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pET28/His6-PMP2(I43N) (#RDB19204)

Expression vector of His-tagged human PMP2 (I43N) for E. coli.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Abe, M., Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of His-tagged human PMP2 (I43N) for E. coli. The I43N mutation results in high affinity form.
Comment Expression was confirmed by the depositor with western blotting with anti-his antibody.
Vector backbone pET-28b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human PMP2 cDNA
Depositor|Developer Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。

Catalog # Resource name Shipping form Fee
RDB19204 pET28/His6-PMP2(I43N) DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/His6-PMP2(I43N) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19204).

Reference section:

Abe, M., Makino, A., Murate, M., Hullin-Matsuda, F., Yanagawa, M., Sako, Y., Kobayashi, T., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [PubMed] [Article] [RRC of NBRP]