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pSpCas9(BB)-2A-puro-ACAT2 sgRNA

Expression vector of sgRNA for targeting human ACAT2 and Cas9.

Catalog number RDB14698
Resource name pSpCas9(BB)-2A-puro-ACAT2 sgRNA
Clone info. Expression vector of sgRNA for targeting human acyl-coenzyme A: cholesterol acyltransferase type 2 (ACAT2), SpCAS9 and 2A-Puro.
Expression was confirmed by the depositor with fluorescence microscopy. Recommended host E. coli strain, stbl3.
Vector backbone PX459 (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human ACAT2 cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |
 
Other clones in our bank human ACAT2 (NCBI Gene 39) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14698 pSpCas9(BB)-2A-puro-ACAT2 sgRNA Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

2019.07.19

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