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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-TubbyC

Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C).

Catalog number RDB16766
Resource name pDEST/mCherry-TubbyC
Clone info. Expression vector of human Tubby proteins C-terminal tubby domain (Tubby C) fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TUB cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
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External Database
human TUB

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012) is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012)の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB16766 pDEST/mCherry-TubbyC DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16766_A8Kmp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Red-C_F
          Sequence file: RDB16766_A8Kma.seq check
          >D05982A3_A8Km_1_Red-C_F_C09_09_ABI24.ab1
              1 CAACAAATCA GTTGGACTCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA
             61 ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGG GTGGAGGTAC
            121 CACTAGTGAT GAGGAGGAGA ATAGCTCCAG CTCCTCCCAG CTAAATAGTA ACACCCGCCC
            181 CAGCTCTGCT ACTAGCAGGA AGTCCGTCAG GGAGGCAGCC TCAGCCCCTA GCCCAACAGC
            241 TCCAGAGCAA CCAGTGGACG TTGAGGTCCA GGATCTTGAG GAGTTTGCAC TGAGGCCGGC
            301 CCCCCAGGGT ATCACCATCA AATGCCGCAT CACTCGGGAC AAGAAAGGGA TGGACCGGGG
            361 CATGTACCCC ACCTACTTTC TGCACCTGGA CCGTGAGGAT GGGAAGAAGG TGTTCCTCCT
            421 GGCGGGAAGG AAGAGAAAGA AGAGTAAAAC TTCCAATTAC CTCATCTCTG TGGACCCAAC
            481 AGACTTGTCT CGAGGAGGGG ACAGCTATAT CGGGAAACTG CGGTCCAACT TGATGGGCAC
            541 CAAGTTCACT GTTTATGACA ATGGAGTCAA CCCTCAGAAG GCCTCATCCT CCACTTTGGA
            601 AAGTGGAACC TTACGTCAGG AGCTGGCAGC TGTGTGCTAC GAGACAAACG TCTTAGGCTT
            661 CAAGGGGCCT CGGAAGATGA GCGTGATTGT CCCAGGCATG AACATGGTTC ATGAGAGAGT
            721 CTCTATCCGC CCCCGCAACG AGCATGAGAC ACTGCTAGCA CGCTGGCAGA ATAAGAACAC
            781 GGAGAGTATC ATCGAGCTGC AAAACAAGAC ACCTGTCTGG AATGATGACA CACAGTCCTA
            841 TGTACTCAAC TTCCATGGGC GCGTCACACA GGCCTCCGTG AAGAACTTCC AGATCATCCA
            901 TGGCAATGAC CCGGACTACA TCGTGATGCA GTTTGGCCGG GTAGCAGAGG ATGTGTTCAC
            961 CATGGATTAC AACTACCCGC TGTGTGCACT GCAGGCCTTT GCCCATTGCC CTGTCCAGCT
           1021 TCGACAGCAA GCTGGGCGTG CGAGTAGAGA TCCGTTTAAA CCCCGCTGAA TCAGCCTCGA
           1081 CCTGTGCCAT TCTATTGCCA GGCCATCTGC TGACTTTGGC CCCGTCCCCC CGATGCCATT
          1141 CCATTTGGAA GCATG
          //
          Primer: F1ori_F
          Sequence file: RDB16766_A8Kmb.seq check
          >D05982A3_A8Km_1_f1ori_F_C11_03_ABI08.ab1
              1 GCGGGGCCCC TGGGGGCGCT GGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC
             61 CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTGGGGATAC CCCCTAGAGC CCCAGCTGGT
            121 TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC
            181 CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT
            241 CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC
            301 CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
            361 AGTCGAGGCT GATCAGCGGG TTTAAACGGA TCTCTACTCG CACGCCAGCT TGCTGTCGAA
            421 GCTGGACAGG GCAATGGCAA AGGCCTGCAG TGCACACAGC GGGTAGTTGT AATCCATGGT
            481 GAACACATCC TCTGCTACCC GGCCAAACTG CATCACGATG TAGTCCGGGT CATTGCCATG
            541 GATGATCTGG AAGTTCTTCA CGGAGGCCTG TGTGACGCGC CCATGGAAGT TGAGTACATA
            601 GGACTGTGTG TCATCATTCC AGACAGGTGT CTTGTTTTGC AGCTCGATGA TACTCTCCGT
            661 GTTCTTATTC TGCCAGCGTG CTAGCAGTGT CTCATGCTCG TTGCGGGGGC GGATAGAGAC
            721 TCTCTCATGA ACCATGTTCA TGCCTGGGAC AATCACGCTC ATCTTCCGAG GCCCCTTGAA
            781 GCCTAAGACG TTTGTCTCGT AGCACACAGC TGCCAGCTCC TGACGTAAGG TTCCACTTTC
            841 CAAGTGGAGG ATGAGGCCTT CTGAGGGTTG ACTCCATTGT CATAAACAGT GAACTTGGTG
            901 CCCATCAAGT TGGACCGCAG TTTCCCGATA TAGCTGTCCC CTCCTCGAGA CAAGTCTGTT
            961 GGGTCCACAG AGATGAGGTA ATTTGGAAGT TTTACTCTTC TTTCTCTTCC TTCCCGCCAG
           1021 GGAGGAACAC CTTTCTTCCC ATCCTCACGG TCCAAGGTGC AGAAAGTAGT GGGGTACATG
           1081 CCCCCGGGTC CATCCCTTTT CTTGTCCCGG AGTGATGCGG CATTGATGGT TGATACCTGT
          1141 GGGACCGACC TCAGATGCAA ACCTCCCTTC AAAGAATTCC T
          //
          Primer: CMV_Forward
          Sequence file: RDB16766_A8Kmc.seq check
          >D05982A3_A8Km_1_CMV_Forward_A09_03_ABI24.ab1
              1 TGGATCCACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA TCAGGTACCA TGGTGAGCAA
            181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
            241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
            301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA
            361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT
            421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
            481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT
            541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA
            601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG
            661 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC
            721 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT
            781 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG
            841 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGT GGAGGTACCA CTAGTGATGA
            901 GGAGGAGAAT AGCTCCAGCT CCTCCCAGCT AAATAGTAAC ACCCGCCCCA GCTCTGCTAC
            961 TAGCAGGAAG TCCGTCAGGA GGCAGCCCTC AGCCCCTAGC CCAACAGCTC CAGAGCAACC
           1021 AGTGGACGTT TGAGGGTCCA GGATCTTGAG AGTTTTGCAC TGAGCCCGGG CCCCCCCACG
           1081 TATCACCATC AATGCCGCAT CACTCGGACA AGAAGGTATG GGAACCGGGG CAATCTACCC
          1141 CCCCACCTTA CCTATC
          //
          Primer: SV40pro_F
          Sequence file: RDB16766_A8Kmd.seq check
          >D05982A3_A8Km_1_SV40pro_F_D09_12_ABI24.ab1
              1 CCCTTGGTCC TTGGCTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
            781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
            841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
            901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG
            961 ACGCCCAACC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCCTTCTAT GAAAGGTTGG
           1021 GCTTCGGAAT CGTTTTCCGG GACGCCGGGC TGGGATGATC CTCCAGCGCG GGGATCTCAT
           1081 GCTGCAGGTT CTTTCGCCCA CCCCGACTGG TTAATGGCAG CTTATATGTT TAACAAATAA
          1141 CGGCCAATAA GCAATTCACA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl