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pMAL/MBP-lysenin

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.

Catalog number RDB13958
Resource name pMAL/MBP-lysenin
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.
Comment Expression was confirmed by the depositor.
Vector backbone pMAL-c2X (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
Eisenia foetida lysenin

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., Biochemistry 43 (30): 9766-9773, 2004.)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., Biochemistry 43 (30): 9766-9773, 2004.)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 タンパク質毒素遺伝子です (シマミミズ lysenin)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13958 pMAL/MBP-lysenin DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Kiyokawa, E., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13958_A6Btp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: MBP_937-993
            Region: MBP 3',Factor Xa site,insert 5'
            Sequence file: RDB13958_A6Bta.seq check
            >D03144A1_A6Bt_1_MBP_937_993_A04_01.ab1
                1 CCCAATTCGC TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC
               61 AGACTGTCGA TGAAGCCCTG AAAGACGCGC AGACTAATTC GAGCTCGAAC AACAACAACA
              121 ATAACAATAA CAACAACCTC GGGATCGAGG GAAGGATTTC AGAATTCGGA TCCATGTCGG
              181 CTAAAGCAGC AGAGGGATAT GAACAGATAG AAGTAGATGT GGTGGCAGTA TGGAAAGAAG
              241 GCTATGTATA CGAAAATCGC GGAAGCACCA GTGTGGATCA GAAGATCACA ATAACAAAAG
              301 GCATGAAAAA TGTGAATTCA GAAACAAGGA CAGTGACTGC TACGCATAGT ATTGGTTCTA
              361 CTATTAGCAC TGGAGATGCA TTCGAAATTG GAAGCGTGGA GGTTAGCTAC AGCCATTCAC
              421 ATGAAGAATC CCAAGTTAGC ATGACGGAAA CTGAAGTTTA TGAATCAAAG GTGATCGAAC
              481 ACACTATAAC GATTCCACCT ACTTCAAAAT TCACAAGATG GCAACTGAAT GCTGACGTTG
              541 GTGGAGCGGA TATTGAATAC ATGTATTTGA TTGATGAAGT CACACCCATA GGAGGGACTC
              601 AGAGTATTCC ACAGGTCATC ACAAGTCGGG CTAAAATTAT AGTTGGCCGA CAGATAATCC
              661 TTGGAAAAAC AGAAATTCGA ATTAAGCATG CAGAAAGGAA GGAGTACATG ACAGTCGTTT
              721 CAAGAAAAAG TTGGCCAGCT GCAACTCTTG GACATAGCAA ACTTTTCAAG TTTGTGCTCT
              781 ATGAAGATTG GGGGGGATTT CGAATTAAAA CGCTGAACAC CATGTATTCG GGCTATGAGT
              841 ATGCCTATTC CTCTGATCAA GGAGGAATCT ACTTTGATCA GGGTACTGAT AATCCGAAAC
              901 AGCGCTGGGC AATCAATAAG TCATTGCCTC TTCGTCATGG TGACGTAGTC ACCTTCATGA
              961 ATAAGTACTT CACTCGCAGT GGGCTGTGCT ACGATGATGG ACCGGCAACA AACGTGTACT
             1021 GTCTGGGACA ACGTGAAGAC AGTGGATTTG GAAGTGGTTG GTACCCCTGA ATGGGTGTCA
             1081 TGATGGTCAG CAGCATCACA TTCGCAATTG GCCTTCTGGT CATAATATTT CCACCTTTAA
            1141 GCCACATGGA ATTGAG
            //
            Primer: pTrcHis-R
            Region: insert 3'
            Sequence file: RDB13958_A6Btb.seq check
            >D03144A1_A6Bt_1_pTrcHis_R_B12_06.ab1
                1 CGGGGGGGGC GGGCTGATCT TATCTCATCC GCCAAAACAG CCAAGCTGCC ATTCGCCATT
               61 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT
              121 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC
              181 ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTCCGCTT TAGTTGCACC TCATCATTGT
              241 GCTAAAGGTG AAATATATGA CAGAAGCAAA TGCGAAATGG TGATGCTTGC TGACCAATCA
              301 TGACACCCCA TTTCAGGGGT TAACCAACCA CTTCCAAAAT CCACTTGTCT TCACGTTTGT
              361 CCAGACAGTA CACGTTTGTT GCCGGTCCAT CATCGTAGCA CAGCCCACTG CGAGTGAAGT
              421 ACTTATTCAT GAAGGTGACT ACGTCACCAT GACGAAGAGG CAATGACTTA TTGATTGCCC
              481 AGCGCTGTTT CGGATTATCA GTACCCTGAT CAAAGTAGAT TCCTCCTTGA TCAGAGGAAT
              541 AGGCATACTC ATAGCCCGAA TACATGGTGT TCAGCGTTTT AATTTCGAAA TCCCCCCCAA
              601 TCTTCATAGA GCACAAACTT GAAAAGTTTG CTATGTCCAA GAGTTGCACC TGGGCACTTT
              661 TTCTTGAAAC GACTGTCATG TACTCCTTCC TTTCTGCTGC TTTAATCCGA ATTTCCGTTT
              721 TTCCAAGGAT TATCTGTCGG CAATTATAAT TTTAGCCGAA CTGGGGATGA CCTGTGGAAT
            781 AGTCTAAGTT CCTC
            //
            Primer: lacI_C_F
            Region: lacI,lac pro,lac O,MBP 5'
            Sequence file: RDB13958_A6Btc.seq check
            >D03144A1_A6Bt_1_lacI_C_F_A12_03.ab1
                1 AACAATAGCA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA
               61 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC
              121 ATTAGGCACA ATTCTCATGT TTGACAGCTT ATCATCGACT GCACGGTGCA CCAATGCTTC
              181 TGGCGTCAGG CAGCCATCGG AAGCTGTGGT ATGGCTGTGC AGGTCGTAAA TCACTGCATA
              241 ATTCGTGTCG CTCAAGGCGC ACTCCCGTTC TGGATAATGT TTTTTGCGCC GACATCATAA
              301 CGGTTCTGGC AAATATTCTG AAATGAGCTG TTGACAATTA ATCATCGGCT CGTATAATGT
              361 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CCAGTCCGTT TAGGTGTTTT
              421 CACGAGCACT TCACCAACAA GGACCATAGC ATATGAAAAT CGAAGAAGGT AAACTGGTAA
              481 TCTGGATTAA CGGCGATAAA GGCTATAACG GTCTCGCTGA AGTCGGTAAG AAATTCGAGA
              541 AAGATACCGG AATTAAAGTC ACCGTTGAGC ATCCGGATAA ACTGGAAGAG AAATTCCCAC
              601 AGGTTGCGGC AACTGGCGAT GGCCCTGACA TTATCTTCTG GGCACACGAC CGCTTTGGTG
              661 GCTACGCTCA ATCTGGCCTG TTGGCTGAAA TCACCCCGGA CAAAGCGTTC CAGGACAAGC
              721 TGTATCCGTT TACCTGGGAT GCCGTACGTT ACAACGGCAA GCTGATTGCT TACCCGATCG
              781 CTGTTGAAGC GTTATCGCTG ATTTATAACA AAGATCTGCT GCCGAACCCG CCAAAAACCT
              841 GGGAAGAGAT CCCGGCGCTG GATAAAGAAC TGAAAGCGAA AGGTAAGAGC GCGCTGATGT
              901 TCAACCTGCA AGAACCGTAC TTCACCTGGC CGCTGATTGC TGCTGACGGG GGTTATGCGT
              961 TCAAGTATGA AAACGGCAAG TACGGACATT AAGGACGTGG GCGTGGGATA ACGCTGGCGC
             1021 GAAAGGCGGG TCTGACCTTC CTGGTGACCT GAATTAAAAA CAACCACATT GATGCAGACA
             1081 CCGATAACTC CATTCGGCAG AAGCTTGCCA TTTATAAAGG CGAACCAGAC CGGAATGTGG
            1141 AC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kiyokawa, E., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631.

            2022.05.17

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