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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pQE30/His6-mRFP-NT-Lys (#RDB13960)

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.

Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.
Comment Expression was confirmed by the depositor.
Vector backbone pQE-30 (Plasmid)
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA Discosoma sp. RFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13960 pQE30/His6-mRFP-NT-Lys DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pQE30/His6-mRFP-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13960).

Reference section:

Kiyokawa, E., Baba, T., Otsuka, N., Makino, A., Ohno, S., Kobayashi, T., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13960_A6Kgp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pJB8-Forward (Pr0084)
Region: 6xHis,insert 5'(mRFP1 5')
Sequence file: RDB13960_A6Kgc.seq check
>13960_13960_A6Kg_pJB8-Forward_C10_07_ABI24.ab1
    1 NNNNNNNNTN NTTTGNTTTG TGAGCGGNNA ACNATTTNNC NCAGAATTCA TTAAAGAGGA
   61 GAANTTAACT ATGAGAGGAT CGCATCACCA TCACCATCAC GGATCCATGG CCTCCTCCGA
  121 GGACGTCATC AAGGAGTTCA TGCGCTTCAA GGTGCGCATG GAGGGCTCCG TGAACGGCCA
  181 CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCACCC AGACCGCCAA
  241 GCTGAAGGTG ACCAAGGGCG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCTCAGTT
  301 CCAGTACGGC TCCAAGGCCT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACTTGAAGCT
  361 GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGT
  421 GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCGAGTTC ATCTACAAGG TGAAGCTGCG
  481 CGGCACCAAC TTCCCCTCCG ACGGCCCCGT AATGCAGAAG AAGACCATGG GCTGGGAGGC
  541 CTCCACCGAG CGGATGTACC CCGAGGACGG CGCCCTGAAG GGCGAGATCA AGATGAGGCT
  601 GAAGCTGAAG GACGGCGGCC ACTACGACGC CGAGGTCAAG ACCACCTACA TGGCCAAGAA
  661 GCCCGTGCAG CTGCCCGGCG CCTACAAGAC CGACATCAAG CTGGACATCA CCTCCCACAA
  721 CGAGGACTAC ACCATCGTGN AACAGTACGA GCGCGCCGAN GGCCGCCACT CCNCCGGCGC
  781 CGTCNAGATA ATCCTTGGAA AAACAGAAAT TCGAATTAAG CATGCAGAAA GGAANGANTA
  841 CATGACAGTC GTTTCNAGAA AAANTTGGNN NGCTGCAACT CTNNNANNTA GCAAACTTTT
  901 CNAGTTTGNG NTCNATGNAN ANNNNGGGGG GGGNNTTCNA ATTNAAACGC TNNNNACCNN
  961 GTANTCNGNC TNTNANTNTG NNNNNTNNNN NNNTNNNNNN NGNNNNNNNN TNNNNNNNNN
1021 N
//
Primer: Cm_N_rev (Pr0014)
Region: lamda t0 ter,insert 3'(NT-Lys,mRFP 3')
Sequence file: RDB13960_A6Kgd.seq check
>13960_13960_A6Kg_Cm_N_rev_D10_10_ABI24.ab1
    1 NNNNNNNNNN NGTTCTTNNN GATGCNNTTG GGNNATATNA ACGGTGGTAT ATCCNGTGAT
   61 TTTTTTCTCC NTTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT
  121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
  181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC
  241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC
  301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC
  361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG
  421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT
  481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT
  541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT
  601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTCGA CGGCGCCGGT
  661 GGAGTGGCGG CCCTCGGCGC GCTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA
  721 GGTGATGTCC AGCTTGATGT CGGTCTTGTA NGCGCCGGGC AGCTGCACGG GCTTCTTGGC
  781 CATGTANGTG GTCTTGANCT CGGCGTCGTA GTGGCCGCCG TCCTTCANCT TCNGCCTCAT
  841 CTTGATCTCG CCCTTCNNGG CGCCNTCCTC GGGGNACATC CGCTCGGNGG ANGNCTCCCN
  901 GCCCANNGNC TTCTTCTGNA NNACGGGGNC GTCNNNNGGG AANNNGGNGN CGCGCANCTT
961 CNNCTTNNAN ATGNNNNCNN NGNNNNGNNN GNANNANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. [link to RRC of NBRP]
user_report Miyata, Y., Protocol to analyze lipid asymmetry in the plasma membrane. STAR Protoc. 3 (4): 101870 (2022). PMID 36595929. [link to RRC of NBRP]