Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pACYC184-Venus-MEK1 (S218/222E, delta32-51) (#RDB14154)

Plasmid clone of constitutive active form of human MEK1.

Alternative name GL 571
Clone info. Plasmid clone of constitutive active form of human MEK1.
Comment Constitutive active form of human MEK1(S218E, S222E and delta 32-35) fused to Venus with 8Gly linker is introduced into pACYC184 vector. Expression was confirmed by the depositor.
Vector backbone pACYC184 (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Cm^r, Tet^r.
Gene/insert name human MEK1 cDNA
Depositor|Developer Sako, Yasushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14154 pACYC184-Venus-MEK1 (S218/222E, delta32-51) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pACYC184-Venus-MEK1 (S218/222E, delta32-51) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14154).

Reference section:

Shindo, Y., Iwamoto, K., Mouri, K., Hibino, K., Tomita, M., Kosako, H., Sako, Y., Takahashi, K., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14154_A6Ecp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP_N_rev
Sequence file: RDB14154_A6Eca.seq check
>D03441A2_A6Ec_3_EGFP-N_rev_A11_01_ABI08.ab1
    1 AGAATTAAAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
   61 CCCTGAAGCT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  121 CCCTCGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
  181 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  241 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  301 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  361 ACAACTACAA CAGCCACAAC GTCTATATCA CAGCCGACAA GCAGAAGAAC GGCATCAAGG
  421 CAAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGTGT GCAGCTCGCC GACCACTACC
  481 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT
  541 ACCAGTCCAA ACTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  601 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT GGCGGTGGCG
  661 GTGGCGGTGG CCTCGACCCC AAGAAGAAGC CGACGCCCAT CCAGCTGAAC CCGGCCCCCG
  721 ACGGCTCTGC AGTTAACGGG ACCAGCTCTG CGGAGACCAA CTTGGAGGCC TTTCTTACCC
  781 AGAAGCAGAA GTGGGAGAAC TGAAGATGAC GACTTTGAGA AGATCAGTGA GCTGGGGGGC
  841 TGGCAATGGC CGGTGTGGTG TTCAAAGTCT CCCACAAGCC TCTGGGCCTG GTCATGGCCA
  901 GAAAAGCTAA TTCATCTGGA AGATCAAACC CGGCAATCCG AACCAGATCA TAAGGAGGCT
  961 GGCAAGTCTG CATGGAGTGC CACCTCTCCG TACATTCGTG GGCTTCTATG GGTGCGTTCT
 1021 ACAGCGATGG CGGAGAATCA AGTATTCTTG GCATTGGGAG CCATTGATGA AGTCCTCTGG
1081 AAGTCAGTCC TGAGCAGCTG AAAT
//
Primer: EGFP-C
Sequence file: RDB14154_A6Ecb.seq check
>D03441A2_A6Ec_3_EGFP_C_B11_02_ABI08.ab1
    1 CCGGGAATAT CTCGGGCATG GACGAGCTGT ACAAGGGTGG CGGTGGCGGT GGCGGTGGCC
   61 TCGACCCCAA GAAGAAGCCG ACGCCCATCC AGCTGAACCC GGCCCCCGAC GGCTCTGCAG
  121 TTAACGGGAC CAGCTCTGCG GAGACCAACT TGGAGGCCTT TCTTACCCAG AAGCAGAAGG
  181 TGGGAGAACT GAAGGATGAC GACTTTGAGA AGATCAGTGA GCTGGGGGCT GGCAATGGCG
  241 GTGTGGTGTT CAAGGTCTCC CACAAGCCTT CTGGCCTGGT CATGGCCAGA AAGCTAATTC
  301 ATCTGGAGAT CAAACCCGCA ATCCGGAACC AGATCATAAG GGAGCTGCAG GTTCTGCATG
  361 AGTGCAACTC TCCGTACATC GTGGGCTTCT ATGGTGCGTT CTACAGCGAT GGCGAGATCA
  421 GTATCTGCAT GGAGCACATG GATGGAGGTT CTCTGGATCA AGTCCTGAAG AAAGCTGGAA
  481 GAATTCCTGA ACAAATTTTA GGAAAAGTTA GCATTGCTGT AATAAAAGGC CTGACATATC
  541 TGAGGGAGAA GCACAAGATC ATGCACAGAG ATGTCAAGCC CTCCAACATC CTAGTCAACT
  601 CCCGTGGGGA GATCAAGCTC TGTGACTTTG GGGTCAGCGG GCAGCTCATC GACGAAATGG
  661 CCAACGAATT CGTGGGCACA AGGTCCTACA TGTCGCCAGA AAGACTCCAG GGGACTCATT
  721 ACTCTGTGCA GTCAGACATC TGGAGCATGG GACTGTCTCT GGTAGAGATG GCGGTTGGGA
  781 GGTATCCCAT CCCTCCTCCA GATGCCAAGA GCTGGAGCTG ATGTTTGGGT GCCAGTGGAA
  841 GGAGATGCGG CTGAGACCCC ACCAGGCCAA GGACCCCCGG GAGGCCCTTA GCTCATACGG
  901 AATGGACAGC CGACCTCCCA TGGCAATTTT TGAGTTGTTG ATTACATAGT CACGAGCTCT
  961 TCGAAAACTG CCCAGTGGGA GTGTCAGTCT GCATTTCAGG ATTTTTGTGA ATAATTGCCT
 1021 AATAAAACCC GCAGAGGAGC GAATTGAAGC AACTCATGTC ATGCTTATCA GAGATTCGAT
1081 GCCTGAGGGA A
//
Primer: MEK-f2
Sequence file: RDB14154_A6Ecc.seq check
>D03441A2_A6Ec_3_MEK-f2_C11_03_ABI08.ab1
    1 TCAGAAAGAG TCAAGCCCTC CAACATCCTA GTCAACTCCC GTGGGGAGAT CAAGCTCTGT
   61 GACTTTGGGG TCAGCGGGCA GCTCATCGAC GAAATGGCCA ACGAATTCGT GGGCACAAGG
  121 TCCTACATGT CGCCAGAAAG ACTCCAGGGG ACTCATTACT CTGTGCAGTC AGACATCTGG
  181 AGCATGGGAC TGTCTCTGGT AGAGATGGCG GTTGGGAGGT ATCCCATCCC TCCTCCAGAT
  241 GCCAAGGAGC TGGAGCTGAT GTTTGGGTGC CAGGTGGAAG GAGATGCGGC TGAGACCCCA
  301 CCCAGGCCAA GGACCCCCGG GAGGCCCCTT AGCTCATACG GAATGGACAG CCGACCTCCC
  361 ATGGCAATTT TTGAGTTGTT GGATTACATA GTCAACGAGC CTCCTCCAAA ACTGCCCAGT
  421 GGAGTGTTCA GTCTGGAATT TCAAGATTTT GTGAATAAAT GCTTAATAAA AAACCCCGCA
  481 GAGAGAGCAG ATTTGAAGCA ACTCATGGTT CATGCTTTTA TCAAGAGATC CGATGCTGAG
  541 GAAGTGGATT TTGCAGGTTG GCTCTGCTCC ACCATCGGCC TTAACCAGCC CAGCACACCA
  601 ACCCATGCTG CTGGCGTCTA AACTAGTCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA
  661 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA
  721 TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGACGC
  781 CCGCCCATAA ACTGCCAGGC ATCAAATTAA GCAGAACGCC ATCCTGACGG ATGGCCTTTT
  841 TTGCGTTTCT ACAACTCTTC TGTCGTCATA TCTAACGGCC GACGCGCCTG GCTACGTCTT
  901 GCTGGCGTTT CCGCGACGCG AAGCTGAATG CCTTCCCCAT ATGAATTCTT CCTCGCTTCC
  961 GGCGGCATCG GAATTGCCCG CCGTGCCAGG CAATTGCTGT TCCAGGCCAG TTAGAATGGA
 1021 CGACCATCCA GGAACGCTCA GAATCGCCTC CCGCTTACAG CTAACATTCG GATCCATGGA
1081 GAACGGCGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP]