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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pTRE-SaSMaseC 

Mammalian expression vector of Staphylococcus aureus sphingomyelinase C (SMase C), Tet-On/Off Advanced IRES mCherry system.

Catalog number RDB19219
Resource name pTRE-SaSMaseC 
Clone info. Mammalian expression vector of Staphylococcus aureus sphingomyelinase C (SMase C), Tet-On/Off Advanced IRES mCherry system.
Comment Expression was confirmed by the depositor with microscopy.
Vector backbone pTRE-Dual2 (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Ampicillin
Gene/insert name Staphylococcus aureus beta-toxin cDNA
Depositor|Developer Abe, Mitsuhiro |
Other clones in our bank

External Database
Staphylococcus aureus beta-toxin

          Reference sequence
            
           
          Sequence (full) RDB19219zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19219 pTRE-SaSMaseC  DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021).

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19219_B1Grp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: IRES-F
          Region: IRES2, insert 5'
          Sequence file: RDB19219_B1Gra.seq check
          >D08145B8_B1Gr_2_IRES-F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNNG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
             61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCGCGGC CGCTAGATCT
            121 GGATCCATGG TTAAGAAAAC TAAAAGCAAT AGCCTGAAAA AAGTGGCGAC ATTAGCATTG
            181 GCCAATCTGT TGCTGGTTGG TGCCTTAACC GATAACTCCA GCAAAGCCGA AAGCAAGAAG
            241 GATGACACTG ATCTCAAACT GGTAAGCCAC AATGTGTACA TGTTGTCTAC CGTACTGTAT
            301 CCCAATTGGG GACAGTACAA ACGTGCAGAT CTGATTGGTC AGTCGAGCTA CATCAAGAAT
            361 AACGATGTCG TCATCTTCAA TGAAGCGTTT GACAATGGGG CGTCTGATAA ACTGCTTAGT
            421 AACGTTAAGA AAGAGTATCC CTATCAGACC CCTGTCTTAG GTCGCTCACA ATCTGGCTGG
            481 GACAAAACGG AAGGCTCGTA TAGTTCGACC GTTGCTGAGG ATGGTGGCGT TGCGATTGTC
            541 TCCAAATATC CGATCAAAGA GAAAATTCAG CACGTGTTCA AGTCCGGTTG TGGCTTTGAT
            601 AACGATAGTA ACAAAGGCTT TGTGTATACC AAAATTGAGA AGAATGGCAA AAACGTCCAT
            661 GTTATTGGAA CGCACACTCA GTCAGAAGAT AGTCGTTGCG GTGCTGGCCA TGACCGCAAA
            721 ATTCGCGCTG AACAGATGAA AGAAATCTCC GANTTCGTAA AGAAAAAAAA TATTCCGAAA
            781 GANGAAANCG TGTATATTGG TGGGGATCTG AACGTGAACA AAGGGACGCC AGAATTTNAA
            841 GANNTGCTGA NGAANCNTGA NCGTGAATGA TGTACTTTAC GCGGGCCATN NCTCNNCATG
            901 GGNNCCGCAN NCTNNNAGCA TCGCNAAATA CNNCTATNCG AANNNNNNNN NAGANNNNCN
          961 NGGANTACAT NTTTNNNNNN ANNNNNNNNA NNNANNNNNN NNNN
          //
          Primer: SV40polyA_R2
          Region: insert 3'
          Sequence file: RDB19219_B1Grb.seq check
          >D08145B8_B1Gr_2_SV40polyA_R2_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA CNAGTTAACN ANNACNATTG CATTCNTTTT ATGTTTCAGG
             61 TTCAGGGGGN NGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
            121 CTGATTATGA TCCTCTAGAC TGCAGTTATT TCGAATAGGC TTTAATTGGG TAATGATCGC
            181 TGAAGTCGTT ATACACATAG TAGTACGGAA ATGCATAAAC GTCCCAAGGT TTCGGTTTCT
            241 CGGTCACGAC TTCGTTCACG AGTTGCTTCG GTTGTTTATG ATCTTTATCC GTAAAGATGT
            301 AATCCAGGTG TTCTGGTTTG CCATTCGGAT AGTTGTATTT CGCGATGCTA TTAGACTGCG
            361 GGTCCCATGT TGAGTTATGG CCCGCGTAAA GTACATCATT CACGTTCAGG TTCTTCAGCA
            421 TGTCTTTAAA TTCTGGCGTC CCTTTGTTCA CGTTCAGATC CCCACCAATA TACACGGTTT
            481 CGTCTTTCGG AATATTTTTT TTCTTTACGA AGTCGGAGAT TTCTTTCATC TGTTCAGCGC
            541 GAATTTTGCG GTCATGGCCA GCACCGCAAC GACTATCTTC TGACTGAGTG TGCGTTCCAA
            601 TAACATGGAC GTTTTTGCCA TTCTTCTCAA TTTTGGTATA CACAAAGCCT TTGTTACTAT
            661 CGTTATCAAA GCCACAACCG GACTTGAACA CGTGCTGAAT TTTCTCTTTG ATCGGATATT
            721 TGGAGACAAT CGCAACGCCA CCATCCTCAG CAACGGTCGA ACTATACGAG CCTTCCGTTT
            781 TGTCCCAGCC AGATTGTGAG CGANCTAAGA NAGGGGTCTG ATAGGGATAC TCTTTCTTAA
            841 CGTTACNNNN CAGTTTATCA NANGCCCCAT TGTCAAACGC TTCNTNNNNN ATGANNANAT
            901 CNNTNNTTCT TGATGTANCT CNANNNNNNN NNTCANANCT GCACNNTNGN ACNNNCCCNN
          961 NNTNGGGANN NNNNNNNGNN ANNNNNNNNN N
          //
          Primer: IRES-R2
          Region: IRES2, mCherry 3'
          Sequence file: RDB19219_B1Grc.seq check
          >D08145B8_B1Gr_2_IRES-R2_C01_07_ABI24.ab1
              1 GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGANAGGGG
             61 CGCTAGCCTA CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT
            121 CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC TTGATGTTGA
            181 CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCAG
            241 CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC
            301 CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA
            361 CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT
            421 CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC
            481 GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT
            541 GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG
            601 GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC
            661 CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGTGCACCT
            721 TGAAGCGCAT GAACTCCTTG ATGATGNCCA TGTTATCCTC CTCGCCCTTG CTCACCATGG
            781 TGGCGAATTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTNAT ATAGGCCTCC
            841 CACCGTACAC GCCTACCTCG ACATACGTTC TCTATCNCNG ATAGGGNNTA AACTCGACAT
            901 ACNTTCTCTA TCACNGATAG GGATAAACTC NACNTACNNT CNCTATCANN NNNAGGGNNN
          961 NANNNCNNNN TACNNTNNTC NNNNNNNNNN
          //
          Primer: mRFP-M_R
          Region: mCherry 5', tight TRE pro
          Sequence file: RDB19219_B1Grd.seq check
          >D08145B8_B1Gr_2_mRFP-M_R_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGCCTNNGNN CCGTNNATGN ACTGNNGGGN NAGGNNGTCC
             61 CAGGCGAAGG GCAGGNGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG
            121 TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC
            181 ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC CTCGCCCTTG
            241 CTCACCATGG TGGCGAATTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT
            301 ATAGGCCTCC CACCGTACAC GCCTACCTCG ACATACGTTC TCTATCACTG ATAGGGAGTA
            361 AACTCGACAT ACGTTCTCTA TCACTGATAG GGATAAACTC GACATACGTT CTCTATCACT
            421 GATAGGGAGT AAACTCGACA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG
            481 TTCTCTATCA CTGATAGGGA GTAAACTCGA CATCGTTCTC TATCACTGAT AGGGAGTAAA
            541 CTCGACATAC GTTCTCTATC ACTGATAGGG AGTAAACTCG AGTGAAGACG AAAGGGCCTC
            601 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA NGGTTTCTTA GACGTCAGGT
            661 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA
            721 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG
            781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTNG CGGCATTTTG
            841 CCTTCCTGNT TTTGCTCACC CANAAACGCT GGNGAAAGTN AAAGANGCTG AAGATCAGTT
            901 GGGNGNNNGA NNGGGTTACA TCNAACTNGN TCTCNNNNGC NNNNAGANNC NNNANANTTT
          961 TCNNCCNNNN NNNNNTTNNC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021).

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl