Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.
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Clone info. | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. Nakanori is non-toxic mushroom protein that specifically binds a complex of sphingomyelin. |
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Comment | Expression was confirmed by the depositor with SDS-PAGE and confocal micriscopy. AB700589.1 for Grifola frondosa Nakanori mRNA for hypothetical protein, complete cds. |
Vector backbone | pET-28b (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Kan^r |
Growth conditions | LB+Kanamycin, 37oC |
Gene/insert name | Grifola frondosa Nakanori cDNA |
Depositor|Developer | Sako, Yasushi | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。 |
Catalog # | Resource name | Shipping form | Fee |
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RDB14301 | pET28/His6-EGFP-Nakanori | DNA solution |
Materials & Methods section:
The pET28/His6-EGFP-Nakanori was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14301). |
Reference section:
Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.G., Kasai, R.S., Kubo, S., Schieber, N.L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton R.G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP] |
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