Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.
Clone info. | Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry. |
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Comment | Expression was confirmed by the depositor with immunofluorescence. |
Vector backbone | pDEST-mCherry (plasmid) |
Selectable markers | Amp^r (E. coli) |
Gene/insert name | human RAB18 cDNA |
Depositor|Developer | Sako, Yasushi | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016). |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14700 | pDEST/mCherry-Rab18 Q67L | DNA solution |
Materials & Methods section:
The pDEST/mCherry-Rab18 Q67L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14700). |
Reference section:
Makino, A., Hullin-Matsuda, F., Murate, M., Abe, M., Tomishige, N., Fukuda, M., Yamashita, S., Fujimoto, T., Vidal, H., Lagarde, M., Delton, I., Kobayashi, T., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14700_A7Djp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: mCherry-C_F () Sequence file: RDB14700_A7Dja.seq |
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>D04483A4_A7Dj_2_mCherry-C_F_E05_14_ABI24.ab1 1 AACAAAAAGA AGCCCGGTGC AGCTGGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA 61 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC 121 ACTCCACCGG CGGCATGGAC GAGCTGTACA AGAAGCTTAC AAGTTTGTAC AAAAAAGCAG 181 GCTCCGCGGC CGCCTTGTTT AACTTTAAGA AGGAGCCCTT CACCATGGAC GAGGACGTGC 241 TAACCACCCT GAAGATCCTC ATCATCGGCG AGAGTGGGGT GGGCAAGTCC AGCCTGCTCT 301 TGAGGTTCAC AGATGATACG TTTGATCCAG AACTTGCAGC AACAATAGGT GTTGACTTTA 361 AGGTGAAAAC AATTTCAGTG GATGGAAATA AGGCTAAACT TGCAATATGG GATACTGCTG 421 GTCTCGAGAG GTTTAGAACA TTAACTCCCA GCTATTATAG AGGTGCACAG GGTGTTATAT 481 TAGTTTATGA TGTCACAAGA AGAGATACAT TTGTTAAACT GGATAATTGG TTAAATGAAT 541 TGGAAACATA CTGTACAAGA AATGACATAG TAAACATGCT AGTTGGAAAT AAAATCGATA 601 AGGAAAATCG TGAAGTCGAT AGAAATGAAG GCCTGAAATT TGCACGAAAG CATTCCATGT 661 TATTTATAGA GGCAAGTGCA AAAACCTGTG ATGGTGTACA ATGTGCCTTT GAAGAACTTG 721 TTGAAAAGAT CATTCAGACC CCTGGACTGT GGGAAAGTGA GAACCAGAAT AAAGGAGTCA 781 AACTGTCACA CAGGGAAGAA GGCCAAGGAG GAGGAGCCTG TGGTGGTTAT TGCTCTGTGT 841 TATAAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT CTAGAGGGCC 901 CGCGGTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG 961 GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTTC 1021 TAGTTGGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGGC CTTTCCTTGA CCCTGGGAAG 1081 GGTGGCCAAC TCCCCACTGT CCTTTCTAAT AAAATGAGGG AAATTGGCCA TTCGCATGGC 1141 TTGAGTAGGT GTCAATTCCT AATTCTGGGG ATGGAGTGGG CGAGGGAACA CAGGAC // |
Primer: BGH_rev2 () Sequence file: RDB14700_A7Djb.seq |
>D04483A4_A7Dj_2_BGH_rev2_F05_17_ABI24.ab1 1 GATGCGTAAA GAATCAAGTC AAGCACGGGG ACAGGGCAGA CAACAGATGG CGTGGCAAGT 61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC 121 GGTACGCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG 181 GCCCTCTAGA TCAACCACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC CCTTTTATAA 241 CACAGAGCAA TAACCACCAC AGGCTCCTCC TCCTTGGCCT TCTTCCCTGT GTGACAGTTT 301 GACTCCTTTA TTCTGGTTCT CACTTTCCCA CAGTCCAGGG GTCTGAATGA TCTTTTCAAC 361 AAGTTCTTCA AAGGCACATT GTACACCATC ACAGGTTTTT GCACTTGCCT CTATAAATAA 421 CATGGAATGC TTTCGTGCAA ATTTCAGGCC TTCATTTCTA TCGACTTCAC GATTTTCCTT 481 ATCGATTTTA TTTCCAACTA GCATGTTTAC TATGTCATTT CTTGTACAGT ATGTTTCCAA 541 TTCATTTAAC CAATTATCCA GTTTAACAAA TGTATCTCTT CTTGTGACAT CATAAACTAA 601 TATAACACCC TGTGCACCTC TATAATAGCT GGGAGTTAAT GTTCTAAACC TCTCGAGACC 661 AGCAGTATCC CATATTGCAA GTTTAGCCTT ATTTCCATCC ACTGAAATTG TTTTCACCTT 721 AAAGTCAACA CCTATTGTTG CTGCAAGTTC TGGATCAAAC GTATCATCTG TGAACCTCAA 781 GAGCAGGCTG GACTTGCCCA CCCCACTCTC GCCGATGATG AGGATCTTCA GGGTGGTTAG 841 CACGTCCTCG TCCATGGTGA AGGGCTCCTT CTTAAAGTTA AACAAGGCGG CCGCGGAGCC 901 TGCTTTTTTG TACAAACTTG TAAGCTTCTT GTACAGCTCG TCCATGCCGC CGGTGGGAGT 961 GGCGGCCCTC GGCGCGTTCG TACTGGTTCC ACGATGGTGT AGTCCTCGTT TGTGGGAGGT 1021 GATGTCCCAA CTTGATGTGA CGGTTGTAGC GCCGGGCAGC TGCACGGCTT TCTTGGCCTT 1081 GTAGGTGATC CTGACTCAGC GTCGTTAGTG GACCGCGTCC TTCAGCTCAG GCATCTGGTT 1141 GAATCTCGCC CTTCCAGGAC GGCCCGGTTC TCTCGAGA // |
Primer: CMV-Forward () Sequence file: RDB14700_A7Djc.seq |
>D04483A4_A7Dj_2_CMV-Forward_G05_20_ABI24.ab1 1 ACGTTCCAAC TTCCGGCCCC CAATTGGACG CAAATGGGCG GTAGGCGTTG TACGGTGGGA 61 GGTCTATATA AGCAGAGCTC TTTTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG 121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT 181 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT 241 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG 301 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT 361 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC 421 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT 481 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG 541 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT 601 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC 661 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA 721 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA 781 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG 841 CGCCGAGGGC CGCCACTCCA CCGGGCGGCA TGGACGAGCT GTACAAGAAG CTTACAAGTT 901 TGTACAAAAA AAAGCAGGCT CCGCGGCCGC CTTGTTTAAC TTTAAGAAGG AGCCCTTCAC 961 CATGGACGAG GACGTGCTAA CCACCCTGAA GATCCTCATC ATCGGCGAGA GTGGGGTGGG 1021 GCAAGTTCCA GCCTGCTCTT GAGGGTTCAC AGATGATAAC GTTTGGATCC AGACTTGCAG 1081 GCACCATAGG GTGGTTGACC TTTAAGGTGG AAACCATTTC AAGTGATGGG AATTAGGCTA 1141 ACACTGCAAT ATTGGGGAAT ACTGGTGCCT TGGTCTCTCT CCGCGAAG // |
Primer: SV40pro_F () Sequence file: RDB14700_A7Djd.seq |
>D04483A4_A7Dj_2_SV40pro_F_H05_23_ABI24.ab1 1 CCCTTGGTCC TCGGAACATT CAGGAGTAGG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 121 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 181 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 241 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 301 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 361 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 421 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 481 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 541 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 601 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 661 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 721 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 781 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GGCCGCTCCC GATTCGCAGC GCATCGCCTT 901 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC 961 CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTTCC ACCGCCGCCT TCTATGAAAG 1021 GTTGGGGCTT TCGGATCGTT TTCGGGACGC CGGCTGGATG ATCTCAGCGC GGGGATCTCA 1081 TGCTGGAGTT CTTCGCCCAC CCAAACTGTA TGCCAGCTTA TATGTTACCA TTAAGCATAG 1141 CATCCAAGTG TCCACGCAAA GTATGA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP] |
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