Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/His6-EGFP-Nakanori

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)).

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14301_A8Gop1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Region: 6XHis,thrombin site,EGFP,insert5' Sequence file: RDB14301_A8Goa.seq
>14301_14301_A8Go_T7_20bp_A02_02_ABI24.ab1     1 TTCCAAGATC AATTTCGCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACG    61 CATGGGCAGC AGCCATCATC ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCCA   121 TATGGCTAGA GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA   181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC   241 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG   301 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA   361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC   421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA   481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT   541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA   601 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA   661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA   721 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA   781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA   841 CAAGAAGCTT GGAGGAGGAG GAGGAGAATT CATGCTTTAC GGCGTCGAGA TAGACGAGCA   901 GTACCTGCGC GTGATGGAGG AGTACAAGGA CAAGGAAAGT AATCACGCAG GCCGACATGG   961 CCAAGGTCGC GCTACAACGC AAGAACGTGT ACCAGGACCA GGCCGAGGAA GCGCCAGGCT  1021 GAGCTTAAAG GCCCGAAATA TGGGAGTCGG TGTCTTGTGT CCTCGTGCCG TGTTGTTACA 1081 ATTGCTACTG GGTCGGATCC CCCGAATC //
Primer: T7 terminator Region: insert3' Sequence file: RDB14301_A8Gob.seq
>14301_14301_A8Go_T7_terminator_B02_05_ABI24.ab1     1 GAATGCTTCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGGGAT    61 CCTCAACGGG AGTACACATA CTCCACGTAA GCGGTAGTCT CATTTCCAGT CGACCCGTGA   121 GCCTTGACAT CGCCGTAGCT CGAGTCGCTA TTCTGGCCGT TGTGCGTGTC GAGTAGGTGG   181 AGCATATAGT CCTTGCTACC GTTCTTCCAC CAATGACCGC TCTGGCGCAC CTCAACGAGC   241 ACCTGAGAAT CCCACGAGCG GTTCCAGGGG TTCTGCCAGC CTGAGAAGAT GTCGAGGTTG   301 TCCGTGGTGC CATACACGAT GCACCCCGCG GACCCGACTG CGGCGCCCGC CGACTTGGTG   361 TGGATGAACA CCGTCCATTG CCCATTCCCG ATGCGCTTTT CTCGAGTGTG CGCGCCAAAG   421 TGGCCTCGGA AGGACTCCTC GATCTTCGCT GTGATCGGGC CGCCAGTAGC ATTGTACACA   481 CGCACGAGGA CACAGACACC GACTCCATAT TCGGCCTTAA GCTCAGCCTG GCGCTTCTCG   541 GCCTGGTCCT GGTACACGTT CTTGCGTTGT AGCGCGACCT TGGCCATGTC GGCCTGCGTG   601 ATTACTTCCT TGTCCTTGTA CTCCTCCATC ACGCGCAGGT ACTGCTCGTC TATCTCGACG   661 CCGTAAAGCA TGAATTCTCC TCCTCCTCCT CCAAGCTTCT TGTACAGCTC GTCCATGCCG   721 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG   781 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG   841 CCGTCGCCGA TGGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC   901 GATGTTGTGG GCGGGATCTT GAAGGTTCAC CTTTGATGCC GTTCTTTCTG CTTTGTCGGG   961 CCATGATATA AGACGTTGTG GCTGTTTGTA GGTGTACTCC AAGCTTGTGC CCCCAGGATG  1021 GTTGCCGGTC CTCCATGGAA GTTCGAATGG CCCTTCCAGC TCGAATGCGG TCACAAGGTG 1081 TTCGACCTCG CACCTCACCT CCGGCGCGCG GGGACTA //
Primer: lacIpro_F3 Region: lacI pro,lacI 5' Sequence file: RDB14301_A8Goc.seq
>14301_14301_B0CcS_lacIpro_F3_H04_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCTGGGC TGCTTCCTAA TGCAGGANTC GCATAAGGGA    61 GAGCGTCGAG ATCCCGGNCA CCATCGAATG GCGCAAAACC TTTCGCGGTA TGGCATGATA   121 GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT   181 CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA   241 CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC   301 CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC   361 CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA   421 ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC   481 GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA   541 TGANCAGNAT GCCATTGCTG TGNAAGCTGC CTGCACTAAT GTTCCGGCGT TANTTCNTGA   601 TGTCTCTGAN NNGACACCCN TCNACAGTAN NANTTTCNCC CATGANNANN GNACNCGANN   661 NNNNTNGNNC ATCTGGNCNN ATTNNNNCNN NNNCAAATCG CGCTNNNNNC GGNNNNNTTN 721 NGNNCNGNNT CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl