Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMAL/MBP-lysenin

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., Biochemistry 43 (30): 9766-9773, 2004.)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., Biochemistry 43 (30): 9766-9773, 2004.)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	タンパク質毒素遺伝子です (シマミミズ lysenin)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13958_A6Btp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MBP_937-993 Region: MBP 3',Factor Xa site,insert 5' Sequence file: RDB13958_A6Bta.seq
>D03144A1_A6Bt_1_MBP_937_993_A04_01.ab1     1 CCCAATTCGC TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC    61 AGACTGTCGA TGAAGCCCTG AAAGACGCGC AGACTAATTC GAGCTCGAAC AACAACAACA   121 ATAACAATAA CAACAACCTC GGGATCGAGG GAAGGATTTC AGAATTCGGA TCCATGTCGG   181 CTAAAGCAGC AGAGGGATAT GAACAGATAG AAGTAGATGT GGTGGCAGTA TGGAAAGAAG   241 GCTATGTATA CGAAAATCGC GGAAGCACCA GTGTGGATCA GAAGATCACA ATAACAAAAG   301 GCATGAAAAA TGTGAATTCA GAAACAAGGA CAGTGACTGC TACGCATAGT ATTGGTTCTA   361 CTATTAGCAC TGGAGATGCA TTCGAAATTG GAAGCGTGGA GGTTAGCTAC AGCCATTCAC   421 ATGAAGAATC CCAAGTTAGC ATGACGGAAA CTGAAGTTTA TGAATCAAAG GTGATCGAAC   481 ACACTATAAC GATTCCACCT ACTTCAAAAT TCACAAGATG GCAACTGAAT GCTGACGTTG   541 GTGGAGCGGA TATTGAATAC ATGTATTTGA TTGATGAAGT CACACCCATA GGAGGGACTC   601 AGAGTATTCC ACAGGTCATC ACAAGTCGGG CTAAAATTAT AGTTGGCCGA CAGATAATCC   661 TTGGAAAAAC AGAAATTCGA ATTAAGCATG CAGAAAGGAA GGAGTACATG ACAGTCGTTT   721 CAAGAAAAAG TTGGCCAGCT GCAACTCTTG GACATAGCAA ACTTTTCAAG TTTGTGCTCT   781 ATGAAGATTG GGGGGGATTT CGAATTAAAA CGCTGAACAC CATGTATTCG GGCTATGAGT   841 ATGCCTATTC CTCTGATCAA GGAGGAATCT ACTTTGATCA GGGTACTGAT AATCCGAAAC   901 AGCGCTGGGC AATCAATAAG TCATTGCCTC TTCGTCATGG TGACGTAGTC ACCTTCATGA   961 ATAAGTACTT CACTCGCAGT GGGCTGTGCT ACGATGATGG ACCGGCAACA AACGTGTACT  1021 GTCTGGGACA ACGTGAAGAC AGTGGATTTG GAAGTGGTTG GTACCCCTGA ATGGGTGTCA  1081 TGATGGTCAG CAGCATCACA TTCGCAATTG GCCTTCTGGT CATAATATTT CCACCTTTAA 1141 GCCACATGGA ATTGAG //
Primer: pTrcHis-R Region: insert 3' Sequence file: RDB13958_A6Btb.seq
>D03144A1_A6Bt_1_pTrcHis_R_B12_06.ab1     1 CGGGGGGGGC GGGCTGATCT TATCTCATCC GCCAAAACAG CCAAGCTGCC ATTCGCCATT    61 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT   121 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC   181 ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTCCGCTT TAGTTGCACC TCATCATTGT   241 GCTAAAGGTG AAATATATGA CAGAAGCAAA TGCGAAATGG TGATGCTTGC TGACCAATCA   301 TGACACCCCA TTTCAGGGGT TAACCAACCA CTTCCAAAAT CCACTTGTCT TCACGTTTGT   361 CCAGACAGTA CACGTTTGTT GCCGGTCCAT CATCGTAGCA CAGCCCACTG CGAGTGAAGT   421 ACTTATTCAT GAAGGTGACT ACGTCACCAT GACGAAGAGG CAATGACTTA TTGATTGCCC   481 AGCGCTGTTT CGGATTATCA GTACCCTGAT CAAAGTAGAT TCCTCCTTGA TCAGAGGAAT   541 AGGCATACTC ATAGCCCGAA TACATGGTGT TCAGCGTTTT AATTTCGAAA TCCCCCCCAA   601 TCTTCATAGA GCACAAACTT GAAAAGTTTG CTATGTCCAA GAGTTGCACC TGGGCACTTT   661 TTCTTGAAAC GACTGTCATG TACTCCTTCC TTTCTGCTGC TTTAATCCGA ATTTCCGTTT   721 TTCCAAGGAT TATCTGTCGG CAATTATAAT TTTAGCCGAA CTGGGGATGA CCTGTGGAAT 781 AGTCTAAGTT CCTC //
Primer: lacI_C_F Region: lacI,lac pro,lac O,MBP 5' Sequence file: RDB13958_A6Btc.seq
>D03144A1_A6Bt_1_lacI_C_F_A12_03.ab1     1 AACAATAGCA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA    61 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC   121 ATTAGGCACA ATTCTCATGT TTGACAGCTT ATCATCGACT GCACGGTGCA CCAATGCTTC   181 TGGCGTCAGG CAGCCATCGG AAGCTGTGGT ATGGCTGTGC AGGTCGTAAA TCACTGCATA   241 ATTCGTGTCG CTCAAGGCGC ACTCCCGTTC TGGATAATGT TTTTTGCGCC GACATCATAA   301 CGGTTCTGGC AAATATTCTG AAATGAGCTG TTGACAATTA ATCATCGGCT CGTATAATGT   361 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CCAGTCCGTT TAGGTGTTTT   421 CACGAGCACT TCACCAACAA GGACCATAGC ATATGAAAAT CGAAGAAGGT AAACTGGTAA   481 TCTGGATTAA CGGCGATAAA GGCTATAACG GTCTCGCTGA AGTCGGTAAG AAATTCGAGA   541 AAGATACCGG AATTAAAGTC ACCGTTGAGC ATCCGGATAA ACTGGAAGAG AAATTCCCAC   601 AGGTTGCGGC AACTGGCGAT GGCCCTGACA TTATCTTCTG GGCACACGAC CGCTTTGGTG   661 GCTACGCTCA ATCTGGCCTG TTGGCTGAAA TCACCCCGGA CAAAGCGTTC CAGGACAAGC   721 TGTATCCGTT TACCTGGGAT GCCGTACGTT ACAACGGCAA GCTGATTGCT TACCCGATCG   781 CTGTTGAAGC GTTATCGCTG ATTTATAACA AAGATCTGCT GCCGAACCCG CCAAAAACCT   841 GGGAAGAGAT CCCGGCGCTG GATAAAGAAC TGAAAGCGAA AGGTAAGAGC GCGCTGATGT   901 TCAACCTGCA AGAACCGTAC TTCACCTGGC CGCTGATTGC TGCTGACGGG GGTTATGCGT   961 TCAAGTATGA AAACGGCAAG TACGGACATT AAGGACGTGG GCGTGGGATA ACGCTGGCGC  1021 GAAAGGCGGG TCTGACCTTC CTGGTGACCT GAATTAAAAA CAACCACATT GATGCAGACA  1081 CCGATAACTC CATTCGGCAG AAGCTTGCCA TTTATAAAGG CGAACCAGAC CGGAATGTGG 1141 AC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl