Sequencing and PCR primers for primer information.
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
| Catalog number | RDB13496 |
|---|---|
| Resource name | pET28/EqtII-EGFP-His6 |
| Clone info. | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. Optimized for expression in E. coli |
| Vector backbone | pET-28b (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Kan^r |
| Gene/insert name | Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA |
| Depositor | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13496 | pET28/EqtII-EGFP-His6 | DNA solution |
Electronic file
Featured content
| Featured content | Sphingolipid signaling pathway (English text) |
|---|---|
| Featured content | Organelle marker (English text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13496_A6Kap1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pET-check-F | RDB13496_A6Kaa.seq |
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>13496_13496_A6Ka_2_pET-check-F_B06_06_ABI24.ab1 1 CGATATCTCA CAGTCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC 61 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA 121 GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG 181 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT 241 CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCGGTAC CATGAGCGCG 301 GATGTGGCTG GTGCAGTCAT AGATGGCGCC TCTCTGAGTT TCGACATCCT CAAGACCGTG 361 TTGGAAGCTC TGGGGAATGT CAAACGGAAG ATTGCCGTAG GAGTGGACAA CGAAAGCGGT 421 AAGACATGGA CTGCCCTGAA TACGTACTTC AGGTCTGGAA CCAGCGACAT TGTTCTGCCC 481 CACAAAGTCC CACATGGGAA AGCACTCCTG TACAATGGGC AGAAAGATCG AGGACCTGTT 541 GCGACTGGTG CAGTTGGGGT ATTGGCCTAT CTCATGTCAG ATGGCAACAC CCTTGCTGTG 601 CTGTTTTCCG TCCCCTATGA CTACAACTGG TATTCCAACT GGTGGAATGT GCGCATCTAC 661 AAGGGCAAAC GTAGAGCCGA TCAACGCATG TATGAGGAGC TTTACTACAA CCTGTCCCCG 721 TTTAGGGGCG ACAATGGATG GCACACACGG AATCTCGGGT ATGGCCTGAA GAGCAGAGGC 781 TTCATGAACA GCAGTGGCCA TGCCATCCTG GAGATTCACG TGTCCAAGGC TGGTGGAGGG 841 TCAGGCGGCG GATCTGAATT CGGAGGAGGA GGGAGGAGGA TCCATGGTGA GCAAGGGCCG 901 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTTCGAGC TGGACGGCGA CGTAAACGGG 961 CCAACAAGTT CAGCGTGTCC CGGCGAGGGC GAGGGCGATG CCACCTATGG CAAGCTGACC 1021 CTGAAGGTCA TCTGCACCAC CGGCAAGCCT GCCGTGCCTG ACCACCCTCG TGACCACCCT 1081 GACCTACCGG CGTGCAGTGC CTTCAAGCCG CTTACCCGAC ACATGGAGCA GCCACGGACC 1141 TTCTTCCAGG TCCGCCCCAT GCTCCCGAAG GCCT // |
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| Primer: f1ori_R3 | RDB13496_A6Kab.seq |
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>13496_13496_A6Ka_2_f1ori_R3_D06_12_ABI24.ab1 1 GCATTGCAAT CGGATTAGTT CCTCCTTTCA GCAAAAACCC CTCAAGACCC GTTTAGAGGC 61 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG 121 GGCTTTGTTA GCAGCCGGAT CTCAGTGGTG GTGGTGGTGG TGAAGCTTCT TGTACAGCTC 181 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG 241 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG 301 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT 361 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC 421 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT 481 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC 541 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC 601 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG 661 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC 721 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCATAGG TGGCATCGCC 781 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG 841 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGATC CTCCTCCTCC 901 TCCTCCGAAT TCAGATCCGC CGCCTGACCC TCCACCAGCC TTGGACACGT GAATCTCCAG 961 GATGGCATGG CCCACTGCTG TTCATGAAAG CCCTCTGCTC TTCAGCCATA CCCGAGATTT 1021 CCGTGTGTGC CATCCCATTG TCGCCCCTAA ACGGGGACAG ATGTAGTAAG CTCCCTCATA 1081 ACATGCGATG ATCGGGCTCT ACGGTTTTGC CCCTTGTAGA TGCGCACATC ATCAAGTGAA 1141 TTACCAGTGG TAAGTCATAA GGGGACCGGA AAAACAG // |
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| Primer: pGL3_RV4 | RDB13496_A6Kac.seq |
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>13496_13496_A6Ka_2_pGL3-RV4_F06_18_ABI24.ab1 1 CCAACACTGA TGGGTTGAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT 61 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 121 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 181 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT 241 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT 301 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA 361 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA 421 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT 481 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT 541 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG 601 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA 661 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG 721 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT 781 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA 841 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC 901 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGCGCGT GCAGGGCCAG 961 ACTGGATGTG GCAACGCCAT CAGCAACGAC TGTTGCCCGC CAGTTGTGTG CCACGCGGTT 1021 GGGATGTATT CAGCTCCGGC CATCGCCGCT TCACTTTTTC CCGCGTTTCG CAGAAACGTG 1081 CTGCTGATCA CACGCGGGAC GGTCTGATAG AGACACCGGC TTACTCTGGC GACATCGTAA 1141 TAACGGTTAC CTGGTC // |
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Please visit Sequencing and PCR primers for primer information.
References
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl