icon below as well as clone information before placing your order.
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
|
Other clones in our bank
External Database common brandling worm Lysenin Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015) |
|---|---|
| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] |
| Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。 |
|---|---|
| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| 備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13499 | pET28/His6-mCherry-NT-Lys | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pET28/His6-mCherry-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13499). |
Reference section:
| Liang, H., Ma, X., Zhang, Y., Liu, Y., Liu, N., Zhang, W., Chen, J., Liu, B., Du, W., Liu, X., Yu, L., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP] |
| Makino, A., Abe, M., Murate, M., Inaba, T., Yilmaz, N., Hullin-Matsuda, F., Kishimoto, T., Schieber, N.L., Taguchi, T., Arai, H., Anderluh, G., Parton, R.G., Kobayashi, T., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Visualization of Organelles (English text) |
|---|---|
| Featured content | Sphingolipid signaling pathway (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13499_A6Cbp1-2.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
|
Primer: T7_20bp (Pr0261) Region: 6xHis,thrombin site,insert 5'(mCherry 5') Sequence file: RDB13499_A6Cbd.seq
|
|---|
|
>13499_13499_A6Cb_3_T7_20bp_F11_17.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTTG TTNNNCTTTA AGAAGGAGAN ATACCATGGG 61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCGGTACCAT 121 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT 181 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG 241 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT 301 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC 361 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT 421 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG 481 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT 541 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC 601 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA 661 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA 721 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG 781 CGCCGANGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAAGC TTATAATCCT 841 TGNAAAAACA GAAATTCNAA TTAAGCATGC AGAAAGGAAN GANTACATGA CAGTCGTTTC 901 NNGAAAAAGT TGGNCAGCNG CNNCNNNNGG ANNTAGCNAA CNTTTNCNNG TTNGTGCNNN 961 NNNNNNN // |
|
Primer: T7 terminator (Pr0155) Region: insert 3'(NT-Lys,mCherry 3') Sequence file: RDB13499_A6Cbe.seq
|
|
>13499_13499_A6Cb_3_T7_terminator_G11_20.ab1 1 NNNNNNNNNN NNNNNNNTNN TNNGCNGCCG GNNCTCNGTG GTGGTGGTGG TGGTGCTCGA 61 GTTAACCAAC CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG 121 TTGCCGGTCC ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA 181 CTACGTCACC ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT 241 CAGTACCCTG ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG 301 AATACATGGT GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT 361 TGAAAAGTTT GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA 421 TGTACTCCTT CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATAAGCT 481 TCTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC CTCGGCGCGT TCGTACTGTT 541 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAA CTTGATGTTG ACGTTGTAGG 601 CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT CTTGACCTCA GCGTCGTAGT 661 GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC CTTCANGGCG CCGTCCTCGG 721 GGTACATCCG CTCGGANGAN GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT 781 CNGANGGGAA NTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG NCCTGCANGG 841 NNNANTNCTG GGTCACGGTC ACCACGCCGC CNNCCTCNAA GTTCATCACG CGCNNCNNNN 901 NNAAGCCNTC NGGGNANNNA NAGCTTNNNG NNNTCNGGNN NNTCNNCGGG GNNCNNNNNG 961 TNNNNNNNNN N // |
|
Primer: pGL3_RV4 (Pr0335) Region: lacI 3' Sequence file: RDB13499_A6Cbf.seq
|
|
>13499_13499_A6Cb_3_pGL3_RV4_H11_23.ab1 1 NNNNNNNNNN NNNNNNNGNT CNNNNGGCNT CGGTCGANNT CCCGGNGCCT AATGAGTGAG 61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA 181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC 241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT 301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA 361 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG 421 CCATCTGATC GTTGGCAACC AGCATCGCAG NNGGAACGAT GCCCTCNTTC AGCATTTGCA 481 NGGTTNGNTG AAAACCGGAN NTGGNACTNC ANNCNCCTTC CCGTNNCGCT NNCGGCTGAA 541 TTNNNNTGNN ANNGNNNNAT TTNNGCCAGC CANCCNNANN NNNNNNNN // |
Please visit 
Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Liang, H., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP] |
|---|---|
| original | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP] |
| user_report | Stauffer, B.B., Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci. Rep. 7 (1): 2931 (2017). PMID 28592822. [link to RRC of NBRP] |
2023.09.13
GNP_filter3_RDBDEP_html_230702.pl