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pCMV-Halo-TubbyC (#RDB20671)

Expression vector of the Tubby C-terminal domain, which interacts with PI(4,5)P2. HaloTag(R) 7 was fused to the N terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Abe, M., Elife 13: e101652 (2024). PMID 39513999. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pHalo–TubbyC
Clone info. Expression vector of the Tubby C-terminal domain, which interacts with PI(4,5)P2. HaloTag(R) 7 was fused to the N terminus.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human TUB cDNA; Rhodococcus rhodochrous dehalogenase (HaloTag7).
Depositor|Developer Abe, Mitsuhiro |

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Abe, M. et al., eLife, Nov 8, 2024 https://doi.org/10.7554/eLife.101652) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the RIKEN CPR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees tobe bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abe, M. et al., eLife, Nov 8, 2024 https://doi.org/10.7554/eLife.101652)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、理研CPRの許可を得ること。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB20671 pCMV-Halo-TubbyC DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMV-Halo-TubbyC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20671).

Reference section:

Abe, M., Yanagawa, M., Hiroshima, M., Kobayashi, T., Sako, Y., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. [PubMed] [Article] [RRC of NBRP]