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by Dr. Atsushi MiyawakiPlease obtain written approval of Dr. Atsushi Miyawaki.
icon below as well as clone information before placing your order.
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
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Other clones in our bank
External Database Eisenia foetida lysenin Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.) |
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| Additional terms and conditions for distribution | RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS regarding Venus. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Approval form [Word] by Dr. Atsushi Miyawaki |
| Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)。 |
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| 付加的提供条件 | 利用者は、Venusについて理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。非営利機関の非営利学術研究に限って提供する。 |
| 提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]宮脇敦史博士による提供承諾書 [Word ] |
| 備考 | 宮脇敦史博士の書面による提供承諾が必要です。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB13959 | pQE30/His6-Venus-NT-Lys | DNA solution |
icon below as well as clone information before placing your order.Electronic file
Original reference
| original | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Visualization of Organelles (English text) |
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| Featured content | Sphingolipid signaling pathway (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB13959_A6Btp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pJB8_Forward Sequence file: RDB13959_A6Bta.seq
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>D03144A2_A6Bt_1_pJB8_Forward_C12_09.ab1 1 CTCAACTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA 61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCACTAGT AACGGCCGCC 121 AGTGTGCTGG AATTCGCCCT TCCGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG 181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 241 CCGGTGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA 301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCATGCAGT 361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC GAGACCCGCG 481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG 601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA 661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG 721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCAAA CTGAGCAAAG 781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA 841 CTCTCGGCAT GGACGAGCTG TACAAGATAA TCCTTGGAAA AACAGAAATT CGAATTAAGC 901 ATGCAGAAAG GAAGGAGTAC ATGACAGTCG TTTCAAGAAA AAGTTGGCCA GCTGCAACTC 961 TTGGGACATA GCAACTTTTC AAGTTTGTGC TCTATGAAGA TTGGGGGGGG GGATTTCGAA 1021 ATTTAAAACG CTGACACCAA TGTATTCGGG CTATGAGTAT GCCCTAATCC TCTGAATCAC 1081 GAAGGAGTCT ACCTTGGATC AGTACTGGAT ATTCCGAAAA CAGGCCGCCT GGGGCCAAA // |
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Primer: Cm_N_rev Sequence file: RDB13959_A6Btb.seq
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>D03144A2_A6Bt_1_Cm_N_rev_D12_12.ab1 1 CGAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT 61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT 121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG 181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC 241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC 301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC 361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG 421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT 481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT 541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT 601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTTGT ACAGCTCGTC 661 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT 721 CTCGTTGGGG TCTTTGCTCA GTTTGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG 781 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC 841 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC 901 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC 961 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA 1021 ACTTCACCCT CGGCGCGGGT CTCGTAGTTG CCGTCGTCTT TGAAGAAGAT GGTTGCGCTT 1081 CTTGGACGTT AGCATTCCGG TCAATGGACG GGACTTGAAA GAAAAGATCG GATG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. |
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2022.05.17
GNP_filter3_RDBDEP_html_220516.pl