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pDEST/mCherry-Rab18

Expression vector of human Rab18, wild type, fused with mCherry.

Catalog number RDB14699
Resource name pDEST/mCherry-Rab18
Clone info. Expression vector of human Rab18, wild type, fused with mCherry.
Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |
 
Other clones in our bank human RAB18 (NCBI Gene 22931) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
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Please contact us. UCSD's MTA is also required.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB14699 pDEST/mCherry-Rab18 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14699_A7Djp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F RDB14699_A7Dja.seq
>D04483A3_A7Dj_1_mCherry-C_F_A05_02_ABI24.ab1
    1 TAACAAGCAG CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC
   61 TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC
  121 ACCGGCGGCA TGGACGAGCT GTACAAGAAG CTTACAAGTT TGTACAAAAA AGCAGGCTCC
  181 GCGGCCGCCT TGTTTAACTT TAAGAAGGAG CCCTTCACCA TGGACGAGGA CGTGCTAACC
  241 ACCCTGAAGA TCCTCATCAT CGGCGAGAGT GGGGTGGGCA AGTCCAGCCT GCTCTTGAGG
  301 TTCACAGATG ATACGTTTGA TCCAGAACTT GCAGCAACAA TAGGTGTTGA CTTTAAGGTG
  361 AAAACAATTT CAGTGGATGG AAATAAGGCT AAACTTGCAA TATGGGATAC TGCTGGTCAA
  421 GAGAGGTTTA GAACATTAAC TCCCAGCTAT TATAGAGGTG CACAGGGTGT TATATTAGTT
  481 TATGATGTCA CAAGAAGAGA TACATTTGTT AAACTGGATA ATTGGTTAAA TGAATTGGAA
  541 ACATACTGTA CAAGAAATGA CATAGTAAAC ATGCTAGTTG GAAATAAAAT CGATAAGGAA
  601 AATCGTGAAG TCGATAGAAA TGAAGGCCTG AAATTTGCAC GAAAGCATTC CATGTTATTT
  661 ATAGAGGCAA GTGCAAAAAC CTGTGATGGT GTACAATGTG CCTTTGAAGA ACTTGTTGAA
  721 AAGATCATTC AGACCCCTGG ACTGTGGGAA AGTGAGAACC AGAATAAAGG AGTCAAACTG
  781 TCACACAGGG AAGAAGGCCA AGGAGGAGGA GCCTGTGGTG GTTATTGCTC TGTGTTATAA
  841 AAGGGTGGGC GCGCCGACCC AGCTTTCTTG TACAAAGTGG TTGATCTAGA GGGCCCGCGG
  901 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
  961 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
 1021 CAGCATCTGT GTTGCCCCTC CCCCGTGCCT TCATGACCTG AGTGCCACTC CCACTGTCGT
 1081 TCTTATTAAT GAAGAAATGC ATCGCATTGT TGATAGTTCA TTCTATCTGG GGGGTTGGGA
1141 TTGAGCCAGG AA
//
Primer: BGH_rev2 RDB14699_A7Djb.seq
>D04483A3_A7Dj_1_BGH_rev2_B05_05_ABI24.ab1
    1 GTATTCCATA AGCAAGTAAC ACGTCACACA GGGCAGCAGC AGATGGCGTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACTCAATGGT GATGGTGATG ATGACCGGTA
  121 CGCGTAGAAT CGAGACCGAG GAGAGGGTTA GGGATAGGCT TACCTTCGAA CCGCGGGCCC
  181 TCTAGATCAA CCACTTTGTA CAAGAAAGCT GGGTCGGCGC GCCCACCCTT TTATAACACA
  241 GAGCAATAAC CACCACAGGC TCCTCCTCCT TGGCCTTCTT CCCTGTGTGA CAGTTTGACT
  301 CCTTTATTCT GGTTCTCACT TTCCCACAGT CCAGGGGTCT GAATGATCTT TTCAACAAGT
  361 TCTTCAAAGG CACATTGTAC ACCATCACAG GTTTTTGCAC TTGCCTCTAT AAATAACATG
  421 GAATGCTTTC GTGCAAATTT CAGGCCTTCA TTTCTATCGA CTTCACGATT TTCCTTATCG
  481 ATTTTATTTC CAACTAGCAT GTTTACTATG TCATTTCTTG TACAGTATGT TTCCAATTCA
  541 TTTAACCAAT TATCCAGTTT AACAAATGTA TCTCTTCTTG TGACATCATA AACTAATATA
  601 ACACCCTGTG CACCTCTATA ATAGCTGGGA GTTAATGTTC TAAACCTCTC TTGACCAGCA
  661 GTATCCCATA TTGCAAGTTT AGCCTTATTT CCATCCACTG AAATTGTTTT CACCTTAAAG
  721 TCAACACCTA TTGTTGCTGC AAGTTCTGGA TCAAACGTAT CATCTGTGAA CCTCAAGAGC
  781 AGGCTGGACT TGCCCACCCC ACTCTCGCCG ATGATGAGGA TCTTCAGGGT GGTTAGCACG
  841 TCCTCGTCCA TGGTGAAGGG CTCCTTCTTA AAGTTAAACA AGGCGGCCGC GGAGCCTGCT
  901 TTTTTGTACA AACTTGTAAG CTTCTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG
  961 CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC
 1021 CACTTGATGT TGACGTTGTA GCGCCGGCAG CTGCACGGGC TCTGGCTTGT AGTGTCTGGA
 1081 CTCAGCGTCG TATGATGCGG TCTTCAGCTT CAGCTCTGCT TGGATCTCGC CCATCAGGAC
1141 GCCGATCCCT CGGGGGTAC
//
Primer: CMV-Forward RDB14699_A7Djc.seq
>D04483A3_A7Dj_1_CMV-Forward_C05_08_ABI24.ab1
    1 ACTAATCAAC TTTCCGCCCC AATTGACGCA AATGGGCGGT AGGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTATCAG GTACCATGGT
  181 GAGCAAGGGC GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA
  241 CATGGAGGGC TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC
  301 CTACGAGGGC ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC
  361 CTGGGACATC CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC
  421 CGACATCCCC GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT
  481 GAACTTCGAG GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA
  541 GTTCATCTAC AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA
  601 GAAGAAGACC ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT
  661 GAAGGGCGAG ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT
  721 CAAGACCACC TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT
  781 CAAGTTGGAC ATCACCTCCC ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC
  841 CGAGGGCCGC CACTCCACCG GCGGCATGGA CGAGCTGTAC AAGAAGCTTA CAAGTTTGTA
  901 CAAAAAAGCA GGCTCCGCGG CCGCCTTGTT TAACTTTAAG AAGGAGCCCT TCACCATGGA
  961 CGAGGACGTG CTAACCACCC TGAAGATCCT CATCATCGGC GAGAGTGGGG TGGGGCAAGT
 1021 CCAGCCCTGC TCTTGAGGTC ACAGATGATA CGGTTTGATC CAGACTGCAG CACAATAGGT
 1081 GTGACTTAGG TGAAACAATT TTCATGATGA ATTAGGCCTT AACTGCAATA TGGGATAACC
1141 TGCCTGGGTC AAGAAGAGGA GAAGT
//
Primer: SV40pro_F RDB14699_A7Djd.seq
>D04483A3_A7Dj_1_SV40pro_F_A01_01_ABI24.ab1
    1 CCCCGTCCTT ACTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCCGC CCTTCTATGA AAGGTTGGGC
 1021 TTTCGGGAAT CGTTTTCCGG GGACGCCCGG GCTGGGATGA TCCTCCAGCG CGGGGATCTC
 1081 ATGCTGCAGT CTTCGCCCAC CCCCAAACTG GTTATGGCCA GCCTATATTG TTACAATTAA
1141 GCAGTAGCCA TCAACAAATT TTCGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl