Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
GL348 (#RDB13255)
Expression plasmid of GFP-tagged human cRaf1 catalytic domain (CAD, 330-627)
| Clone info. | Expression plasmid of human cRaf1 catalytic domain (CAD, 330-627), CAD-GFP in Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. |
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| Comment | monomeric EGFP. |
| Vector backbone | pmEGFP-N1 (Plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r |
| Gene/insert name | Human RAF1 cDNA |
| Depositor|Developer | Sako, Yasushi | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011) |
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[open/close]| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 286 (42): 36460-36468, 2011)。 |
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| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB13255 | GL348 | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The GL348 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13255). |
Reference section:
| Hibino, K., Shibata, T., Yanagida, T., Sako, Y., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13255_A5D8.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB13255_A5D8a.seq
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>13255_13255_A5D8_1_CMV-Forward_B10_04.ab1 1 TTCCAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG 121 ACCATGATTA GGCCTCGTGG ACAGAGAGAT TCAAGCTATT ATTGGGAAAT AGAAGCCAGT 181 GAAGTGATGC TGTCCACTCG GATTGGGTCA GGCTCTTTTG GAACTGTTTA TAAGGGTAAA 241 TGGCACGGAG ATGTTGCAGT AAAGATCCTA AAGGTTGTCG ACCCAACCCC AGAGCAATTC 301 CAGGCCTTCA GGAATGAGGT GGCTGTTCTG CGCAAAACAC GGCATGTGAA CATTCTGCTT 361 TTCATGGGGT ACATGACAAA GGACAACCTG GCAATTGTGA CCCAGTGGTG CGAGGGCAGC 421 AGCCTCTACA AACACCTGCA TGTCCAGGAG ACCAAGTTTC AGATGTTCCA GCTAATTGAC 481 ATTGCCCGGC AGACGGCTCA GGGAATGGAC TATTTGCATG CAAAGAACAT CATCCATAGA 541 GACATGAAAT CCAACAATAT ATTTCTCCAT GAAGGCTTAA CAGTGAAAAT TGGAGATTTT 601 GGTTTGGCAA CAGTAAAGTC ACGCTGGAGT GGTTCTCAGC AGGTTGAACA ACCTACTGGC 661 TCTGTCCTCT GGATGGCCCC AGAGGTGATC CGAATGCAGG ATAACAACCC ATTCAGTTTC 721 CAGTCGGATG TCTACTCCTA TGGCATCGTA TTGTATGAAC TGATGACGGG GGAGCTTCCT 781 TATTCTCACA TCAACAACCG AGATCAGATC ATCTTCATGG TGGGCCGAGG ATATGCCTCC 841 CCAGATCTTA GTAAGCTATA TAAGAACTGC CCCAAAGCAA TGAAGAGGCT GGTAGCTGAC 901 TGTGTGAAGA AAGTAAAGGA AGAGAGGCCT CTTTTTCCCC AGATCCTGTC TTCCATTGAG 961 CTGCTCCAAC ACTCTCTACC GAAGATCAAC CGGGAGCGCT TCCGAGCCAT CCCTTTGCAT 1021 CGGGCGAATT CTGCAGTCGA CGGTACGCGG GCCCGGGATC CACCCGGGTC GCAACAATGG 1081 TTGAGCAGGC GAGAGCTGAT CAACGGGTTG TTGCCATCCC TGGTCGAGGC TTGGGACCGG 1141 GCCGAA // |
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Primer: pAxEF_R2 Sequence file: RDB13255_A5D8b.seq
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>13255_13255_A5D8_1_pAxEF_R2_B05_05.ab1 1 CGTCAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC 61 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG 121 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT 181 CTAGAGTCGC GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG 241 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG 301 GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG 361 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG 421 AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG 481 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC 541 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG 601 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC 661 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG 721 GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC 781 TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG 841 TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC 901 TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC 961 AGAATTCGCC GATGCAAGGA TGGCTCGGAA GCGCTCCGGT TGATCTTTCG GTAGAGAGTG 1021 TTTGGAGCAG CTCAATGGAA GACAGGATCT GGGGGAAAAA GAGGCCTCTC TTCGTTTACT 1081 TTCTTCACAC AGTCAGCTAA CAAGCTTCAT CCATGCTTGC GTCAGTCCTT ATATAAGCTA 1141 CTACGATCTG AGTGCAAGCC ATATATCCCC TG // |
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Primer: SV40pro_F_V2 Sequence file: RDB13255_A5D8c.seq
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>13255_13255_A5D8_1_SV40proF_V2_E10_13.ab1 1 GAAGAACTTT TTTGGAGGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC 961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG 1021 TTCTTCGCCC ACCCTTAGGG GGGAGCTAAC TGAAACACGG AAGGAGACAT ACGGAAGACC 1081 CGCGCTATGA CGCATAAAGA CGATAAACGC CACGGTGTTG GGTCGGTTTT TGTTTCACAT 1141 TAAAAC // |
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Sequencing and PCR primers for primer information.
References
Original, user report and related articles
| original | Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [link to RRC of NBRP] |
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