Resource data sheet
Prev.

pET28/His6-EGFP-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Catalog number RDB13498
Resource name pET28/His6-EGFP-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
169-297 AA mutant. Reference of DNA sequence, D85846.1.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name common brandling worm Lysenin cDNA Aequorea victoria GFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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Schedule A (GFP-A) [Link]
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RDB13498 pET28/His6-EGFP-NT-Lys DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

References

original Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303.
original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13498_A5I3p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13498_A5I3a.seq
>13498_13498_A5I3_4_T7_20bp_E05_14.ab1
    1 TCATGTTCCA TCTAGAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA
   61 GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGAG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGAAGCTTA
  841 TAATCCTTGG AAAAACAGAA ATTCGAATTA AGCATGCAGA AAGGAAGGAG TACATGACAG
  901 TCGTTTCAAG AAAAAAGTTG GCCAGCTGCA ACTCTTGGAC ATAGCAAACT TTTTCAAGTT
  961 TGTGCTCTAT GAAGATTGGG GGGGGGGATT TCGAAATTAA AACGCTGAAC ACCCATGTAT
 1021 TTCGGGCTAA TGAGTAATGC CCTATTCCCT CCTGATCATG ATGCAATCTA CTTTGGATTC
1081 ATGGTTACCT GATTAATCCG TAAACAGTCG CTGGCAGTCA TAGTTCATTG ATCCAGC
//
Primer: T7_terminator RDB13498_A5I3b.seq
>13498_13498_A5I3_4_T7_terminator_F05_17.ab1
    1 ACTAACGTTC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC
   61 GAGTTAACCA ACCACTTCCA AAATCCACTT GTCTTCACGT TTGTCCAGAC AGTACACGTT
  121 TGTTGCCGGT CCATCATCGT AGCACAGCCC ACTGCGAGTG AAGTACTTAT TCATGAAGGT
  181 GACTACGTCA CCATGACGAA GAGGCAATGA CTTATTGATT GCCCAGCGCT GTTTCGGATT
  241 ATCAGTACCC TGATCAAAGT AGATTCCTCC TTGATCAGAG GAATAGGCAT ACTCATAGCC
  301 CGAATACATG GTGTTCAGCG TTTTAATTCG AAATCCCCCC CAATCTTCAT AGAGCACAAA
  361 CTTGAAAAGT TTGCTATGTC CAAGAGTTGC AGCTGGCCAA CTTTTTCTTG AAACGACTGT
  421 CATGTACTCC TTCCTTTCTG CATGCTTAAT TCGAATTTCT GTTTTTCCAA GGATTATAAG
  481 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG
  541 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA
  601 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC
  661 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC
  721 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT
  781 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC
  841 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA
  901 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAAG AAGTCGTGCT
  961 GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC CGTAGGTCAG GTGGTCACGA
 1021 GGTGGGCCCA GGCACGGCAG CTTGCCCGGT GGTTGCAGAT GACTTTCAGG GTCAGCTTGC
1081 GTAAGTTGCC AATCGACCCT TCGATCCCTC GCCGGAACAA CGCCTTGAAC CATAG
//
Primer: pGL3_RV4 RDB13498_A5I3c.seq
>13498_13498_A5I3_4_pGL3_Rv4_G05_20.ab1
    1 CAAAAGGCAT GGGTTGAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG
   61 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
  121 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
  181 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
  241 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
  301 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
  361 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
  421 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
  481 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
  541 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
  601 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
  661 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  721 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
  781 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
  841 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC
  901 CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG
  961 ACTGGAGGTG GCAAACGCCA AATCAGCAAC GACTGTTTGC CCGCCCAGTT TGTTGTGCCA
 1021 CGCGGGTTGG GAATGTAAAT TTCAGCTCCG CCAATCGACG CCTTCACTTT TTCCCGCGAT
1081 TTCGCAGATC GTGGCTGGAC CCTGGAATTC CACCCACCGC CGGGGAAAAA AC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303.
original Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132.

2019.03.11

GNP_filter3_RDBDEP_html_190307.pl