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pDEST/mCherry-Rab18 S22N

Expression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.

Catalog number RDB14701
Resource name pDEST/mCherry-Rab18 S22N
Clone info. Expression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
Other clones in our bank

External Database
human RAB18

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14701 pDEST/mCherry-Rab18 S22N DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14701_A7Djp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mCherry-C_F
          Sequence file: RDB14701_A7Dja.seq check
          >D04483A5_A7Dj_1_mCherry-C_F_A06_03_ABI24.ab1
              1 GAGAAAGGGA GGCCCGGTGG CCGGCTGCCC GGGCGCCTAC AACGTCAACA TCAAGTTGGA
             61 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG
            121 CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGAAGCTT ACAAGTTTGT ACAAAAAAGC
            181 AGGCTCCGCG GCCGCCTTGT TTAACTTTAA GAAGGAGCCC TTCACCATGG ACGAGGACGT
            241 GCTAACCACC CTGAAGATCC TCATCATCGG CGAGAGTGGG GTGGGCAAGA ATTCCCTGCT
            301 CTTGAGGTTC ACAGATGATA CGTTTGATCC AGAACTTGCA GCAACAATAG GTGTTGACTT
            361 TAAGGTGAAA ACAATTTCAG TGGATGGAAA TAAGGCTAAA CTTGCAATAT GGGATACTGC
            421 TGGTCAAGAG AGGTTTAGAA CATTAACTCC CAGCTATTAT AGAGGTGCAC AGGGTGTTAT
            481 ATTAGTTTAT GATGTCACAA GAAGAGATAC ATTTGTTAAA CTGGATAATT GGTTAAATGA
            541 ATTGGAAACA TACTGTACAA GAAATGACAT AGTAAACATG CTAGTTGGAA ATAAAATCGA
            601 TAAGGAAAAT CGTGAAGTCG ATAGAAATGA AGGCCTGAAA TTTGCACGAA AGCATTCCAT
            661 GTTATTTATA GAGGCAAGTG CAAAAACCTG TGATGGTGTA CAATGTGCCT TTGAAGAACT
            721 TGTTGAAAAG ATCATTCAGA CCCCTGGACT GTGGGAAAGT GAGAACCAGA ATAAAAGGAG
            781 TCAAAACTGT CACACAGGGA AGAAAGGCCA AGGAGGAGGA GCCTGTGGTG GTTATTGCTC
            841 TGTGTTATAA AAGGGTGGGC GCGCCGACCC AGCTTTTCTT GTAACAAAGT GGTTGATCTA
            901 GAGGGCCCGC GGTTCGAAGG TAAGCCTATC CCTACCCTCT CCTCGGTCTC GAATTCTACG
            961 CGTACCGGTC ATCATCACCA TCACCATTGA GTTTAAAAAC CCGCCTGATC AGCCTCGACT
           1021 GTGCCTTCCT AGTTGCCAGC CCATCTGTTG TTTGCCCCTT CCCCCCGGTG CCCTCTGGAC
           1081 CTGGAAAGTG CCACTTCCCA CTGTCCTTCT AATAATGAGG AAAACTGCAT CGCATTGTCT
          1141 GATTAGGGTG TCCAATTCTC TATTCTGGGG GGTGGTCGGC GCTGGGGACT AGAGA
          //
          Primer: BGH_rev2
          Sequence file: RDB14701_A7Djb.seq check
          >D04483A5_A7Dj_1_BGH_rev2_B06_06_ABI24.ab1
              1 CTCCACCCAT GGTCGAAGTC AAGCTGGGGG GCAGGGCAAA CAGCAGATGG CGTGGCAACT
             61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC
            121 GGTACGCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG
            181 GCCCTCTAGA TCAACCACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC CCTTTTATAA
            241 CACAGAGCAA TAACCACCAC AGGCTCCTCC TCCTTGGCCT TCTTCCCTGT GTGACAGTTT
            301 GACTCCTTTA TTCTGGTTCT CACTTTCCCA CAGTCCAGGG GTCTGAATGA TCTTTTCAAC
            361 AAGTTCTTCA AAGGCACATT GTACACCATC ACAGGTTTTT GCACTTGCCT CTATAAATAA
            421 CATGGAATGC TTTCGTGCAA ATTTCAGGCC TTCATTTCTA TCGACTTCAC GATTTTCCTT
            481 ATCGATTTTA TTTCCAACTA GCATGTTTAC TATGTCATTT CTTGTACAGT ATGTTTCCAA
            541 TTCATTTAAC CAATTATCCA GTTTAACAAA TGTATCTCTT CTTGTGACAT CATAAACTAA
            601 TATAACACCC TGTGCACCTC TATAATAGCT GGGAGTTAAT GTTCTAAACC TCTCTTGACC
            661 AGCAGTATCC CATATTGCAA GTTTAGCCTT ATTTCCATCC ACTGAAATTG TTTTCACCTT
            721 AAAGTCAACA CCTATTGTTG CTGCAAGTTC TGGATCAAAA CGTATCATCT GTGAACCTCA
            781 AAGAGCAGGG AATTCTTGCC CACCCCACTC TCGCCGATGA TGAGGATCTT CAGGGTGGTT
            841 AGCACGTCCT CGTCCATGGT GAAGGGCTCC TTCTTAAAGT TAAACAAGGC GGCCCGCGGA
            901 GCCTGCTTTT TTTGTACAAA CTTGTAAGCT TCTTGTAACA GCTCGTCCAT GCCCGCCGGT
            961 GGAGTGGGCG GGCCCTCGGC GCGTTTCGTA CTGTTTCCAC GAATGGTGTA GTCCTCGTTG
           1021 TGGGAGGTGA TGGTCCCAAC CTTGATGTTG ACGTTGGTAG GCGCCGGGGC AAGCCTGCAC
           1081 GGGCTTCTTT GGCCTTGTAA GGTGGGGTCC TTTGACCCTT CAGCGTTCGT AGTGGCCCGC
           1141 CCGTCCCTTC AGCCTCAGCC CTCTGCTTGG ATTCCTCGCC TTCAAGGGGC CGGCCGTCCT
          1201 CCTGGGGGGT AATACACACA
          //
          Primer: CMV-Forward
          Sequence file: RDB14701_A7Djc.seq check
          >D04483A5_A7Dj_1_CMV-Forward_C06_09_ABI24.ab1
              1 ATGATACCAC CTTCCGGCCC CCATTTGGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA
             61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
            121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTATC AGGTACCATG
            181 GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG
            241 CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC
            301 CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC
            361 GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC
            421 GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG
            481 ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC
            541 GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG
            601 CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC
            661 CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG
            721 GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC
            781 ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC
            841 GCCGAGGGGC CGCCACTCCA CCGGGCGGGC ATGGACGAGC TGTACAAGGA AGCTTACAAG
            901 TTTGTACAAA AAAGCAGGCT CCGCGGGCCG CCTTGTTTAA CTTTAAGAAG GGAGCCCTTC
            961 ACCATGGGAC GAGGGACGTG CTAACCACCC TGAAGATCCT CATCATCGGG CGAGAGTGGG
           1021 GGTGGGCAAG AATTCCCTGG CTCTTGAGGG TTCACAGAAT GATACCGTTT GATCCAGAAC
           1081 CTGCAGCAAC CAATAGGTGT TGAACTTTAA AGGTGGAAAC AATTTTCAGT GGGATGGAAA
           1141 TAGGCTTAAC CTTGGCCAAT AATGGGGGAA TTACCCTGTG CCCTGGGTCT CCATGAGGAA
          1201 G
          //
          Primer: SV40pro_F
          Sequence file: RDB14701_A7Djd.seq check
          >D04483A5_A7Dj_1_SV40pro_F_D06_12_ABI24.ab1
              1 GCCTGCGGGC CTGGGACCTT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
             61 TTGCAAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT
            121 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
            181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
            241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
            301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
            361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
            421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
            481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
            541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
            601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
            661 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
            721 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG
            781 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT
            841 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT
            901 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGGACTCT GGGGGTTCGC GAAATGACCG
            961 ACCAAGCGAC GCCCAACCTG CCCATCACGA GATTTCGATT CCACCGCCCG CCCTTCTATG
           1021 AAAGGTTGGG GCTTCGGAAT CGTTTTCCCG GACGCCCGGC TGGGATGAAT CTCAGCGCGG
           1081 GGATCTCATG CTGGAGTTCT TCGCCACCCC CAACTTGGTA ATGCAGCCTA TAATGGGTTA
          1141 CAAATGAGCC ATTAGGCCAT GCACACAAAA TTTACA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl