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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/His6-mCherry-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB13499
Resource name pET28/His6-mCherry-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Comment 169-297 AA mutant. Reference of DNA sequence, D85846.1.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name common brandling worm Lysenin cDNA Discosoma sp. mCherry cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
common brandling worm Lysenin
    Discosoma sp. mCherry

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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            Remarks
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
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            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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            RDB13499 pET28/His6-mCherry-NT-Lys DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pET28/His6-mCherry-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13499).

            Reference section:

            Liang, H., Ma, X., Zhang, Y., Liu, Y., Liu, N., Zhang, W., Chen, J., Liu, B., Du, W., Liu, X., Yu, L., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP]
            Makino, A., Abe, M., Murate, M., Inaba, T., Yilmaz, N., Hullin-Matsuda, F., Kishimoto, T., Schieber, N.L., Taguchi, T., Arai, H., Anderluh, G., Parton, R.G., Kobayashi, T., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13499_A6Cbp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp (Pr0261)
            Region: 6xHis,thrombin site,insert 5'(mCherry 5')
            Sequence file: RDB13499_A6Cbd.seq check
            >13499_13499_A6Cb_3_T7_20bp_F11_17.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTTG TTNNNCTTTA AGAAGGAGAN ATACCATGGG
               61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCGGTACCAT
              121 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
              181 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
              241 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
              301 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
              361 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
              421 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
              481 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
              541 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
              601 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
              661 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
              721 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
              781 CGCCGANGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAAGC TTATAATCCT
              841 TGNAAAAACA GAAATTCNAA TTAAGCATGC AGAAAGGAAN GANTACATGA CAGTCGTTTC
              901 NNGAAAAAGT TGGNCAGCNG CNNCNNNNGG ANNTAGCNAA CNTTTNCNNG TTNGTGCNNN
            961 NNNNNNN
            //
            Primer: T7 terminator (Pr0155)
            Region: insert 3'(NT-Lys,mCherry 3')
            Sequence file: RDB13499_A6Cbe.seq check
            >13499_13499_A6Cb_3_T7_terminator_G11_20.ab1
                1 NNNNNNNNNN NNNNNNNTNN TNNGCNGCCG GNNCTCNGTG GTGGTGGTGG TGGTGCTCGA
               61 GTTAACCAAC CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG
              121 TTGCCGGTCC ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA
              181 CTACGTCACC ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT
              241 CAGTACCCTG ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG
              301 AATACATGGT GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT
              361 TGAAAAGTTT GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA
              421 TGTACTCCTT CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATAAGCT
              481 TCTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC CTCGGCGCGT TCGTACTGTT
              541 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAA CTTGATGTTG ACGTTGTAGG
              601 CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT CTTGACCTCA GCGTCGTAGT
              661 GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC CTTCANGGCG CCGTCCTCGG
              721 GGTACATCCG CTCGGANGAN GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT
              781 CNGANGGGAA NTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG NCCTGCANGG
              841 NNNANTNCTG GGTCACGGTC ACCACGCCGC CNNCCTCNAA GTTCATCACG CGCNNCNNNN
              901 NNAAGCCNTC NGGGNANNNA NAGCTTNNNG NNNTCNGGNN NNTCNNCGGG GNNCNNNNNG
            961 TNNNNNNNNN N
            //
            Primer: pGL3_RV4 (Pr0335)
            Region: lacI 3'
            Sequence file: RDB13499_A6Cbf.seq check
            >13499_13499_A6Cb_3_pGL3_RV4_H11_23.ab1
                1 NNNNNNNNNN NNNNNNNGNT CNNNNGGCNT CGGTCGANNT CCCGGNGCCT AATGAGTGAG
               61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
              121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
              181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
              241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
              301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
              361 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
              421 CCATCTGATC GTTGGCAACC AGCATCGCAG NNGGAACGAT GCCCTCNTTC AGCATTTGCA
              481 NGGTTNGNTG AAAACCGGAN NTGGNACTNC ANNCNCCTTC CCGTNNCGCT NNCGGCTGAA
            541 TTNNNNTGNN ANNGNNNNAT TTNNGCCAGC CANCCNNANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Liang, H., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP]
            original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP]
            user_report Stauffer, B.B., Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci. Rep. 7 (1): 2931 (2017). PMID 28592822. [link to RRC of NBRP]

            2024.03.26

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