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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

GL023 (#RDB13254)

Expression plasmid of GFP-tagged human c-RAF.

Clone info. Expression plasmid of GFP-tagged human c-RAF, GFP-RAF in Fig.1 of Hibino, K. J. Biol. Chem. 286 (42), 36460-36468 (2011). PMID 21862573.
Comment Expression was confirmed by the depositor with immunoblotting and live-cell imaging.
Vector backbone pEGFP-C2 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name Human c-raf1 cDNA
Depositor|Developer Sako, Yasushi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Biophys. J. 97, 1277-1287, 2009)
提供案内 (日本国内) [open/close]

MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biophys. J. 97, 1277-1287, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13254 GL023 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The GL023 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13254).

Reference section:

Hibino K., Shibata T, Yanagida T, Sako Y. , A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition. Biophys. J. 97 (5): 1277-1287 (2009). PMID 19720015. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13254_A5D8.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13254_A5D8a.seq check
>13254_13254_A5D8_2_CMV-Forward_F09_18.ab1
    1 TGCACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA
  121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  241 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  301 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  541 ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG
  601 TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
  661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA
  721 CCCAGTCCAA GCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT
  781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGCCGGACTC
  841 AGATCTCGAG CTCAAGCTTC GAATTCATGG AGCACATACA GGGAGCTTGG AAGACGATCA
  901 GCAATGGTTT TGGATTCAAA GATGCCGTGT TTGATGGCTC CAGCTGCATC TCTCCTACAA
  961 TAGTTCAGCA GTTTGGCTAT CAGCGCCGGG CATCAGATGA TGGCAAACTC ACAGATCCCT
 1021 TCTAAGACAA GCAACACTAT CCCGTGTTTT CTGCCGAACA GCAAAGAACA GTGTCATGTG
 1081 CGAAATGTAT GAAGCCTGCA TGACTGCGTA ATGAAAGCAC TCATGGTGAG GGGGCCCTTG
1141 CCAACCAGAG
//
Primer: EGFP-C
Sequence file: RDB13254_A5D8b.seq check
>13254_13254_A5D8_2_EGFP-C_G09_21.ab1
    1 CGGACTCTCG GCATGGACGA GCTGTACAAG TCCGGCCGGA CTCAGATCTC GAGCTCAAGC
   61 TTCGAATTCA TGGAGCACAT ACAGGGAGCT TGGAAGACGA TCAGCAATGG TTTTGGATTC
  121 AAAGATGCCG TGTTTGATGG CTCCAGCTGC ATCTCTCCTA CAATAGTTCA GCAGTTTGGC
  181 TATCAGCGCC GGGCATCAGA TGATGGCAAA CTCACAGATC CTTCTAAGAC AAGCAACACT
  241 ATCCGTGTTT TCTTGCCGAA CAAGCAAAGA ACAGTGGTCA ATGTGCGAAA TGGAATGAGC
  301 TTGCATGACT GCCTTATGAA AGCACTCAAG GTGAGGGGCC TGCAACCAGA GTGCTGTGCA
  361 GTGTTCAGAC TTCTCCACGA ACACAAAGGT AAAAAAGCAC GCTTAGATTG GAATACTGAT
  421 GCTGCGTCTT TGATTGGAGA AGAACTTCAA GTAGATTTCC TGGATCATGT TCCCCTCACA
  481 ACACACAACT TTGCTCGGAA GACGTTCCTG AAGCTTGCCT TCTGTGACAT CTGTCAGAAA
  541 TTCCTGCTCA ATGGATTTCG ATGTCAGACT TGTGGCTACA AATTTCATGA GCACTGTAGC
  601 ACCAAAGTAC CTACTATGTG TGTGGACTGG AGTAACATCA GACAACTCTT ATTGTTTCCA
  661 AATTCCACTA TTGGTGATAG TGGAGTCCCA GCACTACCTT CTTTGACTAT GCGTCGTATG
  721 CGAGAGTCTG TTTCCAGGAT GCCTGTTAGT TCTCAGCACA GATATTCTAC ACCTCACGCC
  781 TTCACCTTTA ACACCTCCAG TCCCTCATCT GAAGGTTCCC TCTCCCAGAG GCAGAGGTCG
  841 ACATCCACAC CTAATGTCCA CATGGTCAGC ACCACCCTGC CTGTGGACAG CAGGATGATT
  901 GAGGATGCAA TTCGAAGTCA CAGCGAATCG GCCTCACCTT CAGCCCTGTC CAGTAGCCCC
  961 AACAATCTGA GCCCAACAGG CTGGTCACAG CCGAAAACCC CCGTGCCAGC ACAAAGAGAG
 1021 CGGGCACAGT ATCTGGGACC CAGGAGAAAA AACAAAATTA GCCTCGTGAC AGAAGAGATT
 1081 CAAGCTATTA TGGGAAATAG AAGCAGTGAA GTGATGCTGT CAATTCGATG GTTCAGGCTC
1141 TTTTGGACTG TTTTTATAAG GTAATGTGTG G
//
Primer: pAxEF_R2
Sequence file: RDB13254_A5D8c.seq check
>13254_13254_A5D8_2_pAxEF_R2_H09_24.ab1
    1 CGGGCAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG
  121 AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG
  181 ATCAGTTATC TAGATCCGGT GGATCCCTAG AAGACAGGCA GCCTCGGGGA CGTGGTCAGC
  241 GTGCAAGCAT TGATATCCTC AGTGTGGGCT GCCCGATGCA AGGATGGCTC GGAAGCGCTC
  301 CGGTTGATCT TCGGTAGAGA GTGTTGGAGC AGCTCAATGG AAGACAGGAT CTGGGGAAAA
  361 AGAGGCCTCT CTTCCTTTAC TTTCTTCACA CAGTCAGCTA CCAGCCTCTT CATTGCTTTG
  421 GGGCAGTTCT TATATAGCTT ACTAAGATCT GGGGAGGCAT ATCCTCGGCC CACCATGAAG
  481 ATGATCTGAT CTCGGTTGTT GATGTGAGAA TAAGGAAGCT CCCCCGTCAT CAGTTCATAC
  541 AATACGATGC CATAGGAGTA GACATCCGAC TGGAAACTGA ATGGGTTGTT ATCCTGCATT
  601 CGGATCACCT CTGGGGCCAT CCAGAGGACA GAGCCAGTAG GTTGTTCAAC CTGCTGAGAA
  661 CCACTCCAGC GTGACTTTAC TGTTGCCAAA CCAAAATCTC CAATTTTCAC TGTTAAGCCT
  721 TCATGGAGAA ATATATTGTT GGATTTCATG TCTCTATGGA TGATGTTCTT TGCATGCAAA
  781 TAGTCCATTC CCTGAGCCGT CTGCCGGGCA ATGTCAATTA GCTGGAACAT CTGAAACTTG
  841 GTCTCCTGGA CATGCAGGTG TTTGTAGAGG CTGCTGCCCT CGCACCACTG GGTCACAATT
  901 GCCAGGTTGT CCTTTGTCAT GTACCCCATG AAAAGCAGAA TGTTCACATG CCGTGTTTTG
  961 CGCAGAACAG CCACCTCATT CCTGAAGGCC TGGAATTGCT CTGGGGGTTG GGTCGACAAC
 1021 CTTTAGGATC TTTACTGCAA CATCTCCGTG CCATTTACCC TTATAAACAG TTCCAAAAGA
 1081 GCCTGACCCA TCCGAGTGAC AGCATCACTT TCACTTGGCC TCCTATTTTC CATTATTAGC
1141 TGGATCCTCC TCTCTTGTTC CCACGAGGCC GTAATTTTTT GGATATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hibino K, A RasGTP-induced conformational change in C-RAF is essential for accurate molecular recognition. Biophys. J. 97 (5): 1277-1287 (2009). PMID 19720015. [link to RRC of NBRP]