Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
Drawn by SnapGene® software |
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Clone info. | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
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Comment | 169-297 AA mutant. Reference of DNA sequence, D85846.1. |
Vector backbone | pET-28b (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Kan^r |
Gene/insert name | common brandling worm Lysenin cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13498 | pET28/His6-EGFP-NT-Lys | DNA solution |
Materials & Methods section:
The pET28/His6-EGFP-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13498). |
Reference section:
Makino, A., Abe, M., Murate, M., Inaba, T., Yilmaz, N., Hullin-Matsuda, F., Kishimoto, T., Schieber, N.L., Taguchi, T., Arai, H., Anderluh, G., Parton, R.G., Kobayashi, T., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Makino, A., Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis. FASEB J. 29 (2): 477-493 (2015). PMID 25389132. [link to RRC of NBRP] |
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protocol | Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303. |
review | Kobayashi, T., Impact of Intrinsic and Extrinsic Factors on Cellular Sphingomyelin Imaging with Specific Reporter Proteins. |
user_report | Liang, H., The formation of migrasomes is initiated by the assembly of sphingomyelin synthase 2 foci at the leading edge of migrating cells. Nat. Cell Biol. 25 (8): 1173-1184 (2023). PMID 37488437. [link to RRC of NBRP] |
user_report | Mori, Y., Membrane Sphingomyelin in Host Cells Is Essential for Nucleocapsid Penetration into the Cytoplasm after Hemifusion during Rubella Virus Entry. mBio 13 (6): e0169822 (2022). PMID 36346228. [link to RRC of NBRP] |
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