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GL023

Expression plasmid of GFP-tagged human c-RAF.

Catalog number RDB13254
Resource name GL023
Clone info. Expression plasmid of GFP-tagged human c-RAF, GFP-RAF in Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.
Comment Expression was confirmed by the depositor with immunoblotting and live-cell imaging.
Vector backbone pEGFP-C2 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human c-raf1 cDNA
Depositor|Developer Sako, Yasushi |
Other clones in our bank

External Database
Human c-raf1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Biophys. J. 97, 1277-1287, 2009)
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biophys. J. 97, 1277-1287, 2009)。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13254 GL023 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hibino K, A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition. Biophys. J. 97 (5): 1277-1287 (2009). PMID 19720015.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content MAP kinases (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13254_A5D8.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13254_A5D8a.seq check
          >13254_13254_A5D8_2_CMV-Forward_F09_18.ab1
              1 TGCACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
            121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
            181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
            241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
            301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
            361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
            421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
            481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
            541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
            601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
            661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
            721 CCCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
            781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGCCGGACTC
            841 AGATCTCGAG CTCAAGCTTC GAATTCATGG AGCACATACA GGGAGCTTGG AAGACGATCA
            901 GCAATGGTTT TGGATTCAAA GATGCCGTGT TTGATGGCTC CAGCTGCATC TCTCCTACAA
            961 TAGTTCAGCA GTTTGGCTAT CAGCGCCGGG CATCAGATGA TGGCAAACTC ACAGATCCCT
           1021 TCTAAGACAA GCAACACTAT CCCGTGTTTT CTGCCGAACA GCAAAGAACA GTGTCATGTG
           1081 CGAAATGTAT GAAGCCTGCA TGACTGCGTA ATGAAAGCAC TCATGGTGAG GGGGCCCTTG
          1141 CCAACCAGAG
          //
          Primer: EGFP-C
          Sequence file: RDB13254_A5D8b.seq check
          >13254_13254_A5D8_2_EGFP-C_G09_21.ab1
              1 CGGACTCTCG GCATGGACGA GCTGTACAAG TCCGGCCGGA CTCAGATCTC GAGCTCAAGC
             61 TTCGAATTCA TGGAGCACAT ACAGGGAGCT TGGAAGACGA TCAGCAATGG TTTTGGATTC
            121 AAAGATGCCG TGTTTGATGG CTCCAGCTGC ATCTCTCCTA CAATAGTTCA GCAGTTTGGC
            181 TATCAGCGCC GGGCATCAGA TGATGGCAAA CTCACAGATC CTTCTAAGAC AAGCAACACT
            241 ATCCGTGTTT TCTTGCCGAA CAAGCAAAGA ACAGTGGTCA ATGTGCGAAA TGGAATGAGC
            301 TTGCATGACT GCCTTATGAA AGCACTCAAG GTGAGGGGCC TGCAACCAGA GTGCTGTGCA
            361 GTGTTCAGAC TTCTCCACGA ACACAAAGGT AAAAAAGCAC GCTTAGATTG GAATACTGAT
            421 GCTGCGTCTT TGATTGGAGA AGAACTTCAA GTAGATTTCC TGGATCATGT TCCCCTCACA
            481 ACACACAACT TTGCTCGGAA GACGTTCCTG AAGCTTGCCT TCTGTGACAT CTGTCAGAAA
            541 TTCCTGCTCA ATGGATTTCG ATGTCAGACT TGTGGCTACA AATTTCATGA GCACTGTAGC
            601 ACCAAAGTAC CTACTATGTG TGTGGACTGG AGTAACATCA GACAACTCTT ATTGTTTCCA
            661 AATTCCACTA TTGGTGATAG TGGAGTCCCA GCACTACCTT CTTTGACTAT GCGTCGTATG
            721 CGAGAGTCTG TTTCCAGGAT GCCTGTTAGT TCTCAGCACA GATATTCTAC ACCTCACGCC
            781 TTCACCTTTA ACACCTCCAG TCCCTCATCT GAAGGTTCCC TCTCCCAGAG GCAGAGGTCG
            841 ACATCCACAC CTAATGTCCA CATGGTCAGC ACCACCCTGC CTGTGGACAG CAGGATGATT
            901 GAGGATGCAA TTCGAAGTCA CAGCGAATCG GCCTCACCTT CAGCCCTGTC CAGTAGCCCC
            961 AACAATCTGA GCCCAACAGG CTGGTCACAG CCGAAAACCC CCGTGCCAGC ACAAAGAGAG
           1021 CGGGCACAGT ATCTGGGACC CAGGAGAAAA AACAAAATTA GCCTCGTGAC AGAAGAGATT
           1081 CAAGCTATTA TGGGAAATAG AAGCAGTGAA GTGATGCTGT CAATTCGATG GTTCAGGCTC
          1141 TTTTGGACTG TTTTTATAAG GTAATGTGTG G
          //
          Primer: pAxEF_R2
          Sequence file: RDB13254_A5D8c.seq check
          >13254_13254_A5D8_2_pAxEF_R2_H09_24.ab1
              1 CGGGCAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
             61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
            121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
            181 ATCAGTTATC TAGATCCGGT GGATCCCTAG AAGACAGGCA GCCTCGGGGA CGTGGTCAGC
            241 GTGCAAGCAT TGATATCCTC AGTGTGGGCT GCCCGATGCA AGGATGGCTC GGAAGCGCTC
            301 CGGTTGATCT TCGGTAGAGA GTGTTGGAGC AGCTCAATGG AAGACAGGAT CTGGGGAAAA
            361 AGAGGCCTCT CTTCCTTTAC TTTCTTCACA CAGTCAGCTA CCAGCCTCTT CATTGCTTTG
            421 GGGCAGTTCT TATATAGCTT ACTAAGATCT GGGGAGGCAT ATCCTCGGCC CACCATGAAG
            481 ATGATCTGAT CTCGGTTGTT GATGTGAGAA TAAGGAAGCT CCCCCGTCAT CAGTTCATAC
            541 AATACGATGC CATAGGAGTA GACATCCGAC TGGAAACTGA ATGGGTTGTT ATCCTGCATT
            601 CGGATCACCT CTGGGGCCAT CCAGAGGACA GAGCCAGTAG GTTGTTCAAC CTGCTGAGAA
            661 CCACTCCAGC GTGACTTTAC TGTTGCCAAA CCAAAATCTC CAATTTTCAC TGTTAAGCCT
            721 TCATGGAGAA ATATATTGTT GGATTTCATG TCTCTATGGA TGATGTTCTT TGCATGCAAA
            781 TAGTCCATTC CCTGAGCCGT CTGCCGGGCA ATGTCAATTA GCTGGAACAT CTGAAACTTG
            841 GTCTCCTGGA CATGCAGGTG TTTGTAGAGG CTGCTGCCCT CGCACCACTG GGTCACAATT
            901 GCCAGGTTGT CCTTTGTCAT GTACCCCATG AAAAGCAGAA TGTTCACATG CCGTGTTTTG
            961 CGCAGAACAG CCACCTCATT CCTGAAGGCC TGGAATTGCT CTGGGGGTTG GGTCGACAAC
           1021 CTTTAGGATC TTTACTGCAA CATCTCCGTG CCATTTACCC TTATAAACAG TTCCAAAAGA
           1081 GCCTGACCCA TCCGAGTGAC AGCATCACTT TCACTTGGCC TCCTATTTTC CATTATTAGC
          1141 TGGATCCTCC TCTCTTGTTC CCACGAGGCC GTAATTTTTT GGATATC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hibino K, A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition. Biophys. J. 97 (5): 1277-1287 (2009). PMID 19720015.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl