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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

EGFR-EGFP

Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP.

Catalog number RDB15996
Resource name EGFR-EGFP
Clone info. Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP at C-terminus.
Comment Expression was confirmed by the depositor with western blot assay and microscopy and image analysis.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human EGFR cDNA
Depositor|Developer Sako, Yasushi |
Other clones in our bank

External Database
Human EGFR

          Reference sequence
            
           
          Sequence (full) RDB15996zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15996 EGFR-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Hiroshima, M., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15996_A8Gkp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,SV40 intron
          Sequence file: RDB15996_A8Gka.seq check
          >15996_15996_B1EpF_CMV_Forward_D01_04_ABI08.ab1
              1 NNNNNNNNNN NCCNNTTGNC GCNAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGG
            121 AACTGAAAAA CCAGAAAGTT AACTGGTAAG TTTAGTCTTT TTGTCTTTTA TTTCAGGTCC
            181 CGGATCCGGT GGTGGTGCAA ATCAAAGAAC TGCTCCTCAG TGGATGTTGC CTTTACTTCT
            241 AGGCCTGTAC GGAAGTGTTA CTTCTGCTCT AAAAGCTGCT GCAGGGGGGG GGGGGGGGGG
          301 N
          //
          Primer: EGFR-N_rev1
          Region: EGFR 5'
          Sequence file: RDB15996_A8Gkb.seq check
          >D05656A1_A8GK_2_EGFR-N_rev1_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCCT GGNNGGNCTT TAAGAAGGAA AGANCATAAT
             61 TCCTCTGCAC ATAGGTAATT TCCNAATTCC CAAGGACCAC CTCACAGTTA TTGAACATCC
            121 TCTGGAGGCT GAGAAAATGA TCTTCAAAAG TGCCCAACTG CGTGAGCTTG TTACTCGTGC
            181 CTTGGCAAAC TTTCTTTTCC TCCAGAGCCC GACTCGCCGG GCAGAGCGCA GCCAGCAGCG
            241 CCAGGAGCGC TGCCCCGGCC GTCCCGGAGG GTCGCATCGC TGCTCCCCGA AGAGCTCGCT
            301 CCGGCTCTCC CGATCAATAC TGGACGGAGT CAGGGGGCCG TGCGCGGTGG TTGTGGCGTT
            361 GGCGGCGAGG CGGGGACTCG GGCGGACGCC GACGAGGTGG CCTGTCGTCC GGTCTGGGCG
            421 GCGGCGGCCG CCGGGGCTAG CTCGGGACTC CGGCCGCCTC GGCCGCGGCG GGCGCTCACA
            481 CCGTGCGGGG GGCGGAGGCT GCTGCGGCCG CGCTGCGCCG GGGGCTGCCC GGACGTCACC
            541 CCCCCCCCCN NNCCCCCTGC AGCAGCTTTT AGAGCANAAG TAACACTTCC GTACAGGCCT
            601 ANAAGTAAAG GCAACATCCA CTGAGGAGCA GTTCTTTGAT TTGCACCACC ACCGGATCCG
            661 GGACCTGAAA TAAAANACAA AAAGACTAAA CTTACCNGTT AACTTTCNGG TTTTTCNGTT
            721 CCTCNAGATC TGAGTCCGGT AGCGCTAGCG GATCTGACGG TTCNCTAAAC CAGCTCTGCT
            781 TATATAGACC TCCCANNGTA CACGCCTANC GNNNNNTTGC GNCAATGGGG GCGGANNTGT
            841 TACGACNNTT NGGAAAGNNC CGNNGNNTTT NGNTGNCAAA ANNAAACTCC CATTGACGNC
            901 NANGGGGTGG NANNNNTNNG NAANNNCCCN NGAGNNNNAA NCCNNCTNTN CNNNNNCCNA
            961 NTNGNNNGTN NNGNCNNANN CCGNNNNNCC NNNNGNNNAN TNNNGNNNGA NNNNNNNNAN
          1021 NNNNNNNNN
          //
          Primer: Venus-N_R
          Region: EGFP 5',EGFR 3'
          Sequence file: RDB15996_A8Gkc.seq check
          >15996_15996_B1EpF_Venus-N_R_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTNN NGCCGTTTNC GTCGCCGTCC NGCTCGACCA GGATGGGCAC
             61 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC
            121 CCGCGGTACC GTCGACTGCA GAATTCGAAG CTTTGCTCCA ATAAATTCAC TGCTTTGTGG
            181 CGCGACCCTT AGGTATTCTG CATTTTCAGC TGTGGAGCCC TTAAAGATGC CATTTGGCTT
            241 GGCTTCCTTG GGAAAGAAGT CCTGCTGGTA GTCAGGGTTG TCCAGGCTAA TTTGGTGGCT
            301 GCCTTTCTGG GCCCAGTGGG CAGGGCTGTC GAATGTGCTG TTGACACAGG TGGGCTGGAC
