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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-GFP-ERK2

Expression vector of GFP-tagged human ERK2.

Catalog number RDB14156
Resource name pCMV-GFP-ERK2
Alternative name GL 113
Clone info. Expression vector of GFP-tagged human ERK2.
Comment Expression was confirmed by the depositor.
Vector backbone pEGFP-C2 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name human ERK2 cDNA
Depositor|Developer Sako, Yasushi |
Other clones in our bank

External Database
human ERK2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14156 pCMV-GFP-ERK2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14156_A6Ecp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14156_A6Eca.seq check
          >D03441A3_A6Ec_2_CMV_Forward_C05_08.ab1
              1 ACATCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
            121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
            181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
            241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
            301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
            361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
            421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
            481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
            541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
            601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
            661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
            721 CACCCAGTCC AAAGCTGAGC AAAGGACCCC AACGAGAAGC GCGATCACAT GGGTCCTGCT
            781 GGAGTTCGTG ACCGCCGCCG GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGGC
            841 CGGACTCAGA TCCAAGGCTT CGAATTCTGC AGTCGACGGT ACCCGCCGGG CCCATCAACA
            901 AGTTTGTACA AAAAAGCAAG GGCTTTATCC CGGGCAGCCC CATATGGCGG CGGCGGGCCG
            961 GCCGGGCGGG GCGCGGCCTC AGGAGAATGG TTCCCGCTGA GGTCAGTGTT CCGACGGTGA
           1021 GAGCCGGCCG CCTACACCCA AAAACCCTCT TGTTACCATC CGGCCGAAGG GCAGTCAATA
          1081 ACGGAGTTAC AAT
          //
          Primer: EGFP-C
          Sequence file: RDB14156_A6Ecb.seq check
          >D03441A3_A6Ec_2_EGFP_C_D05_11.ab1
              1 CGAAGGTTAT CTCGGGCATG GAGAGCTGTA CAGTCCGGCC GGACTCAGAT CCAAGCTTCG
             61 AATTCTGCAG TCGACGGTAC CGCGGGCCCA TCAACAAGTT TGTACAAAAA AGCAGGCTTT
            121 ATCCCGGCAG CCCATATGGC GGCGGCGGCG GCGGCGGGCG CGGGCCCGGA GATGGTCCGC
            181 GGGCAGGTGT TCGACGTGGG GCCGCGCTAC ACCAACCTCT CGTACATCGG CGAGGGCGCC
            241 TACGGCATGG TGTGCTCTGC TTATGATAAT GTCAACAAAG TTCGAGTAGC TATCAAGAAA
            301 ATCAGCCCCT TTGAGCACCA GACCTACTGC CAGAGAACCC TGAGGGAGAT AAAAATCTTA
            361 CTGCGCTTCA GACATGAGAA CATCATTGGA ATCAATGACA TTATTCGAGC ACCAACCATC
            421 GAGCAAATGA AAGATGTATA TATAGTACAG GACCTCATGG AAACAGATCT TTACAAGCTC
            481 TTGAAGACAC AACACCTCAG CAATGACCAT ATCTGCTATT TTCTCTACCA GATCCTCAGA
            541 GGGTTAAAAT ATATCCATTC AGCTAACGTT CTGCACCGTG ACCTCAAGCC TTCCAACCTG
            601 CTGCTCAACA CCACCTGTGA TCTCAAGATC TGTGACTTTG GCCTGGCCCG TGTTGCAGAT
            661 CCAGACCATG ATCACACAGG GTTCCTGACA GAATATGTGG CCACACGTTG GTACAGGGCT
            721 CCAGAAATTA TGTTGAATTC CAAGGGCTAC ACCAAGTCCA TTGATATTTG GTCTGTAGGC
            781 TGCATTCTGG CAGAAATGCT TTCTAACAGG CCCATCTTTC CAGGGAAAGC ATTATCTTGA
            841 CCAGCTGAAC CACATTTTGG GGTATTCTTG GGATCCCCCA TCACAAGAAA GACCTGAATT
            901 GTATAAATAA ATTTTAAAAA GCTAGGAAAC TATTTTGCTT TTCTCTTTCC ACACAAAAAT
            961 AAAGGTGCCC ATGGGAACAG GCTGTTCCCA AATGGCTGGA CTCCCAAGCT TCTGGACTTT
           1021 ATTGGACAAA AAATGGTGGA CATTTCCAAT CCCACACTAG GAGGATGAGT TAGCAAACAG
          1081 GCCTCTGGGC CCAAC
          //
          Primer: ME_1290Rv
          Sequence file: RDB14156_A6Ecc.seq check
          >D03441A3_A6Ec_2_ME_1290RV_E05_14.ab1
              1 ATGGCCAATT CATTTTATGG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
             61 AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT
            121 CCCATCAACC ACTTTGTACA AGAAAGCTGG GTCTAGATAT CTCGAGAACT AGTGATCCTC
            181 TGAGCCCTTG TCCTGACAAA TTTAAGATCT GTATCCTGGC TGGAATCTAG CAGTCTCTTC
            241 AAAAATTAGT TCTTTGAGCT TTTCCTTAGG CAAGTCATCC AATTCCATGT CGAACTTGAA
            301 TGGTGCTTCG GCGATGGGCT CGTCACTCGG GTCGTAATAC TGCTCCAGAT ATGGGTGGGC
            361 CAGAGCCTGT TCTACTTCAA TCCTCTTGTG TGGGTTGAAT GTCAACATTT TGTCCAATAA
            421 GTCCAGAGCT TTGGAGTCAG CATTTGGGAA CAGCCTGTTC CATGGCACCT TATTTTTGTG
            481 TGGAAGAGAA AGCAAATAGT TCCTAGCTTT TAAATTTATT ATACAATTCA GGTCTTCTTG
            541 TGATGGGGAT CCAAGAATAC CCAAAATGTG GTTCAGCTGG TCAAGATAAT GCTTCCCTGG
            601 AAAGATGGGC CTGTTAGAAA GCATTTCTGC CAGAATGCAG CCTACAGACC AAATATCAAT
            661 GGACTTGGTG TAGCCCTTGG AATTCAACAT AATTTCTGGA GCCCTGTACC AACGTGTGGC
            721 CACATATTCT GTCAGGAACC CTGTGTGATC ATGGTCTGGA TCTGCAACAC GGGCCAGGCC
            781 AAAGTCACAG ATCTTGAGAT CACAGGTGGT GTTGAGCAGC AGGTTGGAAG GCTTGAGGGT
            841 CACGGTGCAG AACGTTAGCT GAATGGATAT ATTTTTAACC CCTCTGAGGA TCTGGTAGAG
            901 AAAATAGCAG ATATGGGTCA TTGGCTGAAG GTGGTTGTGT CTTCAAGAGC TTTGTAAAGA
            961 TCTGTTTCCA TGAGGTCCCT GTACTATATA TACATCTTTC ATTGCTCGAT GGTTTGGGTG
           1021 CTCGGAATAA TGTCATTGGA TTTCCATGGA ATGGTTCTCA AGTATCTGAG GCGCCAGTTA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14156_A6Ecd.seq check
          >D03441A3_A6Ec_2_SV40pro_F_V2_F05_17.ab1
              1 GTGGGCGTTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
             61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
            601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
            721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
            841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
            901 CCCCAACCTG CCATCACGAG ATTTCGATTC CACCGGCCGC CCTTCTATGA AAGTTTGGGG
            961 CTTCGGAATC GTTTTTCCCG GGGACGGCCG GCTTGGAATG GATCCCTTCC AAGCGGCGGG
          1021 GAATCCTCAT GGCTTGAAGA TCATTCGCCT ATCCCTTAGG GGGGATGGCC TAACC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl