Sequencing and PCR primers for primer information.
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
| Catalog number | RDB13498 |
|---|---|
| Resource name | pET28/His6-EGFP-NT-Lys |
| Clone info. | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. 169-297 AA mutant. Reference of DNA sequence, D85846.1. |
| Vector backbone | pET-28b (Plasmid) |
| Size of vector backbone | 5.4 kb |
| Selectable markers | Kan^r |
| Gene/insert name | common brandling worm Lysenin cDNA Aequorea victoria GFP cDNA |
| Depositor | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015) |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13498 | pET28/His6-EGFP-NT-Lys | DNA solution |
Electronic file
Featured content
| Featured content | Sphingolipid signaling pathway (English text) |
|---|---|
| Featured content | Organelle marker (English text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13498_A5I3p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: T7_20bp | RDB13498_A5I3a.seq |
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>13498_13498_A5I3_4_T7_20bp_E05_14.ab1 1 TCATGTTCCA TCTAGAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA 61 GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGAG 121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG 181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA 241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG 301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC 361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA 421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA 481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC 541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA 601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC 661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC 721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC 781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGAAGCTTA 841 TAATCCTTGG AAAAACAGAA ATTCGAATTA AGCATGCAGA AAGGAAGGAG TACATGACAG 901 TCGTTTCAAG AAAAAAGTTG GCCAGCTGCA ACTCTTGGAC ATAGCAAACT TTTTCAAGTT 961 TGTGCTCTAT GAAGATTGGG GGGGGGGATT TCGAAATTAA AACGCTGAAC ACCCATGTAT 1021 TTCGGGCTAA TGAGTAATGC CCTATTCCCT CCTGATCATG ATGCAATCTA CTTTGGATTC 1081 ATGGTTACCT GATTAATCCG TAAACAGTCG CTGGCAGTCA TAGTTCATTG ATCCAGC // |
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| Primer: T7_terminator | RDB13498_A5I3b.seq |
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>13498_13498_A5I3_4_T7_terminator_F05_17.ab1 1 ACTAACGTTC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC 61 GAGTTAACCA ACCACTTCCA AAATCCACTT GTCTTCACGT TTGTCCAGAC AGTACACGTT 121 TGTTGCCGGT CCATCATCGT AGCACAGCCC ACTGCGAGTG AAGTACTTAT TCATGAAGGT 181 GACTACGTCA CCATGACGAA GAGGCAATGA CTTATTGATT GCCCAGCGCT GTTTCGGATT 241 ATCAGTACCC TGATCAAAGT AGATTCCTCC TTGATCAGAG GAATAGGCAT ACTCATAGCC 301 CGAATACATG GTGTTCAGCG TTTTAATTCG AAATCCCCCC CAATCTTCAT AGAGCACAAA 361 CTTGAAAAGT TTGCTATGTC CAAGAGTTGC AGCTGGCCAA CTTTTTCTTG AAACGACTGT 421 CATGTACTCC TTCCTTTCTG CATGCTTAAT TCGAATTTCT GTTTTTCCAA GGATTATAAG 481 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG 541 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA 601 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC 661 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC 721 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT 781 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC 841 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA 901 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAAG AAGTCGTGCT 961 GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC CGTAGGTCAG GTGGTCACGA 1021 GGTGGGCCCA GGCACGGCAG CTTGCCCGGT GGTTGCAGAT GACTTTCAGG GTCAGCTTGC 1081 GTAAGTTGCC AATCGACCCT TCGATCCCTC GCCGGAACAA CGCCTTGAAC CATAG // |
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| Primer: pGL3_RV4 | RDB13498_A5I3c.seq |
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>13498_13498_A5I3_4_pGL3_Rv4_G05_20.ab1 1 CAAAAGGCAT GGGTTGAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG 61 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG 121 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC 181 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG 241 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG 301 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC 361 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG 421 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG 481 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG 541 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA 601 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC 661 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA 721 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG 781 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC 841 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC 901 CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG 961 ACTGGAGGTG GCAAACGCCA AATCAGCAAC GACTGTTTGC CCGCCCAGTT TGTTGTGCCA 1021 CGCGGGTTGG GAATGTAAAT TTCAGCTCCG CCAATCGACG CCTTCACTTT TTCCCGCGAT 1081 TTCGCAGATC GTGGCTGGAC CCTGGAATTC CACCCACCGC CGGGGAAAAA AC // |
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Please visit Sequencing and PCR primers for primer information.
References
2019.03.11
GNP_filter3_RDBDEP_html_190307.pl