Sequencing and PCR primers for primer information.
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
| Catalog number | RDB13499 |
|---|---|
| Resource name | pET28/His6-mCherry-NT-Lys |
| Clone info. | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. 169-297 AA mutant. Reference of DNA sequence, D85846.1. |
| Vector backbone | pET-28b (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Kan^r |
| Gene/insert name | common brandling worm Lysenin cDNA Discosoma sp. mCherry cDNA |
| Depositor | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Please contact us. UCSD's MTA is also required. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
| Remarks | Please contact us. UCSD's MTA is also required. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13499 | pET28/His6-mCherry-NT-Lys | DNA solution |
Electronic file
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Sphingolipid signaling pathway (English text) |
|---|---|
| Featured content | Organelle marker (English text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13499_A6Cbp1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | Sequence: RDB13499_A6Cba.seq |
|---|
|
>13499_13499_A6Cb_3_T7_20bp_F11_17.ab1 1 TCCAAAATGT TCCCATCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG 61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCGGTACCAT 121 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT 181 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG 241 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT 301 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC 361 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT 421 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG 481 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT 541 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC 601 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA 661 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA 721 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG 781 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAAGC TTATAATCCT 841 TGGAAAAACA GAAATTCGAA TTAAGCATGC AGAAAGGAAG GAGTACATGA CAGTCGTTTC 901 AAGAAAAAGT TGGCCAGCTG CAACTCTTGG ACATAGCAAA CTTTTTCAAG TTTGTGCTCT 961 ATGAAGATTT GGGGGGGGGA TTTCGAATTA AAACGCTGAA ACACCATGTA TTTCGGGCTA 1021 TGAGTATGCC CTATTTCCTC TTGATCAAAG AAGTATCCTA CCTTTGAATC ATGTACTGAT 1081 ATTCGAACAG CGCTGGACAA TACGATAGTC AGTGCCGTCC ATTCGCG // |
| Primer: T7_terminator | Sequence: RDB13499_A6Cbb.seq |
|
>13499_13499_A6Cb_3_T7_terminator_G11_20.ab1 1 AATACATCTT TCGGGCTTTG TTAGCAGCCG GATCTCAGTG GTGGTGGTGG TGGTGCTCGA 61 GTTAACCAAC CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG 121 TTGCCGGTCC ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA 181 CTACGTCACC ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT 241 CAGTACCCTG ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG 301 AATACATGGT GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT 361 TGAAAAGTTT GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA 421 TGTACTCCTT CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATAAGCT 481 TCTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC CTCGGCGCGT TCGTACTGTT 541 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAA CTTGATGTTG ACGTTGTAGG 601 CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT CTTGACCTCA GCGTCGTAGT 661 GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC CTTCAGGGCG CCGTCCTCGG 721 GGTACATCCG CTCGGAGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT 781 CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG TCCTGCAGGG 841 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT 901 TGAAGCCCTC GGGGAAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG 961 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA AGGGCAGGGG 1021 GCCACCCCTT TGGTCACCTT CAGCTTGGTC GTCTGGGTGC CCTCGTAGGG ACGATCCCTC 1081 GATCCACGAT CCTCGGATCA TCGGAACCTT CGAGTGGACC CGATC // |
| Primer: pGL3_RV4 | Sequence: RDB13499_A6Cbc.seq |
|
>13499_13499_A6Cb_3_pGL3_RV4_H11_23.ab1 1 GGAGGACGTG ACCTTGGGTT GAAGGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA 61 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA 241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA 301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT 361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG 421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA 481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA 541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG 601 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT 661 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT 721 GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG 781 CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT 841 TGTGCACCGG CCGCTTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC 901 GCTGGGCACC CAGTTGATCG GGCGCGAGGA TTTAATCGCC GCGAACAATT TGCCGAACGG 961 GCGCGTGCAG GGCCAGGACT GGGAGTGGGC AACGGCCAAA TCAGCACGAC TGTTTGGCCC 1021 GCCAGTTTGT TGGTGCCACG GCGGTTGGGG AATGTAATTC AAGCCTCGGC ATTCGACCGC 1081 CATCCACTTT TTCCCGGCGT TTTCGCAGAA ACCGGTTGGC CCGTGGGTCT CTTG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. |
|---|---|
| reference | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. |
| user report | Stauffer, B.B., Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci. Rep. 7 (1): 2931 (2017). PMID 28592822. |
2019.10.05
GNP_filter3_RDBDEP_html_190927.pl