT ACTAGATCCA GGGTATAAAG TTGTTCCCCA TATTGAAACA   301 TTTATATTAT TTGTTAATGG AACATCATAT TCACTTATAA CATCTCTCCA CCATTCCCAA   361 GCAAGGCCTG TACACTCTCT TGCTTTTATT CTTATATTTC TTGCATTAGC TTCAAGAGGT   421 ATTACTGTTG AATAGTGAGC TGTTTTATCT TGATAATTTC CATCCCATGT TTTATGAGTT   481 AAAACTTCAT TTCCTTCTTT GTCATATGAA ACTTCATCCC AGGCTACTTC AAACTGTGCA   541 ACATAGGCTC CACTATGATC TAAGTTTATT TTTCCCTTGC TAGCCTTGTA CAGCTCGTCC   601 ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC   661 TCGTTGGGGT CTTTGCTCAG GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC 721 ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGC //
Primer: pGL3_RV4 Region: lacI Sequence file: RDB13961_A6Btc.seq
>D03144A4_A6Bt_1_pGL3_RV4_F12_18.ab1     1 GGGACGATGG GTTGAGGCTC TCAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC    61 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC   121 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG   181 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC   241 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT   301 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC   361 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC   421 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT   481 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT   541 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT   601 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC   661 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC   721 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC   781 ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC   841 CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG   901 TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT   961 GGAGTGGCAC GCCCATCAGC AACGACTGTT TGCCGCCAGT GTTGTGCCAC GCGTTGGGAT  1021 GTAATCCAGC TCGCAATCGC CGGCTCAACT TTTTCCCGCG TTTCGCAGAA CGTTGTCTGA 1081 CTGGTCACCC ACGCGGGGAA CT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl