Sequencing and PCR primers for primer information.
Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.
| Catalog number | RDB14301 |
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| Resource name | pET28/His6-EGFP-Nakanori |
| Clone info. | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. Nakanori is non-toxic mushroom protein that specifically binds a complex of sphingomyelin. Expression was confirmed by the depositor with SDS-PAGE and confocal micriscopy. AB700589.1 for Grifola frondosa Nakanori mRNA for hypothetical protein, complete cds. |
| Vector backbone | pET-28b (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Kan^r |
| Gene/insert name | Grifola frondosa Nakanori cDNA |
| Depositor | Sako, Yasushi | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A. et al., FASEB J. 2016 Aug 4. pii: fj.201500075R.) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB14301 | pET28/His6-EGFP-Nakanori | DNA solution |
Electronic file
Featured content
| Featured content | Sphingolipid signaling pathway (English text) |
|---|---|
| Featured content | Dnaconda's recommendation EXR0062e (English text) |
| Featured content | Dnaconda's recommendation EXR0062j (Japanese text) |
| Featured content | Organelle marker (English text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14301_A6Hup1_2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB14301_A6Hua.seq |
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>D03762A2_A6Hu_2_T7_20bp_E11_14_ABI24.ab1 1 TCGGGTCATT CCCATCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG 61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC 121 TAGAGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA 181 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA 241 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC 301 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA 361 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT 421 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT 481 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA 541 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA 601 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC 661 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA 721 CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT 781 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGAA 841 GCTTGGAGGA GGAGGAGGAG AATTCATGCT TTACGGCGTC GAGATAGACG AGCAGTACCT 901 GCGCGTGATG GAGGAGTACA AAGGACAAGG AAGTAATCAC GCAGGCCGAC ATGGCCCAAG 961 GTCGCGCTAC AACGCAAGAA CGTGTACCAG GGACCAGGCC GAGAAGCGCC AGGCTGAGCT 1021 TTAAAGCCCG AATATGGAGT CGTGTCTGTG TCCTCGTGCG TGTGTACATG CTACTGGTGG 1081 GCCCGATCAC AAGCCGAGAT CGAAGAAGTT CTTTCGAGGC CAACTTGGCG GCGCACAACC 1141 T // |
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| Primer: T7_terminator | RDB14301_A6Hub.seq |
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>D03762A2_A6Hu_2_T7 Terminator_F11_17_ABI24.ab1 1 GGCTCGCCTT TCTTTTCGGG CTTTGTTAGC AAGCCGGATC TCAGTGGTGG TGGTGGTGGT 61 GGGATCCTCA ACGGGAGTAC ACATACTCCA CGTAAGCGGT AGTCTCATTT CCAGTCGACC 121 CGTGAGCCTT GACATCGCCG TAGCTCGAGT CGCTATTCTG GCCGTTGTGC GTGTCGAGTA 181 GGTGGAGCAT ATAGTCCTTG CTACCGTTCT TCCACCAATG ACCGCTCTGG CGCACCTCAA 241 CGAGCACCTG AGAATCCCAC GAGCGGTTCC AGGGGTTCTG CCAGCCTGAG AAGATGTCGA 301 GGTTGTCCGT GGTGCCATAC ACGATGCACC CCGCGGACCC GACTGCGGCG CCCGCCGACT 361 TGGTGTGGAT GAACACCGTC CATTGCCCAT TCCCGATGCG CTTTTCTCGA GTGTGCGCGC 421 CAAAGTGGCC TCGGAAGGAC TCCTCGATCT TCGCTGTGAT CGGGCCGCCA GTAGCATTGT 481 ACACACGCAC GAGGACACAG ACACCGACTC CATATTCGGC CTTAAGCTCA GCCTGGCGCT 541 TCTCGGCCTG GTCCTGGTAC ACGTTCTTGC GTTGTAGCGC GACCTTGGCC ATGTCGGCCT 601 GCGTGATTAC TTCCTTGTCC TTGTACTCCT CCATCACGCG CAGGTACTGC TCGTCTATCT 661 CGACGCCGTA AAGCATGAAT TCTCCTCCTC CTCCTCCAAG CTTCTTGTAC AGCTCGTCCA 721 TGCCGAGAGT GATCCCGGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC 781 TCGTTGGGGT CTTTGCTCAG GGCGGGACTG GGGTGCTCAG GTAGTGGTTG TCGGGCAGCA 841 GCACGGGGGC CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG 901 CCGTCCTCGA TGTTTGTGGC GGGATCTTGA AGTTCACCTT GATGCCCGTT TCTTTCTGCT 961 TGTCGGCCAT GATATAGACG TTTGTGGCTG TTTGTAGTTG TACTTCAAGC TTGTGCCCCA 1021 AGGATGTTGG CCGTTCCTCC TTTGAAGTCG AATGCCCTTT CAGCCTCGAT GCGGATTTCA 1081 CCCAGGGTGT TCGCCCTCGG ACTTTCAACC CTTCCGAGCG CGGGTCTTTG TAGTTGACGG 1141 TTCGTTCCTT GAAAAAAGAT TGGGTCCGCC CTTCCCTGTG AG // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.11
GNP_filter3_RDBDEP_html_181012.pl