Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
Catalog number | RDB13496 |
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Resource name | pET28/EqtII-EGFP-His6 |
Clone info. | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. Optimized for expression in E. coli |
Vector backbone | pET-28b (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Kan^r |
Gene/insert name | Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015) |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA) [Word ![]() Schedule A (GFP-A) [Link] |
Remarks | Please use Schedule A (GFP-A) additionally. |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13496 | pET28/EqtII-EGFP-His6 | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Sphingolipid signaling pathway (English text) |
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Featured content | Visualization of Organelles (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15961_A8E2p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pET-check-F Region: T7 pro, lacO, RBS, insert, EGFP 5' Sequence file: RDB15961_A8E2a.seq ![]() |
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>RDB13496_15961_3_A8E2_pET-check-F_C01_03_ABI08.ab1 1 GGTAAAGGGG TCCACAGTCC CGGCACGGGG CTGCCACCAT ACCCACGCCG AAACAAGCGC 61 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC 121 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG 181 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA 241 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCGGT ACCATGAGCG 301 CGGATGTGGC TGGTGCAGTC ATAGATGGCG CCTCTCTGAG TTTCGACATC CTCAAGACCG 361 TGTTGGAAGC TCTGGGGAAT GTCAAACGGA AGATTGCCGT AGGAGTGGAC AACGAAAGCG 421 GTAAGACATG GACTGCCCTG AATACGTACT TCAGGTCTGG AACCAGCGAC ATTGTTCTGC 481 CCCACAAAGT CCCACATGGG AAAGCACTCC TGTACAATGG GCAGAAAGAT CGAGGACCTG 541 TTGCGACTGG TGCAGTTGGG GTATTGGCCT ATCTCATGTC AGATGGCAAC ACCCTTGCTG 601 TGCTGTTTTC CGTCCCCTAT GACTACAACT GGTATTCCAA CTGGTGGAAT GTGCGCATCT 661 ACAAGGGCAA ACGTAGAGCC GATCAACGCA TGTATGAGGA GCTTTACTAC AACCTGTCCC 721 CGTTTAGGGG CGACAATGGA TGGCACACAC GGAATCTCGG GTATGGCCTG AAGAGCAGAG 781 GCTTCATGAA CAGCAGTGGC CATGCCATCC TGGAGATTCA CGTGTCCAAG GCTGGTGGAG 841 GGTCAGGCGG CGGATCTGAA TTCGGAGGAG GAGGAGGAGG ATCCATGGTG AGCAAAGGGC 901 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGATCGAGC TGGACGGCGA CGTAACGGGC 961 CACAAGTTCA GCGTGTCCCG GCGAGGGCGA GGGCGATGCC ACCTATGGCA AGCTGACCCT 1021 GAGGTCCATC CTGCACACCG GGCAAGCCTG CCCGTTGCCC CTGACCCATC GTCGTGACCA 1081 CCCTTGAACA TACGACGTTG CCAGGTGGCT TCCAGTC // |
Primer: f1ori_R3 Region: T7 ter, 6xHis, EGFP 3' Sequence file: RDB15961_A8E2b.seq ![]() |
>13496_15961_A8E2_3_f1ori_R3_B01_02_ABI08.ab1 1 AGATTTAGCC ATCCGGGGAA TTAGTTCCTC CTTTCAGCAA AAAACCCCTC AAGACCCGTT 61 TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT 121 CCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGA AGCTTCTTGT 181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT 241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT 301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT 361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT 421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA 481 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT 541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG 601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT 661 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC 721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCATAGGTGG 781 CATCGCCCTC GCCCTCGCCG GACACGGCTG AAACTTGTGG CCGTTTACGT CGCCGTCCAG 841 CTCGACCAGG ATGGGCACCA CCCCCGGGTG AAACAGCTCC TCGCCCTTGC TCACCATGGG 901 ATCCTCCTTC CTCCCTCCTC CCGAAATTCA GATCCGCCCG CCCTGGACCC TCCACCCAGC 961 CCTTTGGGAC ACGGTGAAAT CTCCAGGGAT GGGCATGGGC CACTGGCTGG TTCATGGAAG 1021 CCTCTGCCTC TTCAGGCCAG TAACCCCGAG AATTTCCGGT GGTGGTGCCC ATCCCATTGT 1081 CCGGCCCCCA TAATGGGGGG ACAAGGGTTG GTAGGTAAAG CTTCTAATAC ATGCCGGTGG 1141 GATCCGGCCC TCTATCGGGT TATTGGTCAC CTA // |
Primer: Tet-r-M_R Region: lacI Sequence file: RDB15961_A8E2c.seq ![]() |
>13496_15961_A8E2_3_Tet-r-M_R_H02_23_ABI24.ab1 1 GTGCCTAAAA AGTGGGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 61 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 121 TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG 181 CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC 241 AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA 301 TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG 361 CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC 421 TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT 481 TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA 541 CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG 601 ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG 661 GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA 721 GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG 781 AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC 841 ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC 901 GCGTGCAGGG CCAGACTGGA TGTGGCAACG CCCATCAGCA ACGACTGTTT GCCCGCCAGT 961 GGTGTGCCAC GCGATTGGGA TGTAATCAGC CTCGCCATCG CGCATCACTT TTTTCCCGCG 1021 TTTCGCAGAA CGTGTCTTGC CTGGTTC // |
Primer: EGFP-M_R Region: EGFP 5', insert 3' Sequence file: RDB15961_A8E2d.seq ![]() |
>13496_15961_B0C4F_EGFP-M_R_H07_08_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG TGNNGGNNCT TGAGTTCNCC TTGNTGCCGT TCTTCTGCTT 61 GTCGGCCATG NTATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT 121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC 181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG 241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC 301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG 361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCAT AGGTGGCATC 421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC 481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG ATCCTCCTCC 541 TCCTCCTCCG AATTCANATC CGCCGCCTGA NCCTCCACCA GCCTTGGANA CGTGAATCTC 601 CNGGANGGCA TGGCCACTGC TGNNCATGAA GCCTCNGNTC NTCNNNNNTA NCCGANANTN 661 CGNGTGTGNN NTNNNTTGNC GCCCCTAAAC GGGGNNNGNN NGNANTAAAG CTNNNNNNAN 721 NTGCGTNGAT NNNNTCNACN TTNGNCNNNN NNNANGNNCA CANTNNNNNA NNNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.10.02
GNP_filter3_RDBDEP_html_200316.pl