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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-GFP-ERK2 (#RDB14156)

Expression vector of GFP-tagged human ERK2.

Alternative name GL 113
Clone info. Expression vector of GFP-tagged human ERK2.
Comment Expression was confirmed by the depositor.
Vector backbone pEGFP-C2 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r
Gene/insert name human ERK2 cDNA
Depositor|Developer Sako, Yasushi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14156 pCMV-GFP-ERK2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-GFP-ERK2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14156).

Reference section:

Shindo, Y., Iwamoto, K., Mouri, K., Hibino, K., Tomita, M., Kosako, H., Sako, Y., Takahashi, K., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14156_A6Ecp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14156_A6Eca.seq check
>D03441A3_A6Ec_2_CMV_Forward_C05_08.ab1
    1 ACATCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
  721 CACCCAGTCC AAAGCTGAGC AAAGGACCCC AACGAGAAGC GCGATCACAT GGGTCCTGCT
  781 GGAGTTCGTG ACCGCCGCCG GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGGC
  841 CGGACTCAGA TCCAAGGCTT CGAATTCTGC AGTCGACGGT ACCCGCCGGG CCCATCAACA
  901 AGTTTGTACA AAAAAGCAAG GGCTTTATCC CGGGCAGCCC CATATGGCGG CGGCGGGCCG
  961 GCCGGGCGGG GCGCGGCCTC AGGAGAATGG TTCCCGCTGA GGTCAGTGTT CCGACGGTGA
 1021 GAGCCGGCCG CCTACACCCA AAAACCCTCT TGTTACCATC CGGCCGAAGG GCAGTCAATA
1081 ACGGAGTTAC AAT
//
Primer: EGFP-C
Sequence file: RDB14156_A6Ecb.seq check
>D03441A3_A6Ec_2_EGFP_C_D05_11.ab1
    1 CGAAGGTTAT CTCGGGCATG GAGAGCTGTA CAGTCCGGCC GGACTCAGAT CCAAGCTTCG
   61 AATTCTGCAG TCGACGGTAC CGCGGGCCCA TCAACAAGTT TGTACAAAAA AGCAGGCTTT
  121 ATCCCGGCAG CCCATATGGC GGCGGCGGCG GCGGCGGGCG CGGGCCCGGA GATGGTCCGC
  181 GGGCAGGTGT TCGACGTGGG GCCGCGCTAC ACCAACCTCT CGTACATCGG CGAGGGCGCC
  241 TACGGCATGG TGTGCTCTGC TTATGATAAT GTCAACAAAG TTCGAGTAGC TATCAAGAAA
  301 ATCAGCCCCT TTGAGCACCA GACCTACTGC CAGAGAACCC TGAGGGAGAT AAAAATCTTA
  361 CTGCGCTTCA GACATGAGAA CATCATTGGA ATCAATGACA TTATTCGAGC ACCAACCATC
  421 GAGCAAATGA AAGATGTATA TATAGTACAG GACCTCATGG AAACAGATCT TTACAAGCTC
  481 TTGAAGACAC AACACCTCAG CAATGACCAT ATCTGCTATT TTCTCTACCA GATCCTCAGA
  541 GGGTTAAAAT ATATCCATTC AGCTAACGTT CTGCACCGTG ACCTCAAGCC TTCCAACCTG
  601 CTGCTCAACA CCACCTGTGA TCTCAAGATC TGTGACTTTG GCCTGGCCCG TGTTGCAGAT
  661 CCAGACCATG ATCACACAGG GTTCCTGACA GAATATGTGG CCACACGTTG GTACAGGGCT
  721 CCAGAAATTA TGTTGAATTC CAAGGGCTAC ACCAAGTCCA TTGATATTTG GTCTGTAGGC
  781 TGCATTCTGG CAGAAATGCT TTCTAACAGG CCCATCTTTC CAGGGAAAGC ATTATCTTGA
  841 CCAGCTGAAC CACATTTTGG GGTATTCTTG GGATCCCCCA TCACAAGAAA GACCTGAATT
  901 GTATAAATAA ATTTTAAAAA GCTAGGAAAC TATTTTGCTT TTCTCTTTCC ACACAAAAAT
  961 AAAGGTGCCC ATGGGAACAG GCTGTTCCCA AATGGCTGGA CTCCCAAGCT TCTGGACTTT
 1021 ATTGGACAAA AAATGGTGGA CATTTCCAAT CCCACACTAG GAGGATGAGT TAGCAAACAG
1081 GCCTCTGGGC CCAAC
//
Primer: ME_1290Rv
Sequence file: RDB14156_A6Ecc.seq check
>D03441A3_A6Ec_2_ME_1290RV_E05_14.ab1
    1 ATGGCCAATT CATTTTATGG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
   61 AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT
  121 CCCATCAACC ACTTTGTACA AGAAAGCTGG GTCTAGATAT CTCGAGAACT AGTGATCCTC
  181 TGAGCCCTTG TCCTGACAAA TTTAAGATCT GTATCCTGGC TGGAATCTAG CAGTCTCTTC
  241 AAAAATTAGT TCTTTGAGCT TTTCCTTAGG CAAGTCATCC AATTCCATGT CGAACTTGAA
  301 TGGTGCTTCG GCGATGGGCT CGTCACTCGG GTCGTAATAC TGCTCCAGAT ATGGGTGGGC
  361 CAGAGCCTGT TCTACTTCAA TCCTCTTGTG TGGGTTGAAT GTCAACATTT TGTCCAATAA
  421 GTCCAGAGCT TTGGAGTCAG CATTTGGGAA CAGCCTGTTC CATGGCACCT TATTTTTGTG
  481 TGGAAGAGAA AGCAAATAGT TCCTAGCTTT TAAATTTATT ATACAATTCA GGTCTTCTTG
  541 TGATGGGGAT CCAAGAATAC CCAAAATGTG GTTCAGCTGG TCAAGATAAT GCTTCCCTGG
  601 AAAGATGGGC CTGTTAGAAA GCATTTCTGC CAGAATGCAG CCTACAGACC AAATATCAAT
  661 GGACTTGGTG TAGCCCTTGG AATTCAACAT AATTTCTGGA GCCCTGTACC AACGTGTGGC
  721 CACATATTCT GTCAGGAACC CTGTGTGATC ATGGTCTGGA TCTGCAACAC GGGCCAGGCC
  781 AAAGTCACAG ATCTTGAGAT CACAGGTGGT GTTGAGCAGC AGGTTGGAAG GCTTGAGGGT
  841 CACGGTGCAG AACGTTAGCT GAATGGATAT ATTTTTAACC CCTCTGAGGA TCTGGTAGAG
  901 AAAATAGCAG ATATGGGTCA TTGGCTGAAG GTGGTTGTGT CTTCAAGAGC TTTGTAAAGA
  961 TCTGTTTCCA TGAGGTCCCT GTACTATATA TACATCTTTC ATTGCTCGAT GGTTTGGGTG
 1021 CTCGGAATAA TGTCATTGGA TTTCCATGGA ATGGTTCTCA AGTATCTGAG GCGCCAGTTA
//
Primer: SV40pro_F_V2
Sequence file: RDB14156_A6Ecd.seq check
>D03441A3_A6Ec_2_SV40pro_F_V2_F05_17.ab1
    1 GTGGGCGTTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG AAATGACCGA CCAAGCGACG
  901 CCCCAACCTG CCATCACGAG ATTTCGATTC CACCGGCCGC CCTTCTATGA AAGTTTGGGG
  961 CTTCGGAATC GTTTTTCCCG GGGACGGCCG GCTTGGAATG GATCCCTTCC AAGCGGCGGG
1021 GAATCCTCAT GGCTTGAAGA TCATTCGCCT ATCCCTTAGG GGGGATGGCC TAACC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP]