Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/EqtII-EGFP-His6

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.

Sequence (full)	RDB13496zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15961_A8E2p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F (Pr0191) Region: T7 pro,lacO,RBS,insert 5'(EqtII 5') Sequence file: RDB15961_A8E2j.seq
>RDB13496_15961_3_A8E2_pET-check-F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGN NNGCCNCCNN ACCCNCGCCG AANCNAGCGC    61 TCNTGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC   121 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG   181 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA   241 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCGGT ACCATGAGCG   301 CGGATGTGGC TGGTGCAGTC ATAGATGGCG CCTCTCTGAG TTTCGACATC CTCAAGACCG   361 TGTTGGAAGC TCTGGGGAAT GTCAAACGGA AGATTGCCGT AGGAGTGGAC AACGAAAGCG   421 GTAAGACATG GACTGCCCTG AATACGTACT TCAGGTCTGG AACCAGCGAC ATTGTTCTGC   481 CCCACAAAGT CCCACATGGG AAAGCACTCC TGTACAATGG GCAGAAAGAT CGAGGACCTG   541 TTGCGACTGG TGCAGTTGGG GTATTGGCCT ATCTCATGTC AGATGGCAAC ACCCTTGCTG   601 TGCTGTTTTC CGTCCCCTAT GACTACAACT GGTATTCCAA CTGGTGGAAT GTGCGCATCT   661 ACAAGGGCAA ACGTAGAGCC GATCAACGCA TGTATGAGGA GCTTTACTAC AACCTGTCCC   721 CGTTTANGGG CGACAATGGA TGGCACACAC GGAATCTCGG GTATGGCCTG NAGANCAGAN   781 GCTTCATGAN CAGCAGTGNC CNTGCCATCC TGGANATTCA CGTGTCCNAN GCTGGNGGAN   841 GGTCANGCGG CGGATCTGAA NTCNGANGAN GANGANGANG NNCCATGGTG ANCNANGGNN   901 NANGANCTGN TCACCGGGGN GGNGCCCNTC CNNNNCNANC TNNNNNGNNN NGTNNNNNNN 961 NNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter,6xHis,insert 3'(EGFP 3') Sequence file: RDB15961_A8E2k.seq
>13496_15961_A8E2_3_f1ori_R3_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CTTTNNNNNN NNNNCCCCTC AAGANCCGTT    61 NAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT   121 CCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGA AGCTTCTTGT   181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT   241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT   301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT   361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT   421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCANC TTGTGCCCCA   481 NGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT   541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTANTTGCC GTCGTCCTTG AAGAAGATGG   601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT   661 GGTCGGGGTA NCGGCTGAAG CACTGCACGC CGTANGTCAG GGTGGTCACG ANGGTGGGCC   721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGNCAGCTTG CNNTANGTGG   781 CATCGCCCTC GCCCTCGCCN GANACNNNTG AANCTTGTGG CCGTTNACGT CNCCGTCCNN   841 CTCGACNANN NNGGGCNNCA CCCCCGGGTG AANCAGCTCC TCGCCCTTGC TCACCATGGG   901 ATCCNNNNNN NNNCNTNNTN NNNNAATTNN NATCCGCCNN NNNTNNANCC NNNNNCNNNN   961 CCNTNGGNAN NNNNNNNANT CTNNNNGGAN NGGCANNGNN NNNNNNCTNN TNNANNNANN 1021 NCNNTNNNTN NNNNNNNNN //
Primer: Tet-r-M_R (Pr0204) Region: lacI Sequence file: RDB15961_A8E2l.seq
