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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST/mCherry-D4 (#RDB18944)

Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 with mCherry.

Clone info. Marker of cholesterol-rich domain of the plasma membrane. Expression vector of perfringolysin theta toxin D4 (domain 4 of theta toxin) of C. perfringens (ABG82518 (protein)) fused with mCherry at N-terminus.
Comment Vector, amplified mCherry was cloned into expression vector pcDNA-DEST40, generating DEST40-mCherry. Expression was confirmed by the depositor with microscopy.
Vector backbone pcDNA-DEST40 (plasmid)
Size of vector backbone 7.1 kb
Selectable markers Ampicillin
Gene/insert name Clostridium perfringens pfoA genomic DNA
Depositor|Developer Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Mol. Cell. Biol., 32 (8): 1396-1407, 2020)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18944 pDEST/mCherry-D4 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST/mCherry-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18944).

Reference section:

Abe, M., Makino, A., Hullin-Matsuda, F., Kamijo, K., Ohno-Iwashita, Y., Hanada, K., Mizuno, H., Miyawaki, A., Kobayashi, T., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18944_B1Ctp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'(mCherry 5'
Sequence file: RDB18944_B1Cta.seq check
>D07782A2_B1Ct_1_CMV_Forward_G07_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTATCAG GTACCATGGT GAGCAAGGGC
  181 GAGGAGGATA ACATGGCCAT CATCAAGGAG TTCATGCGCT TCAAGGTGCA CATGGAGGGC
  241 TCCGTGAACG GCCACGAGTT CGAGATCGAG GGCGAGGGCG AGGGCCGCCC CTACGAGGGC
  301 ACCCAGACCG CCAAGCTGAA GGTGACCAAG GGTGGCCCCC TGCCCTTCGC CTGGGACATC
  361 CTGTCCCCTC AGTTCATGTA CGGCTCCAAG GCCTACGTGA AGCACCCCGC CGACATCCCC
  421 GACTACTTGA AGCTGTCCTT CCCCGAGGGC TTCAAGTGGG AGCGCGTGAT GAACTTCGAG
  481 GACGGCGGCG TGGTGACCGT GACCCAGGAC TCCTCCCTGC AGGACGGCGA GTTCATCTAC
  541 AAGGTGAAGC TGCGCGGCAC CAACTTCCCC TCCGACGGCC CCGTAATGCA GAAGAAGACC
  601 ATGGGCTGGG AGGCCTCCTC CGAGCGGATG TACCCCGAGG ACGGCGCCCT GAAGGGCGAG
  661 ATCAAGCAGA GGCTGAAGCT GAAGGACGGC GGCCACTACG ACGCTGANGT CAAGACCACC
  721 TACAAGGCCA AGAAGCCCGT GCAGCTGCCC GGCGCCTACA ACGTCAACAT CAAGTTGGAC
  781 ATCACCTCCC ACAACGAGGN CTACACCATC GTGGAACAGT ACGAACGCGC CGANGGCCGC
  841 CACTCCACCG GCGGCNTGGN CGANCTGTAC AAGAAGCTTA ANGGAAAAAT AAACTTANAT
  901 CATAGNGGAN CCTATGNTGC ACAGTTTGAA GTANCCTNNN NGNNNTTTCN TATGACNAAG
  961 NANNNAANTG AAGTTTTNNC NCNTAAAANN NGGGNNNGNN ANNNNNNNNG NNNAANNNNN
1021 NNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA, insert 3' (D4,mCherry)
Sequence file: RDB18944_B1Ctb.seq check
>D07782A2_B1Ct_1_bGH_rev3_C10_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGGG NANNAACAGN NGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACCT CGAGTTAATT GTAAGTAATA CTAGATCCAG
  121 GGTATAAAGT TGTTCCCCAT ATTGAAACAT TTATATTATT TGTTAATGGA ACATCATATT
  181 CACTTATAAC ATCTCTCCAC CATTCCCAAG CAAGGCCTGT ACACTCTCTT GCTTTTATTC
  241 TTATATTTCT TGCATTAGCT TCAAGAGGTA TTACTGTTGA ATAGTGAGCT GTTTTATCTT
  301 GATAATTTCC ATCCCATGTT TTATGAGTTA AAACTTCATT TCCTTCTTTG TCATATGAAA
  361 CTTCATCCCA GGCTACTTCA AACTGTGCAA CATAGGCTCC ACTATGATCT AAGTTTATTT
  421 TTCCCTTAAG CTTCTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG CCCTCGGCGC
  481 GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AACTTGATGT
  541 TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC CTTGTAGGTG GTCTTGACCT
  601 CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG CTTGATCTCG CCCTTCAGGG
  661 CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA GCCCATGGTC TTCTTCTGCA
  721 TTACGGGGCC GTCGGANGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACTCGC
  781 CGTCCTGCAN GGANGAGTCC TGGGTCACGG TCACCACGCC GCCGTCCTCG AAGTTCATCA
  841 CGCGCTCNCA CTTGAAGCCC TCGGGGAANG ANAGCTTCAA GTANTCNGGG ANGTCNNCGG
  901 GGNGCTTCAC GTNNNNCTNG GNNCCGTACA TGANNTNAGG GGNNAGGNNG NNCNNNGNNN
  961 ANNNNNNGGG GNNNCCCTNN NNNNNNTTNN NCTNNNCNNN CTGGGNNNCN NNNNNNNNCN
1021 NNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR
Sequence file: RDB18944_B1Ctc.seq check
>D07782A2_B1Ct_1_SV40pro_ori_F_D10_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
  181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
  241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
  301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
  361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
  421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
  481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
  541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
  601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
  661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG
  721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG
  781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG ACATAGCGTT GGCTACCCGT GANATTGCTG
  841 AAGAGCTTGN CGGCGAATGG GCTGACCGCT NNCTCGNGCT TTACNGTATC GCCGCTNCNN
  901 ATTCGCAGCG CATCGCCTTC TATCGCNTTC TTGACNANNN NNTCNNNNCG GGNNTCTGGG
961 NNNNNNCGAA ANGACNNANC NNNCNNNNNC NNNNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Abe, M., A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis. Mol. Cell. Biol. 32 (8): 1396-1407 (2012). PMID 22331463. [link to RRC of NBRP]