Sequencing and PCR primers for primer information.
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
| Catalog number | RDB13959 |
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| Resource name | pQE30/His6-Venus-NT-Lys |
| Clone info. | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin. Expression was confirmed by the depositor. |
| Vector backbone | pQE-30 (Plasmid) |
| Size of vector backbone | 3.5 kb |
| Selectable markers | Amp^r |
| Gene/insert name | Eisenia foetida lysenin cDNA Aequorea victoria GFP cDNA |
| Depositor | Kobayashi, Toshihide | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.) |
|---|---|
| Additional terms and conditions for distribution | RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Approval form [Word] by Dr. Atsushi Miyawaki |
| Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13959 | pQE30/His6-Venus-NT-Lys | DNA solution |
Electronic file
Featured content
| Featured content | Sphingolipid signaling pathway (English text) |
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| Featured content | Organelle marker (English text) |
References
| original | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13959_A6Btp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pJB8_Forward | RDB13959_A6Bta.seq |
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>D03144A2_A6Bt_1_pJB8_Forward_C12_09.ab1 1 CTCAACTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA 61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCACTAGT AACGGCCGCC 121 AGTGTGCTGG AATTCGCCCT TCCGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG 181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 241 CCGGTGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA 301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCATGCAGT 361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC GAGACCCGCG 481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG 601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA 661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG 721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCAAA CTGAGCAAAG 781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA 841 CTCTCGGCAT GGACGAGCTG TACAAGATAA TCCTTGGAAA AACAGAAATT CGAATTAAGC 901 ATGCAGAAAG GAAGGAGTAC ATGACAGTCG TTTCAAGAAA AAGTTGGCCA GCTGCAACTC 961 TTGGGACATA GCAACTTTTC AAGTTTGTGC TCTATGAAGA TTGGGGGGGG GGATTTCGAA 1021 ATTTAAAACG CTGACACCAA TGTATTCGGG CTATGAGTAT GCCCTAATCC TCTGAATCAC 1081 GAAGGAGTCT ACCTTGGATC AGTACTGGAT ATTCCGAAAA CAGGCCGCCT GGGGCCAAA // |
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| Primer: Cm_N_rev | RDB13959_A6Btb.seq |
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>D03144A2_A6Bt_1_Cm_N_rev_D12_12.ab1 1 CGAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT 61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT 121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG 181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC 241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC 301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC 361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG 421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT 481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT 541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT 601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTTGT ACAGCTCGTC 661 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT 721 CTCGTTGGGG TCTTTGCTCA GTTTGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG 781 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC 841 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC 901 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC 961 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA 1021 ACTTCACCCT CGGCGCGGGT CTCGTAGTTG CCGTCGTCTT TGAAGAAGAT GGTTGCGCTT 1081 CTTGGACGTT AGCATTCCGG TCAATGGACG GGACTTGAAA GAAAAGATCG GATG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. |
|---|
2018.11.09
GNP_filter3_RDBDEP_html_181012.pl