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pDEST/mCherry-PLCD1-PH

Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1).

Catalog number RDB16764
Resource name pDEST/mCherry-PLCD1-PH
Clone info. Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1) fused with mCherry at N-terminus.
Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PLCD1 cDNA
Depositor Sako, Yasushi | Abe, Mitsuhiro |
 
Other clones in our bank human PLCD1 (NCBI Gene 5333) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8):1396−1407, 2012) is requested.
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Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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RDB16764 pDEST/mCherry-PLCD1-PH DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB16764_A8Kmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward RDB16764_A8Kma.seq
>D05982A1_A8Km_1_CMV_Forward_A08_02_ABI24.ab1
    1 TCGTACAACT CCCGCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT ATCAGGTACC ATGGTGAGCA
  181 AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG
  241 AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG
  301 AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG
  361 ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA
  421 TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT
  481 TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA
  541 TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA
  601 AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG
  661 GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA
  721 CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT
  781 TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG
  841 GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGAA GCTTATGGAC TCGGGCCGGG
  901 ACTTCCTGAC CCTGCACGGC CTACAGGATG ATGAGGATCT ACAGGCGCTG CTGAAGGGCA
  961 GCCAGCTCCT GAAAGGTGAA GTCCAGCTCA TGGAGGAGAG AGCGCTTCTA CAAGTTTGCA
 1021 GGAGGACTGC AAGACCAATC TGGCAGAGTC CCGCAAGGTC ATGCGGACCC CGGGAGTCCC
 1081 AGCTGTTCTC ATCGAGGACA TCCAGGAAGT TGCGATTGGG CACGGCACGG AGGGATCTTG
1141 GGAGAAAGGT TCGC
//
Primer: F1ori_F RDB16764_A8Kmb.seq
>D05982A1_A8Km_1_f1ori_F_A11_01_ABI08.ab1
    1 GGGGGCGCCT GGGGCCGCTG GGCAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG
   61 CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT GGGGATACCC CCTAGAGCCC CAGCTGGTTC
  121 TTTCCGCCTC AGAAGCCATA GAGCCCACCG CATCCCCAGC ATGCCTGCTA TTGTCTTCCC
  181 AATCCTCCCC CTTGCTGTCC TGCCCCACCC CACCCCCCAG AATAGAATGA CACCTACTCA
  241 GACAATGCGA TGCAATTTCC TCATTTTATT AGGAAAGGAC AGTGGGAGTG GCACCTTCCA
  301 GGGTCAAGGA AGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG
  361 TCGAGGCTGA TCAGCGGGTT TAAACCTCGA GTCACTTCTG ACGCTGGTCC ATGGAGCCTG
  421 AGTGGTGGAT GATCTTGTGC AGCCCCAGCA CCCAGTGCTG GGCATCAGCT GGCGATGGGG
  481 CGATGAGGTC TAGTGTATTG CGCTGGTCCT TGAAGACAAT GGAGAAGCAG CGGTCCTCGG
  541 GCACATCACG GGCGAACTTC TCCAGACCCT CCGTGCGGTG CCCCATTCGC ACCTCCTGAA
  601 TGTCCTCGAT GGAGAACAGC TGGGACTCCG GGGTCCGCAT GACCTTGCGG GACTCCTGCC
  661 AGATGGTCTT GCAGTCCTCC TGCAACTTGT AGAAGCGCTC TCTCCTCCAT GAGCTGGACT
  721 TCACCTTCAG GAGCTGGCTG CCCTTCAGCA GCGCCTGTAG ATCCTCATCA TCCTGTAGGC
  781 CGTGCAGGGT CAGGAAGTCC CGGCCCGAGT CCATAAGCTT CTTGTACAGC TCGTCCATGC
  841 CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT
  901 TGTGGAGGTG ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT
  961 CTTGGCCCTT GTAGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
 1021 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGAGGCC
 1081 TCCCAGCCCA TGATCTTCTT CTGCATTTAC GGGGTCGTCG AAGGGAGGTT GATTGCCGCG
1141 CAAGCCTTCC ACCTTGCTAG AATTGAACTT CGCCCGTTCT G
//
Primer: SV40pro_F RDB16764_A8Kmc.seq
>D05982A1_A8Km_1_SV40pro_F_D08_11_ABI24.ab1
    1 ACTCGTCCCG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
 1021 TTCGGAATCG TTTTCCCGGG ACGCCGGGCT GGATGATCCT CCAGCGCGGG GATCTTCATG
 1081 CTGCAGGTCT TCGCCCACCC CAACTGGTTA ATGGCAGCCT TAATAATTGT TTACAAAATA
1141 AAGCAATTAG GCCCATTCCA CAGT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463.

2019.03.08

GNP_filter3_RDBDEP_html_190307.pl