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GL207

Expression plasmid of GFP-tagged human cRaf1 CRDCAD (R89A S621A).

Catalog number RDB13256
Resource name GL207
Clone info. Expression plasmid of human cRaf1 CRDCAD (R89A S621A). See Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.
Comment monomeric EGFP.
Vector backbone pmEGFP-C2-NheI (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human RAF1 cDNA
Depositor|Developer Sako, Yasushi |
Other clones in our bank

External Database
Human RAF1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 286 (42): 36460-36468, 2011)。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13256 GL207 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content MAP kinases (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13256_A5D8.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13256_A5D8a.seq check
          >13256_13256_A5D8_2_CMV-Forward_F10_16.ab1
              1 CAACCACCCT CCCGGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GATCTCGATA GCGCTACCGG
            121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
            181 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG
            241 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
            301 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
            361 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA
            421 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
            481 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
            541 TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC
            601 AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC
            661 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG
            721 ACAACCACTA CCTGAGCACC CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC
            781 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT
            841 ACAAGTCCGG CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGGAG CACATACAGG
            901 GAGCTTGGAA GACGATCAGC AATGGTTTTG GATTCAAAGA TGCCGTGTTT GATGGCTCCA
            961 GCTGCATCTC TCCTACAATA GTTCAGCAGT TTGGCTATCA GCGCCGGGCA TCAGATGATG
           1021 GCAACTCACA GATCCCTTCT AAGACAAGCA ACACTATCCG TGTTTTCTTG GCCGACAGCA
           1081 AGACAGTGGA TTCAATGTGC GAAATGGATT GAGCTTGCAA TGAACTGCCT TAATGAAAGG
          1141 CCACCTCCAA
          //
          Primer: EGFP-C
          Sequence file: RDB13256_A5D8b.seq check
          >13256_13256_A5D8_2_EGFP-C_G10_19.ab1
              1 CGGGAATTTC TTCGGGCATG GACGAGCTGT ACAAGTCCGG CCGGACTCAG ATCTCGAGCT
             61 CAAGCTTCGA ATTCATGGAG CACATACAGG GAGCTTGGAA GACGATCAGC AATGGTTTTG
            121 GATTCAAAGA TGCCGTGTTT GATGGCTCCA GCTGCATCTC TCCTACAATA GTTCAGCAGT
            181 TTGGCTATCA GCGCCGGGCA TCAGATGATG GCAAACTCAC AGATCCTTCT AAGACAAGCA
            241 ACACTATCCG TGTTTTCTTG CCGAACAAGC AAAGAACAGT GGTCAATGTG CGAAATGGAA
            301 TGAGCTTGCA TGACTGCCTT ATGAAAGCAC TCAAGGTGGC GGGCCTGCAA CCAGAGTGCT
            361 GTGCAGTGTT CAGACTTCTC CACGAACACA AAGGTAAAAA AGCACGCTTA GATTGGAATA
            421 CTGATGCTGC GTCTTTGATT GGAGAAGAAC TTCAAGTAGA TTTCCTGGAT CATGTTCCCC
            481 TCACAACACA CAACTTTGCT CGGAAGACGT TCCTGAAGCT TGCCTTCTGT GACATCTGTC
