Resource data sheet
Prev.

pET28/His6-mCherry-D4

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens.

Catalog number RDB14300
Resource name pET28/His6-mCherry-D4
Clone info. Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 (domain 4 of theta toxin; K363-N472) of C. perfringens, see Fig. 1A of Shimada, Y. et. al. Eur. J. Biochem, 269: 6195-6203, 2002.
Expression was confirmed by the depositor with SDS-PAGE. DQ673099.1 for Clostridium perfringens CN3947 perfringolysin O (pfoA) gene.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Clostridium perfringens perfringolysin O (pfoA) cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A. et al., FASEB J. 2016 Aug 4. pii: fj.201500075R.)
Ordering Please visit Information of Request for Distribution. Please contact us. UCSD's MTA is also required.
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Remarks
Please contact us. UCSD's MTA is also required.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14300 pET28/His6-mCherry-D4 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Dnaconda's recommendation EXR0062e (English text)
Featured content Dnaconda's recommendation EXR0062j (Japanese text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14300_A6Hup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB14300_A6Hua.seq
>D03762A1_A6Hu_3_T7_20bp_A02_01_ABI08.ab1
    1 TAGAGGGTTC AAATTCCCCC TCTAGAATAA TTTTGTTTAA CTTTAAGGAA GGAGATATAC
   61 CATGGGCAGC AGCCATCATC ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCGG
  121 TACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT
  181 CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA
  241 GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT
  301 GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA
  361 GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA
  421 GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA
  481 GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC
  541 CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA
  601 CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA
  661 CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA
  721 CGTCAACATC AAGTTGGACA TCACCTCCCA CAAACGAGGA CTACACCATC GTGGAACAGT
  781 ACGAACGCGC CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGAAGCTTA
  841 AGGGAAAAAT AAACTTAGAT CATAGTGGAG CCTATGTTGC ACAGTTTGAA GTAGCCTGGG
  901 GATGAAGTTT CATATGACAA AGAAGAAATG AAGTTTTAAC TCATAAACAT GGGATGGAAA
  961 TTATCAAGAT AAAACAGCTC ACTATTTCAA CAGTAATACC TCTTGAAGCT AATGCAAGGA
 1021 ATATAAGAAT AAAAGCAAGA GAGTGTACAG GCCCTTGCTT TGGAAATGGG TGGAGAGATG
 1081 TAATAAGTGA ATATGATGTT CCATTTACAA TAATATAATG GTTCATATGG GAACAAACTT
1141 TATACCCTGG GGATTCCTAG TTAATTAACC TTAATCCA
//
Primer: T7terminater RDB14300_A6Hub.seq
>D03762A1_A6Hu_3_T7 Terminator_B02_02_ABI08.ab1
    1 GCTCCAAGCT TCCTTTTCGG GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG
   61 GTGCTCGAGT TAATTGTAAG TAATACTAGA TCCAGGGTAT AAAGTTGTTC CCCATATTGA
  121 AACATTTATA TTATTTGTTA ATGGAACATC ATATTCACTT ATAACATCTC TCCACCATTC
  181 CCAAGCAAGG CCTGTACACT CTCTTGCTTT TATTCTTATA TTTCTTGCAT TAGCTTCAAG
  241 AGGTATTACT GTTGAATAGT GAGCTGTTTT ATCTTGATAA TTTCCATCCC ATGTTTTATG
  301 AGTTAAAACT TCATTTCCTT CTTTGTCATA TGAAACTTCA TCCCAGGCTA CTTCAAACTG
  361 TGCAACATAG GCTCCACTAT GATCTAAGTT TATTTTTCCC TTAAGCTTCT TGTACAGCTC
  421 GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA
  481 GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC CGGGCAGCTG
  541 CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT
  601 CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC
  661 GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT
  721 GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT
  781 CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG
  841 GAAGGACAGC TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGGAGC
  901 CGTACATGAA CTGAGGGGGA CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC
  961 ACCTTCAGCT TTGCGGTCTG GGTGCCCTCG TAGGGCGGCC CTCGCCCTCG CCCTCGATCT
 1021 CGAACTCGTG GCCGTTCACG GAGCCTCCAA TGTGCACCTT TGAAGCGCAT GAACTCTTTG
 1081 ATGAATGGCC AATGTTATCC TCTCGACCCT TGCCTCACAA TGTACGCTGC GCGCGCACCA
1141 GTCGGCTGCT GGTGAATGAT GAATGAATGA AATGGGGCCG TGCG
//
Primer: mCherry-N_rev RDB14300_A6Huc.seq
>D03762A1_A6Hu_3_mCherry-N_rev_A02_02_ABI24.ab1
    1 GGGGCAATAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG CCCTTGCTCA CCATGGTACC
   61 GCTGCCGCGC GGCACCAGGC CGCTGCTGTG ATGATGATGA TGATGGCTGC TGCCCATGGT
  121 ATATCTCCTT CTTAAAGTTA AACAAAATTA TTTCTAGAGG GGAATTGTTA TCCGCTCACA
  181 ATTCCCCTAT AGTGAGTCGT ATTAATTTCG CGGGATCGAG ATCTCGATCC TCTACGCCGG
  241 ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA
  301 CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT
  361 GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC
  421 ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA
  481 GGAGTCGCAT AAGGGAGAGC GTCGAGATCC CGGACACCAT CGAATGGCGC AAAACCTTTC
  541 GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG
  601 TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG
  661 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG GCGATGGCGG
  721 AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC GGGCAAACAG TCGTTGCTGA
  781 TTGGCGTTGC CACATCCAGT CTGGCCCTGC ACGCGCCGTC GCAAATTGTC GCGGCGATTA
  841 AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG
  901 TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA
  961 TTAACTATCC GCTGGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT
 1021 CCCGGCGTTA TTTCTTTGAT GTCTCTGACC AGACACCCCA TCAACAGTAT TAATTTTCTC
 1081 CCATGAAGAC GGTACGCGAA CTGGGCCGTG AGCCATCCTG GATTCCGCAA TTGAGATCCA
1141 CCCAAGCCAA AAATTCGCGC GCGCCCGTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925.

2019.06.17

GNP_filter3_RDBDEP_html_190430.pl