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pET28/His6-EGFP-Nakanori (#RDB14301)

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Makino, A., FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. Nakanori is non-toxic mushroom protein that specifically binds a complex of sphingomyelin.
Comment Expression was confirmed by the depositor with SDS-PAGE and confocal micriscopy. AB700589.1 for Grifola frondosa Nakanori mRNA for hypothetical protein, complete cds.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Growth conditions LB+Kanamycin, 37oC
Gene/insert name Grifola frondosa Nakanori cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。

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RDB14301 pET28/His6-EGFP-Nakanori DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/His6-EGFP-Nakanori was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14301).

Reference section:

Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.G., Kasai, R.S., Kubo, S., Schieber, N.L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton R.G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [PubMed] [Article] [RRC of NBRP]