>13496_15961_A8E2_3_Tet-r-M_R_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCTGNNT GGGTNNNNNG NTCTCNAGGG    61 CATCGGTCGA GATCCCGGTG NNNNNNNNNN NNNNNNNNTT ACATTAATTG CGTTGCGCTC   121 ACTGCCCNNN NTCCNGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG   181 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA   241 CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA   301 CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC   361 ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT ATCCGCACCA ACGCGCAGCC   421 CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG   481 CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC   541 TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC   601 AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT   661 GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT TCATGGGAGA   721 AAATAATACT GTTGATGGGT GTCTGGTCAG AGNCATCAAG AAATAACGCC GGAACATTAG   781 TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CNGATAGTNA ATGATCAGCC   841 CACTGNCGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCN ANGCCGCTNC   901 NNTCTNCCNT CGACACCACN ACGCNNGNNN CCAGTTGNTN GGNNCNNNNN TTNATCGCCN   961 CGNNNNNTNG CGNNNNNNCN NGCNNNNNCN NNNNGGATNN GNNNNNNNCN NANNNNNNNN 1021 NN //
Primer: EGFP-M_R (Pr0743) Region: insert mid(EGFP 5') Sequence file: RDB15961_A8E2m.seq
>13496_15961_B0C4F_EGFP-M_R_H07_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG TGNNGGNNCT TGAGTTCNCC TTGNTGCCGT TCTTCTGCTT    61 GTCGGCCATG NTATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT   121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC   181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG   241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC   301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG   361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCAT AGGTGGCATC   421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC   481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG ATCCTCCTCC   541 TCCTCCTCCG AATTCANATC CGCCGCCTGA NCCTCCACCA GCCTTGGANA CGTGAATCTC   601 CNGGANGGCA TGGCCACTGC TGNNCATGAA GCCTCNGNTC NTCNNNNNTA NCCGANANTN   661 CGNGTGTGNN NTNNNTTGNC GCCCCTAAAC GGGGNNNGNN NGNANTAAAG CTNNNNNNAN   721 NTGCGTNGAT NNNNTCNACN TTNGNCNNNN NNNANGNNCA CANTNNNNNA NNNNNNNNNN //
Primer: YFP_R (Pr0127) Region: insert 5'(EGFP 5',EqtII 3'),RBS,lacO Sequence file: RDB15961_A8E2n.seq
>13496_15961_B2J3F_YFP_R_B02_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGA NTTGTGGCCG TTTNCGTCGC CGTCCNGCTC    61 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGATCCTCC   121 TCCTCCTCCT CCGAATTCAG ATCCGCCGCC TGACCCTCCA CCAGCCTTGG ACACGTGAAT   181 CTCCAGGATG GCATGGCCAC TGCTGTTCAT GAAGCCTCTG CTCTTCAGGC CATACCCGAG   241 ATTCCGTGTG TGCCATCCAT TGTCGCCCCT AAACGGGGAC AGGTTGTAGT AAAGCTCCTC   301 ATACATGCGT TGATCGGCTC TACGTTTGCC CTTGTAGATG CGCACATTCC ACCAGTTGGA   361 ATACCAGTTG TAGTCATAGG GGACGGAAAA CAGCACAGCA AGGGTGTTGC CATCTGACAT   421 GAGATAGGCC AATACCCCAA CTGCACCAGT CGCAACAGGT CCTCGATCTT TCTGCCCATT   481 GTACAGGAGT GCTTTCCCAT GTGGGACTTT GTGGGGCAGA ACAATGTCGC TGGTTCCAGA   541 CCTGAAGTAC GTATTCAGGG CAGTCCATGT CTTACCGCTT TCGTTGTCCA CTCCTACGGC   601 AATCTTCCGT TTGACATTCC CCAGAGCTTC CAACACGGTC TTGAGGATGT CGAAACTCAG   661 AGAGGCGCCA TCTATGACTG CACCAGCCAC ATCCGCGCTC ATGGTACCGG TATATCTCCT   721 TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAANTGTT ATCCGCTCAC AATTCCCCTA   781 TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCTCGATC CTCTACGCCN GANGCATCGT   841 GGCCGGCATC ACCGGCGCCN CANGTGCNGT TGCTGGCGCC NATATCGNCN ANNTCACCGA   901 NNGGGAAGAT CGGGNNCGCC ACTNNGGGCT CNNGANCGCT NGNTTCGGCN NNGGTANGGT 961 GNNNNNNCCC NNNNNNCNGG GGGNNTGNTN GGGNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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