Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.
Clone info. | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2. |
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Comment | Expression was confirmed by the depositor. |
Vector backbone | pET-28b (Plasmid) |
Size of vector backbone | 5.4 kb |
Selectable markers | Km^r |
Gene/insert name | Pleurotus eryngii PlyA2 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Sequence (full) ![]() |
RDB13962zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., J Lipid Res. 54 (10): 2933-2943, 2013.) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Bhat, HB., J Lipid Res. 54 (10): 2933-2943, 2013.)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13962 | pET28/PlyA2-EGFP-His6 | DNA solution |
Materials & Methods section:
The pET28/PlyA2-EGFP-His6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13962). |
Reference section:
Bhat, H.B., Ishitsuka, R., Inaba, T., Murate, M., Abe, M., Makino, A., Kohyama-Koganeya, A., Nagao, K., Kurahashi, A., Kishimoto, T., Tahara, M., Yamano, A., Nagamune, K., Hirabayashi, Y., Juni, N., Umeda, M., Fujimori, F., Nishibori, K., Yamaji-Hasegawa, A., Greimel, P., Kobayashi, T., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. [link to RRC of NBRP] |
Bhat, H.B., Kishimoto, T., Abe, M., Makino, A., Inaba, T., Murate, M., Dohmae, N., Kurahashi, A., Nishibori, K., Fujimori, F., Greimel, P., Ishitsuka, R., Kobayashi, T., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13962_A6Btp1-3.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pBR322_BamHI_R (Pr0065) Region: RBS, insert 5' (PlyA2, EGFP 5') Sequence file: RDB13962_A6Btd.seq ![]() |
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>D03144A5_A6Bt_1_pBR 322 BamHI R_D04_10.ab1 1 NNNNNNNNNN NNNGNNTNNN NNTANGGNNN NTGTGAGCGG NNAACAATTC CCCTCTAGAA 61 ATAATTTTGT TNAACTTTAA GAAGGAGATA TACCGGTACC ATGGCATACG CACAATGGGT 121 CATCATCATC ATCCACAACG TCGGGTCCAA AGACGTGAAG ATCGTAAACC TCAAGCCTTC 181 TTGGGGGAAG CTTCATGCCG ACGGCGACAA AGACACAGAA GTCTCAGCAA GCAAGTACGA 241 AGGAACAGTC ATCAAGCCTG ACGAGAAGCT CCAGATCAAT GCCTGCGGCA GATCAGACGC 301 CGCCGAAGGC ACGACAGGAA CCTTCGACCT TGTTGACCCC GCCGATGGCG ACAAGCAGGT 361 CCGCCACTTC TACTGGGACT GCCCATGGGG AAGCAAGGCC AACACTTGGA CCGTTAGCGG 421 TTCCAACACC AAGTGGATGA TCGAGTACTC TGGCCAGAAC TTGGACAGCG GTGCCCTCGG 481 CACGATCACC GTCGACACCT TGAAGAAGGG GAACGAATTC GGAGGAGGAG GAGGAGGATC 541 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA 601 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA 661 TGGCAAGCTG ANCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC 721 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA 781 GCAGCACGAC TTCTTCNAGT CCGCCATGCC CGAANGCTAC GTCCAGGANC GCANCATCTT 841 CTTCNNNGAC GANNGNNACT ACAAGANCCG CGCCNANGTG NANTTCGNNG GCGANNCCCN 901 GNNNNNNCNC ATCNAGCTGN ANNGNNNTCN NNTNNNNNNN NNANNNNNNC ATNCNGGGGN 961 ANNNGCTGGN NTNNNNCTNN NNNNNNNN // |
Primer: EGFP-N_F (Pr0649) Region: insert 3' (EGFP 3'), 6xHis Sequence file: RDB13962_A6Bte.seq ![]() |
>13962_13962_B2D1F_1_EGFP-N_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NGNNNNNNNG NNGGCGNNGC NNCCTATGGC AAGCTGNNCC TGAAGTTCAT 61 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG 121 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC 181 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA 241 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 301 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG 361 CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT 421 CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC 481 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT 541 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC 601 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAAGCTT CACCACCACC ACCACCACTG 661 AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGANTTG GCTGCTGCCA CCGCTGAGCA 721 ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGANAGG 781 AGGAACTATA TCCGGANTGN CGAATGGGAC GCGCCCNGTA GCGGCGCATT AAGCGCGGCG 841 GGTGTGGTGG TTACGCGCAG CGNGACCGCT ACACTTGCCA GCGCCCTANC GCCCGCTNCT 901 TTCGCTTTCN NCCCTTNCTT NCNCGCNNCN TNCGCCNGCT TNCCCCGNNN NGCTCNAANN 961 CNGGGGNNNN CNNTNNNGNN NNNNNNTNNN NNNNTNNNNN NNNNN // |
Primer: EGFP-M_R (Pr0743) Region: insert mid (EGFP 5', PlyA2 3') Sequence file: RDB13962_A6Btf.seq ![]() |
>13962_13962_B2D1F_1_EGFP-M_R_A12_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGCGGNNC TNNNNNNNNC CTTGATGCCG TTCTTCTGCT 61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA 121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC 181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC 241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT 301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG 361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCA TAGGTGGCAT 421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA 481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GATCCTCCTC 541 CTCCTCCTCC GAATTCGTTC CCCTTCTTCA AGGTGTCGAC GGTGATCGTG CCGAGGGCAC 601 CGCTGTCCAA GTTCTGGCCA GANTACTCGA TCATCNACTT GGTGTTGGAA CCGCTNACGG 661 TCCAAGTGNT GGNCTTGCTN CCCCNTGGGN AGTCCNNGTA NANNNGGNNN ANNNGCTNNN 721 CGNNNTCGGC GGGNNNNNNN NNN // |
Primer: pGL3_RV4 (Pr0335) Region: lacI 3' Sequence file: RDB13962_A6Btg.seq ![]() |
>D03144A5_A6Bt_1_pGL3_RV4_G12_21.ab1 1 NNNNNNNNNN NNNNNNGNTC TCAGGGCNTC GGTCGANNNC CCGGTGCCTA ATGAGTGAGC 61 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC 121 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG 181 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC 241 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT 301 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC 361 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC 421 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT 481 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT 541 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT 601 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGANCCAAT GCGACCAGAT GCTCCACGCC 661 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGANAC 721 ATCAAGAAAT AACGCCNGAA CATTAGTGCA NGCAGCTTCC ACAGCAATGG CATCCTGGTC 781 ATCCAGCGGA TAGTTAATGA TCAGCCCANN GANGCGTTGC GCGAGAAGNN TGTGCANCGC 841 CGCTTTNCAG GCNTCNACGC CGCTTCNTTC TANCNTCNAC NCCACCACNC NNNNACCCAG 901 TTGNNNNNNG NNANATTTNA TCGNNNNNAN NNNTTGCNAN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215. [link to RRC of NBRP] |
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original | Bhat, H.B., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047. [link to RRC of NBRP] |
protocol | Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303. |
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