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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-Rab18 Q67L (#RDB14700)

Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.

Clone info. Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14700 pDEST/mCherry-Rab18 Q67L DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-Rab18 Q67L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14700).

Reference section:

Makino, A., Hullin-Matsuda, F., Murate, M., Abe, M., Tomishige, N., Fukuda, M., Yamashita, S., Fujimoto, T., Vidal, H., Lagarde, M., Delton, I., Kobayashi, T., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14700_A7Djp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F ()
Sequence file: RDB14700_A7Dja.seq check
>D04483A4_A7Dj_2_mCherry-C_F_E05_14_ABI24.ab1
    1 AACAAAAAGA AGCCCGGTGC AGCTGGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA
   61 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC
  121 ACTCCACCGG CGGCATGGAC GAGCTGTACA AGAAGCTTAC AAGTTTGTAC AAAAAAGCAG
  181 GCTCCGCGGC CGCCTTGTTT AACTTTAAGA AGGAGCCCTT CACCATGGAC GAGGACGTGC
  241 TAACCACCCT GAAGATCCTC ATCATCGGCG AGAGTGGGGT GGGCAAGTCC AGCCTGCTCT
  301 TGAGGTTCAC AGATGATACG TTTGATCCAG AACTTGCAGC AACAATAGGT GTTGACTTTA
  361 AGGTGAAAAC AATTTCAGTG GATGGAAATA AGGCTAAACT TGCAATATGG GATACTGCTG
  421 GTCTCGAGAG GTTTAGAACA TTAACTCCCA GCTATTATAG AGGTGCACAG GGTGTTATAT
  481 TAGTTTATGA TGTCACAAGA AGAGATACAT TTGTTAAACT GGATAATTGG TTAAATGAAT
  541 TGGAAACATA CTGTACAAGA AATGACATAG TAAACATGCT AGTTGGAAAT AAAATCGATA
  601 AGGAAAATCG TGAAGTCGAT AGAAATGAAG GCCTGAAATT TGCACGAAAG CATTCCATGT
  661 TATTTATAGA GGCAAGTGCA AAAACCTGTG ATGGTGTACA ATGTGCCTTT GAAGAACTTG
  721 TTGAAAAGAT CATTCAGACC CCTGGACTGT GGGAAAGTGA GAACCAGAAT AAAGGAGTCA
  781 AACTGTCACA CAGGGAAGAA GGCCAAGGAG GAGGAGCCTG TGGTGGTTAT TGCTCTGTGT
  841 TATAAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT CTAGAGGGCC
  901 CGCGGTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG
  961 GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTTC
 1021 TAGTTGGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGGC CTTTCCTTGA CCCTGGGAAG
 1081 GGTGGCCAAC TCCCCACTGT CCTTTCTAAT AAAATGAGGG AAATTGGCCA TTCGCATGGC
1141 TTGAGTAGGT GTCAATTCCT AATTCTGGGG ATGGAGTGGG CGAGGGAACA CAGGAC
//
Primer: BGH_rev2 ()
Sequence file: RDB14700_A7Djb.seq check
>D04483A4_A7Dj_2_BGH_rev2_F05_17_ABI24.ab1
    1 GATGCGTAAA GAATCAAGTC AAGCACGGGG ACAGGGCAGA CAACAGATGG CGTGGCAAGT
   61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC
  121 GGTACGCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG
  181 GCCCTCTAGA TCAACCACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC CCTTTTATAA
  241 CACAGAGCAA TAACCACCAC AGGCTCCTCC TCCTTGGCCT TCTTCCCTGT GTGACAGTTT
  301 GACTCCTTTA TTCTGGTTCT CACTTTCCCA CAGTCCAGGG GTCTGAATGA TCTTTTCAAC
  361 AAGTTCTTCA AAGGCACATT GTACACCATC ACAGGTTTTT GCACTTGCCT CTATAAATAA
  421 CATGGAATGC TTTCGTGCAA ATTTCAGGCC TTCATTTCTA TCGACTTCAC GATTTTCCTT
  481 ATCGATTTTA TTTCCAACTA GCATGTTTAC TATGTCATTT CTTGTACAGT ATGTTTCCAA
  541 TTCATTTAAC CAATTATCCA GTTTAACAAA TGTATCTCTT CTTGTGACAT CATAAACTAA
  601 TATAACACCC TGTGCACCTC TATAATAGCT GGGAGTTAAT GTTCTAAACC TCTCGAGACC
  661 AGCAGTATCC CATATTGCAA GTTTAGCCTT ATTTCCATCC ACTGAAATTG TTTTCACCTT
  721 AAAGTCAACA CCTATTGTTG CTGCAAGTTC TGGATCAAAC GTATCATCTG TGAACCTCAA
  781 GAGCAGGCTG GACTTGCCCA CCCCACTCTC GCCGATGATG AGGATCTTCA GGGTGGTTAG
  841 CACGTCCTCG TCCATGGTGA AGGGCTCCTT CTTAAAGTTA AACAAGGCGG CCGCGGAGCC
  901 TGCTTTTTTG TACAAACTTG TAAGCTTCTT GTACAGCTCG TCCATGCCGC CGGTGGGAGT
  961 GGCGGCCCTC GGCGCGTTCG TACTGGTTCC ACGATGGTGT AGTCCTCGTT TGTGGGAGGT
 1021 GATGTCCCAA CTTGATGTGA CGGTTGTAGC GCCGGGCAGC TGCACGGCTT TCTTGGCCTT
 1081 GTAGGTGATC CTGACTCAGC GTCGTTAGTG GACCGCGTCC TTCAGCTCAG GCATCTGGTT
1141 GAATCTCGCC CTTCCAGGAC GGCCCGGTTC TCTCGAGA
//
Primer: CMV-Forward ()
Sequence file: RDB14700_A7Djc.seq check
>D04483A4_A7Dj_2_CMV-Forward_G05_20_ABI24.ab1
    1 ACGTTCCAAC TTCCGGCCCC CAATTGGACG CAAATGGGCG GTAGGCGTTG TACGGTGGGA
   61 GGTCTATATA AGCAGAGCTC TTTTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
  121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT
  181 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
  241 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
  301 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
  361 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
  421 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
  481 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
  541 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
  601 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
  661 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
  721 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
  781 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
  841 CGCCGAGGGC CGCCACTCCA CCGGGCGGCA TGGACGAGCT GTACAAGAAG CTTACAAGTT
  901 TGTACAAAAA AAAGCAGGCT CCGCGGCCGC CTTGTTTAAC TTTAAGAAGG AGCCCTTCAC
  961 CATGGACGAG GACGTGCTAA CCACCCTGAA GATCCTCATC ATCGGCGAGA GTGGGGTGGG
 1021 GCAAGTTCCA GCCTGCTCTT GAGGGTTCAC AGATGATAAC GTTTGGATCC AGACTTGCAG
 1081 GCACCATAGG GTGGTTGACC TTTAAGGTGG AAACCATTTC AAGTGATGGG AATTAGGCTA
1141 ACACTGCAAT ATTGGGGAAT ACTGGTGCCT TGGTCTCTCT CCGCGAAG
//
Primer: SV40pro_F ()
Sequence file: RDB14700_A7Djd.seq check
>D04483A4_A7Dj_2_SV40pro_F_H05_23_ABI24.ab1
    1 CCCTTGGTCC TCGGAACATT CAGGAGTAGG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
   61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA
  121 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
  181 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
  241 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
  301 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
  361 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
  421 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
  481 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
  541 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
  601 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
  661 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
  721 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC
  781 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
  841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GGCCGCTCCC GATTCGCAGC GCATCGCCTT
  901 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC
  961 CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTTCC ACCGCCGCCT TCTATGAAAG
 1021 GTTGGGGCTT TCGGATCGTT TTCGGGACGC CGGCTGGATG ATCTCAGCGC GGGGATCTCA
 1081 TGCTGGAGTT CTTCGCCCAC CCAAACTGTA TGCCAGCTTA TATGTTACCA TTAAGCATAG
1141 CATCCAAGTG TCCACGCAAA GTATGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]