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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pACYC184-Venus-MEK1 (S218/222E, delta32-51)

Plasmid clone of constitutive active form of human MEK1.

Catalog number RDB14154
Resource name pACYC184-Venus-MEK1 (S218/222E, delta32-51)
Alternative name GL 571
Clone info. Plasmid clone of constitutive active form of human MEK1.
Comment Constitutive active form of human MEK1(S218E, S222E and delta 32-35) fused to Venus with 8Gly linker is introduced into pACYC184 vector. Expression was confirmed by the depositor.
Vector backbone pACYC184 (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Cm^r, Tet^r.
Gene/insert name human MEK1 cDNA
Depositor|Developer Sako, Yasushi |
Other clones in our bank

External Database
human MEK1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
          Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。
          付加的提供条件 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。非営利機関の非営利学術研究に限って提供する。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14154 pACYC184-Venus-MEK1 (S218/222E, delta32-51) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14154_A6Ecp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP_N_rev
          Sequence file: RDB14154_A6Eca.seq check
          >D03441A2_A6Ec_3_EGFP-N_rev_A11_01_ABI08.ab1
              1 AGAATTAAAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
             61 CCCTGAAGCT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
            121 CCCTCGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
            181 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
            241 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
            301 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
            361 ACAACTACAA CAGCCACAAC GTCTATATCA CAGCCGACAA GCAGAAGAAC GGCATCAAGG
            421 CAAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGTGT GCAGCTCGCC GACCACTACC
            481 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT
            541 ACCAGTCCAA ACTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
            601 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT GGCGGTGGCG
            661 GTGGCGGTGG CCTCGACCCC AAGAAGAAGC CGACGCCCAT CCAGCTGAAC CCGGCCCCCG
            721 ACGGCTCTGC AGTTAACGGG ACCAGCTCTG CGGAGACCAA CTTGGAGGCC TTTCTTACCC
            781 AGAAGCAGAA GTGGGAGAAC TGAAGATGAC GACTTTGAGA AGATCAGTGA GCTGGGGGGC
            841 TGGCAATGGC CGGTGTGGTG TTCAAAGTCT CCCACAAGCC TCTGGGCCTG GTCATGGCCA
            901 GAAAAGCTAA TTCATCTGGA AGATCAAACC CGGCAATCCG AACCAGATCA TAAGGAGGCT
            961 GGCAAGTCTG CATGGAGTGC CACCTCTCCG TACATTCGTG GGCTTCTATG GGTGCGTTCT
           1021 ACAGCGATGG CGGAGAATCA AGTATTCTTG GCATTGGGAG CCATTGATGA AGTCCTCTGG
          1081 AAGTCAGTCC TGAGCAGCTG AAAT
          //
          Primer: EGFP-C
          Sequence file: RDB14154_A6Ecb.seq check
          >D03441A2_A6Ec_3_EGFP_C_B11_02_ABI08.ab1
              1 CCGGGAATAT CTCGGGCATG GACGAGCTGT ACAAGGGTGG CGGTGGCGGT GGCGGTGGCC
             61 TCGACCCCAA GAAGAAGCCG ACGCCCATCC AGCTGAACCC GGCCCCCGAC GGCTCTGCAG
            121 TTAACGGGAC CAGCTCTGCG GAGACCAACT TGGAGGCCTT TCTTACCCAG AAGCAGAAGG
            181 TGGGAGAACT GAAGGATGAC GACTTTGAGA AGATCAGTGA GCTGGGGGCT GGCAATGGCG
            241 GTGTGGTGTT CAAGGTCTCC CACAAGCCTT CTGGCCTGGT CATGGCCAGA AAGCTAATTC
            301 ATCTGGAGAT CAAACCCGCA ATCCGGAACC AGATCATAAG GGAGCTGCAG GTTCTGCATG
            361 AGTGCAACTC TCCGTACATC GTGGGCTTCT ATGGTGCGTT CTACAGCGAT GGCGAGATCA
            421 GTATCTGCAT GGAGCACATG GATGGAGGTT CTCTGGATCA AGTCCTGAAG AAAGCTGGAA
            481 GAATTCCTGA ACAAATTTTA GGAAAAGTTA GCATTGCTGT AATAAAAGGC CTGACATATC
            541 TGAGGGAGAA GCACAAGATC ATGCACAGAG ATGTCAAGCC CTCCAACATC CTAGTCAACT
            601 CCCGTGGGGA GATCAAGCTC TGTGACTTTG GGGTCAGCGG GCAGCTCATC GACGAAATGG
            661 CCAACGAATT CGTGGGCACA AGGTCCTACA TGTCGCCAGA AAGACTCCAG GGGACTCATT
            721 ACTCTGTGCA GTCAGACATC TGGAGCATGG GACTGTCTCT GGTAGAGATG GCGGTTGGGA
            781 GGTATCCCAT CCCTCCTCCA GATGCCAAGA GCTGGAGCTG ATGTTTGGGT GCCAGTGGAA
            841 GGAGATGCGG CTGAGACCCC ACCAGGCCAA GGACCCCCGG GAGGCCCTTA GCTCATACGG
            901 AATGGACAGC CGACCTCCCA TGGCAATTTT TGAGTTGTTG ATTACATAGT CACGAGCTCT
            961 TCGAAAACTG CCCAGTGGGA GTGTCAGTCT GCATTTCAGG ATTTTTGTGA ATAATTGCCT
           1021 AATAAAACCC GCAGAGGAGC GAATTGAAGC AACTCATGTC ATGCTTATCA GAGATTCGAT
          1081 GCCTGAGGGA A
          //
          Primer: MEK-f2
          Sequence file: RDB14154_A6Ecc.seq check
          >D03441A2_A6Ec_3_MEK-f2_C11_03_ABI08.ab1
              1 TCAGAAAGAG TCAAGCCCTC CAACATCCTA GTCAACTCCC GTGGGGAGAT CAAGCTCTGT
             61 GACTTTGGGG TCAGCGGGCA GCTCATCGAC GAAATGGCCA ACGAATTCGT GGGCACAAGG
            121 TCCTACATGT CGCCAGAAAG ACTCCAGGGG ACTCATTACT CTGTGCAGTC AGACATCTGG
            181 AGCATGGGAC TGTCTCTGGT AGAGATGGCG GTTGGGAGGT ATCCCATCCC TCCTCCAGAT
            241 GCCAAGGAGC TGGAGCTGAT GTTTGGGTGC CAGGTGGAAG GAGATGCGGC TGAGACCCCA
            301 CCCAGGCCAA GGACCCCCGG GAGGCCCCTT AGCTCATACG GAATGGACAG CCGACCTCCC
            361 ATGGCAATTT TTGAGTTGTT GGATTACATA GTCAACGAGC CTCCTCCAAA ACTGCCCAGT
            421 GGAGTGTTCA GTCTGGAATT TCAAGATTTT GTGAATAAAT GCTTAATAAA AAACCCCGCA
            481 GAGAGAGCAG ATTTGAAGCA ACTCATGGTT CATGCTTTTA TCAAGAGATC CGATGCTGAG
            541 GAAGTGGATT TTGCAGGTTG GCTCTGCTCC ACCATCGGCC TTAACCAGCC CAGCACACCA
            601 ACCCATGCTG CTGGCGTCTA AACTAGTCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA
            661 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA
            721 TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGACGC
            781 CCGCCCATAA ACTGCCAGGC ATCAAATTAA GCAGAACGCC ATCCTGACGG ATGGCCTTTT
            841 TTGCGTTTCT ACAACTCTTC TGTCGTCATA TCTAACGGCC GACGCGCCTG GCTACGTCTT
            901 GCTGGCGTTT CCGCGACGCG AAGCTGAATG CCTTCCCCAT ATGAATTCTT CCTCGCTTCC
            961 GGCGGCATCG GAATTGCCCG CCGTGCCAGG CAATTGCTGT TCCAGGCCAG TTAGAATGGA
           1021 CGACCATCCA GGAACGCTCA GAATCGCCTC CCGCTTACAG CTAACATTCG GATCCATGGA
          1081 GAACGGCGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl