Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/His6-EGFP-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
Ordering	Please visit Information of Request for Distribution.  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

Distribution information (domestic users) [open/close]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13498_A5I3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB13498_A5I3a.seq
>13498_13498_A5I3_4_T7_20bp_E05_14.ab1     1 TCATGTTCCA TCTAGAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA    61 GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGAG   121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC   361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA   601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC   661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC   781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGAAGCTTA   841 TAATCCTTGG AAAAACAGAA ATTCGAATTA AGCATGCAGA AAGGAAGGAG TACATGACAG   901 TCGTTTCAAG AAAAAAGTTG GCCAGCTGCA ACTCTTGGAC ATAGCAAACT TTTTCAAGTT   961 TGTGCTCTAT GAAGATTGGG GGGGGGGATT TCGAAATTAA AACGCTGAAC ACCCATGTAT  1021 TTCGGGCTAA TGAGTAATGC CCTATTCCCT CCTGATCATG ATGCAATCTA CTTTGGATTC 1081 ATGGTTACCT GATTAATCCG TAAACAGTCG CTGGCAGTCA TAGTTCATTG ATCCAGC //
Primer: T7_terminator Sequence file: RDB13498_A5I3b.seq
>13498_13498_A5I3_4_T7_terminator_F05_17.ab1     1 ACTAACGTTC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC    61 GAGTTAACCA ACCACTTCCA AAATCCACTT GTCTTCACGT TTGTCCAGAC AGTACACGTT   121 TGTTGCCGGT CCATCATCGT AGCACAGCCC ACTGCGAGTG AAGTACTTAT TCATGAAGGT   181 GACTACGTCA CCATGACGAA GAGGCAATGA CTTATTGATT GCCCAGCGCT GTTTCGGATT   241 ATCAGTACCC TGATCAAAGT AGATTCCTCC TTGATCAGAG GAATAGGCAT ACTCATAGCC   301 CGAATACATG GTGTTCAGCG TTTTAATTCG AAATCCCCCC CAATCTTCAT AGAGCACAAA   361 CTTGAAAAGT TTGCTATGTC CAAGAGTTGC AGCTGGCCAA CTTTTTCTTG AAACGACTGT   421 CATGTACTCC TTCCTTTCTG CATGCTTAAT TCGAATTTCT GTTTTTCCAA GGATTATAAG   481 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG   541 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA   601 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC   661 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC   721 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT   781 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC   841 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA   901 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAAG AAGTCGTGCT   961 GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC CGTAGGTCAG GTGGTCACGA  1021 GGTGGGCCCA GGCACGGCAG CTTGCCCGGT GGTTGCAGAT GACTTTCAGG GTCAGCTTGC 1081 GTAAGTTGCC AATCGACCCT TCGATCCCTC GCCGGAACAA CGCCTTGAAC CATAG //
Primer: pGL3_RV4 Sequence file: RDB13498_A5I3c.seq
>13498_13498_A5I3_4_pGL3_Rv4_G05_20.ab1     1 CAAAAGGCAT GGGTTGAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG    61 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG   121 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC   181 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG   241 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG   301 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC   361 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG   421 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG   481 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG   541 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA   601 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC   661 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA   721 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG   781 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC   841 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC   901 CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG   961 ACTGGAGGTG GCAAACGCCA AATCAGCAAC GACTGTTTGC CCGCCCAGTT TGTTGTGCCA  1021 CGCGGGTTGG GAATGTAAAT TTCAGCTCCG CCAATCGACG CCTTCACTTT TTCCCGCGAT 1081 TTCGCAGATC GTGGCTGGAC CCTGGAATTC CACCCACCGC CGGGGAAAAA AC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.03.02

GNP_filter3_RDBDEP_html_191130.pl