Resource data sheet
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pET28/EqtII-EGFP-His6

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.

Catalog number RDB13496
Resource name pET28/EqtII-EGFP-His6
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
Optimized for expression in E. coli
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13496 pET28/EqtII-EGFP-His6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

References

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13496_A6Kap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F RDB13496_A6Kaa.seq
>13496_13496_A6Ka_2_pET-check-F_B06_06_ABI24.ab1
    1 CGATATCTCA CAGTCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC
   61 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA
  121 GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG
  181 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT
  241 CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCGGTAC CATGAGCGCG
  301 GATGTGGCTG GTGCAGTCAT AGATGGCGCC TCTCTGAGTT TCGACATCCT CAAGACCGTG
  361 TTGGAAGCTC TGGGGAATGT CAAACGGAAG ATTGCCGTAG GAGTGGACAA CGAAAGCGGT
  421 AAGACATGGA CTGCCCTGAA TACGTACTTC AGGTCTGGAA CCAGCGACAT TGTTCTGCCC
  481 CACAAAGTCC CACATGGGAA AGCACTCCTG TACAATGGGC AGAAAGATCG AGGACCTGTT
  541 GCGACTGGTG CAGTTGGGGT ATTGGCCTAT CTCATGTCAG ATGGCAACAC CCTTGCTGTG
  601 CTGTTTTCCG TCCCCTATGA CTACAACTGG TATTCCAACT GGTGGAATGT GCGCATCTAC
  661 AAGGGCAAAC GTAGAGCCGA TCAACGCATG TATGAGGAGC TTTACTACAA CCTGTCCCCG
  721 TTTAGGGGCG ACAATGGATG GCACACACGG AATCTCGGGT ATGGCCTGAA GAGCAGAGGC
  781 TTCATGAACA GCAGTGGCCA TGCCATCCTG GAGATTCACG TGTCCAAGGC TGGTGGAGGG
  841 TCAGGCGGCG GATCTGAATT CGGAGGAGGA GGGAGGAGGA TCCATGGTGA GCAAGGGCCG
  901 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTTCGAGC TGGACGGCGA CGTAAACGGG
  961 CCAACAAGTT CAGCGTGTCC CGGCGAGGGC GAGGGCGATG CCACCTATGG CAAGCTGACC
 1021 CTGAAGGTCA TCTGCACCAC CGGCAAGCCT GCCGTGCCTG ACCACCCTCG TGACCACCCT
 1081 GACCTACCGG CGTGCAGTGC CTTCAAGCCG CTTACCCGAC ACATGGAGCA GCCACGGACC
1141 TTCTTCCAGG TCCGCCCCAT GCTCCCGAAG GCCT
//
Primer: f1ori_R3 RDB13496_A6Kab.seq
>13496_13496_A6Ka_2_f1ori_R3_D06_12_ABI24.ab1
    1 GCATTGCAAT CGGATTAGTT CCTCCTTTCA GCAAAAACCC CTCAAGACCC GTTTAGAGGC
   61 CCCAAGGGGT TATGCTAGTT ATTGCTCAGC GGTGGCAGCA GCCAACTCAG CTTCCTTTCG
  121 GGCTTTGTTA GCAGCCGGAT CTCAGTGGTG GTGGTGGTGG TGAAGCTTCT TGTACAGCTC
  181 GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG
  241 CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG
  301 CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT
  361 GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC
  421 GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT
  481 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC
  541 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC
  601 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG
  661 GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC
  721 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCATAGG TGGCATCGCC
  781 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG
  841 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGGATC CTCCTCCTCC
  901 TCCTCCGAAT TCAGATCCGC CGCCTGACCC TCCACCAGCC TTGGACACGT GAATCTCCAG
  961 GATGGCATGG CCCACTGCTG TTCATGAAAG CCCTCTGCTC TTCAGCCATA CCCGAGATTT
 1021 CCGTGTGTGC CATCCCATTG TCGCCCCTAA ACGGGGACAG ATGTAGTAAG CTCCCTCATA
 1081 ACATGCGATG ATCGGGCTCT ACGGTTTTGC CCCTTGTAGA TGCGCACATC ATCAAGTGAA
1141 TTACCAGTGG TAAGTCATAA GGGGACCGGA AAAACAG
//
Primer: pGL3_RV4 RDB13496_A6Kac.seq
>13496_13496_A6Ka_2_pGL3-RV4_F06_18_ABI24.ab1
    1 CCAACACTGA TGGGTTGAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT
   61 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
  121 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
  181 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
  241 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
  301 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
  361 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
  421 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
  481 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
  541 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
  601 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
  661 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
  721 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
  781 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA
  841 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC
  901 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGCGCGT GCAGGGCCAG
  961 ACTGGATGTG GCAACGCCAT CAGCAACGAC TGTTGCCCGC CAGTTGTGTG CCACGCGGTT
 1021 GGGATGTATT CAGCTCCGGC CATCGCCGCT TCACTTTTTC CCGCGTTTCG CAGAAACGTG
 1081 CTGCTGATCA CACGCGGGAC GGTCTGATAG AGACACCGGC TTACTCTGGC GACATCGTAA
1141 TAACGGTTAC CTGGTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

2018.12.10

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