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pQE30/His6-mRFP-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.

Catalog number RDB13960
Resource name pQE30/His6-mRFP-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.
Expression was confirmed by the depositor.
Vector backbone pQE-30 (Plasmid)
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA Discosoma sp. RFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)
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RDB13960 pQE30/His6-mRFP-NT-Lys DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

References

original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13960_A6Btp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pJB8_Forward RDB13960_A6Bta.seq
>D03144A3_A6Bt_1_pJB8_Forward_E12_15.ab1
    1 TTATTTGCTT TGTGAGCGGA TAACAATTTC ACACAGAATT CATTAAAGAG GAGAAATTAA
   61 CTATGAGAGG ATCGCATCAC CATCACCATC ACGGATCCAT GGCCTCCTCC GAGGACGTCA
  121 TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG
  181 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG
  241 TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCCAGTACG
  301 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC
  361 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA
  421 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA
  481 ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG
  541 AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGATGAGG CTGAAGCTGA
  601 AGGACGGCGG CCACTACGAC GCCGAGGTCA AGACCACCTA CATGGCCAAG AAGCCCGTGC
  661 AGCTGCCCGG CGCCTACAAG ACCGACATCA AAGCTGGACA TCACCTCCCA CAACGAGGAC
  721 TACACCATCG TGGAACAGTA CGAGCGCGCC GAGGGCCCGC CCACTCCCAC CCGGCGCCGT
  781 CGAGATAATC CTTTGGAAAA ACAAAAATTC CAAATTAAGC CATGCAGAAA AGAAAGGAGT
841 AACTTGACAG T
//
Primer: Cm_N_rev RDB13960_A6Btb.seq
>D03144A3_A6Bt_1_Cm_N_rev_F12_18.ab1
    1 CAAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
   61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT
  121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
  181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC
  241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC
  301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC
  361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG
  421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT
  481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT
  541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT
  601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTCGA CGGCGCCGGT
  661 GGAGTGGCGG CCCTCGGCGC GCTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA
  721 GGTGATGTCC AGCTTGATGT CGGTCTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC
  781 CATGTAGGTG GTCTTGACCT CGGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCAT
  841 CTTGATCTCG CCCTTCAGGG CGCCGTCCTC GGGGTACATC CGCTCGGTGG AGGCCTCCCA
  901 GCCCATGGTC TTCTTCTGCA TTACAGGGCC GTCGGAGGGA AGTTGGTGCC GCGCAGCTTC
  961 ACCTTGTAGA TGAACTCGCC GTCCTGCAGG GAGGAGTCCT GGGTCACGGT CACCACGCCG
 1021 CCGTCCTCGA AGTCATCACG CGCTCCACTT GAGGCCTCGG GGAGACAGCT CAAGTAGTCC
1081 GGATGTCGCA GGGTGGCCTT CACGTAAGGA CCCATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.

2018.12.10

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