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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-CeRhoA (#RDB16767)

Expression vector of C. elegans RhoA.

Clone info. Expression vector of C. elegans RhoA fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from C. elegans cDNA library by PCR amplification.Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name C. elegans RhoA cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012）の引用を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16767 pDEST/mCherry-CeRhoA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-CeRhoA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16767).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16767_A8Kmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Red-C_F ()
Sequence file: RDB16767_A8Kma.seq check
>D05982A4_A8Km_2_Red-C_F_G09_21_ABI24.ab1
    1 CAGTCAATCA AGTTGGACTC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG
   61 AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG AAGCTTATGG
  121 CTGCGATTAG AAAGAAGTTG GTGATCGTCG GCGACGGAGC CTGCGGTAAA ACTTGCCTGC
  181 TCATCGTTTT CTCAAAGGAT CAGTTCCCAG ACGTGTATGT GCCGACTGTT TTCGAGAATT
  241 ATGTTGCCGA CATTGAAGTT GACGGAAAGC AGGTCGAACT TGCTCTATGG GATACAGCTG
  301 GACAAGAGGA CTATGATCGT CTGCGTCCAC TCTCTTATCC GGATACTGAC GTCATTCTGA
  361 TGTGCTTCTC AATTGATTCA CCCGATTCAC TGGAGAATAT TCCAGAAAAA TGGACACCAG
  421 AAGTCAGGCA TTTCTGTCCA AATGTTCCGA TTATTTTGGT CGGAAATAAA CGAGATCTTC
  481 GTAGTGATCC ACAGACTGTT CGAGAATTGG CAAAAATGAA ACAGGAGCCC GTGAAGCCTG
  541 AGCAGGGACG AGCAATTGCT GAGCAAATTG GAGCATTTGC ATATTTGGAG TGCTCTGCGA
  601 AGACTAAGGA CGGAATTCGT GAGGTATTCG AGAAGGCGAC ACAGGCGGCA TTGCAACAGA
  661 AGAAGAAAAA GAAGAGCAAG TGCATGATTT TGTAACTCGA GGTTTAAACC CGCTGATCAG
  721 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT
  781 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC
  841 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG
  901 GAGGATTGGG AAGACAATAG CAGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG
  961 CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA
 1021 GCGCGGCGGG TGTGGTGGTT TACGCGCAGC GTGACCGCTA CACTTTGCCA GCGCCTAGCG
 1081 CCCGCCTCTT TCGCTTTCTT CCATTCGTTC TCGCCACGTT CGCAGCTTTC CCCGATCAAG
1141 CTCTTAAAAT TCG
//
Primer: CMV_Forward ()
Sequence file: RDB16767_A8Kmb.seq check
>D05982A4_A8Km_2_CMV_Forward_E09_15_ABI24.ab1
    1 TGGTACACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA
  181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
  361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
  481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
  541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
  601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
  661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
  721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
  781 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
  841 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGAAG CTTATGGCTG CGATTAGAAA
  901 GAAGTTGGTG ATCGTCGGCG ACGGAGCCTG CGGTAAAACT TGCCTGCTCA TCGTTTTCTC
  961 AAAGGATCAG TTCCCAGACG TGTATGTGCC GACTGTTTTC GAGAATTATG TTGCCGACAT
 1021 TGAAAGTTGA CGGGAAAGCA GGTCGAAACT TTGCTCTATG GGATACAGCT GGGACAAGAG
 1081 GACTATGATC GTCTGCGTCC AACTCCTCTT AATCGCATAC TGAACGGTTC AATCCTGAAT
1141 GTGCATTCCC TCCAACTTGG AATTTCGAC
//
Primer: SV40pro_F ()
Sequence file: RDB16767_A8Kmc.seq check
>D05982A4_A8Km_2_SV40pro_F_H09_24_ABI24.ab1
    1 CCTGTCCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGCT
 1021 TCGGAATCGT TTTCCCGGGG ACGCCGGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
 1081 TGGAGTTCTT TCGCCCACCC CGACCTGGTT AATGGCCAGC CTATAATTGA TTACGAATAA
1141 AAGCAATAGC CAATCCACCG AAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]