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pET28/His6-mCherry-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB13499
Resource name pET28/His6-mCherry-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Comment 169-297 AA mutant. Reference of DNA sequence, D85846.1.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name common brandling worm Lysenin cDNA Discosoma sp. mCherry cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
 
Sequence (full) RDB13499hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB13499_B1L6p1-2.pdf 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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RDB13499 pET28/His6-mCherry-NT-Lys DNA solution

How to cite this biological resource

Materials & Methods section:

The pET28/His6-mCherry-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13499).

Reference section:

Liang, H., Ma, X., Zhang, Y., Liu, Y., Liu, N., Zhang, W., Chen, J., Liu, B., Du, W., Liu, X., Yu, L., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP]
Makino, A., Abe, M., Murate, M., Inaba, T., Yilmaz, N., Hullin-Matsuda, F., Kishimoto, T., Schieber, N.L., Taguchi, T., Arai, H., Anderluh, G., Parton, R.G., Kobayashi, T., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Liang, H., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP]
original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP]
user_report Stauffer, B.B., Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci. Rep. 7 (1): 2931 (2017). PMID 28592822. [link to RRC of NBRP]