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pDEST/mCherry-CeRhoA

Expression vector of C. elegans RhoA.

Catalog number RDB16767
Resource name pDEST/mCherry-CeRhoA
Clone info. Expression vector of C. elegans RhoA fused with mCherry at N-terminus.
Expression was confirmed by the depositor with immunofluorescence. Insert gene, from C. elegans cDNA library by PCR amplification.Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name C. elegans RhoA cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |
 
Other clones in our bank C. elegans rho-1 (KEGG orthology K04513) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012) is requested.
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RDB16767 pDEST/mCherry-CeRhoA DNA solution

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References and tips

Electronic file

Featured content

References

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16767_A8Kmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Red-C_F RDB16767_A8Kma.seq
>D05982A4_A8Km_2_Red-C_F_G09_21_ABI24.ab1
    1 CAGTCAATCA AGTTGGACTC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG
   61 AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG AAGCTTATGG
  121 CTGCGATTAG AAAGAAGTTG GTGATCGTCG GCGACGGAGC CTGCGGTAAA ACTTGCCTGC
  181 TCATCGTTTT CTCAAAGGAT CAGTTCCCAG ACGTGTATGT GCCGACTGTT TTCGAGAATT
  241 ATGTTGCCGA CATTGAAGTT GACGGAAAGC AGGTCGAACT TGCTCTATGG GATACAGCTG
  301 GACAAGAGGA CTATGATCGT CTGCGTCCAC TCTCTTATCC GGATACTGAC GTCATTCTGA
  361 TGTGCTTCTC AATTGATTCA CCCGATTCAC TGGAGAATAT TCCAGAAAAA TGGACACCAG
  421 AAGTCAGGCA TTTCTGTCCA AATGTTCCGA TTATTTTGGT CGGAAATAAA CGAGATCTTC
  481 GTAGTGATCC ACAGACTGTT CGAGAATTGG CAAAAATGAA ACAGGAGCCC GTGAAGCCTG
  541 AGCAGGGACG AGCAATTGCT GAGCAAATTG GAGCATTTGC ATATTTGGAG TGCTCTGCGA
  601 AGACTAAGGA CGGAATTCGT GAGGTATTCG AGAAGGCGAC ACAGGCGGCA TTGCAACAGA
  661 AGAAGAAAAA GAAGAGCAAG TGCATGATTT TGTAACTCGA GGTTTAAACC CGCTGATCAG
  721 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT
  781 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC
  841 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG
  901 GAGGATTGGG AAGACAATAG CAGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG
  961 CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA
 1021 GCGCGGCGGG TGTGGTGGTT TACGCGCAGC GTGACCGCTA CACTTTGCCA GCGCCTAGCG
 1081 CCCGCCTCTT TCGCTTTCTT CCATTCGTTC TCGCCACGTT CGCAGCTTTC CCCGATCAAG
1141 CTCTTAAAAT TCG
//
Primer: CMV_Forward RDB16767_A8Kmb.seq
>D05982A4_A8Km_2_CMV_Forward_E09_15_ABI24.ab1
    1 TGGTACACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA
  181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
  361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
  481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
  541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
  601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
  661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
  721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
  781 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
  841 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGAAG CTTATGGCTG CGATTAGAAA
  901 GAAGTTGGTG ATCGTCGGCG ACGGAGCCTG CGGTAAAACT TGCCTGCTCA TCGTTTTCTC
  961 AAAGGATCAG TTCCCAGACG TGTATGTGCC GACTGTTTTC GAGAATTATG TTGCCGACAT
 1021 TGAAAGTTGA CGGGAAAGCA GGTCGAAACT TTGCTCTATG GGATACAGCT GGGACAAGAG
 1081 GACTATGATC GTCTGCGTCC AACTCCTCTT AATCGCATAC TGAACGGTTC AATCCTGAAT
1141 GTGCATTCCC TCCAACTTGG AATTTCGAC
//
Primer: SV40pro_F RDB16767_A8Kmc.seq
>D05982A4_A8Km_2_SV40pro_F_H09_24_ABI24.ab1
    1 CCTGTCCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGCT
 1021 TCGGAATCGT TTTCCCGGGG ACGCCGGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
 1081 TGGAGTTCTT TCGCCCACCC CGACCTGGTT AATGGCCAGC CTATAATTGA TTACGAATAA
1141 AAGCAATAGC CAATCCACCG AAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl