Resource data sheet
Prev.

pET28/His6-mCherry-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB13499
Resource name pET28/His6-mCherry-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
169-297 AA mutant. Reference of DNA sequence, D85846.1.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name common brandling worm Lysenin cDNA Discosoma sp. mCherry cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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RDB13499 pET28/His6-mCherry-NT-Lys DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13499_A6Cbp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp | Sequence: RDB13499_A6Cba.seq
>13499_13499_A6Cb_3_T7_20bp_F11_17.ab1
    1 TCCAAAATGT TCCCATCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG
   61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCGGTACCAT
  121 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
  181 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
  241 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
  301 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
  361 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
  421 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
  481 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
  541 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
  601 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
  661 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
  721 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
  781 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAAGC TTATAATCCT
  841 TGGAAAAACA GAAATTCGAA TTAAGCATGC AGAAAGGAAG GAGTACATGA CAGTCGTTTC
  901 AAGAAAAAGT TGGCCAGCTG CAACTCTTGG ACATAGCAAA CTTTTTCAAG TTTGTGCTCT
  961 ATGAAGATTT GGGGGGGGGA TTTCGAATTA AAACGCTGAA ACACCATGTA TTTCGGGCTA
 1021 TGAGTATGCC CTATTTCCTC TTGATCAAAG AAGTATCCTA CCTTTGAATC ATGTACTGAT
1081 ATTCGAACAG CGCTGGACAA TACGATAGTC AGTGCCGTCC ATTCGCG
//
Primer: T7_terminator | Sequence: RDB13499_A6Cbb.seq
>13499_13499_A6Cb_3_T7_terminator_G11_20.ab1
    1 AATACATCTT TCGGGCTTTG TTAGCAGCCG GATCTCAGTG GTGGTGGTGG TGGTGCTCGA
   61 GTTAACCAAC CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG
  121 TTGCCGGTCC ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA
  181 CTACGTCACC ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT
  241 CAGTACCCTG ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG
  301 AATACATGGT GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT
  361 TGAAAAGTTT GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA
  421 TGTACTCCTT CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATAAGCT
  481 TCTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC CTCGGCGCGT TCGTACTGTT
  541 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAA CTTGATGTTG ACGTTGTAGG
  601 CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT CTTGACCTCA GCGTCGTAGT
  661 GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC CTTCAGGGCG CCGTCCTCGG
  721 GGTACATCCG CTCGGAGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT
  781 CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG TCCTGCAGGG
  841 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT
  901 TGAAGCCCTC GGGGAAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG
  961 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA AGGGCAGGGG
 1021 GCCACCCCTT TGGTCACCTT CAGCTTGGTC GTCTGGGTGC CCTCGTAGGG ACGATCCCTC
1081 GATCCACGAT CCTCGGATCA TCGGAACCTT CGAGTGGACC CGATC
//
Primer: pGL3_RV4 | Sequence: RDB13499_A6Cbc.seq
>13499_13499_A6Cb_3_pGL3_RV4_H11_23.ab1
    1 GGAGGACGTG ACCTTGGGTT GAAGGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA
   61 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
  121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
  181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA
  241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA
  301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT
  361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG
  421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA
  481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA
  541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG
  601 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT
  661 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT
  721 GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG
  781 CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT
  841 TGTGCACCGG CCGCTTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC
  901 GCTGGGCACC CAGTTGATCG GGCGCGAGGA TTTAATCGCC GCGAACAATT TGCCGAACGG
  961 GCGCGTGCAG GGCCAGGACT GGGAGTGGGC AACGGCCAAA TCAGCACGAC TGTTTGGCCC
 1021 GCCAGTTTGT TGGTGCCACG GCGGTTGGGG AATGTAATTC AAGCCTCGGC ATTCGACCGC
1081 CATCCACTTT TTCCCGGCGT TTTCGCAGAA ACCGGTTGGC CCGTGGGTCT CTTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.
reference Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.
user report Stauffer, B.B., Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci. Rep. 7 (1): 2931 (2017). PMID 28592822.

2019.10.05

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