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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-Rab18 S22N (#RDB14701)

Expression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.

Clone info. Expression vector of human Rab18, dominant-negative GDP-binding mutant (S22N), fused with mCherry.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pDEST-mCherry (plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human RAB18 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al. Mol. Biol. Cell, 27(21): 3293-3304, 2016)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14701 pDEST/mCherry-Rab18 S22N DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-Rab18 S22N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14701).

Reference section:

Makino, A., Hullin-Matsuda, F., Murate, M., Abe, M., Tomishige, N., Fukuda, M., Yamashita, S., Fujimoto, T., Vidal, H., Lagarde, M., Delton, I., Kobayashi, T., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14701_A7Djp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F ()
Sequence file: RDB14701_A7Dja.seq check
>D04483A5_A7Dj_1_mCherry-C_F_A06_03_ABI24.ab1
    1 GAGAAAGGGA GGCCCGGTGG CCGGCTGCCC GGGCGCCTAC AACGTCAACA TCAAGTTGGA
   61 CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG
  121 CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGAAGCTT ACAAGTTTGT ACAAAAAAGC
  181 AGGCTCCGCG GCCGCCTTGT TTAACTTTAA GAAGGAGCCC TTCACCATGG ACGAGGACGT
  241 GCTAACCACC CTGAAGATCC TCATCATCGG CGAGAGTGGG GTGGGCAAGA ATTCCCTGCT
  301 CTTGAGGTTC ACAGATGATA CGTTTGATCC AGAACTTGCA GCAACAATAG GTGTTGACTT
  361 TAAGGTGAAA ACAATTTCAG TGGATGGAAA TAAGGCTAAA CTTGCAATAT GGGATACTGC
  421 TGGTCAAGAG AGGTTTAGAA CATTAACTCC CAGCTATTAT AGAGGTGCAC AGGGTGTTAT
  481 ATTAGTTTAT GATGTCACAA GAAGAGATAC ATTTGTTAAA CTGGATAATT GGTTAAATGA
  541 ATTGGAAACA TACTGTACAA GAAATGACAT AGTAAACATG CTAGTTGGAA ATAAAATCGA
  601 TAAGGAAAAT CGTGAAGTCG ATAGAAATGA AGGCCTGAAA TTTGCACGAA AGCATTCCAT
  661 GTTATTTATA GAGGCAAGTG CAAAAACCTG TGATGGTGTA CAATGTGCCT TTGAAGAACT
  721 TGTTGAAAAG ATCATTCAGA CCCCTGGACT GTGGGAAAGT GAGAACCAGA ATAAAAGGAG
  781 TCAAAACTGT CACACAGGGA AGAAAGGCCA AGGAGGAGGA GCCTGTGGTG GTTATTGCTC
  841 TGTGTTATAA AAGGGTGGGC GCGCCGACCC AGCTTTTCTT GTAACAAAGT GGTTGATCTA
  901 GAGGGCCCGC GGTTCGAAGG TAAGCCTATC CCTACCCTCT CCTCGGTCTC GAATTCTACG
  961 CGTACCGGTC ATCATCACCA TCACCATTGA GTTTAAAAAC CCGCCTGATC AGCCTCGACT
 1021 GTGCCTTCCT AGTTGCCAGC CCATCTGTTG TTTGCCCCTT CCCCCCGGTG CCCTCTGGAC
 1081 CTGGAAAGTG CCACTTCCCA CTGTCCTTCT AATAATGAGG AAAACTGCAT CGCATTGTCT
1141 GATTAGGGTG TCCAATTCTC TATTCTGGGG GGTGGTCGGC GCTGGGGACT AGAGA
//
Primer: BGH_rev2 ()
Sequence file: RDB14701_A7Djb.seq check
>D04483A5_A7Dj_1_BGH_rev2_B06_06_ABI24.ab1
    1 CTCCACCCAT GGTCGAAGTC AAGCTGGGGG GCAGGGCAAA CAGCAGATGG CGTGGCAACT
   61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC
  121 GGTACGCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG
  181 GCCCTCTAGA TCAACCACTT TGTACAAGAA AGCTGGGTCG GCGCGCCCAC CCTTTTATAA
  241 CACAGAGCAA TAACCACCAC AGGCTCCTCC TCCTTGGCCT TCTTCCCTGT GTGACAGTTT
  301 GACTCCTTTA TTCTGGTTCT CACTTTCCCA CAGTCCAGGG GTCTGAATGA TCTTTTCAAC
  361 AAGTTCTTCA AAGGCACATT GTACACCATC ACAGGTTTTT GCACTTGCCT CTATAAATAA
  421 CATGGAATGC TTTCGTGCAA ATTTCAGGCC TTCATTTCTA TCGACTTCAC GATTTTCCTT
  481 ATCGATTTTA TTTCCAACTA GCATGTTTAC TATGTCATTT CTTGTACAGT ATGTTTCCAA
  541 TTCATTTAAC CAATTATCCA GTTTAACAAA TGTATCTCTT CTTGTGACAT CATAAACTAA
  601 TATAACACCC TGTGCACCTC TATAATAGCT GGGAGTTAAT GTTCTAAACC TCTCTTGACC
  661 AGCAGTATCC CATATTGCAA GTTTAGCCTT ATTTCCATCC ACTGAAATTG TTTTCACCTT
  721 AAAGTCAACA CCTATTGTTG CTGCAAGTTC TGGATCAAAA CGTATCATCT GTGAACCTCA
  781 AAGAGCAGGG AATTCTTGCC CACCCCACTC TCGCCGATGA TGAGGATCTT CAGGGTGGTT
  841 AGCACGTCCT CGTCCATGGT GAAGGGCTCC TTCTTAAAGT TAAACAAGGC GGCCCGCGGA
  901 GCCTGCTTTT TTTGTACAAA CTTGTAAGCT TCTTGTAACA GCTCGTCCAT GCCCGCCGGT
  961 GGAGTGGGCG GGCCCTCGGC GCGTTTCGTA CTGTTTCCAC GAATGGTGTA GTCCTCGTTG
 1021 TGGGAGGTGA TGGTCCCAAC CTTGATGTTG ACGTTGGTAG GCGCCGGGGC AAGCCTGCAC
 1081 GGGCTTCTTT GGCCTTGTAA GGTGGGGTCC TTTGACCCTT CAGCGTTCGT AGTGGCCCGC
 1141 CCGTCCCTTC AGCCTCAGCC CTCTGCTTGG ATTCCTCGCC TTCAAGGGGC CGGCCGTCCT
1201 CCTGGGGGGT AATACACACA
//
Primer: CMV-Forward ()
Sequence file: RDB14701_A7Djc.seq check
>D04483A5_A7Dj_1_CMV-Forward_C06_09_ABI24.ab1
    1 ATGATACCAC CTTCCGGCCC CCATTTGGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA
   61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
  121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTATC AGGTACCATG
  181 GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG
  241 CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC
  301 CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC
  361 GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC
  421 GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG
  481 ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC
  541 GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG
  601 CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC
  661 CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG
  721 GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC
  781 ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC
  841 GCCGAGGGGC CGCCACTCCA CCGGGCGGGC ATGGACGAGC TGTACAAGGA AGCTTACAAG
  901 TTTGTACAAA AAAGCAGGCT CCGCGGGCCG CCTTGTTTAA CTTTAAGAAG GGAGCCCTTC
  961 ACCATGGGAC GAGGGACGTG CTAACCACCC TGAAGATCCT CATCATCGGG CGAGAGTGGG
 1021 GGTGGGCAAG AATTCCCTGG CTCTTGAGGG TTCACAGAAT GATACCGTTT GATCCAGAAC
 1081 CTGCAGCAAC CAATAGGTGT TGAACTTTAA AGGTGGAAAC AATTTTCAGT GGGATGGAAA
 1141 TAGGCTTAAC CTTGGCCAAT AATGGGGGAA TTACCCTGTG CCCTGGGTCT CCATGAGGAA
1201 G
//
Primer: SV40pro_F ()
Sequence file: RDB14701_A7Djd.seq check
>D04483A5_A7Dj_1_SV40pro_F_D06_12_ABI24.ab1
    1 GCCTGCGGGC CTGGGACCTT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
   61 TTGCAAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT
  121 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
  181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
  241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
  301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
  361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
  421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
  481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
  541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
  601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
  661 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
  721 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG
  781 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT
  841 GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT
  901 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGGACTCT GGGGGTTCGC GAAATGACCG
  961 ACCAAGCGAC GCCCAACCTG CCCATCACGA GATTTCGATT CCACCGCCCG CCCTTCTATG
 1021 AAAGGTTGGG GCTTCGGAAT CGTTTTCCCG GACGCCCGGC TGGGATGAAT CTCAGCGCGG
 1081 GGATCTCATG CTGGAGTTCT TCGCCACCCC CAACTTGGTA ATGCAGCCTA TAATGGGTTA
1141 CAAATGAGCC ATTAGGCCAT GCACACAAAA TTTACA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makino, A., Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol. Biol. Cell. 27 (21): 3293-3304 (2016). PMID 27582390. [link to RRC of NBRP]