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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/EqtII-EGFP-His6 (#RDB13496)

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.

Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
Comment Optimized for expression in E. coli
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
 
Sequence (full) RDB13496zzt02.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。

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RDB13496 pET28/EqtII-EGFP-His6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET28/EqtII-EGFP-His6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13496).

Reference section:

Makino, A., Abe, M., Murate, M., Inaba, T., Yilmaz, N., Hullin-Matsuda, F., Kishimoto, T., Schieber, N.L., Taguchi, T., Arai, H., Anderluh, G., Parton, R.G., Kobayashi, T., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15961_A8E2p1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-check-F (Pr0191)
Region: T7 pro,lacO,RBS,insert 5'(EqtII 5')
Sequence file: RDB15961_A8E2j.seq check
>RDB13496_15961_3_A8E2_pET-check-F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGN NNGCCNCCNN ACCCNCGCCG AANCNAGCGC
   61 TCNTGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
  121 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
  181 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
  241 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCGGT ACCATGAGCG
  301 CGGATGTGGC TGGTGCAGTC ATAGATGGCG CCTCTCTGAG TTTCGACATC CTCAAGACCG
  361 TGTTGGAAGC TCTGGGGAAT GTCAAACGGA AGATTGCCGT AGGAGTGGAC AACGAAAGCG
  421 GTAAGACATG GACTGCCCTG AATACGTACT TCAGGTCTGG AACCAGCGAC ATTGTTCTGC
  481 CCCACAAAGT CCCACATGGG AAAGCACTCC TGTACAATGG GCAGAAAGAT CGAGGACCTG
  541 TTGCGACTGG TGCAGTTGGG GTATTGGCCT ATCTCATGTC AGATGGCAAC ACCCTTGCTG
  601 TGCTGTTTTC CGTCCCCTAT GACTACAACT GGTATTCCAA CTGGTGGAAT GTGCGCATCT
  661 ACAAGGGCAA ACGTAGAGCC GATCAACGCA TGTATGAGGA GCTTTACTAC AACCTGTCCC
  721 CGTTTANGGG CGACAATGGA TGGCACACAC GGAATCTCGG GTATGGCCTG NAGANCAGAN
  781 GCTTCATGAN CAGCAGTGNC CNTGCCATCC TGGANATTCA CGTGTCCNAN GCTGGNGGAN
  841 GGTCANGCGG CGGATCTGAA NTCNGANGAN GANGANGANG NNCCATGGTG ANCNANGGNN
  901 NANGANCTGN TCACCGGGGN GGNGCCCNTC CNNNNCNANC TNNNNNGNNN NGTNNNNNNN
961 NNN
//
Primer: f1ori_R3 (Pr0142)
Region: T7 ter,6xHis,insert 3'(EGFP 3')
Sequence file: RDB15961_A8E2k.seq check
>13496_15961_A8E2_3_f1ori_R3_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC CTTTNNNNNN NNNNCCCCTC AAGANCCGTT
   61 NAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT
  121 CCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGA AGCTTCTTGT
  181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
  241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
  301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
  361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT
  421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCANC TTGTGCCCCA
  481 NGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
  541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTANTTGCC GTCGTCCTTG AAGAAGATGG
  601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
  661 GGTCGGGGTA NCGGCTGAAG CACTGCACGC CGTANGTCAG GGTGGTCACG ANGGTGGGCC
  721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGNCAGCTTG CNNTANGTGG
  781 CATCGCCCTC GCCCTCGCCN GANACNNNTG AANCTTGTGG CCGTTNACGT CNCCGTCCNN
  841 CTCGACNANN NNGGGCNNCA CCCCCGGGTG AANCAGCTCC TCGCCCTTGC TCACCATGGG
  901 ATCCNNNNNN NNNCNTNNTN NNNNAATTNN NATCCGCCNN NNNTNNANCC NNNNNCNNNN
  961 CCNTNGGNAN NNNNNNNANT CTNNNNGGAN NGGCANNGNN NNNNNNCTNN TNNANNNANN
1021 NCNNTNNNTN NNNNNNNNN
//
Primer: Tet-r-M_R (Pr0204)
Region: lacI
Sequence file: RDB15961_A8E2l.seq check
>13496_15961_A8E2_3_Tet-r-M_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCTGNNT GGGTNNNNNG NTCTCNAGGG
   61 CATCGGTCGA GATCCCGGTG NNNNNNNNNN NNNNNNNNTT ACATTAATTG CGTTGCGCTC
  121 ACTGCCCNNN NTCCNGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
  181 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA
  241 CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA
  301 CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC
  361 ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT ATCCGCACCA ACGCGCAGCC
  421 CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG
  481 CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC
  541 TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC
  601 AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT
  661 GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT TCATGGGAGA
  721 AAATAATACT GTTGATGGGT GTCTGGTCAG AGNCATCAAG AAATAACGCC GGAACATTAG
  781 TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CNGATAGTNA ATGATCAGCC
  841 CACTGNCGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCN ANGCCGCTNC
  901 NNTCTNCCNT CGACACCACN ACGCNNGNNN CCAGTTGNTN GGNNCNNNNN TTNATCGCCN
  961 CGNNNNNTNG CGNNNNNNCN NGCNNNNNCN NNNNGGATNN GNNNNNNNCN NANNNNNNNN
1021 NN
//
Primer: EGFP-M_R (Pr0743)
Region: insert mid(EGFP 5')
Sequence file: RDB15961_A8E2m.seq check
>13496_15961_B0C4F_EGFP-M_R_H07_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG TGNNGGNNCT TGAGTTCNCC TTGNTGCCGT TCTTCTGCTT
   61 GTCGGCCATG NTATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT
  121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC
  181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG
  241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC
  301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG
  361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCAT AGGTGGCATC
  421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
  481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG ATCCTCCTCC
  541 TCCTCCTCCG AATTCANATC CGCCGCCTGA NCCTCCACCA GCCTTGGANA CGTGAATCTC
  601 CNGGANGGCA TGGCCACTGC TGNNCATGAA GCCTCNGNTC NTCNNNNNTA NCCGANANTN
  661 CGNGTGTGNN NTNNNTTGNC GCCCCTAAAC GGGGNNNGNN NGNANTAAAG CTNNNNNNAN
  721 NTGCGTNGAT NNNNTCNACN TTNGNCNNNN NNNANGNNCA CANTNNNNNA NNNNNNNNNN
//
Primer: YFP_R (Pr0127)
Region: insert 5'(EGFP 5',EqtII 3'),RBS,lacO
Sequence file: RDB15961_A8E2n.seq check
>13496_15961_B2J3F_YFP_R_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGA NTTGTGGCCG TTTNCGTCGC CGTCCNGCTC
   61 GACCAGGATG GGCACCACCC CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGATCCTCC
  121 TCCTCCTCCT CCGAATTCAG ATCCGCCGCC TGACCCTCCA CCAGCCTTGG ACACGTGAAT
  181 CTCCAGGATG GCATGGCCAC TGCTGTTCAT GAAGCCTCTG CTCTTCAGGC CATACCCGAG
  241 ATTCCGTGTG TGCCATCCAT TGTCGCCCCT AAACGGGGAC AGGTTGTAGT AAAGCTCCTC
  301 ATACATGCGT TGATCGGCTC TACGTTTGCC CTTGTAGATG CGCACATTCC ACCAGTTGGA
  361 ATACCAGTTG TAGTCATAGG GGACGGAAAA CAGCACAGCA AGGGTGTTGC CATCTGACAT
  421 GAGATAGGCC AATACCCCAA CTGCACCAGT CGCAACAGGT CCTCGATCTT TCTGCCCATT
  481 GTACAGGAGT GCTTTCCCAT GTGGGACTTT GTGGGGCAGA ACAATGTCGC TGGTTCCAGA
  541 CCTGAAGTAC GTATTCAGGG CAGTCCATGT CTTACCGCTT TCGTTGTCCA CTCCTACGGC
  601 AATCTTCCGT TTGACATTCC CCAGAGCTTC CAACACGGTC TTGAGGATGT CGAAACTCAG
  661 AGAGGCGCCA TCTATGACTG CACCAGCCAC ATCCGCGCTC ATGGTACCGG TATATCTCCT
  721 TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAANTGTT ATCCGCTCAC AATTCCCCTA
  781 TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCTCGATC CTCTACGCCN GANGCATCGT
  841 GGCCGGCATC ACCGGCGCCN CANGTGCNGT TGCTGGCGCC NATATCGNCN ANNTCACCGA
  901 NNGGGAAGAT CGGGNNCGCC ACTNNGGGCT CNNGANCGCT NGNTTCGGCN NNGGTANGGT
961 GNNNNNNCCC NNNNNNCNGG GGGNNTGNTN GGGNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP]