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pET28/EqtII-EGFP-His6 (#RDB13496)

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Makino, A., FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
Comment Optimized for expression in E. coli
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Growth conditions LB + Kan, 37 oC
Gene/insert name Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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必要書類
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。

Catalog # Resource name Shipping form Fee
RDB13496 pET28/EqtII-EGFP-His6 DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/EqtII-EGFP-His6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13496).

Reference section:

Makino, A., Abe, M., Murate, M., Inaba, T., Yilmaz, N., Hullin-Matsuda, F., Kishimoto, T., Schieber, N.L., Taguchi, T., Arai, H., Anderluh, G., Parton, R.G., Kobayashi, T., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [PubMed] [Article] [RRC of NBRP]