Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/His6-EGFP-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13498_A6Cap1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Region: 6xHis,thrombin site,insert 5'(EGFP 5') Sequence file: RDB13498_A6Cad.seq
>13498_13498_A6Ca_4_T7_20bp_E05_14.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTTGTTNNN CTTTAAGAAG GAGNNATACC ATGGGCAGCA    61 GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGAG   121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC   361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA   601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC   661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   721 TGANCACCCA GTCCGCCCTG ANCAAAGANC CCAACGAGAA GCGCGATCAC ATGGTCCTGC   781 TGGANTTCGT GACCGCCGCC GGGATCACTC TCGGCATGNA CGANCTGTAC AAGAAGCTTA   841 TAATCCTTGG AAAANCAGAA NNTCGAANTN AGCANGCAGA AANGAANGNN TACATGACAG   901 TCGTTNCNAG AAAAAAGTTN GCCAGCNGCN NNNNTTGGNN ATANCAAACT TTTTCNNNNT 961 NGNGNNNNAN NNNNNNNNN //
Primer: T7 terminator Region: insert 3'(NT-Lys, EGFP 3') Sequence file: RDB13498_A6Cae.seq
>13498_13498_A6Ca_4_T7_terminator_F05_17.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNAGCAGC CGGNNCTCAG TGGTGGTGGT GGTGGTGCTC    61 GAGTTAACCA ACCACTTCCA AAATCCACTT GTCTTCACGT TTGTCCAGAC AGTACACGTT   121 TGTTGCCGGT CCATCATCGT AGCACAGCCC ACTGCGAGTG AAGTACTTAT TCATGAAGGT   181 GACTACGTCA CCATGACGAA GAGGCAATGA CTTATTGATT GCCCAGCGCT GTTTCGGATT   241 ATCAGTACCC TGATCAAAGT AGATTCCTCC TTGATCAGAG GAATAGGCAT ACTCATAGCC   301 CGAATACATG GTGTTCAGCG TTTTAATTCG AAATCCCCCC CAATCTTCAT AGAGCACAAA   361 CTTGAAAAGT TTGCTATGTC CAAGAGTTGC AGCTGGCCAA CTTTTTCTTG AAACGACTGT   421 CATGTACTCC TTCCTTTCTG CATGCTTAAT TCGAATTTCT GTTTTTCCAA GGATTATAAG   481 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG   541 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA   601 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC   661 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC   721 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT   781 GTGCCCCANG ATGTTGCCGT CCTCCTTGAA GTCNATGCNN TTCAGCTCGA TGCGGTTCAC   841 CAGGGTGTCG CCCTCGAACT TCACNTCGGC GCNGGTCNTG TAGTTGNCGT CGTNCNNNAA   901 GAANANGGTG CGCNNCNNGN NGTANCCNNC NGGNNTGGNG GNNTNGNANN AANNCGTGCT 961 GCTNNNTGTN NNCGGGNNAN CGGNNGNNNN NNNNNN //
Primer: T7 terminator Region: insert 3'(NT-Lys, EGFP 3') Sequence file: RDB13498_A6Cae.seq
>13498_13498_A6Ca_4_T7_terminator_F05_17.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNAGCAGC CGGNNCTCAG TGGTGGTGGT GGTGGTGCTC    61 GAGTTAACCA ACCACTTCCA AAATCCACTT GTCTTCACGT TTGTCCAGAC AGTACACGTT   121 TGTTGCCGGT CCATCATCGT AGCACAGCCC ACTGCGAGTG AAGTACTTAT TCATGAAGGT   181 GACTACGTCA CCATGACGAA GAGGCAATGA CTTATTGATT GCCCAGCGCT GTTTCGGATT   241 ATCAGTACCC TGATCAAAGT AGATTCCTCC TTGATCAGAG GAATAGGCAT ACTCATAGCC   301 CGAATACATG GTGTTCAGCG TTTTAATTCG AAATCCCCCC CAATCTTCAT AGAGCACAAA   361 CTTGAAAAGT TTGCTATGTC CAAGAGTTGC AGCTGGCCAA CTTTTTCTTG AAACGACTGT   421 CATGTACTCC TTCCTTTCTG CATGCTTAAT TCGAATTTCT GTTTTTCCAA GGATTATAAG   481 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG   541 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA   601 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC   661 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC   721 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT   781 GTGCCCCANG ATGTTGCCGT CCTCCTTGAA GTCNATGCNN TTCAGCTCGA TGCGGTTCAC   841 CAGGGTGTCG CCCTCGAACT TCACNTCGGC GCNGGTCNTG TAGTTGNCGT CGTNCNNNAA   901 GAANANGGTG CGCNNCNNGN NGTANCCNNC NGGNNTGGNG GNNTNGNANN AANNCGTGCT 961 GCTNNNTGTN NNCGGGNNAN CGGNNGNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl