Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/EqtII-EGFP-His6

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 [Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15961_A8E2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F Region: T7 pro, lacO, RBS, insert, EGFP 5' Sequence file: RDB15961_A8E2a.seq
>RDB13496_15961_3_A8E2_pET-check-F_C01_03_ABI08.ab1     1 GGTAAAGGGG TCCACAGTCC CGGCACGGGG CTGCCACCAT ACCCACGCCG AAACAAGCGC    61 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC   121 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG   181 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA   241 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCGGT ACCATGAGCG   301 CGGATGTGGC TGGTGCAGTC ATAGATGGCG CCTCTCTGAG TTTCGACATC CTCAAGACCG   361 TGTTGGAAGC TCTGGGGAAT GTCAAACGGA AGATTGCCGT AGGAGTGGAC AACGAAAGCG   421 GTAAGACATG GACTGCCCTG AATACGTACT TCAGGTCTGG AACCAGCGAC ATTGTTCTGC   481 CCCACAAAGT CCCACATGGG AAAGCACTCC TGTACAATGG GCAGAAAGAT CGAGGACCTG   541 TTGCGACTGG TGCAGTTGGG GTATTGGCCT ATCTCATGTC AGATGGCAAC ACCCTTGCTG   601 TGCTGTTTTC CGTCCCCTAT GACTACAACT GGTATTCCAA CTGGTGGAAT GTGCGCATCT   661 ACAAGGGCAA ACGTAGAGCC GATCAACGCA TGTATGAGGA GCTTTACTAC AACCTGTCCC   721 CGTTTAGGGG CGACAATGGA TGGCACACAC GGAATCTCGG GTATGGCCTG AAGAGCAGAG   781 GCTTCATGAA CAGCAGTGGC CATGCCATCC TGGAGATTCA CGTGTCCAAG GCTGGTGGAG   841 GGTCAGGCGG CGGATCTGAA TTCGGAGGAG GAGGAGGAGG ATCCATGGTG AGCAAAGGGC   901 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGATCGAGC TGGACGGCGA CGTAACGGGC   961 CACAAGTTCA GCGTGTCCCG GCGAGGGCGA GGGCGATGCC ACCTATGGCA AGCTGACCCT  1021 GAGGTCCATC CTGCACACCG GGCAAGCCTG CCCGTTGCCC CTGACCCATC GTCGTGACCA 1081 CCCTTGAACA TACGACGTTG CCAGGTGGCT TCCAGTC //
Primer: f1ori_R3 Region: T7 ter, 6xHis, EGFP 3' Sequence file: RDB15961_A8E2b.seq
>13496_15961_A8E2_3_f1ori_R3_B01_02_ABI08.ab1     1 AGATTTAGCC ATCCGGGGAA TTAGTTCCTC CTTTCAGCAA AAAACCCCTC AAGACCCGTT    61 TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT   121 CCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGA AGCTTCTTGT   181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT   241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT   301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT   361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT   421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA   481 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT   541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG   601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT   661 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC   721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCATAGGTGG   781 CATCGCCCTC GCCCTCGCCG GACACGGCTG AAACTTGTGG CCGTTTACGT CGCCGTCCAG   841 CTCGACCAGG ATGGGCACCA CCCCCGGGTG AAACAGCTCC TCGCCCTTGC TCACCATGGG   901 ATCCTCCTTC CTCCCTCCTC CCGAAATTCA GATCCGCCCG CCCTGGACCC TCCACCCAGC   961 CCTTTGGGAC ACGGTGAAAT CTCCAGGGAT GGGCATGGGC CACTGGCTGG TTCATGGAAG  1021 CCTCTGCCTC TTCAGGCCAG TAACCCCGAG AATTTCCGGT GGTGGTGCCC ATCCCATTGT  1081 CCGGCCCCCA TAATGGGGGG ACAAGGGTTG GTAGGTAAAG CTTCTAATAC ATGCCGGTGG 1141 GATCCGGCCC TCTATCGGGT TATTGGTCAC CTA //
Primer: Tet-r-M_R Region: lacI Sequence file: RDB15961_A8E2c.seq
>13496_15961_A8E2_3_Tet-r-M_R_H02_23_ABI24.ab1     1 GTGCCTAAAA AGTGGGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT    61 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT   121 TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG   181 CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC   241 AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA   301 TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG   361 CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC   421 TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT   481 TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA   541 CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG   601 ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG   661 GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA   721 GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG   781 AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC   841 ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC   901 GCGTGCAGGG CCAGACTGGA TGTGGCAACG CCCATCAGCA ACGACTGTTT GCCCGCCAGT   961 GGTGTGCCAC GCGATTGGGA TGTAATCAGC CTCGCCATCG CGCATCACTT TTTTCCCGCG 1021 TTTCGCAGAA CGTGTCTTGC CTGGTTC //
Primer: EGFP-M_R Region: EGFP 5', insert 3' Sequence file: RDB15961_A8E2d.seq
>13496_15961_B0C4F_EGFP-M_R_H07_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG TGNNGGNNCT TGAGTTCNCC TTGNTGCCGT TCTTCTGCTT    61 GTCGGCCATG NTATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT   121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC   181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG   241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC   301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG   361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCAT AGGTGGCATC   421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC   481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG ATCCTCCTCC   541 TCCTCCTCCG AATTCANATC CGCCGCCTGA NCCTCCACCA GCCTTGGANA CGTGAATCTC   601 CNGGANGGCA TGGCCACTGC TGNNCATGAA GCCTCNGNTC NTCNNNNNTA NCCGANANTN   661 CGNGTGTGNN NTNNNTTGNC GCCCCTAAAC GGGGNNNGNN NGNANTAAAG CTNNNNNNAN   721 NTGCGTNGAT NNNNTCNACN TTNGNCNNNN NNNANGNNCA CANTNNNNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl