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pET28/His6-EGFP-Lact-C2 

Expression vector of His and EGFP-tagged C2 domain of bovine Lactadherin for E. coli. Phosphatidylserine (PS)-specific probe.

Catalog number RDB19214
Resource name pET28/His6-EGFP-Lact-C2 
Clone info. Expression vector of His and EGFP-tagged C2 domain of bovine Lactadherin for E. coli.
Comment Expression was confirmed by the depositor with western blotting with anti-his antibody.
Vector backbone pET-28b (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Gene/insert name Bos taurus MFGE8 cDNA
Depositor|Developer Abe, Mitsuhiro |
Other clones in our bank
External Database
Bos taurus MFGE8
          Reference sequence
            
           
          Sequence (full) RDB19214hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19214_B1Grp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19214 pET28/His6-EGFP-Lact-C2  DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pET28/His6-EGFP-Lact-C2  was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19214).

          Reference section:

          Abe, M., Makino, A., Murate, M., Hullin-Matsuda, F., Yanagawa, M., Sako, Y., Kobayashi, T., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [link to RRC of NBRP]

          2023.09.06

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