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GL348

Expression plasmid of GFP-tagged human cRaf1 catalytic domain (CAD, 330-627)

Catalog number RDB13255
Resource name GL348
Clone info. Expression plasmid of human cRaf1 catalytic domain (CAD, 330-627), CAD-GFP in Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.
monomeric EGFP.
Vector backbone pmEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human RAF1 cDNA
Depositor Sako, Yasushi |
 
Other clones in our bank human RAF1 (NCBI Gene 5894) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13255 GL348 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAP kinases (English text)

References

original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13255_A5D8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13255_A5D8a.seq
>13255_13255_A5D8_1_CMV-Forward_B10_04.ab1
    1 TTCCAACTCC GGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG
  121 ACCATGATTA GGCCTCGTGG ACAGAGAGAT TCAAGCTATT ATTGGGAAAT AGAAGCCAGT
  181 GAAGTGATGC TGTCCACTCG GATTGGGTCA GGCTCTTTTG GAACTGTTTA TAAGGGTAAA
  241 TGGCACGGAG ATGTTGCAGT AAAGATCCTA AAGGTTGTCG ACCCAACCCC AGAGCAATTC
  301 CAGGCCTTCA GGAATGAGGT GGCTGTTCTG CGCAAAACAC GGCATGTGAA CATTCTGCTT
  361 TTCATGGGGT ACATGACAAA GGACAACCTG GCAATTGTGA CCCAGTGGTG CGAGGGCAGC
  421 AGCCTCTACA AACACCTGCA TGTCCAGGAG ACCAAGTTTC AGATGTTCCA GCTAATTGAC
  481 ATTGCCCGGC AGACGGCTCA GGGAATGGAC TATTTGCATG CAAAGAACAT CATCCATAGA
  541 GACATGAAAT CCAACAATAT ATTTCTCCAT GAAGGCTTAA CAGTGAAAAT TGGAGATTTT
  601 GGTTTGGCAA CAGTAAAGTC ACGCTGGAGT GGTTCTCAGC AGGTTGAACA ACCTACTGGC
  661 TCTGTCCTCT GGATGGCCCC AGAGGTGATC CGAATGCAGG ATAACAACCC ATTCAGTTTC
  721 CAGTCGGATG TCTACTCCTA TGGCATCGTA TTGTATGAAC TGATGACGGG GGAGCTTCCT
  781 TATTCTCACA TCAACAACCG AGATCAGATC ATCTTCATGG TGGGCCGAGG ATATGCCTCC
  841 CCAGATCTTA GTAAGCTATA TAAGAACTGC CCCAAAGCAA TGAAGAGGCT GGTAGCTGAC
  901 TGTGTGAAGA AAGTAAAGGA AGAGAGGCCT CTTTTTCCCC AGATCCTGTC TTCCATTGAG
  961 CTGCTCCAAC ACTCTCTACC GAAGATCAAC CGGGAGCGCT TCCGAGCCAT CCCTTTGCAT
 1021 CGGGCGAATT CTGCAGTCGA CGGTACGCGG GCCCGGGATC CACCCGGGTC GCAACAATGG
 1081 TTGAGCAGGC GAGAGCTGAT CAACGGGTTG TTGCCATCCC TGGTCGAGGC TTGGGACCGG
1141 GCCGAA
//
Primer: pAxEF_R2 RDB13255_A5D8b.seq
>13255_13255_A5D8_1_pAxEF_R2_B05_05.ab1
    1 CGTCAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC
   61 TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG
  121 GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC TGATTATGAT
  181 CTAGAGTCGC GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG
  241 GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGCTTG
  301 GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG
  361 TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG
  421 AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG
  481 TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC
  541 AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG
  601 TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC
  661 TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG
  721 GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC
  781 TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAACTTG
  841 TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT GAACAGCTCC
  901 TCGCCCTTGC TCACCATGGT GGCGACCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTGC
  961 AGAATTCGCC GATGCAAGGA TGGCTCGGAA GCGCTCCGGT TGATCTTTCG GTAGAGAGTG
 1021 TTTGGAGCAG CTCAATGGAA GACAGGATCT GGGGGAAAAA GAGGCCTCTC TTCGTTTACT
 1081 TTCTTCACAC AGTCAGCTAA CAAGCTTCAT CCATGCTTGC GTCAGTCCTT ATATAAGCTA
1141 CTACGATCTG AGTGCAAGCC ATATATCCCC TG
//
Primer: SV40pro_F_V2 RDB13255_A5D8c.seq
>13255_13255_A5D8_1_SV40proF_V2_E10_13.ab1
    1 GAAGAACTTT TTTGGAGGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
 1021 TTCTTCGCCC ACCCTTAGGG GGGAGCTAAC TGAAACACGG AAGGAGACAT ACGGAAGACC
 1081 CGCGCTATGA CGCATAAAGA CGATAAACGC CACGGTGTTG GGTCGGTTTT TGTTTCACAT
1141 TAAAAC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl