Please review the QC test results indicated by icon below as well as clone information before placing your order.

pTRE-SaSMaseC 

Mammalian expression vector of Staphylococcus aureus sphingomyelinase C (SMase C), Tet-On/Off Advanced IRES mCherry system.

Sequence (full)	RDB19219zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19219_B1Grp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-F (Pr0036) Region: IRES2, insert 5' Sequence file: RDB19219_B1Gra.seq
>D08145B8_B1Gr_2_IRES-F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCGCGGC CGCTAGATCT   121 GGATCCATGG TTAAGAAAAC TAAAAGCAAT AGCCTGAAAA AAGTGGCGAC ATTAGCATTG   181 GCCAATCTGT TGCTGGTTGG TGCCTTAACC GATAACTCCA GCAAAGCCGA AAGCAAGAAG   241 GATGACACTG ATCTCAAACT GGTAAGCCAC AATGTGTACA TGTTGTCTAC CGTACTGTAT   301 CCCAATTGGG GACAGTACAA ACGTGCAGAT CTGATTGGTC AGTCGAGCTA CATCAAGAAT   361 AACGATGTCG TCATCTTCAA TGAAGCGTTT GACAATGGGG CGTCTGATAA ACTGCTTAGT   421 AACGTTAAGA AAGAGTATCC CTATCAGACC CCTGTCTTAG GTCGCTCACA ATCTGGCTGG   481 GACAAAACGG AAGGCTCGTA TAGTTCGACC GTTGCTGAGG ATGGTGGCGT TGCGATTGTC   541 TCCAAATATC CGATCAAAGA GAAAATTCAG CACGTGTTCA AGTCCGGTTG TGGCTTTGAT   601 AACGATAGTA ACAAAGGCTT TGTGTATACC AAAATTGAGA AGAATGGCAA AAACGTCCAT   661 GTTATTGGAA CGCACACTCA GTCAGAAGAT AGTCGTTGCG GTGCTGGCCA TGACCGCAAA   721 ATTCGCGCTG AACAGATGAA AGAAATCTCC GANTTCGTAA AGAAAAAAAA TATTCCGAAA   781 GANGAAANCG TGTATATTGG TGGGGATCTG AACGTGAACA AAGGGACGCC AGAATTTNAA   841 GANNTGCTGA NGAANCNTGA NCGTGAATGA TGTACTTTAC GCGGGCCATN NCTCNNCATG   901 GGNNCCGCAN NCTNNNAGCA TCGCNAAATA CNNCTATNCG AANNNNNNNN NAGANNNNCN 961 NGGANTACAT NTTTNNNNNN ANNNNNNNNA NNNANNNNNN NNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB19219_B1Grb.seq
>D08145B8_B1Gr_2_SV40polyA_R2_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNA CNAGTTAACN ANNACNATTG CATTCNTTTT ATGTTTCAGG    61 TTCAGGGGGN NGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG   121 CTGATTATGA TCCTCTAGAC TGCAGTTATT TCGAATAGGC TTTAATTGGG TAATGATCGC   181 TGAAGTCGTT ATACACATAG TAGTACGGAA ATGCATAAAC GTCCCAAGGT TTCGGTTTCT   241 CGGTCACGAC TTCGTTCACG AGTTGCTTCG GTTGTTTATG ATCTTTATCC GTAAAGATGT   301 AATCCAGGTG TTCTGGTTTG CCATTCGGAT AGTTGTATTT CGCGATGCTA TTAGACTGCG   361 GGTCCCATGT TGAGTTATGG CCCGCGTAAA GTACATCATT CACGTTCAGG TTCTTCAGCA   421 TGTCTTTAAA TTCTGGCGTC CCTTTGTTCA CGTTCAGATC CCCACCAATA TACACGGTTT   481 CGTCTTTCGG AATATTTTTT TTCTTTACGA AGTCGGAGAT TTCTTTCATC TGTTCAGCGC   541 GAATTTTGCG GTCATGGCCA GCACCGCAAC GACTATCTTC TGACTGAGTG TGCGTTCCAA   601 TAACATGGAC GTTTTTGCCA TTCTTCTCAA TTTTGGTATA CACAAAGCCT TTGTTACTAT   661 CGTTATCAAA GCCACAACCG GACTTGAACA CGTGCTGAAT TTTCTCTTTG ATCGGATATT   721 TGGAGACAAT CGCAACGCCA CCATCCTCAG CAACGGTCGA ACTATACGAG CCTTCCGTTT   781 TGTCCCAGCC AGATTGTGAG CGANCTAAGA NAGGGGTCTG ATAGGGATAC TCTTTCTTAA   841 CGTTACNNNN CAGTTTATCA NANGCCCCAT TGTCAAACGC TTCNTNNNNN ATGANNANAT   901 CNNTNNTTCT TGATGTANCT CNANNNNNNN NNTCANANCT GCACNNTNGN ACNNNCCCNN 961 NNTNGGGANN NNNNNNNGNN ANNNNNNNNN N //
Primer: IRES-R2 (Pr0247) Region: IRES2, mCherry 3' Sequence file: RDB19219_B1Grc.seq
>D08145B8_B1Gr_2_IRES-R2_C01_07_ABI24.ab1     1 GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGANAGGGG    61 CGCTAGCCTA CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT   121 CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC TTGATGTTGA   181 CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCAG   241 CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC   301 CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA   361 CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT   421 CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC   481 GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT   541 GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA CAGGATGTCC CAGGCGAAGG   601 GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG TAGGGGCGGC   661 CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC ATGTGCACCT   721 TGAAGCGCAT GAACTCCTTG ATGATGNCCA TGTTATCCTC CTCGCCCTTG CTCACCATGG   781 TGGCGAATTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTNAT ATAGGCCTCC   841 CACCGTACAC GCCTACCTCG ACATACGTTC TCTATCNCNG ATAGGGNNTA AACTCGACAT   901 ACNTTCTCTA TCACNGATAG GGATAAACTC NACNTACNNT CNCTATCANN NNNAGGGNNN 961 NANNNCNNNN TACNNTNNTC NNNNNNNNNN //
Primer: mRFP-M_R (Pr0674) Region: mCherry 5', tight TRE pro Sequence file: RDB19219_B1Grd.seq
>D08145B8_B1Gr_2_mRFP-M_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGCCTNNGNN CCGTNNATGN ACTGNNGGGN NAGGNNGTCC    61 CAGGCGAAGG GCAGGNGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG GGTGCCCTCG   121 TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC GGAGCCCTCC   181 ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC CTCGCCCTTG   241 CTCACCATGG TGGCGAATTC TCCAGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT   301 ATAGGCCTCC CACCGTACAC GCCTACCTCG ACATACGTTC TCTATCACTG ATAGGGAGTA   361 AACTCGACAT ACGTTCTCTA TCACTGATAG GGATAAACTC GACATACGTT CTCTATCACT   421 GATAGGGAGT AAACTCGACA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG   481 TTCTCTATCA CTGATAGGGA GTAAACTCGA CATCGTTCTC TATCACTGAT AGGGAGTAAA   541 CTCGACATAC GTTCTCTATC ACTGATAGGG AGTAAACTCG AGTGAAGACG AAAGGGCCTC   601 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA NGGTTTCTTA GACGTCAGGT   661 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA   721 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG   781 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTNG CGGCATTTTG   841 CCTTCCTGNT TTTGCTCACC CANAAACGCT GGNGAAAGTN AAAGANGCTG AAGATCAGTT   901 GGGNGNNNGA NNGGGTTACA TCNAACTNGN TCTCNNNNGC NNNNAGANNC NNNANANTTT 961 TCNNCCNNNN NNNNNTTNNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl