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pGEX-GFP-ERK2-His

Bacterial expression vector of GFP–human ERK2.

Catalog number RDB14152
Resource name pGEX-GFP-ERK2-His
Alternative name GL 749
Clone info. Bacterial expression vector of GFP–human ERK2.
Comment A fragment of GFP–human ERK2 is introduced into pGEX2T which is a GST fusion protein expression vector, and then an N-terminal PreScission site and C-terminal 8His-tag are introduced. Expression was confirmed by the depositor with Phos-tag western blotting.
Vector backbone pGEX2T (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human ERK2 cDNA
Depositor|Developer Sako, Yasushi |
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External Database
human ERK2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14152 pGEX-GFP-ERK2-His DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14152_A6Ecp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-N
          Sequence file: RDB14152_A6Eca.seq check
          >D03441A1_A6Ec_2_EGFP_N_A04_01.ab1
              1 GAACCGGGGG GGAGGCTCTC GCCTTGCTCA CCATCCCGGG ACCCTGGAAC AGAACTTCCA
             61 GAGATCCACG CGGAACCAGA TCCGATTTTG GAGGATGGTC GCCACCACCA AACGTGGCTT
            121 GCCAGCCCTG CAAAGGCCAT GCTATATACT TGCTGGATTT CAAGTACTTA TCAATTTGTG
            181 GGATAGCTTC AATACGTTTT TTAAAACAAA CTAATTTTGG GAACGCATCC AGGCACATTG
            241 GGTCCATGTA TAAAACAACA TCAAGAGCGT CATACAACAT GAAGTCAGGA TGGGTTACAT
            301 GATCACCATT TAAATATGTT TTATGACATA AACGATCTTC GAACATTTTC AGCATTTCAG
            361 GTAGCTTGCT AAGAAAATCA ACTTTGAGAG TTTCAAAGTC TTTACTATAT GCAATTCTCG
            421 AAACACCGTA TCTAATATCC AAAACCGCTC CTTCAAGCAT TGAAATCTCT GCACGCTCTT
            481 TTGGACAACC ACCCAACATG TTGTGCTTGT CAGCTATATA ACGTATGATG GCCATAGACT
            541 GTGTTAATTT AACATCACCA TCAATATAAT AAGGAAGATT GGGAAACTCC AAACCCAATT
            601 CAAACTTTTT GTTTCGCCAT TTATCACCTT CATCGCGCTC ATACAAATGC TCTTCATATT
            661 TTTCTTCAAG ATATTCCAAA AGAAGTCGAG TGGGTTGCAC AAGGCCCTTA ATTTTCCAAT
            721 AACCTAGTAT AGGGGACATG AATACTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT
            781 CCACACATTA TACGAGCCGA TGATTAATTG TCAACAGCTC ATTTCAGAAT ATTTGCCAGA
            841 ACCGTTATGA TGTCGGCGCA AAAAACATTA TCCAGAACGG GAGTGCGCCT TGAGCGACAC
            901 GAATTATGCA GTGATTTACG ACCTGCACAG CCATACCACA GCTTCCGATG GGCTGCCTGA
            961 CGCCAGAAGC ATTTGGTGCC ACCGTGCAGT CGATAAGCTT ATACGACGCA TCAAAATTAT
          1021 CGCGTCTGGG CTTCCTTGTA GACAGCCTTC CATTCGACAT TAAAATGTGA AGCCGA
          //
          Primer: EGFP-C
          Sequence file: RDB14152_A6Ecb.seq check
          >D03441A1_A6Ec_2_EGFP_C_B04_04.ab1
              1 CAGGTATCTC GGGCATGGAC GAGCTGTACA AGTCCGGCCG GACTCAGATC CAAGCTTCGA
             61 ATTCTGCAGT CGACGGTACC GCGGGCCCAT CAACAAGTTT GTACAAAAAA GCAGGCTTTA
            121 TCCCGGCAGC CCATATGGCG GCGGCGGCGG CGGCGGGCGC GGGCCCGGAG ATGGTCCGCG
            181 GGCAGGTGTT CGACGTGGGG CCGCGCTACA CCAACCTCTC GTACATCGGC GAGGGCGCCT
            241 ACGGCATGGT GTGCTCTGCT TATGATAATG TCAACAAAGT TCGAGTAGCT ATCAAGAAAA
            301 TCAGCCCCTT TGAGCACCAG ACCTACTGCC AGAGAACCCT GAGGGAGATA AAAATCTTAC
            361 TGCGCTTCAG ACATGAGAAC ATCATTGGAA TCAATGACAT TATTCGAGCA CCAACCATCG
            421 AGCAAATGAA AGATGTATAT ATAGTACAGG ACCTCATGGA AACAGATCTT TACAAGCTCT
            481 TGAAGACACA ACACCTCAGC AATGACCATA TCTGCTATTT TCTCTACCAG ATCCTCAGAG
            541 GGTTAAAATA TATCCATTCA GCTAACGTTC TGCACCGTGA CCTCAAGCCT TCCAACCTGC
            601 TGCTCAACAC CACCTGTGAT CTCAAGATCT GTGACTTTGG CCTGGCCCGT GTTGCAGATC
            661 CAGACCATGA TCACACAGGG TTCCTGACAG AATATGTGGC CACACGTTGG TACAGGGCTC
            721 CAGAAATTAT GTTGAATTCC AAGGGCTACA CCAAGTCCAT TGATATTTGG TCTGTAGGCT
            781 GCATTCTGGC AGAAATGCTT TCTAACAGGC CCATCTTTCC AGGGAAGCAT TATCTTGACC
            841 AGCTGAACCA CATTTTTGGG TATTCTTGGA TCCCCCATCA CAAGAAAGAC CTGAAATTGT
            901 ATAAATAAAA TTTTAAAAGC TAGGAAACTA TTTTGCTTTT CTCTTTCCCA CACAAAAAAT
            961 AAAGGTGCCC ATGGAAACAG GCTGGTTTCC CAAATGCTGA CTCCCAAGCT CTGGACTTTA
           1021 TTGGACAAAA TGGATGACCA TTCCAAACCC ACACAAGCAG ATCAGTTAGA CAGGCTCTGG
          1081 GTCCGATCTC AA
          //
          Primer: pGEX-R
          Sequence file: RDB14152_A6Ecc.seq check
          >D03441A1_A6Ec_2_pGEX_R_C04_07.ab1
              1 TTTATTACGG TAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGAATTC AATGATGATG
             61 ATGATGATGA TGATGACCGG TAGCACCAGA TCTGTATCCT GGCTGGAATC TAGCAGTCTC
            121 TTCAAAAATT AGTTCTTTGA GCTTTTCCTT AGGCAAGTCA TCCAATTCCA TGTCGAACTT
            181 GAATGGTGCT TCGGCGATGG GCTCGTCACT CGGGTCGTAA TACTGCTCCA GATATGGGTG
            241 GGCCAGAGCC TGTTCTACTT CAATCCTCTT GTGTGGGTTG AATGTCAACA TTTTGTCCAA
            301 TAAGTCCAGA GCTTTGGAGT CAGCATTTGG GAACAGCCTG TTCCATGGCA CCTTATTTTT
            361 GTGTGGAAGA GAAAGCAAAT AGTTCCTAGC TTTTAAATTT ATTATACAAT TCAGGTCTTC
            421 TTGTGATGGG GATCCAAGAA TACCCAAAAT GTGGTTCAGC TGGTCAAGAT AATGCTTCCC
            481 TGGAAAGATG GGCCTGTTAG AAAGCATTTC TGCCAGAATG CAGCCTACAG ACCAAATATC
            541 AATGGACTTG GTGTAGCCCT TGGAATTCAA CATAATTTCT GGAGCCCTGT ACCAACGTGT
            601 GGCCACATAT TCTGTCAGGA ACCCTGTGTG ATCATGGTCT GGATCTGCAA CACGGGCCAG
            661 GCCAAAGTCA CAGATCTTGA GATCACAGGT GGTGTTGAGC AGCAGGTTGG AAGGCTTGAG
            721 GTCACGGTGC AGAACGTTAG CTGAATGGAT ATATTTTAAC CCTCTGAGGA TCTGGTAGAG
            781 AAAATAGCAG ATATGGTCAT TGCTGAGGTG TTGTGTCTTC AAGAGCTTGT AAAGATCTGT
            841 TTCCATGAGG TCCTGTACTA TATATACATC TTTTCATTTT GCTCGATGGT TGGTGCTCGA
            901 ATAATGTCAT TGATTCCAAT GATGTTCTCA TGTCTGAAGC GCAGTAAGAT TTTTTATCTC
            961 CCTCAGGGTT CTCTGGCAGT AGGTCTGGTG CTCAAAAGGG GCTGGATTTT TGTTGATAGC
          1021 TACCTCGCAC CTTTGATTGG GACAGTATCC ATTAAGCAGA GCCACCACCG ATTGCCCGT
          //
          Primer: SV40pA3'Element Reverse
          Sequence file: RDB14152_A6Ecd.seq check
          >D03441A1_A6Ec_2_SV40pA3'element_Reverse_D04_10.ab1
              1 GAGGTTTGGG GGGGCGGGGC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
             61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
            121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
            181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
            241 TCTGTGCGGT ATTTCACACC GCATAAATTC CGACACCATC GAATGGCGCA AAACCTTTCG
            301 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT
            361 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT
            421 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA
            481 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT
            541 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA
            601 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT
            661 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT
            721 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC
            781 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TTCTCCCATG
            841 AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG CAAATCGCGC
            901 TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGGTCTGC GTCTGGCTGG CTGGCATAAA
            961 ATATCTCACT CGCAAATCAA ATTCAGCCGA TAGCGGAACG GAAGGCGACC TGGAAGTGCA
          1021 TGTCCGGATT TTCAACAACC ATGCAAAATG CTTGAAAATG GAGGGCCCA
          //
          Primer: M13R
          Sequence file: RDB14152_A6Ece.seq check
          >D03441A1_A6Ec_2_M13R_E04_13.ab1
              1 GACGTGACGT CGTTTTTCAA CGTCGTGACT GGGAAACCCT GGCGTTACCC AACTTAATCG
             61 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG
            121 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC TTTGCCTGGT TTCCGGCACC
            181 AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT GAGGCCGATA CTGTCGTCGT
            241 CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC TACACCAACG TGACCTATCC
            301 CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG ACGGGTTGTT ACTCGCTCAC
            361 ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG CGAATTATTT TTGATGGCGT
            421 TGGAATTAGC TTATCGACTG CACGGTGCAC CAATGCTTCT GGCGTCAGGC AGCCATCGGA
            481 AGCTGTGGTA TGGCTGTGCA GGTCGTAAAT CACTGCATAA TTCGTGTCGC TCAAGGCGCA
            541 CTCCCGTTCT GGATAATGTT TTTTGCGCCG ACATCATAAC GGTTCTGGCA AATATTCTGA
            601 AATGAGCTGT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC
            661 AATTTCACAC AGGAAACAGT ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC
            721 CTTGTGCAAC CCACTCGACT TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG
            781 TATGAGCGCG ATGAAGGTGA TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT
            841 CCCAATCTTC CTTATTATAT TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCCATCAT
            901 ACGTTATATA GCTGACAGCA CAACATGTTT GGGGTGGTTG TCCCAAAAGA GCGTGCAGAG
            961 ATTTCAATGC TTTGAAGGAG CGGGTTTGGA TATTAGAATA CGTGTTTCGA GCACTGCCAT
          1021 AATAGTAAGG ACGTGAACTC CTCGAAGATT GATTCTAAGC AAGCCTAACC GAT
          //
          Primer: EGFP_N_rev
          Sequence file: RDB14152_A6Ecf.seq check
          >D03441A1_A6Ec_2_EGFP-N_rev_F04_16.ab1
              1 ACTCAAGTTC AGCGTGTCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
             61 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
            121 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
            181 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
            241 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
            301 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
            361 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
            421 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
            481 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
            541 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
            601 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGCCG GACTCAGATC
            661 CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCAT CAACAAGTTT GTACAAAAAA
            721 GCAGGCTTTA TCCCGGCAGC CCATATGGCG GCGGCGGCGG CGGCGGGCGC GGGCCCGGAG
            781 ATGGTCCGCG GGCAGGTGTT CGACGTGGGG CCGCGCTACA CCAACCTCTC GTACATCGGC
            841 GAGGGCGCCT ACGGCATGGT GTGCTCTGCT TATGATAATG TCAACAAAGT TCGAGTAGCT
            901 ATCAAGAAAA TCAGCCCCTT TGAGCACCAG ACCCTACTGC CCAGAGAACC CTGAGGAGAT
            961 AAAAATCTTA CCTGCCGCCT TCAGACATGA GACATCATGG TATCATGACA TATCGAGCAC
          1021 AACATCGAGC AATGAAAGAT GTATATATAG TTACAGGATC GC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl