Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pET28/His6-mCherry-D4 (#RDB14300)

Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens.

Clone info. Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 (domain 4 of theta toxin; K363-N472) of C. perfringens, see Fig. 1A of Shimada, Y. et. al. Eur. J. Biochem, 269: 6195-6203, 2002.
Comment Expression was confirmed by the depositor with SDS-PAGE. DQ673099.1 for Clostridium perfringens CN3947 perfringolysin O (pfoA) gene.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Clostridium perfringens perfringolysin O (pfoA) cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 
Sequence (full) RDB14300hts01.seq checkAssembled from experimentally sequenced data.
Publication Makino, A., FASEB J. 31 (4): 1301-1322 (2017). [link to RRC of NBRP]
Test sheet RDB14300_A9B1p1-4.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14300 pET28/His6-mCherry-D4 DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/His6-mCherry-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14300).

Reference section:

Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.G., Kasai, R.S., Kubo, S., Schieber, N.L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton R.G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [link to RRC of NBRP]
reference Abe, M., Imaging cholesterol depletion at the plasma membrane by methyl-β-cyclodextrin. J. Lipid Res. 62:100077 (2021). PMID 33891936.
user_report Miyata, Y., Protocol to analyze lipid asymmetry in the plasma membrane. STAR Protoc. 3 (4): 101870 (2022). PMID 36595929. [link to RRC of NBRP]
user_report Kitano, V.J.F., LDL uptake-dependent phosphatidylethanolamine translocation to the cell surface promotes fusion of osteoclast-like cells. J. Cell Sci. 133 (10): jcs243840 (2020). PMID 32295848. [link to RRC of NBRP]