Resource data sheet
Prev.

EGFR-EGFP

Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP.

Catalog number RDB15996
Resource name EGFR-EGFP
Clone info. Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP at C-terminus.
Expression was confirmed by the depositor with western blot assay and microscopy and image analysis.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human EGFR cDNA
Depositor Sako, Yasushi |
 
Other clones in our bank human EGFR (NCBI Gene 1956) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15996 EGFR-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hiroshima, M., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15996_A8Gkp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFR-N_rev1 RDB15996_A8Gka.seq
>D05656A1_A8GK_2_EGFR-N_rev1_G01_07_ABI08.ab1
    1 GAAGAGGGGA ATTAACCCAG CCCACCTCCT GGATGGTCTT TAAGAAGGAA AGATCATAAT
   61 TCCTCTGCAC ATAGGTAATT TCCAAATTCC CAAGGACCAC CTCACAGTTA TTGAACATCC
  121 TCTGGAGGCT GAGAAAATGA TCTTCAAAAG TGCCCAACTG CGTGAGCTTG TTACTCGTGC
  181 CTTGGCAAAC TTTCTTTTCC TCCAGAGCCC GACTCGCCGG GCAGAGCGCA GCCAGCAGCG
  241 CCAGGAGCGC TGCCCCGGCC GTCCCGGAGG GTCGCATCGC TGCTCCCCGA AGAGCTCGCT
  301 CCGGCTCTCC CGATCAATAC TGGACGGAGT CAGGGGGCCG TGCGCGGTGG TTGTGGCGTT
  361 GGCGGCGAGG CGGGGACTCG GGCGGACGCC GACGAGGTGG CCTGTCGTCC GGTCTGGGCG
  421 GCGGCGGCCG CCGGGGCTAG CTCGGGACTC CGGCCGCCTC GGCCGCGGCG GGCGCTCACA
  481 CCGTGCGGGG GGCGGAGGCT GCTGCGGCCG CGCTGCGCCG GGGGCTGCCC GGACGTCACC
  541 CCCCCCCCCC CCCCCCCTGC AGCAGCTTTT AGAGCAGAAG TAACACTTCC GTACAGGCCT
  601 AGAAGTAAAG GCAACATCCA CTGAGGAGCA GTTCTTTGAT TTGCACCACC ACCGGATCCG
  661 GGACCTGAAA TAAAAGACAA AAAGACTAAA CTTACCAGTT AACTTTCTGG TTTTTCAGTT
  721 CCTCGAGATC TGAGTCCGGT AGCGCTAGCG GATCTGACGG TTCACTAAAC CAGCTCTGCT
  781 TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG GCGGAGTTGT
  841 TACGACATTT TGGAAAGTCC CGTTGATTTT GGGTGCCAAA ACAAAACTCC CATTGACGTC
  901 AATGGGGTGG GAGACTTTGG AAAATCCCCG TGAGTTCAAA ACCGGCTATC CCACGCCCCA
  961 TTTGAATGTA CTGCCCAAAC CCGCATCACC AATGGGTAAA TAGCGAATGA CTATACGTAG
 1021 ATGTTACTGC CCAGTAAGAA AAGTCCCCAT ACGGTCATGT ACTTGGCCAA TATTGCCAGG
 1081 GCGGCCCAAT TACCGTCAAT TGACGGTCAA TTAGGGGCGA TACCTGGCCA TATGAATACA
1141 CCTTTTGA
//
Primer: EGFP-N RDB15996_A8Gkb.seq
>D05656A1_A8GK_2_EGFP-N_F01_06_ABI08.ab1
    1 GACACCCGGG GGGGAAAGCT TCCTCGCCCT TGCTCACCAT GGTGGCGACC GGTGGATCCC
   61 GGGCCCGCGG TACCGTCGAC TGCAGAATTC GAAGCTTTGC TCCAATAAAT TCACTGCTTT
  121 GTGGCGCGAC CCTTAGGTAT TCTGCATTTT CAGCTGTGGA GCCCTTAAAG ATGCCATTTG
  181 GCTTGGCTTC CTTGGGAAAG AAGTCCTGCT GGTAGTCAGG GTTGTCCAGG CTAATTTGGT
  241 GGCTGCCTTT CTGGGCCCAG TGGGCAGGGC TGTCGAATGT GCTGTTGACA CAGGTGGGCT
  301 GGACAGTGTT GAGATACTCG GGGTTGCCCA CTGCAGTGCT GTGGGGGTCC TGGTAGTGTG
  361 GGTCTCTGCT GGGCGCGGGG TTCAGAGGCT GATTGTGATA GACAGGATTC TGCACAGAGC
  421 CAGCGGGCCT TTTGGGAACG GACTGGTTTA TGTATTCAGG CACTGGGAGG AAGGTGTCGT
  481 CTATGCTGTC CTCAGTCAAG GCGCCTGTGG GGTCTGAGCT GTATCGCTGC AAGAAGCTGT
  541 CTTCCTTGAT GGGACAGCTT TGCAGCCCAT TTCTATCAAT GCAAGCCACG GTGGAATTGT
  601 TGCTGGTTGC ACTCAGAGAG CTCAGGAGGG GAGTCCGTGA CGTGGAGGGG CTGCTGAAGA
  661 AGCCCTGCTG TGGGATGAGG TACTCGTCGG CATCCACCAC GTCGTCCATG TCTTCTTCAT
  721 CCATCAGGGC ACGGTAGAAG TTGGAGTCTG TAGGACTTGG CAAATGCATT CTTTCATCCC
  781 CCTGAATGAC AAAGGTAGCG CTGGGGGTCT CGGGCCATTT TGGAGAATTC GATGATCAAC
  841 TCACGGAACT TTGGGCGACC TATCTGCGTC TATCATCCAG CACTTGACCC ATGATCCATG
  901 TAGAACAATC GATGGTACCA TATGGGTGGC TGAGGGGGAG GGCGTTTCTC CCTTTTTCTT
  961 CCAGGATGGA AGGGAGGATT CTCCGCTGGC AAGGATTTCC CGTTCATATT GGCCTTTGGA
 1021 ATCCCAAAGG TCATTCAACT TCCCAAACCG GGTCCACCCC CGTTAGCCTT CCAGAACATC
 1081 CACCTTCTTG GGGTGGGTAT TAGAATTTCC TGGTGGTAAA AATTGGATTC CAATTGCCAT
1141 CCCACTTTGA ATAAGGCACC CTTTTGTGCC TC
//
Primer: pAxEF_R2 RDB15996_A8Gkc.seq
>D05656A1_A8GK_2_pAxEF R2_G01_07_ABI08.ab1
    1 CAAGTTAAGA ATTGGTTTAA TGTTGTGAAA GTTGGTGAGG CTAATTGCTT TATTTGTAAC
   61 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
  121 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  181 TGATTATGAT CTAGAGTCGC GGCCGCTTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT
  241 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT
  301 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC
  361 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG
  421 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT
  481 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC
  541 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG
  601 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG
  661 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG
  721 CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT
  781 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC
  841 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGGATGGGC ACCACCCCGG
  901 TGAACAGCTC CTCGCCCTTG CTCACCATGG GTGGCGACCG GTGGATCCCG GGCCCGCGGG
  961 TACCGTCGAC TGCAGAATTC GAAGCTTTGC TCCAATAATT CACTGCTTTG TGGCGCGACC
 1021 CTTTAGTATT CTGCATTTTT CAGCTGTGAG CCCTTAAGAT GCCCATTTGG CTGCTCTGGG
1081 AAAGGAAGGT TCTGCTGGTT AGTCAAGTGA TCAGGCTTAA TTTGGTGACT GCCATC
//
Primer: SV40pro_F RDB15996_A8Gkd.seq
>D05656A1_A8GK_2_SV40pro_F_H01_08_ABI08.ab1
    1 GCTGGCCGCT AGCCAATTCC AGGAGTAAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT
   61 GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
  661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
  721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
  781 ATTGCTGAAA GAGCTTGGCG GCGGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
  841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG
  901 GACTCTGGGG TTCGAAATGA CCGGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG
  961 AATTCCACCG GCCGCCTTCT ATGAAAAGGT TGGGGCTTCG GAAATCGTTT TTCCGGGGAC
 1021 GCCGGCTGGG ATGAATCCTC CAGCGGCGGG GATCTTCATG GCTGGGAAGT TCCTTCGCCA
 1081 CCCTAAGGGG GAAAGGGCTA ACTGAAATCA CGGAAGAGAC CATTACCGGA TGGTAACCCC
1141 GCCGTCTTAT GTGAACCGGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hiroshima, M., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl