Sequencing and PCR primers for primer information.
Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens.
| Catalog number | RDB13961 |
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| Resource name | pET28/His6-EGFP-D4 |
| Clone info. | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 (domain 4 of theta toxin; K363-N472) of C. perfringens. Expression was confirmed by the depositor. |
| Vector backbone | pET-28b (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Km^r |
| Gene/insert name | Clostridium perfringens pfoA cDNA Aequorea victoria GFP cDNA |
| Depositor | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimada, Y., Eur J Biochem. 269 (24): 6195-6203, 2002.) |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13961 | pET28/His6-EGFP-D4 | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Sphingolipid signaling pathway (English text) |
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| Featured content | Organelle marker (English text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13961_A6Btp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pBR 322 BamHI R | RDB13961_A6Bta.seq |
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>D03144A4_A6Bt_1_pBR 322 BamHI R_B04_04.ab1 1 TCCGACATAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA 61 TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCATGGGCA GCAGCCATCA TCATCATCAT 121 CACAGCAGCG GCCTGGTGCC GCGCGGCAGC CATATGGCTA GAGTGAGCAA GGGCGAGGAG 181 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG 241 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 301 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC 361 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 421 GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC 481 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 541 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC 601 AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG 661 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 721 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC 781 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 841 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGCTA GCAAGGGAAA AATAAACTTA 901 GATCATAGTG GAGCCTATGT TGCACAGTTT GAAGTAGCCT GGGGATGAAG TTTCATATGA 961 CAAGAATGGA AATGAAAGTT TTAACTCATA AAACATGGGA TGGGAAATTA TCAAGATAAA 1021 ACAGCTCACT AATTCACAGT AATACCCTCT GAGGCTTAAT TGCAGAAATA ATAGAATAAG 1081 CCAGAGGACT GTACAGGTCC TTGGCTTTGG GGGCAAATGG TTTT // |
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| Primer: T7Terminator | RDB13961_A6Btb.seq |
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>D03144A4_A6Bt_1_T7Terminator_A04_01.ab1 1 CAATGCATCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGCTCG 61 AGTGCGGCCG CAAGCTTGTC GACGGAGCTC GAATTCGAGC TCGGTACCCT ATGAATCTAA 121 TACATGTAAA CCAAATTTAT TGTATAGTAA TGTCCAAAAC AAATCTAATA TTTACTTTAT 181 TATAACTCTA AAATATGAAA TAAAAAACTG ATTTTAGACT AGCTAAAATC AGTTTTTACT 241 TTAGTTTAAT TGTAAGTAAT ACTAGATCCA GGGTATAAAG TTGTTCCCCA TATTGAAACA 301 TTTATATTAT TTGTTAATGG AACATCATAT TCACTTATAA CATCTCTCCA CCATTCCCAA 361 GCAAGGCCTG TACACTCTCT TGCTTTTATT CTTATATTTC TTGCATTAGC TTCAAGAGGT 421 ATTACTGTTG AATAGTGAGC TGTTTTATCT TGATAATTTC CATCCCATGT TTTATGAGTT 481 AAAACTTCAT TTCCTTCTTT GTCATATGAA ACTTCATCCC AGGCTACTTC AAACTGTGCA 541 ACATAGGCTC CACTATGATC TAAGTTTATT TTTCCCTTGC TAGCCTTGTA CAGCTCGTCC 601 ATGCCGAGAG TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC 661 TCGTTGGGGT CTTTGCTCAG GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC 721 ACGGGGCCGT CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGC // |
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| Primer: pGL3_RV4 | RDB13961_A6Btc.seq |
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>D03144A4_A6Bt_1_pGL3_RV4_F12_18.ab1 1 GGGACGATGG GTTGAGGCTC TCAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC 61 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 121 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG 181 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC 241 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT 301 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC 361 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC 421 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT 481 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT 541 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT 601 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC 661 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC 721 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC 781 ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC 841 CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG 901 TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT 961 GGAGTGGCAC GCCCATCAGC AACGACTGTT TGCCGCCAGT GTTGTGCCAC GCGTTGGGAT 1021 GTAATCCAGC TCGCAATCGC CGGCTCAACT TTTTCCCGCG TTTCGCAGAA CGTTGTCTGA 1081 CTGGTCACCC ACGCGGGGAA CT // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.06.21
GNP_filter3_RDBDEP_html_190430.pl