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GL107

Expression plasmid of GFP-tagged human cRaf1 (R89A mutant).

Catalog number RDB13257
Resource name GL107
Clone info. Expression plasmid of human cRaf1 (R89A). This clone contains full cds of NM_002880.3. The R89A point mutation is reported to disrupt the Ras-binding activity of Ras-binding domain (RDB). Very little R89A mutant RAF was observed at the plasma membrane. See Fig.7 of Hibino, K. Biophys. J. 97 (5), 1277-1287, 2009.
Vector backbone pmEGFP-C2-NheI (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human RAF1 cDNA
Depositor Sako, Yasushi |
 
Other clones in our bank human RAF1 (NCBI Gene 5894) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13257 GL107 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAP kinases (English text)

References

original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13257_A5D8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13257_A5D8a.seq
>13257_13257_A5D8_3_CMV-Forward_B11_05.ab1
    1 TAAAAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGATC TCGATAGCGC TACCGGTCGC
  121 CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
  181 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
  241 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
  301 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
  361 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
  421 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
  481 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
  541 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA
  601 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT
  661 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
  721 CCACTACCTG AGCACCCAGT CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT
  781 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
  841 GTCCGGCCGG ACTCAGATCT CGAGCTCAAG CTTCGAATTC ATGGAGCACA TACAGGGAGC
  901 TTGGAAGACG ATCAGCAATG GTTTTGGATT CAAAGATGCC GTGTTTGATG GCTCCAGCTG
  961 CATCTCTCCT ACAATAGTTC AGCAGTTTGG CTATCAGCGC CCGGGCATCA GATGATGGCA
 1021 AACTCACAGA TCCCTTTCTT AGGACAGCAA CACTATCCCG TGTTTTCATG GCGGACAGCA
 1081 AGACAGTGAT CATGTGCGAA TGGATTGAGC TGCAATGACT GCCTTATTGA AAGGCCAACC
1141 TTCTAG
//
Primer: EGFP-C RDB13257_A5D8b.seq
>13257_13257_A5D8_3_EGFP-C_C11_08.ab1
    1 CGGTACTCTC GGCATGGACG AGCTGTACAA GTCCGGCCGG ACTCAGATCT CGAGCTCAAG
   61 CTTCGAATTC ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTTGGATT
  121 CAAAGATGCC GTGTTTGATG GCTCCAGCTG CATCTCTCCT ACAATAGTTC AGCAGTTTGG
  181 CTATCAGCGC CGGGCATCAG ATGATGGCAA ACTCACAGAT CCTTCTAAGA CAAGCAACAC
  241 TATCCGTGTT TTCTTGCCGA ACAAGCAAAG AACAGTGGTC AATGTGCGAA ATGGAATGAG
  301 CTTGCATGAC TGCCTTATGA AAGCACTCAA GGTGGCGGGC CTGCAACCAG AGTGCTGTGC
