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pDEST/mCherry-TubbyC

Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C).

Catalog number RDB16766
Resource name pDEST/mCherry-TubbyC
Clone info. Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C) fused with mCherry at N-terminus.
Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TUB cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |
 
Other clones in our bank human TUB (NCBI Gene 7275) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012) is requested.
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Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB16766 pDEST/mCherry-TubbyC DNA solution

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References and tips

Electronic file

Featured content

References

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16766_A8Kmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Red-C_F RDB16766_A8Kma.seq
>D05982A3_A8Km_1_Red-C_F_C09_09_ABI24.ab1
    1 CAACAAATCA GTTGGACTCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA
   61 ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGG GTGGAGGTAC
  121 CACTAGTGAT GAGGAGGAGA ATAGCTCCAG CTCCTCCCAG CTAAATAGTA ACACCCGCCC
  181 CAGCTCTGCT ACTAGCAGGA AGTCCGTCAG GGAGGCAGCC TCAGCCCCTA GCCCAACAGC
  241 TCCAGAGCAA CCAGTGGACG TTGAGGTCCA GGATCTTGAG GAGTTTGCAC TGAGGCCGGC
  301 CCCCCAGGGT ATCACCATCA AATGCCGCAT CACTCGGGAC AAGAAAGGGA TGGACCGGGG
  361 CATGTACCCC ACCTACTTTC TGCACCTGGA CCGTGAGGAT GGGAAGAAGG TGTTCCTCCT
  421 GGCGGGAAGG AAGAGAAAGA AGAGTAAAAC TTCCAATTAC CTCATCTCTG TGGACCCAAC
  481 AGACTTGTCT CGAGGAGGGG ACAGCTATAT CGGGAAACTG CGGTCCAACT TGATGGGCAC
  541 CAAGTTCACT GTTTATGACA ATGGAGTCAA CCCTCAGAAG GCCTCATCCT CCACTTTGGA
  601 AAGTGGAACC TTACGTCAGG AGCTGGCAGC TGTGTGCTAC GAGACAAACG TCTTAGGCTT
  661 CAAGGGGCCT CGGAAGATGA GCGTGATTGT CCCAGGCATG AACATGGTTC ATGAGAGAGT
  721 CTCTATCCGC CCCCGCAACG AGCATGAGAC ACTGCTAGCA CGCTGGCAGA ATAAGAACAC
  781 GGAGAGTATC ATCGAGCTGC AAAACAAGAC ACCTGTCTGG AATGATGACA CACAGTCCTA
  841 TGTACTCAAC TTCCATGGGC GCGTCACACA GGCCTCCGTG AAGAACTTCC AGATCATCCA
  901 TGGCAATGAC CCGGACTACA TCGTGATGCA GTTTGGCCGG GTAGCAGAGG ATGTGTTCAC
  961 CATGGATTAC AACTACCCGC TGTGTGCACT GCAGGCCTTT GCCCATTGCC CTGTCCAGCT
 1021 TCGACAGCAA GCTGGGCGTG CGAGTAGAGA TCCGTTTAAA CCCCGCTGAA TCAGCCTCGA
 1081 CCTGTGCCAT TCTATTGCCA GGCCATCTGC TGACTTTGGC CCCGTCCCCC CGATGCCATT
1141 CCATTTGGAA GCATG
//
Primer: F1ori_F RDB16766_A8Kmb.seq
>D05982A3_A8Km_1_f1ori_F_C11_03_ABI08.ab1
    1 GCGGGGCCCC TGGGGGCGCT GGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC
   61 CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTGGGGATAC CCCCTAGAGC CCCAGCTGGT
  121 TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC
  181 CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT
  241 CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC
  301 CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
  361 AGTCGAGGCT GATCAGCGGG TTTAAACGGA TCTCTACTCG CACGCCAGCT TGCTGTCGAA
  421 GCTGGACAGG GCAATGGCAA AGGCCTGCAG TGCACACAGC GGGTAGTTGT AATCCATGGT
  481 GAACACATCC TCTGCTACCC GGCCAAACTG CATCACGATG TAGTCCGGGT CATTGCCATG
  541 GATGATCTGG AAGTTCTTCA CGGAGGCCTG TGTGACGCGC CCATGGAAGT TGAGTACATA
  601 GGACTGTGTG TCATCATTCC AGACAGGTGT CTTGTTTTGC AGCTCGATGA TACTCTCCGT
  661 GTTCTTATTC TGCCAGCGTG CTAGCAGTGT CTCATGCTCG TTGCGGGGGC GGATAGAGAC
  721 TCTCTCATGA ACCATGTTCA TGCCTGGGAC AATCACGCTC ATCTTCCGAG GCCCCTTGAA
  781 GCCTAAGACG TTTGTCTCGT AGCACACAGC TGCCAGCTCC TGACGTAAGG TTCCACTTTC
  841 CAAGTGGAGG ATGAGGCCTT CTGAGGGTTG ACTCCATTGT CATAAACAGT GAACTTGGTG
  901 CCCATCAAGT TGGACCGCAG TTTCCCGATA TAGCTGTCCC CTCCTCGAGA CAAGTCTGTT
  961 GGGTCCACAG AGATGAGGTA ATTTGGAAGT TTTACTCTTC TTTCTCTTCC TTCCCGCCAG
 1021 GGAGGAACAC CTTTCTTCCC ATCCTCACGG TCCAAGGTGC AGAAAGTAGT GGGGTACATG
 1081 CCCCCGGGTC CATCCCTTTT CTTGTCCCGG AGTGATGCGG CATTGATGGT TGATACCTGT
1141 GGGACCGACC TCAGATGCAA ACCTCCCTTC AAAGAATTCC T
//
Primer: CMV_Forward RDB16766_A8Kmc.seq
>D05982A3_A8Km_1_CMV_Forward_A09_03_ABI24.ab1
    1 TGGATCCACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA
  181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
  361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
  481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
  541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
  601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
  661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
  721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
  781 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
  841 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGT GGAGGTACCA CTAGTGATGA
  901 GGAGGAGAAT AGCTCCAGCT CCTCCCAGCT AAATAGTAAC ACCCGCCCCA GCTCTGCTAC
  961 TAGCAGGAAG TCCGTCAGGA GGCAGCCCTC AGCCCCTAGC CCAACAGCTC CAGAGCAACC
 1021 AGTGGACGTT TGAGGGTCCA GGATCTTGAG AGTTTTGCAC TGAGCCCGGG CCCCCCCACG
 1081 TATCACCATC AATGCCGCAT CACTCGGACA AGAAGGTATG GGAACCGGGG CAATCTACCC
1141 CCCCACCTTA CCTATC
//
Primer: SV40pro_F RDB16766_A8Kmd.seq
>D05982A3_A8Km_1_SV40pro_F_D09_12_ABI24.ab1
    1 CCCTTGGTCC TTGGCTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG
  961 ACGCCCAACC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCCTTCTAT GAAAGGTTGG
 1021 GCTTCGGAAT CGTTTTCCGG GACGCCGGGC TGGGATGATC CTCCAGCGCG GGGATCTCAT
 1081 GCTGCAGGTT CTTTCGCCCA CCCCGACTGG TTAATGGCAG CTTATATGTT TAACAAATAA
1141 CGGCCAATAA GCAATTCACA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl