Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
GL207 (#RDB13256)
Expression plasmid of GFP-tagged human cRaf1 CRDCAD (R89A S621A).
| Clone info. | Expression plasmid of human cRaf1 CRDCAD (R89A S621A). See Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. |
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| Comment | monomeric EGFP. |
| Vector backbone | pmEGFP-C2-NheI (Plasmid) |
| Size of vector backbone | 4.7 kb |
| Selectable markers | Kan^r |
| Gene/insert name | Human RAF1 cDNA |
| Depositor|Developer | Sako, Yasushi | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (J. Biol. Chem. 286 (42): 36460-36468, 2011) |
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[open/close]| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 286 (42): 36460-36468, 2011)。 |
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| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13256 | GL207 | DNA solution |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The GL207 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13256). |
Reference section:
| Hibino, K., Shibata, T., Yanagida, T., Sako, Y., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13256_A5D8.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB13256_A5D8a.seq
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>13256_13256_A5D8_2_CMV-Forward_F10_16.ab1 1 CAACCACCCT CCCGGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GATCTCGATA GCGCTACCGG 121 TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG 181 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG 241 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT 301 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC 361 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA 421 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG 481 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC 541 TGGGGCACAA GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC 601 AGAAGAACGG CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC 661 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG 721 ACAACCACTA CCTGAGCACC CAGTCCAAGC TGAGCAAAGA CCCCAACGAG AAGCGCGATC 781 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT 841 ACAAGTCCGG CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCATGGAG CACATACAGG 901 GAGCTTGGAA GACGATCAGC AATGGTTTTG GATTCAAAGA TGCCGTGTTT GATGGCTCCA 961 GCTGCATCTC TCCTACAATA GTTCAGCAGT TTGGCTATCA GCGCCGGGCA TCAGATGATG 1021 GCAACTCACA GATCCCTTCT AAGACAAGCA ACACTATCCG TGTTTTCTTG GCCGACAGCA 1081 AGACAGTGGA TTCAATGTGC GAAATGGATT GAGCTTGCAA TGAACTGCCT TAATGAAAGG 1141 CCACCTCCAA // |
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Primer: EGFP-C Sequence file: RDB13256_A5D8b.seq
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>13256_13256_A5D8_2_EGFP-C_G10_19.ab1 1 CGGGAATTTC TTCGGGCATG GACGAGCTGT ACAAGTCCGG CCGGACTCAG ATCTCGAGCT 61 CAAGCTTCGA ATTCATGGAG CACATACAGG GAGCTTGGAA GACGATCAGC AATGGTTTTG 121 GATTCAAAGA TGCCGTGTTT GATGGCTCCA GCTGCATCTC TCCTACAATA GTTCAGCAGT 181 TTGGCTATCA GCGCCGGGCA TCAGATGATG GCAAACTCAC AGATCCTTCT AAGACAAGCA 241 ACACTATCCG TGTTTTCTTG CCGAACAAGC AAAGAACAGT GGTCAATGTG CGAAATGGAA 301 TGAGCTTGCA TGACTGCCTT ATGAAAGCAC TCAAGGTGGC GGGCCTGCAA CCAGAGTGCT 361 GTGCAGTGTT CAGACTTCTC CACGAACACA AAGGTAAAAA AGCACGCTTA GATTGGAATA 421 CTGATGCTGC GTCTTTGATT GGAGAAGAAC TTCAAGTAGA TTTCCTGGAT CATGTTCCCC 481 TCACAACACA CAACTTTGCT CGGAAGACGT TCCTGAAGCT TGCCTTCTGT GACATCTGTC 541 AGAAATTCCT GCTCAATGGA TTTCGATGTC AGACTTGTGG CTACAAATTT CATGAGCACT 601 GTAGCACCAA AGTACCTACT ATGTGTGTGG ACTGGAGTAA CATCAGACAA CTCTTATTGT 661 TTCCAAATTC CACTATTGGT GATAGTGGAG TCCCAGCACT ACCTTCTTTG ACTATGCGTC 721 GTATGCGAGA GTCTGTTTCC AGGATGCCTG TTAGTTCTCA GCACAGATAT TCTACACCTC 781 ACGCCTTCAC CTTTAACACC TCCAGTCCCT CATCTGAAGG TTCCCTCTCC CAGAGGCAGA 841 GGTCGACATC CACACCTAAT GTCCACATGG TCAGCACCAC CCTGCCTGTG GACAGCAGGA 901 TGATTGAGGA TGCAATTCGA AGTCACAGCG AATCGGCCTC ACCTTCAGCC CTGTCCAGTA 961 GCCCCAACAA TCTGAGCCCA ACAGGCTGGT CACAGCCGAA AACCCCCGTG CCAGCACAAG 1021 AGAGCGGGCA CCAGTATCTG GGACCCAGAG AAAACAATTA GGCCTCGTGG ACGAGAGATC 1081 CAGCCTATAT GGGAAATAGA GCCAGTGAAG TGATGCTGTC CACCTCGGAT GGGGTCAGGC 1141 CTTCTTTTTG GCAACCTGG // |
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Primer: pAxEF_R2 Sequence file: RDB13256_A5D8c.seq
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>13256_13256_A5D8_2_pAxEF_R2_C05_08.ab1 1 CGGGTAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA 61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG 121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG 181 ATCAGTTATC TAGATCCGGT GGATCCCTAG AAGACAGGCA GCCTCGGGGA CGTGGTCAGC 241 GTGCAAGCAT TGATATCCTC AGTGTGGGCT GCCCGATGCA AGGATGGCTC GGCAGCGCTC 301 CGGTTGATCT TCGGTAGAGA GTGTTGGAGC AGCTCAATGG AAGACAGGAT CTGGGGAAAA 361 AGAGGCCTCT CTTCCTTTAC TTTCTTCACA CAGTCAGCTA CCAGCCTCTT CATTGCTTTG 421 GGGCAGTTCT TATATAGCTT ACTAAGATCT GGGGAGGCAT ATCCTCGGCC CACCATGAAG 481 ATGATCTGAT CTCGGTTGTT GATGTGAGAA TAAGGAAGCT CCCCCGTCAT CAGTTCATAC 541 AATACGATGC CATAGGAGTA GACATCCGAC TGGAAACTGA ATGGGTTGTT ATCCTGCATT 601 CGGATCACCT CTGGGGCCAT CCAGAGGACA GAGCCAGTAG GTTGTTCAAC CTGCTGAGAA 661 CCACTCCAGC GTGACTTTAC TGTTGCCAAA CCAAAATCTC CAATTTTCAC TGTTAAGCCT 721 TCATGGAGAA ATATATTGTT GGATTTCATG TCTCTATGGA TGATGTTCTT TGCATGCAAA 781 TAGTCCATTC CCTGAGCCGT CTGCCGGGCA ATGTCAATTA GCTGGAACAT CTGAAACTTG 841 GTCTCCTGGA CATGCAGGTG TTTGTAGAGG CTGCTGCCCT CGCACCACTG GGTCACAATT 901 GCCAGGTTGT CCTTTGTCAT GTACCCCATG AAAAGCAGAA TGTTCACATG CCGTGTTTTG 961 CGCAGAACAG CCACCTCATT CCTGAAGGCC TGGAATTTGC TCTGGGGGTT GGGTCGACAA 1021 CCCTTTAGGA TCTTTACTGC AACATCTCCG TGCCCATTTA CCCTTTATAA ACAGACCAAA 1081 GAGCCTGACC CACTCGAATG ACAGCCATCA CCTTCACTGC CTCCTATTTC CGAATATAGC 1141 TGCAATCTCT CTGCATCCGC AG // |
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Primer: SV40pro_F_V2 Sequence file: RDB13256_A5D8d.seq
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>13256_13256_A5D8_2_SV40proF_V2_A11_02.ab1 1 TAAGAGCTTT TTTGGGAGCC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC 61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG 841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG 901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC 961 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGGA 1021 GTTCTTCGCC ACCCTAGGGG AGCTAACTGA AACACGGAAG GAGACATACC GGGAGACCCG 1081 CGCTATGACG GCAATAAAGA CCGATTAAAC GCACGTGTGG GTCGGTTTGT TTCATAAACG 1141 GCCGGGGG // |
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Sequencing and PCR primers for primer information.
References
Original, user report and related articles
| original | Hibino, K., Activation kinetics of RAF protein in the ternary complex of RAF, RAS-GTP, and kinase on the plasma membrane of living cells: single-molecule imaging analysis. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573. [link to RRC of NBRP] |
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