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pET28/His6-EGFP-Nakanori

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.

Catalog number RDB14301
Resource name pET28/His6-EGFP-Nakanori
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. Nakanori is non-toxic mushroom protein that specifically binds a complex of sphingomyelin.
Comment Expression was confirmed by the depositor with SDS-PAGE and confocal micriscopy. AB700589.1 for Grifola frondosa Nakanori mRNA for hypothetical protein, complete cds.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Grifola frondosa Nakanori cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
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External Database
Grifola frondosa Nakanori

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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            RDB14301 pET28/His6-EGFP-Nakanori DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0062e (English text)
            Featured content Dnaconda's recommendation EXR0062j (Japanese text)
            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14301_A8Gop1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Region: 6XHis,thrombin site,EGFP,insert5'
            Sequence file: RDB14301_A8Goa.seq check
            >14301_14301_A8Go_T7_20bp_A02_02_ABI24.ab1
                1 TTCCAAGATC AATTTCGCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACG
               61 CATGGGCAGC AGCCATCATC ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCCA
              121 TATGGCTAGA GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
              181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
              241 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
              301 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
              361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
              421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
              481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
              541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
              601 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
              661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
              721 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
              781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
              841 CAAGAAGCTT GGAGGAGGAG GAGGAGAATT CATGCTTTAC GGCGTCGAGA TAGACGAGCA
              901 GTACCTGCGC GTGATGGAGG AGTACAAGGA CAAGGAAAGT AATCACGCAG GCCGACATGG
              961 CCAAGGTCGC GCTACAACGC AAGAACGTGT ACCAGGACCA GGCCGAGGAA GCGCCAGGCT
             1021 GAGCTTAAAG GCCCGAAATA TGGGAGTCGG TGTCTTGTGT CCTCGTGCCG TGTTGTTACA
            1081 ATTGCTACTG GGTCGGATCC CCCGAATC
            //
            Primer: T7 terminator
            Region: insert3'
            Sequence file: RDB14301_A8Gob.seq check
            >14301_14301_A8Go_T7_terminator_B02_05_ABI24.ab1
                1 GAATGCTTCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGGGAT
               61 CCTCAACGGG AGTACACATA CTCCACGTAA GCGGTAGTCT CATTTCCAGT CGACCCGTGA
              121 GCCTTGACAT CGCCGTAGCT CGAGTCGCTA TTCTGGCCGT TGTGCGTGTC GAGTAGGTGG
              181 AGCATATAGT CCTTGCTACC GTTCTTCCAC CAATGACCGC TCTGGCGCAC CTCAACGAGC
              241 ACCTGAGAAT CCCACGAGCG GTTCCAGGGG TTCTGCCAGC CTGAGAAGAT GTCGAGGTTG
              301 TCCGTGGTGC CATACACGAT GCACCCCGCG GACCCGACTG CGGCGCCCGC CGACTTGGTG
              361 TGGATGAACA CCGTCCATTG CCCATTCCCG ATGCGCTTTT CTCGAGTGTG CGCGCCAAAG
              421 TGGCCTCGGA AGGACTCCTC GATCTTCGCT GTGATCGGGC CGCCAGTAGC ATTGTACACA
              481 CGCACGAGGA CACAGACACC GACTCCATAT TCGGCCTTAA GCTCAGCCTG GCGCTTCTCG
              541 GCCTGGTCCT GGTACACGTT CTTGCGTTGT AGCGCGACCT TGGCCATGTC GGCCTGCGTG
              601 ATTACTTCCT TGTCCTTGTA CTCCTCCATC ACGCGCAGGT ACTGCTCGTC TATCTCGACG
              661 CCGTAAAGCA TGAATTCTCC TCCTCCTCCT CCAAGCTTCT TGTACAGCTC GTCCATGCCG
              721 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
              781 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
              841 CCGTCGCCGA TGGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC
              901 GATGTTGTGG GCGGGATCTT GAAGGTTCAC CTTTGATGCC GTTCTTTCTG CTTTGTCGGG
              961 CCATGATATA AGACGTTGTG GCTGTTTGTA GGTGTACTCC AAGCTTGTGC CCCCAGGATG
             1021 GTTGCCGGTC CTCCATGGAA GTTCGAATGG CCCTTCCAGC TCGAATGCGG TCACAAGGTG
            1081 TTCGACCTCG CACCTCACCT CCGGCGCGCG GGGACTA
            //
            Primer: lacIpro_F3
            Region: lacI pro,lacI 5'
            Sequence file: RDB14301_A8Goc.seq check
            >14301_14301_B0CcS_lacIpro_F3_H04_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNCTGGGC TGCTTCCTAA TGCAGGANTC GCATAAGGGA
               61 GAGCGTCGAG ATCCCGGNCA CCATCGAATG GCGCAAAACC TTTCGCGGTA TGGCATGATA
              121 GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT
              181 CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA
              241 CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC
              301 CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC
              361 CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA
              421 ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC
              481 GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA
              541 TGANCAGNAT GCCATTGCTG TGNAAGCTGC CTGCACTAAT GTTCCGGCGT TANTTCNTGA
              601 TGTCTCTGAN NNGACACCCN TCNACAGTAN NANTTTCNCC CATGANNANN GNACNCGANN
              661 NNNNTNGNNC ATCTGGNCNN ATTNNNNCNN NNNCAAATCG CGCTNNNNNC GGNNNNNTTN
            721 NGNNCNGNNT CNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl