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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-Rab18 Q67L

Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.

Catalog number RDB14700
Resource name pDEST/mCherry-Rab18 Q67L
Clone info. Expression vector of human Rab18, constitutively active GTP-binding mutant (Q67L), fused with mCherry.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
Other clones in our bank

External Database
human RAB18

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14700 pDEST/mCherry-Rab18 Q67L DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14700_A7Djp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mCherry-C_F
          Sequence file: RDB14700_A7Dja.seq check
          >D04483A4_A7Dj_2_mCherry-C_F_E05_14_ABI24.ab1
              1 AACAAAAAGA AGCCCGGTGC AGCTGGCCCG GCGCCTACAA CGTCAACATC AAGTTGGACA
             61 TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGCGCC GAGGGCCGCC
            121 ACTCCACCGG CGGCATGGAC GAGCTGTACA AGAAGCTTAC AAGTTTGTAC AAAAAAGCAG
            181 GCTCCGCGGC CGCCTTGTTT AACTTTAAGA AGGAGCCCTT CACCATGGAC GAGGACGTGC
            241 TAACCACCCT GAAGATCCTC ATCATCGGCG AGAGTGGGGT GGGCAAGTCC AGCCTGCTCT
            301 TGAGGTTCAC AGATGATACG TTTGATCCAG AACTTGCAGC AACAATAGGT GTTGACTTTA
            361 AGGTGAAAAC AATTTCAGTG GATGGAAATA AGGCTAAACT TGCAATATGG GATACTGCTG
            421 GTCTCGAGAG GTTTAGAACA TTAACTCCCA GCTATTATAG AGGTGCACAG GGTGTTATAT
            481 TAGTTTATGA TGTCACAAGA AGAGATACAT TTGTTAAACT GGATAATTGG TTAAATGAAT
            541 TGGAAACATA CTGTACAAGA AATGACATAG TAAACATGCT AGTTGGAAAT AAAATCGATA
            601 AGGAAAATCG TGAAGTCGAT AGAAATGAAG GCCTGAAATT TGCACGAAAG CATTCCATGT
            661 TATTTATAGA GGCAAGTGCA AAAACCTGTG ATGGTGTACA ATGTGCCTTT GAAGAACTTG
            721 TTGAAAAGAT CATTCAGACC CCTGGACTGT GGGAAAGTGA GAACCAGAAT AAAGGAGTCA
            781 AACTGTCACA CAGGGAAGAA GGCCAAGGAG GAGGAGCCTG TGGTGGTTAT TGCTCTGTGT
            841 TATAAAAGGG TGGGCGCGCC GACCCAGCTT TCTTGTACAA AGTGGTTGAT CTAGAGGGCC
            901 CGCGGTTCGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGCGTACCG
            961 GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTTC
           1021 TAGTTGGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGGC CTTTCCTTGA CCCTGGGAAG
           1081 GGTGGCCAAC TCCCCACTGT CCTTTCTAAT AAAATGAGGG AAATTGGCCA TTCGCATGGC
          1141 TTGAGTAGGT GTCAATTCCT AATTCTGGGG ATGGAGTGGG CGAGGGAACA CAGGAC
          //
          Primer: BGH_rev2
          Sequence file: RDB14700_A7Djb.seq check
          >D04483A4_A7Dj_2_BGH_rev2_F05_17_ABI24.ab1
              1 GATGCGTAAA GAATCAAGTC AAGCACGGGG ACAGGGCAGA CAACAGATGG CGTGGCAAGT
             61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC
            121 GGTACGCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG
            181 GCCCTCTAGA TCAACCACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC CCTTTTATAA
            241 CACAGAGCAA TAACCACCAC AGGCTCCTCC TCCTTGGCCT TCTTCCCTGT GTGACAGTTT
            301 GACTCCTTTA TTCTGGTTCT CACTTTCCCA CAGTCCAGGG GTCTGAATGA TCTTTTCAAC
            361 AAGTTCTTCA AAGGCACATT GTACACCATC ACAGGTTTTT GCACTTGCCT CTATAAATAA
            421 CATGGAATGC TTTCGTGCAA ATTTCAGGCC TTCATTTCTA TCGACTTCAC GATTTTCCTT
            481 ATCGATTTTA TTTCCAACTA GCATGTTTAC TATGTCATTT CTTGTACAGT ATGTTTCCAA
            541 TTCATTTAAC CAATTATCCA GTTTAACAAA TGTATCTCTT CTTGTGACAT CATAAACTAA
            601 TATAACACCC TGTGCACCTC TATAATAGCT GGGAGTTAAT GTTCTAAACC TCTCGAGACC
            661 AGCAGTATCC CATATTGCAA GTTTAGCCTT ATTTCCATCC ACTGAAATTG TTTTCACCTT
            721 AAAGTCAACA CCTATTGTTG CTGCAAGTTC TGGATCAAAC GTATCATCTG TGAACCTCAA
            781 GAGCAGGCTG GACTTGCCCA CCCCACTCTC GCCGATGATG AGGATCTTCA GGGTGGTTAG
            841 CACGTCCTCG TCCATGGTGA AGGGCTCCTT CTTAAAGTTA AACAAGGCGG CCGCGGAGCC
            901 TGCTTTTTTG TACAAACTTG TAAGCTTCTT GTACAGCTCG TCCATGCCGC CGGTGGGAGT
            961 GGCGGCCCTC GGCGCGTTCG TACTGGTTCC ACGATGGTGT AGTCCTCGTT TGTGGGAGGT
           1021 GATGTCCCAA CTTGATGTGA CGGTTGTAGC GCCGGGCAGC TGCACGGCTT TCTTGGCCTT
           1081 GTAGGTGATC CTGACTCAGC GTCGTTAGTG GACCGCGTCC TTCAGCTCAG GCATCTGGTT
          1141 GAATCTCGCC CTTCCAGGAC GGCCCGGTTC TCTCGAGA
          //
          Primer: CMV-Forward
          Sequence file: RDB14700_A7Djc.seq check
          >D04483A4_A7Dj_2_CMV-Forward_G05_20_ABI24.ab1
              1 ACGTTCCAAC TTCCGGCCCC CAATTGGACG CAAATGGGCG GTAGGCGTTG TACGGTGGGA
             61 GGTCTATATA AGCAGAGCTC TTTTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
            121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT
            181 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
            241 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
            301 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
            361 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
            421 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
            481 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
            541 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
            601 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
            661 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
            721 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
            781 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
            841 CGCCGAGGGC CGCCACTCCA CCGGGCGGCA TGGACGAGCT GTACAAGAAG CTTACAAGTT
            901 TGTACAAAAA AAAGCAGGCT CCGCGGCCGC CTTGTTTAAC TTTAAGAAGG AGCCCTTCAC
            961 CATGGACGAG GACGTGCTAA CCACCCTGAA GATCCTCATC ATCGGCGAGA GTGGGGTGGG
           1021 GCAAGTTCCA GCCTGCTCTT GAGGGTTCAC AGATGATAAC GTTTGGATCC AGACTTGCAG
           1081 GCACCATAGG GTGGTTGACC TTTAAGGTGG AAACCATTTC AAGTGATGGG AATTAGGCTA
          1141 ACACTGCAAT ATTGGGGAAT ACTGGTGCCT TGGTCTCTCT CCGCGAAG
          //
          Primer: SV40pro_F
          Sequence file: RDB14700_A7Djd.seq check
          >D04483A4_A7Dj_2_SV40pro_F_H05_23_ABI24.ab1
              1 CCCTTGGTCC TCGGAACATT CAGGAGTAGG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
             61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA
            121 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
            181 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
            241 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
            301 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
            361 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
            421 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
            481 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
            541 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
            601 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
            661 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
            721 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC
            781 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
            841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GGCCGCTCCC GATTCGCAGC GCATCGCCTT
            901 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC
            961 CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTTCC ACCGCCGCCT TCTATGAAAG
           1021 GTTGGGGCTT TCGGATCGTT TTCGGGACGC CGGCTGGATG ATCTCAGCGC GGGGATCTCA
           1081 TGCTGGAGTT CTTCGCCCAC CCAAACTGTA TGCCAGCTTA TATGTTACCA TTAAGCATAG
          1141 CATCCAAGTG TCCACGCAAA GTATGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl