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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-GFP-ERK2-His (#RDB14152)

Bacterial expression vector of GFP–human ERK2.

Alternative name GL 749
Clone info. Bacterial expression vector of GFP–human ERK2.
Comment A fragment of GFP–human ERK2 is introduced into pGEX2T which is a GST fusion protein expression vector, and then an N-terminal PreScission site and C-terminal 8His-tag are introduced. Expression was confirmed by the depositor with Phos-tag western blotting.
Vector backbone pGEX2T (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name human ERK2 cDNA
Depositor|Developer Sako, Yasushi |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14152 pGEX-GFP-ERK2-His DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-GFP-ERK2-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14152).

Reference section:

Shindo, Y., Iwamoto, K., Mouri, K., Hibino, K., Tomita, M., Kosako, H., Sako, Y., Takahashi, K., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14152_A6Ecp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N
Sequence file: RDB14152_A6Eca.seq check
>D03441A1_A6Ec_2_EGFP_N_A04_01.ab1
    1 GAACCGGGGG GGAGGCTCTC GCCTTGCTCA CCATCCCGGG ACCCTGGAAC AGAACTTCCA
   61 GAGATCCACG CGGAACCAGA TCCGATTTTG GAGGATGGTC GCCACCACCA AACGTGGCTT
  121 GCCAGCCCTG CAAAGGCCAT GCTATATACT TGCTGGATTT CAAGTACTTA TCAATTTGTG
  181 GGATAGCTTC AATACGTTTT TTAAAACAAA CTAATTTTGG GAACGCATCC AGGCACATTG
  241 GGTCCATGTA TAAAACAACA TCAAGAGCGT CATACAACAT GAAGTCAGGA TGGGTTACAT
  301 GATCACCATT TAAATATGTT TTATGACATA AACGATCTTC GAACATTTTC AGCATTTCAG
  361 GTAGCTTGCT AAGAAAATCA ACTTTGAGAG TTTCAAAGTC TTTACTATAT GCAATTCTCG
  421 AAACACCGTA TCTAATATCC AAAACCGCTC CTTCAAGCAT TGAAATCTCT GCACGCTCTT
  481 TTGGACAACC ACCCAACATG TTGTGCTTGT CAGCTATATA ACGTATGATG GCCATAGACT
  541 GTGTTAATTT AACATCACCA TCAATATAAT AAGGAAGATT GGGAAACTCC AAACCCAATT
  601 CAAACTTTTT GTTTCGCCAT TTATCACCTT CATCGCGCTC ATACAAATGC TCTTCATATT
  661 TTTCTTCAAG ATATTCCAAA AGAAGTCGAG TGGGTTGCAC AAGGCCCTTA ATTTTCCAAT
  721 AACCTAGTAT AGGGGACATG AATACTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT
  781 CCACACATTA TACGAGCCGA TGATTAATTG TCAACAGCTC ATTTCAGAAT ATTTGCCAGA
  841 ACCGTTATGA TGTCGGCGCA AAAAACATTA TCCAGAACGG GAGTGCGCCT TGAGCGACAC
  901 GAATTATGCA GTGATTTACG ACCTGCACAG CCATACCACA GCTTCCGATG GGCTGCCTGA
  961 CGCCAGAAGC ATTTGGTGCC ACCGTGCAGT CGATAAGCTT ATACGACGCA TCAAAATTAT
1021 CGCGTCTGGG CTTCCTTGTA GACAGCCTTC CATTCGACAT TAAAATGTGA AGCCGA
//
Primer: EGFP-C
Sequence file: RDB14152_A6Ecb.seq check
>D03441A1_A6Ec_2_EGFP_C_B04_04.ab1
    1 CAGGTATCTC GGGCATGGAC GAGCTGTACA AGTCCGGCCG GACTCAGATC CAAGCTTCGA
   61 ATTCTGCAGT CGACGGTACC GCGGGCCCAT CAACAAGTTT GTACAAAAAA GCAGGCTTTA
  121 TCCCGGCAGC CCATATGGCG GCGGCGGCGG CGGCGGGCGC GGGCCCGGAG ATGGTCCGCG
  181 GGCAGGTGTT CGACGTGGGG CCGCGCTACA CCAACCTCTC GTACATCGGC GAGGGCGCCT
  241 ACGGCATGGT GTGCTCTGCT TATGATAATG TCAACAAAGT TCGAGTAGCT ATCAAGAAAA
  301 TCAGCCCCTT TGAGCACCAG ACCTACTGCC AGAGAACCCT GAGGGAGATA AAAATCTTAC
  361 TGCGCTTCAG ACATGAGAAC ATCATTGGAA TCAATGACAT TATTCGAGCA CCAACCATCG
  421 AGCAAATGAA AGATGTATAT ATAGTACAGG ACCTCATGGA AACAGATCTT TACAAGCTCT
  481 TGAAGACACA ACACCTCAGC AATGACCATA TCTGCTATTT TCTCTACCAG ATCCTCAGAG
  541 GGTTAAAATA TATCCATTCA GCTAACGTTC TGCACCGTGA CCTCAAGCCT TCCAACCTGC
  601 TGCTCAACAC CACCTGTGAT CTCAAGATCT GTGACTTTGG CCTGGCCCGT GTTGCAGATC
  661 CAGACCATGA TCACACAGGG TTCCTGACAG AATATGTGGC CACACGTTGG TACAGGGCTC
  721 CAGAAATTAT GTTGAATTCC AAGGGCTACA CCAAGTCCAT TGATATTTGG TCTGTAGGCT
  781 GCATTCTGGC AGAAATGCTT TCTAACAGGC CCATCTTTCC AGGGAAGCAT TATCTTGACC
  841 AGCTGAACCA CATTTTTGGG TATTCTTGGA TCCCCCATCA CAAGAAAGAC CTGAAATTGT
  901 ATAAATAAAA TTTTAAAAGC TAGGAAACTA TTTTGCTTTT CTCTTTCCCA CACAAAAAAT
  961 AAAGGTGCCC ATGGAAACAG GCTGGTTTCC CAAATGCTGA CTCCCAAGCT CTGGACTTTA
 1021 TTGGACAAAA TGGATGACCA TTCCAAACCC ACACAAGCAG ATCAGTTAGA CAGGCTCTGG
1081 GTCCGATCTC AA
//
Primer: pGEX-R
Sequence file: RDB14152_A6Ecc.seq check
>D03441A1_A6Ec_2_pGEX_R_C04_07.ab1
    1 TTTATTACGG TAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGAATTC AATGATGATG
   61 ATGATGATGA TGATGACCGG TAGCACCAGA TCTGTATCCT GGCTGGAATC TAGCAGTCTC
  121 TTCAAAAATT AGTTCTTTGA GCTTTTCCTT AGGCAAGTCA TCCAATTCCA TGTCGAACTT
  181 GAATGGTGCT TCGGCGATGG GCTCGTCACT CGGGTCGTAA TACTGCTCCA GATATGGGTG
  241 GGCCAGAGCC TGTTCTACTT CAATCCTCTT GTGTGGGTTG AATGTCAACA TTTTGTCCAA
  301 TAAGTCCAGA GCTTTGGAGT CAGCATTTGG GAACAGCCTG TTCCATGGCA CCTTATTTTT
  361 GTGTGGAAGA GAAAGCAAAT AGTTCCTAGC TTTTAAATTT ATTATACAAT TCAGGTCTTC
  421 TTGTGATGGG GATCCAAGAA TACCCAAAAT GTGGTTCAGC TGGTCAAGAT AATGCTTCCC
  481 TGGAAAGATG GGCCTGTTAG AAAGCATTTC TGCCAGAATG CAGCCTACAG ACCAAATATC
  541 AATGGACTTG GTGTAGCCCT TGGAATTCAA CATAATTTCT GGAGCCCTGT ACCAACGTGT
  601 GGCCACATAT TCTGTCAGGA ACCCTGTGTG ATCATGGTCT GGATCTGCAA CACGGGCCAG
  661 GCCAAAGTCA CAGATCTTGA GATCACAGGT GGTGTTGAGC AGCAGGTTGG AAGGCTTGAG
  721 GTCACGGTGC AGAACGTTAG CTGAATGGAT ATATTTTAAC CCTCTGAGGA TCTGGTAGAG
  781 AAAATAGCAG ATATGGTCAT TGCTGAGGTG TTGTGTCTTC AAGAGCTTGT AAAGATCTGT
  841 TTCCATGAGG TCCTGTACTA TATATACATC TTTTCATTTT GCTCGATGGT TGGTGCTCGA
  901 ATAATGTCAT TGATTCCAAT GATGTTCTCA TGTCTGAAGC GCAGTAAGAT TTTTTATCTC
  961 CCTCAGGGTT CTCTGGCAGT AGGTCTGGTG CTCAAAAGGG GCTGGATTTT TGTTGATAGC
1021 TACCTCGCAC CTTTGATTGG GACAGTATCC ATTAAGCAGA GCCACCACCG ATTGCCCGT
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB14152_A6Ecd.seq check
>D03441A1_A6Ec_2_SV40pA3'element_Reverse_D04_10.ab1
    1 GAGGTTTGGG GGGGCGGGGC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
  241 TCTGTGCGGT ATTTCACACC GCATAAATTC CGACACCATC GAATGGCGCA AAACCTTTCG
  301 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT
  361 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT
  421 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA
  481 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT
  541 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA
  601 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT
  661 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT
  721 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC
  781 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TTCTCCCATG
  841 AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG CAAATCGCGC
  901 TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGGTCTGC GTCTGGCTGG CTGGCATAAA
  961 ATATCTCACT CGCAAATCAA ATTCAGCCGA TAGCGGAACG GAAGGCGACC TGGAAGTGCA
1021 TGTCCGGATT TTCAACAACC ATGCAAAATG CTTGAAAATG GAGGGCCCA
//
Primer: M13R
Sequence file: RDB14152_A6Ece.seq check
>D03441A1_A6Ec_2_M13R_E04_13.ab1
    1 GACGTGACGT CGTTTTTCAA CGTCGTGACT GGGAAACCCT GGCGTTACCC AACTTAATCG
   61 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG
  121 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC TTTGCCTGGT TTCCGGCACC
  181 AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT GAGGCCGATA CTGTCGTCGT
  241 CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC TACACCAACG TGACCTATCC
  301 CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG ACGGGTTGTT ACTCGCTCAC
  361 ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG CGAATTATTT TTGATGGCGT
  421 TGGAATTAGC TTATCGACTG CACGGTGCAC CAATGCTTCT GGCGTCAGGC AGCCATCGGA
  481 AGCTGTGGTA TGGCTGTGCA GGTCGTAAAT CACTGCATAA TTCGTGTCGC TCAAGGCGCA
  541 CTCCCGTTCT GGATAATGTT TTTTGCGCCG ACATCATAAC GGTTCTGGCA AATATTCTGA
  601 AATGAGCTGT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC
  661 AATTTCACAC AGGAAACAGT ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC
  721 CTTGTGCAAC CCACTCGACT TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG
  781 TATGAGCGCG ATGAAGGTGA TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT
  841 CCCAATCTTC CTTATTATAT TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCCATCAT
  901 ACGTTATATA GCTGACAGCA CAACATGTTT GGGGTGGTTG TCCCAAAAGA GCGTGCAGAG
  961 ATTTCAATGC TTTGAAGGAG CGGGTTTGGA TATTAGAATA CGTGTTTCGA GCACTGCCAT
1021 AATAGTAAGG ACGTGAACTC CTCGAAGATT GATTCTAAGC AAGCCTAACC GAT
//
Primer: EGFP_N_rev
Sequence file: RDB14152_A6Ecf.seq check
>D03441A1_A6Ec_2_EGFP-N_rev_F04_16.ab1
    1 ACTCAAGTTC AGCGTGTCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
   61 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  121 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  181 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  241 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  301 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  361 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  421 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  481 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  541 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  601 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGCCG GACTCAGATC
  661 CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCAT CAACAAGTTT GTACAAAAAA
  721 GCAGGCTTTA TCCCGGCAGC CCATATGGCG GCGGCGGCGG CGGCGGGCGC GGGCCCGGAG
  781 ATGGTCCGCG GGCAGGTGTT CGACGTGGGG CCGCGCTACA CCAACCTCTC GTACATCGGC
  841 GAGGGCGCCT ACGGCATGGT GTGCTCTGCT TATGATAATG TCAACAAAGT TCGAGTAGCT
  901 ATCAAGAAAA TCAGCCCCTT TGAGCACCAG ACCCTACTGC CCAGAGAACC CTGAGGAGAT
  961 AAAAATCTTA CCTGCCGCCT TCAGACATGA GACATCATGG TATCATGACA TATCGAGCAC
1021 AACATCGAGC AATGAAAGAT GTATATATAG TTACAGGATC GC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP]