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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-PLCD1-PH (#RDB16764)

Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1).

Clone info. Expression vector of the PH domain of human phospholipase C delta 1 (PLC delta 1) fused with mCherry at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence. Insert gene, from HeLa cell cDNA by PCR amplification. Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human PLCD1 cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012) is requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Abe, M et al. Mol. Cell Biol. 32(8)：1396−1407, 2012）の引用を必要とする。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16764 pDEST/mCherry-PLCD1-PH DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-PLCD1-PH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16764).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16764_A8Kmp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward ()
Sequence file: RDB16764_A8Kma.seq check
>D05982A1_A8Km_1_CMV_Forward_A08_02_ABI24.ab1
    1 TCGTACAACT CCCGCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT ATCAGGTACC ATGGTGAGCA
  181 AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG
  241 AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG
  301 AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG
  361 ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA
  421 TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT
  481 TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA
  541 TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA
  601 AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG
  661 GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA
  721 CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT
  781 TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG
  841 GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGAA GCTTATGGAC TCGGGCCGGG
  901 ACTTCCTGAC CCTGCACGGC CTACAGGATG ATGAGGATCT ACAGGCGCTG CTGAAGGGCA
  961 GCCAGCTCCT GAAAGGTGAA GTCCAGCTCA TGGAGGAGAG AGCGCTTCTA CAAGTTTGCA
 1021 GGAGGACTGC AAGACCAATC TGGCAGAGTC CCGCAAGGTC ATGCGGACCC CGGGAGTCCC
 1081 AGCTGTTCTC ATCGAGGACA TCCAGGAAGT TGCGATTGGG CACGGCACGG AGGGATCTTG
1141 GGAGAAAGGT TCGC
//
Primer: F1ori_F ()
Sequence file: RDB16764_A8Kmb.seq check
>D05982A1_A8Km_1_f1ori_F_A11_01_ABI08.ab1
    1 GGGGGCGCCT GGGGCCGCTG GGCAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG
   61 CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT GGGGATACCC CCTAGAGCCC CAGCTGGTTC
  121 TTTCCGCCTC AGAAGCCATA GAGCCCACCG CATCCCCAGC ATGCCTGCTA TTGTCTTCCC
  181 AATCCTCCCC CTTGCTGTCC TGCCCCACCC CACCCCCCAG AATAGAATGA CACCTACTCA
  241 GACAATGCGA TGCAATTTCC TCATTTTATT AGGAAAGGAC AGTGGGAGTG GCACCTTCCA
  301 GGGTCAAGGA AGGCACGGGG GAGGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG
  361 TCGAGGCTGA TCAGCGGGTT TAAACCTCGA GTCACTTCTG ACGCTGGTCC ATGGAGCCTG
  421 AGTGGTGGAT GATCTTGTGC AGCCCCAGCA CCCAGTGCTG GGCATCAGCT GGCGATGGGG
  481 CGATGAGGTC TAGTGTATTG CGCTGGTCCT TGAAGACAAT GGAGAAGCAG CGGTCCTCGG
  541 GCACATCACG GGCGAACTTC TCCAGACCCT CCGTGCGGTG CCCCATTCGC ACCTCCTGAA
  601 TGTCCTCGAT GGAGAACAGC TGGGACTCCG GGGTCCGCAT GACCTTGCGG GACTCCTGCC
  661 AGATGGTCTT GCAGTCCTCC TGCAACTTGT AGAAGCGCTC TCTCCTCCAT GAGCTGGACT
  721 TCACCTTCAG GAGCTGGCTG CCCTTCAGCA GCGCCTGTAG ATCCTCATCA TCCTGTAGGC
  781 CGTGCAGGGT CAGGAAGTCC CGGCCCGAGT CCATAAGCTT CTTGTACAGC TCGTCCATGC
  841 CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT
  901 TGTGGAGGTG ATGTCCAACT TGATGTTGAC GTTGTAGGCG CCGGGCAGCT GCACGGGCTT
  961 CTTGGCCCTT GTAGTGGTCT TGACCTCAGC GTCGTAGTGG CCGCCGTCCT TCAGCTTCAG
 1021 CCTCTGCTTG ATCTCGCCCT TCAGGGCGCC GTCCTCGGGG TACATCCGCT CGGAGAGGCC
 1081 TCCCAGCCCA TGATCTTCTT CTGCATTTAC GGGGTCGTCG AAGGGAGGTT GATTGCCGCG
1141 CAAGCCTTCC ACCTTGCTAG AATTGAACTT CGCCCGTTCT G
//
Primer: SV40pro_F ()
Sequence file: RDB16764_A8Kmc.seq check
>D05982A1_A8Km_1_SV40pro_F_D08_11_ABI24.ab1
    1 ACTCGTCCCG GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
 1021 TTCGGAATCG TTTTCCCGGG ACGCCGGGCT GGATGATCCT CCAGCGCGGG GATCTTCATG
 1081 CTGCAGGTCT TCGCCCACCC CAACTGGTTA ATGGCAGCCT TAATAATTGT TTACAAAATA
1141 AAGCAATTAG GCCCATTCCA CAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]