Resource data sheet
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pQE30/His6-Venus-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.

Catalog number RDB13959
Resource name pQE30/His6-Venus-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
Expression was confirmed by the depositor.
Vector backbone pQE-30 (Plasmid)
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA Aequorea victoria GFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)
Additional terms and conditions for distribution RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
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Approval form [Word] by Dr. Atsushi Miyawaki
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13959 pQE30/His6-Venus-NT-Lys DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

References

original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13959_A6Btp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pJB8_Forward RDB13959_A6Bta.seq
>D03144A2_A6Bt_1_pJB8_Forward_C12_09.ab1
    1 CTCAACTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA
   61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCACTAGT AACGGCCGCC
  121 AGTGTGCTGG AATTCGCCCT TCCGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  241 CCGGTGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA
  301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCATGCAGT
  361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC GAGACCCGCG
  481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
  601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA
  661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
  721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCAAA CTGAGCAAAG
  781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
  841 CTCTCGGCAT GGACGAGCTG TACAAGATAA TCCTTGGAAA AACAGAAATT CGAATTAAGC
  901 ATGCAGAAAG GAAGGAGTAC ATGACAGTCG TTTCAAGAAA AAGTTGGCCA GCTGCAACTC
  961 TTGGGACATA GCAACTTTTC AAGTTTGTGC TCTATGAAGA TTGGGGGGGG GGATTTCGAA
 1021 ATTTAAAACG CTGACACCAA TGTATTCGGG CTATGAGTAT GCCCTAATCC TCTGAATCAC
1081 GAAGGAGTCT ACCTTGGATC AGTACTGGAT ATTCCGAAAA CAGGCCGCCT GGGGCCAAA
//
Primer: Cm_N_rev RDB13959_A6Btb.seq
>D03144A2_A6Bt_1_Cm_N_rev_D12_12.ab1
    1 CGAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
   61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT
  121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
  181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC
  241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC
  301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC
  361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG
  421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT
  481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT
  541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT
  601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTTGT ACAGCTCGTC
  661 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
  721 CTCGTTGGGG TCTTTGCTCA GTTTGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
  781 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC
  841 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC
  901 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
  961 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
 1021 ACTTCACCCT CGGCGCGGGT CTCGTAGTTG CCGTCGTCTT TGAAGAAGAT GGTTGCGCTT
1081 CTTGGACGTT AGCATTCCGG TCAATGGACG GGACTTGAAA GAAAAGATCG GATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.

2018.11.09

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