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| Catalog # | Name of clone | Running title | Publication |
| RDB16767 | pDEST/mCherry-CeRhoA | Expression vector of C. elegans RhoA. | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB16766 | pDEST/mCherry-TubbyC | Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C). | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB16765 | pDEST/mCherry-PIP5KB | Expression vector of human phosphatidylinositol 4-phosphate 5-kinase beta (PIP5K beta). | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB16764 | pDEST/mCherry-PLCD1-PH | Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1). | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB15996 | EGFR-EGFP | Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP. | Hiroshima, M., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14701 | pDEST/mCherry-Rab18 S22N | Expression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14700 | pDEST/mCherry-Rab18 Q67L | Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14699 | pDEST/mCherry-Rab18 | Expression vector of human Rab18, wild type, fused with mCherry. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14698 | pSpCas9(BB)-2A-puro-ACAT2 sgRNA | Expression vector of sgRNA for targeting human ACAT2 and Cas9. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14697 | pSpCas9(BB)-2A-puro-ACAT1 sgRNA | Expression vector of sgRNA for targeting human ACAT1 and Cas9. | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14301 | pET28/His6-EGFP-Nakanori | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. | Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14300 | pET28/His6-mCherry-D4 | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens. | Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14156 | pCMV-GFP-ERK2 | Expression vector of GFP-tagged human ERK2. | Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14154 | pACYC184-Venus-MEK1 (S218/222E, delta32-51) | Plasmid clone of constitutive active form of human MEK1. | Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB14152 | pGEX-GFP-ERK2-His | Bacterial expression vector of GFP–human ERK2. | Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB13257 | GL107 | Expression plasmid of GFP-tagged human cRaf1 (R89A mutant). | Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB13256 | GL207 | Expression plasmid of GFP-tagged human cRaf1 CRDCAD (R89A S621A). | Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB13255 | GL348 | Expression plasmid of GFP-tagged human cRaf1 catalytic domain (CAD, 330-627) | Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [PubMed] [Article] [RRC of NBRP] (original) |
| RDB13254 | GL023 | Expression plasmid of GFP-tagged human c-RAF. | Hibino K, A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition. Biophys. J. 97 (5): 1277-1287 (2009). PMID 19720015. [PubMed] [Article] [RRC of NBRP] (original) |
♦ No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
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2025.04.14
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