            541 AGAAATTCCT GCTCAATGGA TTTCGATGTC AGACTTGTGG CTACAAATTT CATGAGCACT
            601 GTAGCACCAA AGTACCTACT ATGTGTGTGG ACTGGAGTAA CATCAGACAA CTCTTATTGT
            661 TTCCAAATTC CACTATTGGT GATAGTGGAG TCCCAGCACT ACCTTCTTTG ACTATGCGTC
            721 GTATGCGAGA GTCTGTTTCC AGGATGCCTG TTAGTTCTCA GCACAGATAT TCTACACCTC
            781 ACGCCTTCAC CTTTAACACC TCCAGTCCCT CATCTGAAGG TTCCCTCTCC CAGAGGCAGA
            841 GGTCGACATC CACACCTAAT GTCCACATGG TCAGCACCAC CCTGCCTGTG GACAGCAGGA
            901 TGATTGAGGA TGCAATTCGA AGTCACAGCG AATCGGCCTC ACCTTCAGCC CTGTCCAGTA
            961 GCCCCAACAA TCTGAGCCCA ACAGGCTGGT CACAGCCGAA AACCCCCGTG CCAGCACAAG
           1021 AGAGCGGGCA CCAGTATCTG GGACCCAGAG AAAACAATTA GGCCTCGTGG ACGAGAGATC
           1081 CAGCCTATAT GGGAAATAGA GCCAGTGAAG TGATGCTGTC CACCTCGGAT GGGGTCAGGC
          1141 CTTCTTTTTG GCAACCTGG
          //
          Primer: pAxEF_R2
          Sequence file: RDB13256_A5D8c.seq check
          >13256_13256_A5D8_2_pAxEF_R2_C05_08.ab1
              1 CGGGTAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
             61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
            121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
            181 ATCAGTTATC TAGATCCGGT GGATCCCTAG AAGACAGGCA GCCTCGGGGA CGTGGTCAGC
            241 GTGCAAGCAT TGATATCCTC AGTGTGGGCT GCCCGATGCA AGGATGGCTC GGCAGCGCTC
            301 CGGTTGATCT TCGGTAGAGA GTGTTGGAGC AGCTCAATGG AAGACAGGAT CTGGGGAAAA
            361 AGAGGCCTCT CTTCCTTTAC TTTCTTCACA CAGTCAGCTA CCAGCCTCTT CATTGCTTTG
            421 GGGCAGTTCT TATATAGCTT ACTAAGATCT GGGGAGGCAT ATCCTCGGCC CACCATGAAG
            481 ATGATCTGAT CTCGGTTGTT GATGTGAGAA TAAGGAAGCT CCCCCGTCAT CAGTTCATAC
            541 AATACGATGC CATAGGAGTA GACATCCGAC TGGAAACTGA ATGGGTTGTT ATCCTGCATT
            601 CGGATCACCT CTGGGGCCAT CCAGAGGACA GAGCCAGTAG GTTGTTCAAC CTGCTGAGAA
            661 CCACTCCAGC GTGACTTTAC TGTTGCCAAA CCAAAATCTC CAATTTTCAC TGTTAAGCCT
            721 TCATGGAGAA ATATATTGTT GGATTTCATG TCTCTATGGA TGATGTTCTT TGCATGCAAA
            781 TAGTCCATTC CCTGAGCCGT CTGCCGGGCA ATGTCAATTA GCTGGAACAT CTGAAACTTG
            841 GTCTCCTGGA CATGCAGGTG TTTGTAGAGG CTGCTGCCCT CGCACCACTG GGTCACAATT
            901 GCCAGGTTGT CCTTTGTCAT GTACCCCATG AAAAGCAGAA TGTTCACATG CCGTGTTTTG
            961 CGCAGAACAG CCACCTCATT CCTGAAGGCC TGGAATTTGC TCTGGGGGTT GGGTCGACAA
           1021 CCCTTTAGGA TCTTTACTGC AACATCTCCG TGCCCATTTA CCCTTTATAA ACAGACCAAA
           1081 GAGCCTGACC CACTCGAATG ACAGCCATCA CCTTCACTGC CTCCTATTTC CGAATATAGC
          1141 TGCAATCTCT CTGCATCCGC AG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13256_A5D8d.seq check
          >13256_13256_A5D8_2_SV40proF_V2_A11_02.ab1
              1 TAAGAGCTTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
             61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
            601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
            901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
            961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGGA
           1021 GTTCTTCGCC ACCCTAGGGG AGCTAACTGA AACACGGAAG GAGACATACC GGGAGACCCG
           1081 CGCTATGACG GCAATAAAGA CCGATTAAAC GCACGTGTGG GTCGGTTTGT TTCATAAACG
          1141 GCCGGGGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl