Please review the QC test results indicated by icon below as well as clone information before placing your order.

GL207

Expression plasmid of GFP-tagged human cRaf1 CRDCAD (R89A S621A).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13256_A5D8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13256_A5D8a.seq
>13256_13256_A5D8_2_CMV-Forward_F10_16.ab1     1 CAACCACCCT CCCGGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GATCTCGATA GCGCTACCGG   121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG   181 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG   241 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT   301 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC   361 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA   421 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG   481 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC   541 TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC   601 AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC   661 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG   721 ACAACCACTA CCTGAGCACC CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC   781 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT   841 ACAAGTCCGG CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGGAG CACATACAGG   901 GAGCTTGGAA GACGATCAGC AATGGTTTTG GATTCAAAGA TGCCGTGTTT GATGGCTCCA   961 GCTGCATCTC TCCTACAATA GTTCAGCAGT TTGGCTATCA GCGCCGGGCA TCAGATGATG  1021 GCAACTCACA GATCCCTTCT AAGACAAGCA ACACTATCCG TGTTTTCTTG GCCGACAGCA  1081 AGACAGTGGA TTCAATGTGC GAAATGGATT GAGCTTGCAA TGAACTGCCT TAATGAAAGG 1141 CCACCTCCAA //
Primer: EGFP-C Sequence file: RDB13256_A5D8b.seq
>13256_13256_A5D8_2_EGFP-C_G10_19.ab1     1 CGGGAATTTC TTCGGGCATG GACGAGCTGT ACAAGTCCGG CCGGACTCAG ATCTCGAGCT    61 CAAGCTTCGA ATTCATGGAG CACATACAGG GAGCTTGGAA GACGATCAGC AATGGTTTTG   121 GATTCAAAGA TGCCGTGTTT GATGGCTCCA GCTGCATCTC TCCTACAATA GTTCAGCAGT   181 TTGGCTATCA GCGCCGGGCA TCAGATGATG GCAAACTCAC AGATCCTTCT AAGACAAGCA   241 ACACTATCCG TGTTTTCTTG CCGAACAAGC AAAGAACAGT GGTCAATGTG CGAAATGGAA   301 TGAGCTTGCA TGACTGCCTT ATGAAAGCAC TCAAGGTGGC GGGCCTGCAA CCAGAGTGCT   361 GTGCAGTGTT CAGACTTCTC CACGAACACA AAGGTAAAAA AGCACGCTTA GATTGGAATA   421 CTGATGCTGC GTCTTTGATT GGAGAAGAAC TTCAAGTAGA TTTCCTGGAT CATGTTCCCC   481 TCACAACACA CAACTTTGCT CGGAAGACGT TCCTGAAGCT TGCCTTCTGT GACATCTGTC   541 AGAAATTCCT GCTCAATGGA TTTCGATGTC AGACTTGTGG CTACAAATTT CATGAGCACT   601 GTAGCACCAA AGTACCTACT ATGTGTGTGG ACTGGAGTAA CATCAGACAA CTCTTATTGT   661 TTCCAAATTC CACTATTGGT GATAGTGGAG TCCCAGCACT ACCTTCTTTG ACTATGCGTC   721 GTATGCGAGA GTCTGTTTCC AGGATGCCTG TTAGTTCTCA GCACAGATAT TCTACACCTC   781 ACGCCTTCAC CTTTAACACC TCCAGTCCCT CATCTGAAGG TTCCCTCTCC CAGAGGCAGA   841 GGTCGACATC CACACCTAAT GTCCACATGG TCAGCACCAC CCTGCCTGTG GACAGCAGGA   901 TGATTGAGGA TGCAATTCGA AGTCACAGCG AATCGGCCTC ACCTTCAGCC CTGTCCAGTA   961 GCCCCAACAA TCTGAGCCCA ACAGGCTGGT CACAGCCGAA AACCCCCGTG CCAGCACAAG  1021 AGAGCGGGCA CCAGTATCTG GGACCCAGAG AAAACAATTA GGCCTCGTGG ACGAGAGATC  1081 CAGCCTATAT GGGAAATAGA GCCAGTGAAG TGATGCTGTC CACCTCGGAT GGGGTCAGGC 1141 CTTCTTTTTG GCAACCTGG //
Primer: pAxEF_R2 Sequence file: RDB13256_A5D8c.seq
>13256_13256_A5D8_2_pAxEF_R2_C05_08.ab1     1 CGGGTAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA    61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG   121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG   181 ATCAGTTATC TAGATCCGGT GGATCCCTAG AAGACAGGCA GCCTCGGGGA CGTGGTCAGC   241 GTGCAAGCAT TGATATCCTC AGTGTGGGCT GCCCGATGCA AGGATGGCTC GGCAGCGCTC   301 CGGTTGATCT TCGGTAGAGA GTGTTGGAGC AGCTCAATGG AAGACAGGAT CTGGGGAAAA   361 AGAGGCCTCT CTTCCTTTAC TTTCTTCACA CAGTCAGCTA CCAGCCTCTT CATTGCTTTG   421 GGGCAGTTCT TATATAGCTT ACTAAGATCT GGGGAGGCAT ATCCTCGGCC CACCATGAAG   481 ATGATCTGAT CTCGGTTGTT GATGTGAGAA TAAGGAAGCT CCCCCGTCAT CAGTTCATAC   541 AATACGATGC CATAGGAGTA GACATCCGAC TGGAAACTGA ATGGGTTGTT ATCCTGCATT   601 CGGATCACCT CTGGGGCCAT CCAGAGGACA GAGCCAGTAG GTTGTTCAAC CTGCTGAGAA   661 CCACTCCAGC GTGACTTTAC TGTTGCCAAA CCAAAATCTC CAATTTTCAC TGTTAAGCCT   721 TCATGGAGAA ATATATTGTT GGATTTCATG TCTCTATGGA TGATGTTCTT TGCATGCAAA   781 TAGTCCATTC CCTGAGCCGT CTGCCGGGCA ATGTCAATTA GCTGGAACAT CTGAAACTTG   841 GTCTCCTGGA CATGCAGGTG TTTGTAGAGG CTGCTGCCCT CGCACCACTG GGTCACAATT   901 GCCAGGTTGT CCTTTGTCAT GTACCCCATG AAAAGCAGAA TGTTCACATG CCGTGTTTTG   961 CGCAGAACAG CCACCTCATT CCTGAAGGCC TGGAATTTGC TCTGGGGGTT GGGTCGACAA  1021 CCCTTTAGGA TCTTTACTGC AACATCTCCG TGCCCATTTA CCCTTTATAA ACAGACCAAA  1081 GAGCCTGACC CACTCGAATG ACAGCCATCA CCTTCACTGC CTCCTATTTC CGAATATAGC 1141 TGCAATCTCT CTGCATCCGC AG //
Primer: SV40pro_F_V2 Sequence file: RDB13256_A5D8d.seq
>13256_13256_A5D8_2_SV40proF_V2_A11_02.ab1     1 TAAGAGCTTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC    61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG   901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC   961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGGA  1021 GTTCTTCGCC ACCCTAGGGG AGCTAACTGA AACACGGAAG GAGACATACC GGGAGACCCG  1081 CGCTATGACG GCAATAAAGA CCGATTAAAC GCACGTGTGG GTCGGTTTGT TTCATAAACG 1141 GCCGGGGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl