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pSpCas9(BB)-2A-puro-ACAT1 sgRNA (#RDB14697)

Expression vector of sgRNA for targeting human ACAT1 and Cas9.

Clone info. Expression vector of sgRNA for targeting human acyl-coenzyme A: cholesterol acyltransferase type 1 (ACAT1), SpCAS9 and 2A-Puro.
Comment Expression was confirmed by the depositor with fluorescence microscopy. Recommended host E. coli strain, stbl3.
Vector backbone PX459 (plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human ACAT1 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14697 pSpCas9(BB)-2A-puro-ACAT1 sgRNA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pSpCas9(BB)-2A-puro-ACAT1 sgRNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14697).

Reference section:

Makino, A., Hullin-Matsuda, F., Murate, M., Abe, M., Tomishige, N., Fukuda, M., Yamashita, S., Fujimoto, T., Vidal, H., Lagarde, M., Delton, I., Kobayashi, T., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]