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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/His6-mCherry-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB13499
Resource name pET28/His6-mCherry-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Comment 169-297 AA mutant. Reference of DNA sequence, D85846.1.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name common brandling worm Lysenin cDNA Discosoma sp. mCherry cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
common brandling worm Lysenin
    Discosoma sp. mCherry

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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            RDB13499 pET28/His6-mCherry-NT-Lys DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13499_A6Cbp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp
            Sequence file: RDB13499_A6Cba.seq check
            >13499_13499_A6Cb_3_T7_20bp_F11_17.ab1
                1 TCCAAAATGT TCCCATCTAG AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG
               61 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCGGTACCAT
              121 GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT
              181 GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG
              241 CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT
              301 CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC
              361 CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT
              421 GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG
              481 CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT
              541 GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC
              601 CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA
              661 GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA
              721 CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG
              781 CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGAAGC TTATAATCCT
              841 TGGAAAAACA GAAATTCGAA TTAAGCATGC AGAAAGGAAG GAGTACATGA CAGTCGTTTC
              901 AAGAAAAAGT TGGCCAGCTG CAACTCTTGG ACATAGCAAA CTTTTTCAAG TTTGTGCTCT
              961 ATGAAGATTT GGGGGGGGGA TTTCGAATTA AAACGCTGAA ACACCATGTA TTTCGGGCTA
             1021 TGAGTATGCC CTATTTCCTC TTGATCAAAG AAGTATCCTA CCTTTGAATC ATGTACTGAT
            1081 ATTCGAACAG CGCTGGACAA TACGATAGTC AGTGCCGTCC ATTCGCG
            //
            Primer: T7_terminator
            Sequence file: RDB13499_A6Cbb.seq check
            >13499_13499_A6Cb_3_T7_terminator_G11_20.ab1
                1 AATACATCTT TCGGGCTTTG TTAGCAGCCG GATCTCAGTG GTGGTGGTGG TGGTGCTCGA
               61 GTTAACCAAC CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG
              121 TTGCCGGTCC ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA
              181 CTACGTCACC ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT
              241 CAGTACCCTG ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG
              301 AATACATGGT GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT
              361 TGAAAAGTTT GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA
              421 TGTACTCCTT CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATAAGCT
              481 TCTTGTACAG CTCGTCCATG CCGCCGGTGG AGTGGCGGCC CTCGGCGCGT TCGTACTGTT
              541 CCACGATGGT GTAGTCCTCG TTGTGGGAGG TGATGTCCAA CTTGATGTTG ACGTTGTAGG
              601 CGCCGGGCAG CTGCACGGGC TTCTTGGCCT TGTAGGTGGT CTTGACCTCA GCGTCGTAGT
              661 GGCCGCCGTC CTTCAGCTTC AGCCTCTGCT TGATCTCGCC CTTCAGGGCG CCGTCCTCGG
              721 GGTACATCCG CTCGGAGGAG GCCTCCCAGC CCATGGTCTT CTTCTGCATT ACGGGGCCGT
              781 CGGAGGGGAA GTTGGTGCCG CGCAGCTTCA CCTTGTAGAT GAACTCGCCG TCCTGCAGGG
              841 AGGAGTCCTG GGTCACGGTC ACCACGCCGC CGTCCTCGAA GTTCATCACG CGCTCCCACT
              901 TGAAGCCCTC GGGGAAAGGA CAGCTTCAAG TAGTCGGGGA TGTCGGCGGG GTGCTTCACG
              961 TAGGCCTTGG AGCCGTACAT GAACTGAGGG GACAGGATGT CCCAGGCGAA AGGGCAGGGG
             1021 GCCACCCCTT TGGTCACCTT CAGCTTGGTC GTCTGGGTGC CCTCGTAGGG ACGATCCCTC
            1081 GATCCACGAT CCTCGGATCA TCGGAACCTT CGAGTGGACC CGATC
            //
            Primer: pGL3_RV4
            Sequence file: RDB13499_A6Cbc.seq check
            >13499_13499_A6Cb_3_pGL3_RV4_H11_23.ab1
                1 GGAGGACGTG ACCTTGGGTT GAAGGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA
               61 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA
              121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
              181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA
              241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA
              301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT
              361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG
              421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA
              481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA
              541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG
              601 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT
              661 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT
              721 GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG
              781 CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT
              841 TGTGCACCGG CCGCTTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC
              901 GCTGGGCACC CAGTTGATCG GGCGCGAGGA TTTAATCGCC GCGAACAATT TGCCGAACGG
              961 GCGCGTGCAG GGCCAGGACT GGGAGTGGGC AACGGCCAAA TCAGCACGAC TGTTTGGCCC
             1021 GCCAGTTTGT TGGTGCCACG GCGGTTGGGG AATGTAATTC AAGCCTCGGC ATTCGACCGC
            1081 CATCCACTTT TTCCCGGCGT TTTCGCAGAA ACCGGTTGGC CCGTGGGTCT CTTG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.
            user_report Stauffer, B.B., Bacterial Sphingomyelinase is a State-Dependent Inhibitor of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR). Sci. Rep. 7 (1): 2931 (2017). PMID 28592822.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl