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GL348

Expression plasmid of GFP-tagged human cRaf1 catalytic domain (CAD, 330-627)

Catalog number RDB13255
Resource name GL348
Clone info. Expression plasmid of human cRaf1 catalytic domain (CAD, 330-627), CAD-GFP in Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.
Comment monomeric EGFP.
Vector backbone pmEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human RAF1 cDNA
Depositor|Developer Sako, Yasushi |
Other clones in our bank

External Database
Human RAF1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 286 (42): 36460-36468, 2011)。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13255 GL348 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content MAP kinases (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13255_A5D8.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13255_A5D8a.seq check
          >13255_13255_A5D8_1_CMV-Forward_B10_04.ab1
              1 TTCCAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG
            121 ACCATGATTA GGCCTCGTGG ACAGAGAGAT TCAAGCTATT ATTGGGAAAT AGAAGCCAGT
            181 GAAGTGATGC TGTCCACTCG GATTGGGTCA GGCTCTTTTG GAACTGTTTA TAAGGGTAAA
            241 TGGCACGGAG ATGTTGCAGT AAAGATCCTA AAGGTTGTCG ACCCAACCCC AGAGCAATTC
            301 CAGGCCTTCA GGAATGAGGT GGCTGTTCTG CGCAAAACAC GGCATGTGAA CATTCTGCTT
            361 TTCATGGGGT ACATGACAAA GGACAACCTG GCAATTGTGA CCCAGTGGTG CGAGGGCAGC
            421 AGCCTCTACA AACACCTGCA TGTCCAGGAG ACCAAGTTTC AGATGTTCCA GCTAATTGAC
            481 ATTGCCCGGC AGACGGCTCA GGGAATGGAC TATTTGCATG CAAAGAACAT CATCCATAGA
            541 GACATGAAAT CCAACAATAT ATTTCTCCAT GAAGGCTTAA CAGTGAAAAT TGGAGATTTT
            601 GGTTTGGCAA CAGTAAAGTC ACGCTGGAGT GGTTCTCAGC AGGTTGAACA ACCTACTGGC
            661 TCTGTCCTCT GGATGGCCCC AGAGGTGATC CGAATGCAGG ATAACAACCC ATTCAGTTTC
            721 CAGTCGGATG TCTACTCCTA TGGCATCGTA TTGTATGAAC TGATGACGGG GGAGCTTCCT
            781 TATTCTCACA TCAACAACCG AGATCAGATC ATCTTCATGG TGGGCCGAGG ATATGCCTCC
            841 CCAGATCTTA GTAAGCTATA TAAGAACTGC CCCAAAGCAA TGAAGAGGCT GGTAGCTGAC
            901 TGTGTGAAGA AAGTAAAGGA AGAGAGGCCT CTTTTTCCCC AGATCCTGTC TTCCATTGAG
            961 CTGCTCCAAC ACTCTCTACC GAAGATCAAC CGGGAGCGCT TCCGAGCCAT CCCTTTGCAT
           1021 CGGGCGAATT CTGCAGTCGA CGGTACGCGG GCCCGGGATC CACCCGGGTC GCAACAATGG
           1081 TTGAGCAGGC GAGAGCTGAT CAACGGGTTG TTGCCATCCC TGGTCGAGGC TTGGGACCGG
          1141 GCCGAA
          //
          Primer: pAxEF_R2
          Sequence file: RDB13255_A5D8b.seq check
          >13255_13255_A5D8_1_pAxEF_R2_B05_05.ab1
              1 CGTCAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
             61 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
            121 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
            181 CTAGAGTCGC GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG
            241 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG
            301 GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG
            361 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG
            421 AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG
            481 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC
            541 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG
            601 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC
            661 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG
            721 GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC
            781 TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG
            841 TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC
            901 TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
            961 AGAATTCGCC GATGCAAGGA TGGCTCGGAA GCGCTCCGGT TGATCTTTCG GTAGAGAGTG
           1021 TTTGGAGCAG CTCAATGGAA GACAGGATCT GGGGGAAAAA GAGGCCTCTC TTCGTTTACT
           1081 TTCTTCACAC AGTCAGCTAA CAAGCTTCAT CCATGCTTGC GTCAGTCCTT ATATAAGCTA
          1141 CTACGATCTG AGTGCAAGCC ATATATCCCC TG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13255_A5D8c.seq check
          >13255_13255_A5D8_1_SV40proF_V2_E10_13.ab1
              1 GAAGAACTTT TTTGGAGGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
            901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
            961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
           1021 TTCTTCGCCC ACCCTTAGGG GGGAGCTAAC TGAAACACGG AAGGAGACAT ACGGAAGACC
           1081 CGCGCTATGA CGCATAAAGA CGATAAACGC CACGGTGTTG GGTCGGTTTT TGTTTCACAT
          1141 TAAAAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl