Resource data sheet
Prev.

GL207

Expression plasmid of GFP-tagged human cRaf1 CRDCAD (R89A S621A).

Catalog number RDB13256
Resource name GL207
Clone info. Expression plasmid of human cRaf1 CRDCAD (R89A S621A). See Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.
monomeric EGFP.
Vector backbone pmEGFP-C2-NheI (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human RAF1 cDNA
Depositor Sako, Yasushi |
 
Other clones in our bank human RAF1 (NCBI Gene 5894) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011)
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13256 GL207 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content MAP kinases (English text)

References

original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13256_A5D8.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13256_A5D8a.seq
>13256_13256_A5D8_2_CMV-Forward_F10_16.ab1
    1 CAACCACCCT CCCGGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GATCTCGATA GCGCTACCGG
  121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
  181 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG
  241 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
  301 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
  361 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA
  421 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
  481 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
  541 TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC
  601 AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC
  661 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG
  721 ACAACCACTA CCTGAGCACC CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC
  781 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT
  841 ACAAGTCCGG CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGGAG CACATACAGG
  901 GAGCTTGGAA GACGATCAGC AATGGTTTTG GATTCAAAGA TGCCGTGTTT GATGGCTCCA
  961 GCTGCATCTC TCCTACAATA GTTCAGCAGT TTGGCTATCA GCGCCGGGCA TCAGATGATG
 1021 GCAACTCACA GATCCCTTCT AAGACAAGCA ACACTATCCG TGTTTTCTTG GCCGACAGCA
 1081 AGACAGTGGA TTCAATGTGC GAAATGGATT GAGCTTGCAA TGAACTGCCT TAATGAAAGG
1141 CCACCTCCAA
//
Primer: EGFP-C RDB13256_A5D8b.seq
>13256_13256_A5D8_2_EGFP-C_G10_19.ab1
    1 CGGGAATTTC TTCGGGCATG GACGAGCTGT ACAAGTCCGG CCGGACTCAG ATCTCGAGCT
   61 CAAGCTTCGA ATTCATGGAG CACATACAGG GAGCTTGGAA GACGATCAGC AATGGTTTTG
  121 GATTCAAAGA TGCCGTGTTT GATGGCTCCA GCTGCATCTC TCCTACAATA GTTCAGCAGT
  181 TTGGCTATCA GCGCCGGGCA TCAGATGATG GCAAACTCAC AGATCCTTCT AAGACAAGCA
  241 ACACTATCCG TGTTTTCTTG CCGAACAAGC AAAGAACAGT GGTCAATGTG CGAAATGGAA
  301 TGAGCTTGCA TGACTGCCTT ATGAAAGCAC TCAAGGTGGC GGGCCTGCAA CCAGAGTGCT
  361 GTGCAGTGTT CAGACTTCTC CACGAACACA AAGGTAAAAA AGCACGCTTA GATTGGAATA
  421 CTGATGCTGC GTCTTTGATT GGAGAAGAAC TTCAAGTAGA TTTCCTGGAT CATGTTCCCC
  481 TCACAACACA CAACTTTGCT CGGAAGACGT TCCTGAAGCT TGCCTTCTGT GACATCTGTC
  541 AGAAATTCCT GCTCAATGGA TTTCGATGTC AGACTTGTGG CTACAAATTT CATGAGCACT
  601 GTAGCACCAA AGTACCTACT ATGTGTGTGG ACTGGAGTAA CATCAGACAA CTCTTATTGT
  661 TTCCAAATTC CACTATTGGT GATAGTGGAG TCCCAGCACT ACCTTCTTTG ACTATGCGTC
  721 GTATGCGAGA GTCTGTTTCC AGGATGCCTG TTAGTTCTCA GCACAGATAT TCTACACCTC
  781 ACGCCTTCAC CTTTAACACC TCCAGTCCCT CATCTGAAGG TTCCCTCTCC CAGAGGCAGA
  841 GGTCGACATC CACACCTAAT GTCCACATGG TCAGCACCAC CCTGCCTGTG GACAGCAGGA
  901 TGATTGAGGA TGCAATTCGA AGTCACAGCG AATCGGCCTC ACCTTCAGCC CTGTCCAGTA
  961 GCCCCAACAA TCTGAGCCCA ACAGGCTGGT CACAGCCGAA AACCCCCGTG CCAGCACAAG
 1021 AGAGCGGGCA CCAGTATCTG GGACCCAGAG AAAACAATTA GGCCTCGTGG ACGAGAGATC
 1081 CAGCCTATAT GGGAAATAGA GCCAGTGAAG TGATGCTGTC CACCTCGGAT GGGGTCAGGC
1141 CTTCTTTTTG GCAACCTGG
//
Primer: pAxEF_R2 RDB13256_A5D8c.seq
>13256_13256_A5D8_2_pAxEF_R2_C05_08.ab1
    1 CGGGTAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCAGTTATC TAGATCCGGT GGATCCCTAG AAGACAGGCA GCCTCGGGGA CGTGGTCAGC
  241 GTGCAAGCAT TGATATCCTC AGTGTGGGCT GCCCGATGCA AGGATGGCTC GGCAGCGCTC
  301 CGGTTGATCT TCGGTAGAGA GTGTTGGAGC AGCTCAATGG AAGACAGGAT CTGGGGAAAA
  361 AGAGGCCTCT CTTCCTTTAC TTTCTTCACA CAGTCAGCTA CCAGCCTCTT CATTGCTTTG
  421 GGGCAGTTCT TATATAGCTT ACTAAGATCT GGGGAGGCAT ATCCTCGGCC CACCATGAAG
  481 ATGATCTGAT CTCGGTTGTT GATGTGAGAA TAAGGAAGCT CCCCCGTCAT CAGTTCATAC
  541 AATACGATGC CATAGGAGTA GACATCCGAC TGGAAACTGA ATGGGTTGTT ATCCTGCATT
  601 CGGATCACCT CTGGGGCCAT CCAGAGGACA GAGCCAGTAG GTTGTTCAAC CTGCTGAGAA
  661 CCACTCCAGC GTGACTTTAC TGTTGCCAAA CCAAAATCTC CAATTTTCAC TGTTAAGCCT
  721 TCATGGAGAA ATATATTGTT GGATTTCATG TCTCTATGGA TGATGTTCTT TGCATGCAAA
  781 TAGTCCATTC CCTGAGCCGT CTGCCGGGCA ATGTCAATTA GCTGGAACAT CTGAAACTTG
  841 GTCTCCTGGA CATGCAGGTG TTTGTAGAGG CTGCTGCCCT CGCACCACTG GGTCACAATT
  901 GCCAGGTTGT CCTTTGTCAT GTACCCCATG AAAAGCAGAA TGTTCACATG CCGTGTTTTG
  961 CGCAGAACAG CCACCTCATT CCTGAAGGCC TGGAATTTGC TCTGGGGGTT GGGTCGACAA
 1021 CCCTTTAGGA TCTTTACTGC AACATCTCCG TGCCCATTTA CCCTTTATAA ACAGACCAAA
 1081 GAGCCTGACC CACTCGAATG ACAGCCATCA CCTTCACTGC CTCCTATTTC CGAATATAGC
1141 TGCAATCTCT CTGCATCCGC AG
//
Primer: SV40pro_F_V2 RDB13256_A5D8d.seq
>13256_13256_A5D8_2_SV40proF_V2_A11_02.ab1
    1 TAAGAGCTTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
  961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGGA
 1021 GTTCTTCGCC ACCCTAGGGG AGCTAACTGA AACACGGAAG GAGACATACC GGGAGACCCG
 1081 CGCTATGACG GCAATAAAGA CCGATTAAAC GCACGTGTGG GTCGGTTTGT TTCATAAACG
1141 GCCGGGGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl