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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/EqtII-EGFP-His6

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.

Catalog number RDB13496
Resource name pET28/EqtII-EGFP-His6
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
Comment Optimized for expression in E. coli
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Actinia equina Equinatoxin II cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
Actinia equina Equinatoxin II
    Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13496 pET28/EqtII-EGFP-His6 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15961_A8E2p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pET-check-F
            Region: T7 pro, lacO, RBS, insert, EGFP 5'
            Sequence file: RDB15961_A8E2a.seq check
            >RDB13496_15961_3_A8E2_pET-check-F_C01_03_ABI08.ab1
                1 GGTAAAGGGG TCCACAGTCC CGGCACGGGG CTGCCACCAT ACCCACGCCG AAACAAGCGC
               61 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
              121 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
              181 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
              241 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCGGT ACCATGAGCG
              301 CGGATGTGGC TGGTGCAGTC ATAGATGGCG CCTCTCTGAG TTTCGACATC CTCAAGACCG
              361 TGTTGGAAGC TCTGGGGAAT GTCAAACGGA AGATTGCCGT AGGAGTGGAC AACGAAAGCG
              421 GTAAGACATG GACTGCCCTG AATACGTACT TCAGGTCTGG AACCAGCGAC ATTGTTCTGC
              481 CCCACAAAGT CCCACATGGG AAAGCACTCC TGTACAATGG GCAGAAAGAT CGAGGACCTG
              541 TTGCGACTGG TGCAGTTGGG GTATTGGCCT ATCTCATGTC AGATGGCAAC ACCCTTGCTG
              601 TGCTGTTTTC CGTCCCCTAT GACTACAACT GGTATTCCAA CTGGTGGAAT GTGCGCATCT
              661 ACAAGGGCAA ACGTAGAGCC GATCAACGCA TGTATGAGGA GCTTTACTAC AACCTGTCCC
              721 CGTTTAGGGG CGACAATGGA TGGCACACAC GGAATCTCGG GTATGGCCTG AAGAGCAGAG
              781 GCTTCATGAA CAGCAGTGGC CATGCCATCC TGGAGATTCA CGTGTCCAAG GCTGGTGGAG
              841 GGTCAGGCGG CGGATCTGAA TTCGGAGGAG GAGGAGGAGG ATCCATGGTG AGCAAAGGGC
              901 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGATCGAGC TGGACGGCGA CGTAACGGGC
              961 CACAAGTTCA GCGTGTCCCG GCGAGGGCGA GGGCGATGCC ACCTATGGCA AGCTGACCCT
             1021 GAGGTCCATC CTGCACACCG GGCAAGCCTG CCCGTTGCCC CTGACCCATC GTCGTGACCA
            1081 CCCTTGAACA TACGACGTTG CCAGGTGGCT TCCAGTC
            //
            Primer: f1ori_R3
            Region: T7 ter, 6xHis, EGFP 3'
            Sequence file: RDB15961_A8E2b.seq check
            >13496_15961_A8E2_3_f1ori_R3_B01_02_ABI08.ab1
                1 AGATTTAGCC ATCCGGGGAA TTAGTTCCTC CTTTCAGCAA AAAACCCCTC AAGACCCGTT
               61 TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC AACTCAGCTT
              121 CCTTTCGGGC TTTGTTAGCA GCCGGATCTC AGTGGTGGTG GTGGTGGTGA AGCTTCTTGT
              181 ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT
              241 GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT
              301 CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT
              361 GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT
              421 GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA
              481 GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT
              541 CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG
              601 TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT
              661 GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC
              721 AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCATAGGTGG
              781 CATCGCCCTC GCCCTCGCCG GACACGGCTG AAACTTGTGG CCGTTTACGT CGCCGTCCAG
              841 CTCGACCAGG ATGGGCACCA CCCCCGGGTG AAACAGCTCC TCGCCCTTGC TCACCATGGG
              901 ATCCTCCTTC CTCCCTCCTC CCGAAATTCA GATCCGCCCG CCCTGGACCC TCCACCCAGC
              961 CCTTTGGGAC ACGGTGAAAT CTCCAGGGAT GGGCATGGGC CACTGGCTGG TTCATGGAAG
             1021 CCTCTGCCTC TTCAGGCCAG TAACCCCGAG AATTTCCGGT GGTGGTGCCC ATCCCATTGT
             1081 CCGGCCCCCA TAATGGGGGG ACAAGGGTTG GTAGGTAAAG CTTCTAATAC ATGCCGGTGG
            1141 GATCCGGCCC TCTATCGGGT TATTGGTCAC CTA
            //
            Primer: Tet-r-M_R
            Region: lacI
            Sequence file: RDB15961_A8E2c.seq check
            >13496_15961_A8E2_3_Tet-r-M_R_H02_23_ABI24.ab1
                1 GTGCCTAAAA AGTGGGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT
               61 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT
              121 TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG
              181 CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC
              241 AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA
              301 TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG
              361 CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC
              421 TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT
              481 TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA
              541 CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG
              601 ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG
              661 GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA
              721 GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG
              781 AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC
              841 ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC
              901 GCGTGCAGGG CCAGACTGGA TGTGGCAACG CCCATCAGCA ACGACTGTTT GCCCGCCAGT
              961 GGTGTGCCAC GCGATTGGGA TGTAATCAGC CTCGCCATCG CGCATCACTT TTTTCCCGCG
            1021 TTTCGCAGAA CGTGTCTTGC CTGGTTC
            //
            Primer: EGFP-M_R
            Region: EGFP 5', insert 3'
            Sequence file: RDB15961_A8E2d.seq check
            >13496_15961_B0C4F_EGFP-M_R_H07_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNG TGNNGGNNCT TGAGTTCNCC TTGNTGCCGT TCTTCTGCTT
               61 GTCGGCCATG NTATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT
              121 GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC
              181 CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG
              241 CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC
              301 GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG GTCACGAGGG TGGGCCAGGG
              361 CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC AGCTTGCCAT AGGTGGCATC
              421 GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
              481 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGG ATCCTCCTCC
              541 TCCTCCTCCG AATTCANATC CGCCGCCTGA NCCTCCACCA GCCTTGGANA CGTGAATCTC
              601 CNGGANGGCA TGGCCACTGC TGNNCATGAA GCCTCNGNTC NTCNNNNNTA NCCGANANTN
              661 CGNGTGTGNN NTNNNTTGNC GCCCCTAAAC GGGGNNNGNN NGNANTAAAG CTNNNNNNAN
              721 NTGCGTNGAT NNNNTCNACN TTNGNCNNNN NNNANGNNCA CANTNNNNNA NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl