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pET28/PlyA2-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.

Catalog number RDB13962
Resource name pET28/PlyA2-EGFP-His6
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.
Expression was confirmed by the depositor.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Km^r
Gene/insert name Pleurotus eryngii PlyA2 cDNA Aequorea victoria GFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., J Lipid Res. 54 (10): 2933-2943, 2013.)
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Schedule A (GFP-A) [Link]
Remarks
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RDB13962 pET28/PlyA2-EGFP-His6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303.
original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.
original Bhat, H.B., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13962_A6Btp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR 322 BamHI R RDB13962_A6Bta.seq
>D03144A5_A6Bt_1_pBR 322 BamHI R_D04_10.ab1
    1 TTTGCACATA TACGACTCAC TATAGGGGAA TTGTGAGCGG ATAACAATTC CCCTCTAGAA
   61 ATAATTTTGT TTAACTTTAA GAAGGAGATA TACCGGTACC ATGGCATACG CACAATGGGT
  121 CATCATCATC ATCCACAACG TCGGGTCCAA AGACGTGAAG ATCGTAAACC TCAAGCCTTC
  181 TTGGGGGAAG CTTCATGCCG ACGGCGACAA AGACACAGAA GTCTCAGCAA GCAAGTACGA
  241 AGGAACAGTC ATCAAGCCTG ACGAGAAGCT CCAGATCAAT GCCTGCGGCA GATCAGACGC
  301 CGCCGAAGGC ACGACAGGAA CCTTCGACCT TGTTGACCCC GCCGATGGCG ACAAGCAGGT
  361 CCGCCACTTC TACTGGGACT GCCCATGGGG AAGCAAGGCC AACACTTGGA CCGTTAGCGG
  421 TTCCAACACC AAGTGGATGA TCGAGTACTC TGGCCAGAAC TTGGACAGCG GTGCCCTCGG
  481 CACGATCACC GTCGACACCT TGAAGAAGGG GAACGAATTC GGAGGAGGAG GAGGAGGATC
  541 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
  601 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
  661 TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
  721 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
  781 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
  841 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
  901 GGTGAACCGC ATCGAGCTGA AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC
  961 ACAAGCTGGA GTACAACTAC ACAGCCACAA CGTCTATATC ATGGCCGACA GCAGAAGACG
 1021 GCATCAAGGT GACTTCAGAT CGCCACACAT CGAGACGCCA GCGTGCAGCT CGGCGGACAC
1081 TACCAGCAGA ACACCTCATT CGCGACCGGA CTCGGGTGCC TGTGCCTGGT CA
//
Primer: T7Terminator RDB13962_A6Btb.seq
>D03144A5_A6Bt_1_T7Terminator_C04_07.ab1
    1 CAAAGCTTCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGAAGC
   61 TTCTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG
  121 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG
  181 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
  241 GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG
  301 TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG
  361 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
  421 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
  481 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
  541 TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG
  601 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCA
  661 TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  721 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  781 GATCCTCCTC CTCCTCCTCC GAATTCGTTC CCCTTCTTCA AGGTGTCGAC GGTGATCGTG
  841 CCGAGGGCAC CGCTGTCCAA GTTCTGGCCC AGAGTACTCG ATCATCCACT TTGGTGTTGG
  901 GAACCCGCTA ACGGTCCAAG TGTTTGGCCT TGCTTCCCCA TGGGCAGTCC CCAGTAGAAA
  961 GTGGCGGAAC CTGCTTGTCG CCATCGGGCG GGGTCAACAA GGTCGAACGG TTTCCTGTCG
 1021 TGCCTTCGCC GGCCGTCTGA ATCTGCCGCA GGCCATTGAA TCCTGGAAGC TTTCTCGGTC
 1081 AGCTTGGAAT GACCTGGATT CCTCCGTTAC CTTGCCTTGC TTGAGACCTT CCTGTGGTCC
1141 TTTTGGTTCC GA
//
Primer: pGL3_RV4 RDB13962_A6Btc.seq
>D03144A5_A6Bt_1_pGL3_RV4_G12_21.ab1
    1 GAAACTCTGG GTTGAGGCTC TCAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC
   61 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC
  121 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG
  181 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC
  241 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT
  301 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC
  361 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC
  421 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT
  481 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT
  541 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT
  601 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC
  661 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC
  721 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC
  781 ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC
  841 CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG
  901 TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCCAGAC
  961 TGGATGTGGG CAACGCCATC AGCAACGACT GTTTGCCGCA GTGTTGTGCC ACGCGGATGG
 1021 ATGTATCCAG CCTCCGCATC GCCGCTCAAC TTTTCCCGCG TTCGCAGGAT CGTGCCTTGC
1081 TTGATCAACA CGGCCGGGTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Abe, M., Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis. Methods Cell Biol. 137: 15-24 (2017). PMID 28065303.
original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.
original Bhat, H.B., Binding of a pleurotolysin ortholog from Pleurotus eryngii to sphingomyelin and cholesterol-rich membrane domains. J. Lipid Res. 54 (10): 2933-2943 (2013). PMID 23918047.

2019.06.21

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