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pFN21A HaloTag-TRPV4 (WT) (#RDB20677)

Expression vector of human TRPV4. HaloTag(R) 7 was fused to the N terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kuwashima, Y., Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name Halo-TRPV4 (WT)
Clone info. Expression vector of human TRPV4. HaloTag(R) 7 was fused to the N terminus.
Vector backbone pFN21A HaloTag® CMV Flexi® Vector (plasmid)
Selectable markers Ampicillin
Gene/insert name human TRPV4 cDNA.
Depositor|Developer Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Kuwashima, Y. et al., Biophys. J., 123(7), 867-884, 2024 ) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the RIKEN CPR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees tobe bound by the conditions of the limited use statement of the Promega Corporation.
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提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwashima, Y. et al., Biophys. J., 123(7), 867-884, 2024 )。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、理研CPRの許可を得ること。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB20677 pFN21A HaloTag-TRPV4 (WT) DNA solution

How to cite this biological resource

Materials & Methods section:

The pFN21A HaloTag-TRPV4 (WT) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20677).

Reference section:

Kuwashima, Y., Yanagawa, M., Maekawa, M., Abe, M., Sako, Y., Arita, M., TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane. Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kuwashima, Y., TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane. Biophys. J. 123 (7): 867-884 (2024). PMID 38433447. [PubMed] [Article] [RRC of NBRP]