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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pQE30/His6-mRFP-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.

Catalog number RDB13960
Resource name pQE30/His6-mRFP-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.
Comment Expression was confirmed by the depositor.
Vector backbone pQE-30 (Plasmid)
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA Discosoma sp. RFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
Eisenia foetida lysenin
    Discosoma sp. RFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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            RDB13960 pQE30/His6-mRFP-NT-Lys DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13960_A6Btp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pJB8_Forward
            Sequence file: RDB13960_A6Bta.seq check
            >D03144A3_A6Bt_1_pJB8_Forward_E12_15.ab1
                1 TTATTTGCTT TGTGAGCGGA TAACAATTTC ACACAGAATT CATTAAAGAG GAGAAATTAA
               61 CTATGAGAGG ATCGCATCAC CATCACCATC ACGGATCCAT GGCCTCCTCC GAGGACGTCA
              121 TCAAGGAGTT CATGCGCTTC AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG
              181 AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG
              241 TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCCAGTACG
              301 GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC
              361 CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA
              421 CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA
              481 ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG
              541 AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGATGAGG CTGAAGCTGA
              601 AGGACGGCGG CCACTACGAC GCCGAGGTCA AGACCACCTA CATGGCCAAG AAGCCCGTGC
              661 AGCTGCCCGG CGCCTACAAG ACCGACATCA AAGCTGGACA TCACCTCCCA CAACGAGGAC
              721 TACACCATCG TGGAACAGTA CGAGCGCGCC GAGGGCCCGC CCACTCCCAC CCGGCGCCGT
              781 CGAGATAATC CTTTGGAAAA ACAAAAATTC CAAATTAAGC CATGCAGAAA AGAAAGGAGT
            841 AACTTGACAG T
            //
            Primer: Cm_N_rev
            Sequence file: RDB13960_A6Btb.seq check
            >D03144A3_A6Bt_1_Cm_N_rev_F12_18.ab1
                1 CAAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
               61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT
              121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
              181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC
              241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC
              301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC
              361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG
              421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT
              481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT
              541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT
              601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTCGA CGGCGCCGGT
              661 GGAGTGGCGG CCCTCGGCGC GCTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA
              721 GGTGATGTCC AGCTTGATGT CGGTCTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC
              781 CATGTAGGTG GTCTTGACCT CGGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCAT
              841 CTTGATCTCG CCCTTCAGGG CGCCGTCCTC GGGGTACATC CGCTCGGTGG AGGCCTCCCA
              901 GCCCATGGTC TTCTTCTGCA TTACAGGGCC GTCGGAGGGA AGTTGGTGCC GCGCAGCTTC
              961 ACCTTGTAGA TGAACTCGCC GTCCTGCAGG GAGGAGTCCT GGGTCACGGT CACCACGCCG
             1021 CCGTCCTCGA AGTCATCACG CGCTCCACTT GAGGCCTCGG GGAGACAGCT CAAGTAGTCC
            1081 GGATGTCGCA GGGTGGCCTT CACGTAAGGA CCCATG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl