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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-TubbyC (#RDB16766)

Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C).

Clone info. Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C) fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TUB cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012）の引用を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16766 pDEST/mCherry-TubbyC DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-TubbyC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16766).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16766_A8Kmp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'(mCherry 5')
Sequence file: RDB16766_A8Kme.seq check
>D05982A3_A8Km_1_CMV_Forward_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT GNNGCNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA
  181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
  361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
  421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
  481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
  541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
  601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
  661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG ANGTCAAGAC
  721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
  781 GGACATCACC TCCCACAACG AGGNNTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
  841 CCGCCACTCC ACCGGCGGCA TGGANGANCT GTACAAGGGT GGANGTACCA CTAGNGATGA
  901 NGANGANAAT AGCTNNNGCT NCTNCCANCN AAATAGTAAC ACCCGCCCCA GCNNNGCTAC
  961 TANCNNNANN NNCGNCNNNN NNNAGNCNNN AGCCCNNAGC CNNNCNGCTN NNNANCNNNN
1021 NNNNNN
//
Primer: f1ori_F (Pr0280)
Region: f1 ori,bGH pA,insert 3'(TubbyC 3')
Sequence file: RDB16766_A8Kmf.seq check
>D05982A3_A8Km_1_f1ori_F_C11_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCGGTCNCG CTGCGCGTAA CCACCACACC
   61 CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTGGGGATAC CCCCTAGAGC CCCAGCTGGT
  121 TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC
  181 CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT
  241 CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC
  301 CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
  361 AGTCGAGGCT GATCAGCGGG TTTAAACGGA TCTCTACTCG CACGCCAGCT TGCTGTCGAA
  421 GCTGGACAGG GCAATGGCAA AGGCCTGCAG TGCACACAGC GGGTAGTTGT AATCCATGGT
  481 GAACACATCC TCTGCTACCC GGCCAAACTG CATCACGATG TAGTCCGGGT CATTGCCATG
  541 GATGATCTGG AAGTTCTTCA CGGAGGCCTG TGTGACGCGC CCATGGAAGT TGAGTACATA
  601 GGACTGTGTG TCATCATTCC AGACAGGTGT CTTGTTTTGC AGCTCGATGA TACTCTCCGT
  661 GTTCTTATTC TGCCAGCGTG CTAGCAGTGT CTCATGCTCG TTGCGGGGGC GGATAGAGAC
  721 TCTCTCATGA ACCATGTTCA TGCCTGGGAC AATCACGCTC ATCTTCCGAG GCCCCTTGAA
  781 GCCTAAGACG TTTGTCTCGT ANCACACAGC TGCCAGCTCC TGACGTNAGG NTCCACTTTC
  841 CAAGTGGANG ANGANGNCTT CTGAGGGTTG ANNNNNTTGN CNNNNACNGT GAACTTGGTG
  901 NCCATCANGT TGGNNCGCAG TTNNCCNANA NNNCTGTCCC CNCCTCNAGN CAANTCTGTT
  961 GGNNCCACAN ANATGANGTN ANTNGNAANT TTTANNCTNN TTTCTCTTNN TTCCNNNNNN
1021 GNANNANNNN NNTNNNNNCN NNNNNNNNN
//
Primer: mRFP1-inner1 (Pr0443)
Region: insert mid(mCherry 3',TubbyC 5')
Sequence file: RDB16766_A8Kmg.seq check
>16766_16766_B3CdF_mRFP1-inner1_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN TCCTCCCTGC AGGANGGCGA GTTCATCTAC AAGGTGAAGC
   61 TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC ATGGGCTGGG
  121 AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG ATCAAGCAGA
  181 GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGAGGT CAAGACCACC TACAAGGCCA
  241 AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC ATCACCTCCC
  301 ACAACGAGGA CTACACCATC GTGGAACAGT ACGAACGCGC CGAGGGCCGC CACTCCACCG
  361 GCGGCATGGA CGAGCTGTAC AAGGGTGGAG GTACCACTAG TGATGAGGAG GAGAATAGCT
  421 CCAGCTCCTC CCAGCTAAAT AGTAACACCC GCCCCAGCTC TGCTACTAGC AGGAAGTCCG
  481 TCAGGGAGGC AGCCTCAGCC CCTAGCCCAA CAGCTCCAGA GCAACCAGTG GACGTTGAGG
  541 TCCAGGATCT TGAGGAGTTT GCACTGAGGC CGGCCCCCCA GGGTATCACC ATCAAATGCC
  601 GCATCACTCG GGACNAGAAA GGGATGGANC GGGGCATGTA CCCCACCTAC TTTCTGCACC
  661 TGNANCGTGA NGNNGGGNNN ANNNGNTCNT NCTNNNGGNN NNNNNANNNN NNNNNTAAAN
721 NNTNNNNNNN NNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB16766_A8Kmh.seq check
>D05982A3_A8Km_1_SV40pro_F_D09_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANNNGTGAG GANGNTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGANCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGNNCTG
  841 ANCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC NCANCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTNNTNC TGANCNGGNN NCTGGGGNNN GCGAAATGAC NNANCNANCG
961 ACGCCNNANC NNGNNNTCNC NNNNNTNNNA NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]