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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pQE30/His6-Venus-NT-Lys (#RDB13959)

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.

Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
Comment Expression was confirmed by the depositor.
Vector backbone pQE-30 (Plasmid)
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37oC
Gene/insert name Eisenia foetida lysenin cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)
Additional terms and conditions:
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)。

付加的使用条件:
本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13959 pQE30/His6-Venus-NT-Lys DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pQE30/His6-Venus-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13959).

Reference section:

Kiyokawa, E., Baba, T., Otsuka, N., Makino, A., Ohno, S., Kobayashi, T., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13959_A6Btp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pJB8_Forward
Sequence file: RDB13959_A6Bta.seq check
>D03144A2_A6Bt_1_pJB8_Forward_C12_09.ab1
    1 CTCAACTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA
   61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCACTAGT AACGGCCGCC
  121 AGTGTGCTGG AATTCGCCCT TCCGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  241 CCGGTGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA
  301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCATGCAGT
  361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC GAGACCCGCG
  481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
  601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA
  661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
  721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCAAA CTGAGCAAAG
  781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
  841 CTCTCGGCAT GGACGAGCTG TACAAGATAA TCCTTGGAAA AACAGAAATT CGAATTAAGC
  901 ATGCAGAAAG GAAGGAGTAC ATGACAGTCG TTTCAAGAAA AAGTTGGCCA GCTGCAACTC
  961 TTGGGACATA GCAACTTTTC AAGTTTGTGC TCTATGAAGA TTGGGGGGGG GGATTTCGAA
 1021 ATTTAAAACG CTGACACCAA TGTATTCGGG CTATGAGTAT GCCCTAATCC TCTGAATCAC
1081 GAAGGAGTCT ACCTTGGATC AGTACTGGAT ATTCCGAAAA CAGGCCGCCT GGGGCCAAA
//
Primer: Cm_N_rev
Sequence file: RDB13959_A6Btb.seq check
>D03144A2_A6Bt_1_Cm_N_rev_D12_12.ab1
    1 CGAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
   61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT
  121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
  181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC
  241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC
  301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC
  361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG
  421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT
  481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT
  541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT
  601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTTGT ACAGCTCGTC
  661 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
  721 CTCGTTGGGG TCTTTGCTCA GTTTGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
  781 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC
  841 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC
  901 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
  961 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
 1021 ACTTCACCCT CGGCGCGGGT CTCGTAGTTG CCGTCGTCTT TGAAGAAGAT GGTTGCGCTT
1081 CTTGGACGTT AGCATTCCGG TCAATGGACG GGACTTGAAA GAAAAGATCG GATG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. [link to RRC of NBRP]