            361 AGTGTTGAGA TACTCGGGGT TGCCCACTGC AGTGCTGTGG GGGTCCTGGT AGTGTGGGTC
            421 TCTGCTGGGC GCGGGGTTCA GAGGCTGATT GTGATAGACA GGATTCTGCA CAGAGCCAGC
            481 GGGCCTTTTG GGAACGGACT GGTTTATGTA TTCAGGCACT GGGAGGAAGG TGTCGTCTAT
            541 GCTGTCCTCA GTCAAGGCGC CTGTGGGGTC TGAGCTGTAT CGCTGCAAGA AGCTGTCTTC
            601 CTTGATGGGA CAGCTTTGCA GCCCATTTCT ATCNATGCAA GCCACGGNGG AANTGTTGCT
            661 GNNTGCNCTC NGANANCTCN NNNGGGNNTC CGTGNNGTNN NNGGNCTGNN GNANNNNCNN
          721 GNNNNNNNNN N
          //
          Primer: pAxEF R2
          Region: SV40 pA,EGFP 3'
          Sequence file: RDB15996_A8Gkd.seq check
          >D05656A1_A8GK_2_pAxEF R2_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NNNNGNNNNN NTNNTTGCTN NATTTGTAAC
             61 CANNTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
            121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            181 CTGATTATGA TCTAGAGTCG CGGCCGCTTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA
            241 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT
            301 TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC
            361 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT
            421 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT
            481 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
            541 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
            601 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG
            661 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT
            721 GCACGCCGTA NGTCAGGGTG GTCACGANGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG
            781 TGCAGATGAA CTTCANGGTC AGCTTGCCGT ANGTGGCNTC GCCCTCGCCC TCGCCNGANA
            841 CGCTGAACTN GTGGCCGTTT ACGTCGCCNT CCANCTNGAN CANGNATGGG CNNCACCCNN
            901 GTGAACAGCT NCTCGNCCTT GNNCACCANN GNNGGCNANC NGNGGNNCCC GGGNNNNNNG
          961 NNANNGTCNN NTGNNNAANN NNAANCTTNG NTNNNNNNNN NN
          //
          Primer: SV40pro_F
          Region: NeoR_KanR
          Sequence file: RDB15996_A8Gke.seq check
          >D05656A1_A8GK_2_SV40pro_F_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNGGANGCTT TTTTGGAGGN CTAGGCTTTT
             61 GCNNNNANCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
            121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
            181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
            241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA
            301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
            361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
            421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
            481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
            541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
            601 GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCNACG GCGAGGATCT CGTCGTGACC
            661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGANAATG GCCGCTTTTC TGGATTCATC
            721 GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA TAGCGTTNGC TACCNNNNNN
            781 ATTGCTGAAN NAGCTTGGCG GCNNAATGNG CTGACCGCTT CCNCNNGCTT NACGGTATCG
            841 CCGCTCCCGA NTCGCNNNGN NTCNNCTTCT ATCGCCTTCT TGACNANTTC TTCTGANCNN
            901 NNNTCTGGGG NNNGAAATGN NNNNANNNAG CGACNCCCNA CCTGNCNTCN NNNGATTNCN
            961 ANTNNNNNNN NNCNNNNNCN ATGAAAAGGN TGGGNNTNNN NNANNNNNTT TNNNNGGNNN
          1021 NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hiroshima, M., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756.

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl