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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-PIP5KB (#RDB16765)

Expression vector of human phosphatidylinositol 4-phosphate 5-kinase beta (PIP5K beta).

Clone info. Expression vector of human phosphatidylinositol 4-phosphate 5-kinase beta (PIP5K beta) fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PIP5K1B cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

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Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012）の引用を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16765 pDEST/mCherry-PIP5KB DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-PIP5KB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16765).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16765_A8Kmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Red-C_F ()
Sequence file: RDB16765_A8Kma.seq check
>D05982A2_A8Km_2_Red-C_F_G08_20_ABI24.ab1
    1 CTGCCACTCA GTTGGAATCA CCTCCCACAA CGAGGACTAC ACCATCGTGG AACAGTACGA
   61 ACGCGCCGAG GGCCGCCACT CCACCGGCGG CATGGACGAG CTGTACAAGG GAGGTGGAGG
  121 TTCTAGAATG TCTTCTGCTG CTGAAAATGG AGAGGCAGCA CCTGGAAAAC AAAATGAAGA
  181 AAAAACCTAT AAAAAGACTG CATCATCTGC TATTAAAGGT GCTATTCAGC TGGGAATAGG
  241 ATACACAGTG GGTAATCTCA CTTCCAAGCC AGAACGAGAT GTTCTTATGC AAGACTTTTA
  301 TGTGGTGGAA AGTGTGTTCC TACCCAGCGA AGGGAGCAAT CTGACCCCAG CACATCACTA
  361 CCCAGACTTT AGATTTAAGA CATACGCTCC ATTAGCATTC CGATATTTCA GAGAACTTTT
  421 TGGTATCAAG CCTGATGATT ACTTGTATTC CATCTGCAGT GAACCTCTAA TAGAACTGTC
  481 TAACCCTGGA GCCAGTGGAT CCTTGTTTTT TGTGACCAGT GATGATGAAT TTATCATCAA
  541 AACAGTTCAG CACAAAGAAG CTGAGTTTCT TCAGAAGCTA CTGCCAGGCT ATTACATGAA
  601 TTTAAACCAG AATCCAAGGA CTCTTTTGCC AAAATTTTAC GGACTGTATT GTATGCAATC
  661 AGGAGGCATT AATATCAGGA TTGTGGTGAT GAACAACGTT TTGCCACGCT CCATGAGAAT
  721 GCACTTTACA TATGACTTGA AAGGCTCAAC GTATAAGCGA AGAGCATCCC GTAAAGAGAG
  781 AGAGAAATCC AACCCCACAT TTAAGGACTT AGATTTCCTG CAAGACATGC ACGAAGGGTT
  841 GTATTTTGAT ACGGAAACAT ACAACGCGCT TATGAAAACA CTTCAGAGAG ACTGCCGGGT
  901 GCTAGAAAGC TTCAAGATCA TGGATTATAG CCTTCTGTTG GGAATTCATT TCCTGGACCA
  961 TTCCCTCAAA GAGAAAGAGG AGGAGACCCC ACAAAATGTG CCCTGATGCT AAGCGGGACT
 1021 GGGATGCAGA AGGTTCTTCT ACTCAACAGC CATGGCAATC TATCCAGGGT CCAGGGAATC
 1081 TGGAGATGGG ATAATCACAG AGCACCCAGA CGCAATGGCA GCAGTTCAGC CTTAAAAGCC
1141 AATAGGGGGG AGAAAAAATC T
//
Primer: F1ori_F ()
Sequence file: RDB16765_A8Kmb.seq check
>D05982A2_A8Km_2_f1ori_F_B11_02_ABI08.ab1
    1 GAGGGGCCGT TAGGGCGCTG GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC
   61 GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TGGGGATACC CCCTAGAGCC CCAGCTGGTT
  121 CTTTCCGCCT CAGAAGCCAT AGAGCCCACC GCATCCCCAG CATGCCTGCT ATTGTCTTCC
  181 CAATCCTCCC CCTTGCTGTC CTGCCCCACC CCACCCCCCA GAATAGAATG ACACCTACTC
  241 AGACAATGCG ATGCAATTTC CTCATTTTAT TAGGAAAGGA CAGTGGGAGT GGCACCTTCC
  301 AGGGTCAAGG AAGGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA
  361 GTCGAGGCTG ATCAGCGGGT TTAAACCTCG AGTTATAAAT AGACGTCAAG CACAGAAGCA
  421 TTGTCATCCT GCACTTCCAG AGTGTTGGGC TCAGCGGTCA AGTAGATGGT CCCCTCTTCC
  481 AAGGTAAATG TTGAACTGGA AGGTAACCCT TTGCTGTTTG AATAGAGTGT AGGCCTGTCG
  541 TGTGGATAGT GCTCATTGAC GTATAAGGAA GAAGATGAAA TTGTGGTTGC CAAGGAAGCA
  601 GCTTCAAACA GTGATGGAGT GCTAGGGACC AGGTCTGGCC TGTGTCGGGA TCCCAGGGCT
  661 TCTTCATCAA GGCTGCTAAA ACTTTGTCCT TCAGTCAGCA AATCCCGCTT CTCATCCTTC
  721 CATTCTTGGC TAATTGAGGA CACAATCTCC TGTGAAGTGG CCTTTAGGGC GGCGATTGAA
  781 TTGCACCGTT TCTTAGACGG TGAAGCCTTC AAAGCTTGAA TTTTCTTGAA AACTCTGGAA
  841 TTCATGAACT TAAGAAATCT GTCTGCATAA AAGCTTGGTC TATGAACAGA AACAGTGTCC
  901 CCATCATAAA CAAGAGCTTT CCAGGAAATG TTCTAACTTC TTCATTAACC TATATGATTG
  961 CAGAATGTCA TAATGCCCAT AAATAAAAGT AGTTTTTTCT CCCCTATGGC TTTTAGCTGG
 1021 AATGCCCTCC CATTGTGTCT GGGATTCTCT GTGATTATCC CATCTCCAGA TTCCCTGGAC
 1081 CTGGATAGGA TTTCATGGCT GTGGAGTAGA GAACATCTGC ATTCCAGTCG CTTAGCATCA
1141 AGGCACATTT GGTTGGGGTC TCCTTTCCTT TCCT
//
Primer: CMV_Forward ()
Sequence file: RDB16765_A8Kmc.seq check
>D05982A2_A8Km_2_CMV_Forward_E08_14_ABI24.ab1
    1 TGAACACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GTTAAGCTAT CAGGTACCAT GGTGAGCAAG
  181 GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG
  241 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG
  301 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC
  361 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC
  421 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC
  481 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC
  541 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG
  601 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC
  661 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC
  721 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG
  781 GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC
  841 CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGGGAG GTGGAGGTTC TAGAATGTCT
  901 TCTGCTGCTG AAAATGGAGA GGCAGCACCT GGAAAACAAA ATGAAGAAAA AACCTATAAA
  961 AAGACTGCAT CATCTGCTAT TAAAGGTGCT ATTCAGCTGG GAATAGGATA CACAGTGGGT
 1021 AATCTCACTT CCAAGCCCAG AACGAGATGT TTCTTATGCA GACTTTTTTA TGTGGAGAAA
 1081 GTGTGTTCCT ACCCCAGCGA AGAAGCCATC CTGGACCCCA GCCACATTCA ACTACCCCAG
1141 AAACCTTTTA GGAAATTTTA AGG
//
Primer: SV40pro_F ()
Sequence file: RDB16765_A8Kmd.seq check
>D05982A2_A8Km_2_SV40pro_F_H08_23_ABI24.ab1
    1 CCCTTGGTCC TGACCTTTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTTC GCGAAATGAC CGACCAAGCG
  961 ACGCCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCG CTTCTATGAA GTTGGGCTTC
 1021 GGATCGTTTT TCGGGACGCG GCTGATGATC CTCAAGCGCG GGGATCTCAT GCCTGGAAGT
1081 CTCGCCTACT CAACTTGGTT AATGCCAGCT AATAATTGGT TTAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]