Please review the QC test results indicated by icon below as well as clone information before placing your order.

GL348

Expression plasmid of GFP-tagged human cRaf1 catalytic domain (CAD, 330-627)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13255_A5D8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13255_A5D8a.seq
>13255_13255_A5D8_1_CMV-Forward_B10_04.ab1     1 TTCCAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG   121 ACCATGATTA GGCCTCGTGG ACAGAGAGAT TCAAGCTATT ATTGGGAAAT AGAAGCCAGT   181 GAAGTGATGC TGTCCACTCG GATTGGGTCA GGCTCTTTTG GAACTGTTTA TAAGGGTAAA   241 TGGCACGGAG ATGTTGCAGT AAAGATCCTA AAGGTTGTCG ACCCAACCCC AGAGCAATTC   301 CAGGCCTTCA GGAATGAGGT GGCTGTTCTG CGCAAAACAC GGCATGTGAA CATTCTGCTT   361 TTCATGGGGT ACATGACAAA GGACAACCTG GCAATTGTGA CCCAGTGGTG CGAGGGCAGC   421 AGCCTCTACA AACACCTGCA TGTCCAGGAG ACCAAGTTTC AGATGTTCCA GCTAATTGAC   481 ATTGCCCGGC AGACGGCTCA GGGAATGGAC TATTTGCATG CAAAGAACAT CATCCATAGA   541 GACATGAAAT CCAACAATAT ATTTCTCCAT GAAGGCTTAA CAGTGAAAAT TGGAGATTTT   601 GGTTTGGCAA CAGTAAAGTC ACGCTGGAGT GGTTCTCAGC AGGTTGAACA ACCTACTGGC   661 TCTGTCCTCT GGATGGCCCC AGAGGTGATC CGAATGCAGG ATAACAACCC ATTCAGTTTC   721 CAGTCGGATG TCTACTCCTA TGGCATCGTA TTGTATGAAC TGATGACGGG GGAGCTTCCT   781 TATTCTCACA TCAACAACCG AGATCAGATC ATCTTCATGG TGGGCCGAGG ATATGCCTCC   841 CCAGATCTTA GTAAGCTATA TAAGAACTGC CCCAAAGCAA TGAAGAGGCT GGTAGCTGAC   901 TGTGTGAAGA AAGTAAAGGA AGAGAGGCCT CTTTTTCCCC AGATCCTGTC TTCCATTGAG   961 CTGCTCCAAC ACTCTCTACC GAAGATCAAC CGGGAGCGCT TCCGAGCCAT CCCTTTGCAT  1021 CGGGCGAATT CTGCAGTCGA CGGTACGCGG GCCCGGGATC CACCCGGGTC GCAACAATGG  1081 TTGAGCAGGC GAGAGCTGAT CAACGGGTTG TTGCCATCCC TGGTCGAGGC TTGGGACCGG 1141 GCCGAA //
Primer: pAxEF_R2 Sequence file: RDB13255_A5D8b.seq
>13255_13255_A5D8_1_pAxEF_R2_B05_05.ab1     1 CGTCAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC    61 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG   121 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT   181 CTAGAGTCGC GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG   241 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG   301 GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG   361 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG   421 AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG   481 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC   541 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG   601 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC   661 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG   721 GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC   781 TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG   841 TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC   901 TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC   961 AGAATTCGCC GATGCAAGGA TGGCTCGGAA GCGCTCCGGT TGATCTTTCG GTAGAGAGTG  1021 TTTGGAGCAG CTCAATGGAA GACAGGATCT GGGGGAAAAA GAGGCCTCTC TTCGTTTACT  1081 TTCTTCACAC AGTCAGCTAA CAAGCTTCAT CCATGCTTGC GTCAGTCCTT ATATAAGCTA 1141 CTACGATCTG AGTGCAAGCC ATATATCCCC TG //
Primer: SV40pro_F_V2 Sequence file: RDB13255_A5D8c.seq
>13255_13255_A5D8_1_SV40proF_V2_E10_13.ab1     1 GAAGAACTTT TTTGGAGGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC    61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG   901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC   961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG  1021 TTCTTCGCCC ACCCTTAGGG GGGAGCTAAC TGAAACACGG AAGGAGACAT ACGGAAGACC  1081 CGCGCTATGA CGCATAAAGA CGATAAACGC CACGGTGTTG GGTCGGTTTT TGTTTCACAT 1141 TAAAAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl