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pET28/His6-NT-Lys (#RDB19215)

Expression vector of His-tagged nontoxic Lysenin for E. coli.


Drawn by SnapGene® software
Sequence (full) RDB19215hts01.seq
Publication Abe, M., Cell Rep. 37 (6): 109935 (2021). [link to RRC of NBRP]
Test sheet RDB19215_B1Grp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of His-tagged nontoxic Lysenin for E. coli.
Comment Expression was confirmed by the depositor with western blotting with anti-his antibody.
Vector backbone pET-28b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Growth conditions LB+Kanamycin, 37oC
Gene/insert name Eisenia fetida Lysenin cDNA
Depositor|Developer Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19215 pET28/His6-NT-Lys DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pET28/His6-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19215).

Reference section:

Abe, M., Makino, A., Murate, M., Hullin-Matsuda, F., Yanagawa, M., Sako, Y., Kobayashi, T., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]