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pET28/His6-EGFP-D4 (#RDB13961)

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens.


Drawn by SnapGene® software
Sequence (full) RDB13961hts02.seq
Publication Abe, M., Mol. Cell. Biol. 32 (8): 1396-1407 (2012). [link to RRC of NBRP]
Publication Shimada, Y., Eur. J. Biochem. 269 (24): 6195-6203 (2002). [link to RRC of NBRP]
Test sheet RDB13961_B0F4p1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 (domain 4 of theta toxin; K363-N472) of C. perfringens.
Comment Expression was confirmed by the depositor.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Km^r
Growth conditions LB+Kan, 37oC
Gene/insert name Clostridium perfringens pfoA cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimada, Y., Eur J Biochem. 269 (24): 6195-6203, 2002.)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimada, Y., Eur J Biochem. 269 (24): 6195-6203, 2002.)。

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RDB13961 pET28/His6-EGFP-D4 DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/His6-EGFP-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13961).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]
Shimada, Y., Maruya, M., Iwashita, S., Ohno-Iwashita, Y., The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains. Eur. J. Biochem. 269 (24): 6195-6203 (2002). PMID 12473115. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]
original Shimada, Y., The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains. Eur. J. Biochem. 269 (24): 6195-6203 (2002). PMID 12473115. [link to RRC of NBRP]
reference Abe, M., Imaging cholesterol depletion at the plasma membrane by methyl-β-cyclodextrin. J. Lipid Res. 62:100077 (2021). PMID 33891936.
protocol Eichler, J., Methods for Visualizing and Quantifying Cholesterol Distribution in Mammalian Cells Using Filipin and D4 Probes. Methods Mol. Biol. 2888: 101-118 (2025). PMID 39699727.
protocol Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303.
user_report Honda, A., Very-long-chain fatty acids are crucial to neuronal polarity by providing sphingolipids to lipid rafts. Cell Rep. 42 (10): 113195 (2023). PMID 37816355. [link to RRC of NBRP]
user_report Lee, J.H., 25-Hydroxycholesterol suppress IFN-γ-induced inflammation in microglia by disrupting lipid raft formation and caveolin-mediated signaling endosomes. Free Radic. Biol. Med. 179: 252-265 (2022). PMID 34808332. [link to RRC of NBRP]
user_report Woo, J.H., Interleukin-6 signaling requires EHD1-mediated alteration of membrane rafts. FEBS J. 289 (19): 5914-5932 (2022). PMID 35429212. [link to RRC of NBRP]