  361 AGTGTTCAGA CTTCTCCACG AACACAAAGG TAAAAAAGCA CGCTTAGATT GGAATACTGA
  421 TGCTGCGTCT TTGATTGGAG AAGAACTTCA AGTAGATTTC CTGGATCATG TTCCCCTCAC
  481 AACACACAAC TTTGCTCGGA AGACGTTCCT GAAGCTTGCC TTCTGTGACA TCTGTCAGAA
  541 ATTCCTGCTC AATGGATTTC GATGTCAGAC TTGTGGCTAC AAATTTCATG AGCACTGTAG
  601 CACCAAAGTA CCTACTATGT GTGTGGACTG GAGTAACATC AGACAACTCT TATTGTTTCC
  661 AAATTCCACT ATTGGTGATA GTGGAGTCCC AGCACTACCT TCTTTGACTA TGCGTCGTAT
  721 GCGAGAGTCT GTTTCCAGGA TGCCTGTTAG TTCTCAGCAC AGATATTCTA CACCTCACGC
  781 CTTCACCTTT AACACCTCCA GTCCCTCATC TGAAGGTTCC CTCTCCCAGA GGCAGAGGTC
  841 GACATCCACA CCTAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT
  901 TGAGGATGCA ATTCGAAGTC ACAGCGAATC GGCCTCACCT TCAGCCCTGT CCAGTAGCCC
  961 CAACAATCTG AGCCCAACAG GCTGGGTCAC AGCCGAAAAC CCCCGTGCCA GCACAAAGAG
 1021 AGCGGGCACC AGTATCTGGG ACCCAGGAGA AAAACAAAAT TAGGCCTCGT GGACAGAAGA
 1081 GATCCAGCCT ATATGGGAAA TAGAGCCAGT GAAGTGATGC TGTCCACTCG ATGGGTCAGC
1141 TCTTTTTGGA ACGGCTA
//
Primer: pAxEF_R2 RDB13257_A5D8c.seq
>13257_13257_A5D8_3_pAxEF_R2_D05_11.ab1
    1 AGGAAAAAAT GCCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCAGTTAT CTAGATCCGG TGGATCCCTA GAAGACAGGC AGCCTCGGGG ACGTGGTCAG
  241 CGTGCAAGCA TTGATATCCT CAGTGTGGGC TGCCCGATGC AAGGATGGCT CGGAAGCGCT
  301 CCGGTTGATC TTCGGTAGAG AGTGTTGGAG CAGCTCAATG GAAGACAGGA TCTGGGGAAA
  361 AAGAGGCCTC TCTTCCTTTA CTTTCTTCAC ACAGTCAGCT ACCAGCCTCT TCATTGCTTT
  421 GGGGCAGTTC TTATATAGCT TACTAAGATC TGGGGAGGCA TATCCTCGGC CCACCATGAA
  481 GATGATCTGA TCTCGGTTGT TGATGTGAGA ATAAGGAAGC TCCCCCGTCA TCAGTTCATA
  541 CAATACGATG CCATAGGAGT AGACATCCGA CTGGAAACTG AATGGGTTGT TATCCTGCAT
  601 TCGGATCACC TCTGGGGCCA TCCAGAGGAC AGAGCCAGTA GGTTGTTCAA CCTGCTGAGA
  661 ACCACTCCAG CGTGACTTTA CTGTTGCCAA ACCAAAATCT CCAATTTTCA CTGTTAAGCC
  721 TTCATGGAGA AATATATTGT TGGATTTCAT GTCTCTATGG ATGATGTTCT TTGCATGCAA
  781 ATAGTCCATT CCCTGAGCCG TCTGCCGGGC AATGTCAATT AGCTGGAACA TCTGAAACTT
  841 GGTCTCCTGG ACATGCAGGT GTTTGTAGAG GCTGCTGCCC TCGCACCACT GGGTCACAAT
  901 TGCCAGGTTG TCCTTTGTCA TGTACCCCAT GAAAAGCAGA ATGTTCACAT GCCGTGTTTT
  961 GCGCAGAACA GCCACCTCAT TCCTGAACGG CCTGGAAATT GCTCTGGGGG TTGGGTCGAC
 1021 AACCCTTTAG GATCTTTACT GCAACATCTC CGTGCCCATT TACCCTTAAT AAACAGCTCC
 1081 AAAAGAGCCC TGACCCAATC GAAGTGACAG CATTCACGCT CACTTGACCT TCCTATTTTC
1141 CCAAATAAAT AAGCCGTG
//
Primer: SV40pro_F_V2 RDB13257_A5D8d.seq
>13257_13257_A5D8_3_SV40proF_V2_E11_14.ab1
    1 TGAGGGTTTT TTTGGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
  901 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
  961 CGGAATCGTT TTCCGGGACG CCCGGCTGGA TGATCCTTCC AGCGCGGGGA TCTCATGCTG
 1021 AGTTCTTCGC CACCCTAGGG GAGGCTAACT GAACACGGGA GGAGACAATA CCGGAAGGAA
 1081 CCCGCGCTTA TGACGGCATA AAAGACGATA AACGCACGGT GATGGTTCGT TTTGTTCATA
1141 AACGCCGGGG GTTTCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl