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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

EGFR-EGFP (#RDB15996)

Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP.

Clone info. Expression vector of human epidermal growth factor receptor (EGFR) fused with GFP at C-terminus.
Comment Expression was confirmed by the depositor with western blot assay and microscopy and image analysis.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Human EGFR cDNA
Depositor|Developer Sako, Yasushi |
 
Sequence (full) RDB15996zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hiroshima, M. et al., J. Mol. Biol. 430 (9), 1386-1401, 2018)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15996 EGFR-EGFP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The EGFR-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15996).

Reference section:

Hiroshima, M., Pack, C.G., Kaizu, K., Takahashi, K., Ueda, M., Sako, Y., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15996_A8Gkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,SV40 intron
Sequence file: RDB15996_A8Gka.seq check
>15996_15996_B1EpF_CMV_Forward_D01_04_ABI08.ab1
    1 NNNNNNNNNN NCCNNTTGNC GCNAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGAGG
  121 AACTGAAAAA CCAGAAAGTT AACTGGTAAG TTTAGTCTTT TTGTCTTTTA TTTCAGGTCC
  181 CGGATCCGGT GGTGGTGCAA ATCAAAGAAC TGCTCCTCAG TGGATGTTGC CTTTACTTCT
  241 AGGCCTGTAC GGAAGTGTTA CTTCTGCTCT AAAAGCTGCT GCAGGGGGGG GGGGGGGGGG
301 N
//
Primer: EGFR-N_rev1 (Pr0576)
Region: EGFR 5'
Sequence file: RDB15996_A8Gkb.seq check
>D05656A1_A8GK_2_EGFR-N_rev1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCCT GGNNGGNCTT TAAGAAGGAA AGANCATAAT
   61 TCCTCTGCAC ATAGGTAATT TCCNAATTCC CAAGGACCAC CTCACAGTTA TTGAACATCC
  121 TCTGGAGGCT GAGAAAATGA TCTTCAAAAG TGCCCAACTG CGTGAGCTTG TTACTCGTGC
  181 CTTGGCAAAC TTTCTTTTCC TCCAGAGCCC GACTCGCCGG GCAGAGCGCA GCCAGCAGCG
  241 CCAGGAGCGC TGCCCCGGCC GTCCCGGAGG GTCGCATCGC TGCTCCCCGA AGAGCTCGCT
  301 CCGGCTCTCC CGATCAATAC TGGACGGAGT CAGGGGGCCG TGCGCGGTGG TTGTGGCGTT
  361 GGCGGCGAGG CGGGGACTCG GGCGGACGCC GACGAGGTGG CCTGTCGTCC GGTCTGGGCG
  421 GCGGCGGCCG CCGGGGCTAG CTCGGGACTC CGGCCGCCTC GGCCGCGGCG GGCGCTCACA
  481 CCGTGCGGGG GGCGGAGGCT GCTGCGGCCG CGCTGCGCCG GGGGCTGCCC GGACGTCACC
  541 CCCCCCCCCN NNCCCCCTGC AGCAGCTTTT AGAGCANAAG TAACACTTCC GTACAGGCCT
  601 ANAAGTAAAG GCAACATCCA CTGAGGAGCA GTTCTTTGAT TTGCACCACC ACCGGATCCG
  661 GGACCTGAAA TAAAANACAA AAAGACTAAA CTTACCNGTT AACTTTCNGG TTTTTCNGTT
  721 CCTCNAGATC TGAGTCCGGT AGCGCTAGCG GATCTGACGG TTCNCTAAAC CAGCTCTGCT
  781 TATATAGACC TCCCANNGTA CACGCCTANC GNNNNNTTGC GNCAATGGGG GCGGANNTGT
  841 TACGACNNTT NGGAAAGNNC CGNNGNNTTT NGNTGNCAAA ANNAAACTCC CATTGACGNC
  901 NANGGGGTGG NANNNNTNNG NAANNNCCCN NGAGNNNNAA NCCNNCTNTN CNNNNNCCNA
  961 NTNGNNNGTN NNGNCNNANN CCGNNNNNCC NNNNGNNNAN TNNNGNNNGA NNNNNNNNAN
1021 NNNNNNNNN
//
Primer: Venus-N_R (Pr0341)
Region: EGFP 5',EGFR 3'
Sequence file: RDB15996_A8Gkc.seq check
>15996_15996_B1EpF_Venus-N_R_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTNN NGCCGTTTNC GTCGCCGTCC NGCTCGACCA GGATGGGCAC
   61 CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC
  121 CCGCGGTACC GTCGACTGCA GAATTCGAAG CTTTGCTCCA ATAAATTCAC TGCTTTGTGG
  181 CGCGACCCTT AGGTATTCTG CATTTTCAGC TGTGGAGCCC TTAAAGATGC CATTTGGCTT
  241 GGCTTCCTTG GGAAAGAAGT CCTGCTGGTA GTCAGGGTTG TCCAGGCTAA TTTGGTGGCT
  301 GCCTTTCTGG GCCCAGTGGG CAGGGCTGTC GAATGTGCTG TTGACACAGG TGGGCTGGAC
  361 AGTGTTGAGA TACTCGGGGT TGCCCACTGC AGTGCTGTGG GGGTCCTGGT AGTGTGGGTC
  421 TCTGCTGGGC GCGGGGTTCA GAGGCTGATT GTGATAGACA GGATTCTGCA CAGAGCCAGC
  481 GGGCCTTTTG GGAACGGACT GGTTTATGTA TTCAGGCACT GGGAGGAAGG TGTCGTCTAT
  541 GCTGTCCTCA GTCAAGGCGC CTGTGGGGTC TGAGCTGTAT CGCTGCAAGA AGCTGTCTTC
  601 CTTGATGGGA CAGCTTTGCA GCCCATTTCT ATCNATGCAA GCCACGGNGG AANTGTTGCT
  661 GNNTGCNCTC NGANANCTCN NNNGGGNNTC CGTGNNGTNN NNGGNCTGNN GNANNNNCNN
721 GNNNNNNNNN N
//
Primer: pAxEF R2 (Pr0059)
Region: SV40 pA,EGFP 3'
Sequence file: RDB15996_A8Gkd.seq check
>D05656A1_A8GK_2_pAxEF R2_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNNN NNNNGNNNNN NTNNTTGCTN NATTTGTAAC
   61 CANNTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  181 CTGATTATGA TCTAGAGTCG CGGCCGCTTT ACTTGTACAG CTCGTCCATG CCGAGAGTGA
  241 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT
  301 TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC
  361 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT
  421 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT
  481 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
  541 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
  601 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG
  661 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT
  721 GCACGCCGTA NGTCAGGGTG GTCACGANGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG
  781 TGCAGATGAA CTTCANGGTC AGCTTGCCGT ANGTGGCNTC GCCCTCGCCC TCGCCNGANA
  841 CGCTGAACTN GTGGCCGTTT ACGTCGCCNT CCANCTNGAN CANGNATGGG CNNCACCCNN
  901 GTGAACAGCT NCTCGNCCTT GNNCACCANN GNNGGCNANC NGNGGNNCCC GGGNNNNNNG
961 NNANNGTCNN NTGNNNAANN NNAANCTTNG NTNNNNNNNN NN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR_KanR
Sequence file: RDB15996_A8Gke.seq check
>D05656A1_A8GK_2_SV40pro_F_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNGGANGCTT TTTTGGAGGN CTAGGCTTTT
   61 GCNNNNANCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
  241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA
  301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
  361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
  421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
  481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
  541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
  601 GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCNACG GCGAGGATCT CGTCGTGACC
  661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGANAATG GCCGCTTTTC TGGATTCATC
  721 GACTGTGGCC GGCTGGGTGT GGCGGANCGC TATCANGACA TAGCGTTNGC TACCNNNNNN
  781 ATTGCTGAAN NAGCTTGGCG GCNNAATGNG CTGACCGCTT CCNCNNGCTT NACGGTATCG
  841 CCGCTCCCGA NTCGCNNNGN NTCNNCTTCT ATCGCCTTCT TGACNANTTC TTCTGANCNN
  901 NNNTCTGGGG NNNGAAATGN NNNNANNNAG CGACNCCCNA CCTGNCNTCN NNNGATTNCN
  961 ANTNNNNNNN NNCNNNNNCN ATGAAAAGGN TGGGNNTNNN NNANNNNNTT TNNNNGGNNN
1021 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hiroshima, M., Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters. J. Mol. Biol. 430 (9): 1386-1401 (2018). PMID 29505756. [link to RRC of NBRP]