Mammalian expression vector of human synaptojanin fused with AcGFP at N-terminus.
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| Clone info. | Mammalian expression vector of human synaptojanin fused with AcGFP at N-terminus. |
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| Comment | Expression was confirmed by the depositor with microscopy. |
| Vector backbone | pcDNA-DEST40 (plasmid) |
| Size of vector backbone | 7.1 kb |
| Selectable markers | Ampicillin |
| Growth conditions | LB+Ampicillin, 37℃ |
| Gene/insert name | human SYNJ1 cDNA |
| Depositor|Developer | Abe, Mitsuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment In order to receive this biological resource, please use the Exclusive MTA [Link]. Please visit Information of Request for Distribution.[link] |
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| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested (Abe, M. et al., Cell Rep. 37: 109935 (2021)). |
| Remarks | Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC. |
[open/close]| 必要書類 | 提供依頼書 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Abe, M. et al., Cell Rep. 37: 109935 (2021))。 |
| 備考 | Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB19217 | pDEST/AcGFP-synaptojanin | DNA solution |
Materials & Methods section:
| The pDEST/AcGFP-synaptojanin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19217). |
Reference section:
| Abe, M., Makino, A., Murate, M., Hullin-Matsuda, F., Yanagawa, M., Sako, Y., Kobayashi, T., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [PubMed] [Article] [RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
| original | Abe, M., PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane. Cell Rep. 37 (6): 109935 (2021). PMID 34758297. [PubMed] [Article] [RRC of NBRP] |
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