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pET28/EqtII-EGFP-His6

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.

Catalog number RDB13496
Resource name pET28/EqtII-EGFP-His6
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
Optimized for expression in E. coli
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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Schedule A (GFP-A) [Link]
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RDB13496 pET28/EqtII-EGFP-His6 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15961_A8E2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F
Region: T7 pro, lacO, RBS, insert, EGFP 5'
Sequence file: RDB15961_A8E2a.seq check
>RDB13496_15961_3_A8E2_pET-check-F_C01_03_ABI08.ab1
    1 GGTAAAGGGG TCCACAGTCC CGGCACGGGG CTGCCACCAT ACCCACGCCG AAACAAGCGC
   61 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
  121 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
  181 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
  241 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCGGT ACCATGAGCG
  301 CGGATGTGGC TGGTGCAGTC ATAGATGGCG CCTCTCTGAG TTTCGACATC CTCAAGACCG
  361 TGTTGGAAGC TCTGGGGAAT GTCAAACGGA AGATTGCCGT AGGAGTGGAC AACGAAAGCG
  421 GTAAGACATG GACTGCCCTG AATACGTACT TCAGGTCTGG AACCAGCGAC ATTGTTCTGC
  481 CCCACAAAGT CCCACATGGG AAAGCACTCC TGTACAATGG GCAGAAAGAT CGAGGACCTG
  541 TTGCGACTGG TGCAGTTGGG GTATTGGCCT ATCTCATGTC AGATGGCAAC ACCCTTGCTG
  601 TGCTGTTTTC CGTCCCCTAT GACTACAACT GGTATTCCAA CTGGTGGAAT GTGCGCATCT
  661 ACAAGGGCAA ACGTAGAGCC GATCAACGCA TGTATGAGGA GCTTTACTAC AACCTGTCCC
  721 CGTTTAGGGG CGACAATGGA TGGCACACAC GGAATCTCGG GTATGGCCTG AAGAGCAGAG
  781 GCTTCATGAA CAGCAGTGGC CATGCCATCC TGGAGATTCA CGTGTCCAAG GCTGGTGGAG
  841 GGTCAGGCGG CGGATCTGAA TTCGGAGGAG GAGGAGGAGG ATCCATGGTG AGCAAAGGGC
  901 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGATCGAGC TGGACGGCGA CGTAACGGGC
  961 CACAAGTTCA GCGTGTCCCG GCGAGGGCGA GGGCGATGCC ACCTATGGCA AGCTGACCCT
 1021 GAGGTCCATC CTGCACACCG GGCAAGCCTG CCCGTTGCCC CTGACCCATC GTCGTGACCA
1081 CCCTTGAACA TACGACGTTG CCAGGTGGCT TCCAGTC
//
Primer: f1ori_R3
Region: T7 ter, 6xHis, EGFP 3'
Sequence file: RDB15961_A8E2b.seq check
>13496_15961_A8E2_3_f1ori_R3_B01_02_ABI08.ab1
    1 AGATTTAGCC ATCCGGGGAA TTAGTTCCTC CTTTCAGCAA AAAACCCCTC AAGACCCGTT
   61 TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT
  121 CCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGA AGCTTCTTGT
  181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
  241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
  301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
  361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT
  421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA
  481 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
  541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
  601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
  661 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC
  721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCATAGGTGG
  781 CATCGCCCTC GCCCTCGCCG GACACGGCTG AAACTTGTGG CCGTTTACGT CGCCGTCCAG
  841 CTCGACCAGG ATGGGCACCA CCCCCGGGTG AAACAGCTCC TCGCCCTTGC TCACCATGGG
  901 ATCCTCCTTC CTCCCTCCTC CCGAAATTCA GATCCGCCCG CCCTGGACCC TCCACCCAGC
  961 CCTTTGGGAC ACGGTGAAAT CTCCAGGGAT GGGCATGGGC CACTGGCTGG TTCATGGAAG
 1021 CCTCTGCCTC TTCAGGCCAG TAACCCCGAG AATTTCCGGT GGTGGTGCCC ATCCCATTGT
 1081 CCGGCCCCCA TAATGGGGGG ACAAGGGTTG GTAGGTAAAG CTTCTAATAC ATGCCGGTGG
1141 GATCCGGCCC TCTATCGGGT TATTGGTCAC CTA
//
Primer: Tet-r-M_R
Region: lacI
Sequence file: RDB15961_A8E2c.seq check
>13496_15961_A8E2_3_Tet-r-M_R_H02_23_ABI24.ab1
    1 GTGCCTAAAA AGTGGGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT
   61 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT
  121 TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG
  181 CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC
  241 AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA
  301 TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG
  361 CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC
  421 TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT
  481 TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA
  541 CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG
  601 ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG
  661 GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA
  721 GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG
  781 AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC
  841 ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC
  901 GCGTGCAGGG CCAGACTGGA TGTGGCAACG CCCATCAGCA ACGACTGTTT GCCCGCCAGT
  961 GGTGTGCCAC GCGATTGGGA TGTAATCAGC CTCGCCATCG CGCATCACTT TTTTCCCGCG
1021 TTTCGCAGAA CGTGTCTTGC CTGGTTC
//
Primer: EGFP-M_R
Region: EGFP 5', insert 3'
Sequence file: RDB15961_A8E2d.seq check
>13496_15961_B0C4F_EGFP-M_R_H07_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG TGNNGGNNCT TGAGTTCNCC TTGNTGCCGT TCTTCTGCTT
   61 GTCGGCCATG NTATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT
  121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC
  181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG
  241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC
  301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG
  361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCAT AGGTGGCATC
  421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
  481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG ATCCTCCTCC
  541 TCCTCCTCCG AATTCANATC CGCCGCCTGA NCCTCCACCA GCCTTGGANA CGTGAATCTC
  601 CNGGANGGCA TGGCCACTGC TGNNCATGAA GCCTCNGNTC NTCNNNNNTA NCCGANANTN
  661 CGNGTGTGNN NTNNNTTGNC GCCCCTAAAC GGGGNNNGNN NGNANTAAAG CTNNNNNNAN
  721 NTGCGTNGAT NNNNTCNACN TTNGNCNNNN NNNANGNNCA CANTNNNNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

2020.03.19

GNP_filter3_RDBDEP_html_200316.pl