Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pET28/His6-PMP2(I43N) 

Expression vector of His-tagged human PMP2 (I43N) for E. coli.

Catalog number RDB19204
Resource name pET28/His6-PMP2(I43N) 
Clone info. Expression vector of His-tagged human PMP2 (I43N) for E. coli. The I43N mutation results in high affinity form.
Comment Expression was confirmed by the depositor with western blotting with anti-his antibody.
Vector backbone pET-28b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Gene/insert name human PMP2 cDNA
Depositor|Developer Abe, Mitsuhiro |
 
Sequence (full) RDB19204hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19204_B1Grp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
提供案内 (日本国内) [open/close]

提供依頼書
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19204 pET28/His6-PMP2(I43N)  DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/His6-PMP2(I43N)  was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19204).

Reference section:

Abe, M., Makino, A., Murate, M., Hullin-Matsuda, F., Yanagawa, M., Sako, Y., Kobayashi, T., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]