Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 with mCherry.
Clone info. | Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 (domain 4 of theta toxin) of C. perfringens (ABG82518 (protein)) fused with mCherry at N-terminus. |
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Comment | Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry. Expression was confirmed by the depositor with microscopy. |
Vector backbone | pcDNA-DEST40 (plasmid) |
Size of vector backbone | 7.1 kb |
Selectable markers | Ampicillin |
Gene/insert name | Clostridium perfringens pfoA genomic DNA |
Depositor|Developer | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020). |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020)。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18944 | pDEST/mCherry-D4 | DNA solution |
Materials & Methods section:
The pDEST/mCherry-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18944). |
Reference section:
Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18944_B1Ctp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5'(mCherry 5' Sequence file: RDB18944_B1Cta.seq ![]() |
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>D07782A2_B1Ct_1_CMV_Forward_G07_19_ABI24.ab1 1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC 121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTATCAG GTACCATGGT GAGCAAGGGC 181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC 241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC 301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC 361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC 421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG 481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC 541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC 601 ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG 661 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGANGT CAAGACCACC 721 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC 781 ATCACCTCCC ACAACGAGGN CTACACCATC GTGGAACAGT ACGAACGCGC CGANGGCCGC 841 CACTCCACCG GCGGCNTGGN CGANCTGTAC AAGAAGCTTA ANGGAAAAAT AAACTTANAT 901 CATAGNGGAN CCTATGNTGC ACAGTTTGAA GTANCCTNNN NGNNNTTTCN TATGACNAAG 961 NANNNAANTG AAGTTTTNNC NCNTAAAANN NGGGNNNGNN ANNNNNNNNG NNNAANNNNN 1021 NNNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA, insert 3' (D4,mCherry) Sequence file: RDB18944_B1Ctb.seq ![]() |
>D07782A2_B1Ct_1_bGH_rev3_C10_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG NANNAACAGN NGGCTGGCAA CTAGAAGGCA 61 CAGTCGAGGC TGATCAGCGG GTTTAAACCT CGAGTTAATT GTAAGTAATA CTAGATCCAG 121 GGTATAAAGT TGTTCCCCAT ATTGAAACAT TTATATTATT TGTTAATGGA ACATCATATT 181 CACTTATAAC ATCTCTCCAC CATTCCCAAG CAAGGCCTGT ACACTCTCTT GCTTTTATTC 241 TTATATTTCT TGCATTAGCT TCAAGAGGTA TTACTGTTGA ATAGTGAGCT GTTTTATCTT 301 GATAATTTCC ATCCCATGTT TTATGAGTTA AAACTTCATT TCCTTCTTTG TCATATGAAA 361 CTTCATCCCA GGCTACTTCA AACTGTGCAA CATAGGCTCC ACTATGATCT AAGTTTATTT 421 TTCCCTTAAG CTTCTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG CCCTCGGCGC 481 GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AACTTGATGT 541 TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC CTTGTAGGTG GTCTTGACCT 601 CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG CTTGATCTCG CCCTTCAGGG 661 CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA GCCCATGGTC TTCTTCTGCA 721 TTACGGGGCC GTCGGANGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACTCGC 781 CGTCCTGCAN GGANGAGTCC TGGGTCACGG TCACCACGCC GCCGTCCTCG AAGTTCATCA 841 CGCGCTCNCA CTTGAAGCCC TCGGGGAANG ANAGCTTCAA GTANTCNGGG ANGTCNNCGG 901 GGNGCTTCAC GTNNNNCTNG GNNCCGTACA TGANNTNAGG GGNNAGGNNG NNCNNNGNNN 961 ANNNNNNGGG GNNNCCCTNN NNNNNNTTNN NCTNNNCNNN CTGGGNNNCN NNNNNNNNCN 1021 NNNNNNNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR Sequence file: RDB18944_B1Ctc.seq ![]() |
>D07782A2_B1Ct_1_SV40pro_ori_F_D10_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNGNN NNNTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT 61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT 121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG 181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG 241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA 541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG 601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG 721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACATAGCGTT GGCTACCCGT GANATTGCTG 841 AAGAGCTTGN CGGCGAATGG GCTGACCGCT NNCTCGNGCT TTACNGTATC GCCGCTNCNN 901 ATTCGCAGCG CATCGCCTTC TATCGCNTTC TTGACNANNN NNTCNNNNCG GGNNTCTGGG 961 NNNNNNCGAA ANGACNNANC NNNCNNNNNC NNNNCNNNNN NNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP] |
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