Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28/EryA-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.

Catalog number RDB13964
Resource name pET28/EryA-EGFP-His6
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.
Comment Expression was confirmed by the depositor.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Km^r
Gene/insert name Pleurotus eryngii EryA cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
Pleurotus eryngii EryA
    Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13964 pET28/EryA-EGFP-His6 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13964_A6Btp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pBR 322 BamHI R
            Sequence file: RDB13964_A6Bta.seq check
            >D03144A7_A6Bt_1_pBR 322 BamHI R_H04_22.ab1
                1 CTCATCAAGC ATTAAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT
               61 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC GGTACCATGG CATACGCTCA
              121 ATGGGTCATC ATCCTCATCC ACAACGTCGG GCAACAGAAC GTCAAGATCA AAAACCTCAA
              181 TGCTTCCTGG GGAAAGCTTT ATGCTGACGG CGACAAAGAC ACCGAAGTCC CAGCAAGCAA
              241 GTACGAAGGA ATGGTTATAG CGCCTGACGA CCAAGTCCAG ATCAACGCCT GTGGCAGAGA
              301 AGATGCTGCC GAGGGCACGA CAGGAACTTT TGACCTCGTC GACCCCAACG ACAGTGATAA
              361 GCAGGTCCGC CATTTTGCCT GGGACTGCCC CTGGGGAACC AAGGCCAACA GCTGGGTCGT
              421 TGGCGGGTCC AACTCCAAGT GGATGATCGA GTACACCGGT CAGAACTTGG ATAGTGGTGC
              481 CCTTGGCACG ATCACTGTTA ACACCTTGAG GATCGGGAAC GAATTCGGAG GAGGAGGAGG
              541 AGGATCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
              601 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
              661 CACCTATGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCCGTGCCCC
              721 TGGCCCCACC CTCGTGACCA CCCCTGACCT ACGGCGTGCA GTGCTTCACC CGGCTACCCC
              781 CGACCCACAT GGAAGCAGCA CGACTTCCTT CAAGTCCGCC ATGCCCGAAA GGCTACCTCC
            841 AGGAGCGCAC CCATCCTTCA TCAAAGGACG AACGGCAACT AC
            //
            Primer: T7Terminator
            Sequence file: RDB13964_A6Btb.seq check
            >D03144A7_A6Bt_1_T7Terminator_G04_19.ab1
                1 CAACGGCTTC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGAAG
               61 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG
              121 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA
              181 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC
              241 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC
              301 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT
              361 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC
              421 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA
              481 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
              541 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG
              601 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC
              661 ATAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC
              721 GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT
              781 GGATCCTCCT CCTCCTCCTC CGAATTCGTT CCCGATCCTC AAGGTGTTAA CAGTGATCGT
              841 GCCAAGGGCA CCACTATCCA AGTTCTGACC GGTGTACTCG ATCATCCACT TGGAGTTGGA
              901 CCCGCCAACG ACCCCAGCTG TTGGGCCTTG GTTCCCCAAG GGCAGTCCCA GGCAAAATTG
              961 GCCGGGACCT TGCCTTATCA CTGTCGTTGG GGTTCGACGA GGTCAAAGTT TCCTGGTCGT
             1021 GCCCTCGGCA GCATCATCTC TGCCACAGCG TTGAATCTGG GACTTGGTCG TCAGGGCGCT
             1081 AATAAACCAA TCCCTCGTAC TTGGCCTGGC TGGACTCCGG GTGTCTTTGT ATCGCCCGGA
            1141 TCA
            //
            Primer: pGL3_RV4
            Sequence file: RDB13964_A6Btc.seq check
            >D03144A7_A6Bt_1_pGL3_RV4_H09_24.ab1
                1 CGAACTCTGG GTTGAGGCTC TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG
               61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
              121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
              181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
              241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
              301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
              361 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
              421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
              481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
              541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
              601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC
              661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA
              721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT
              781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG
              841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA
              901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC
              961 TGGATGTGGC AACGCCCAAT CAGCAACGAC TGTTTGCCCG GCCCAGGTTG GTGGTGGCCA
             1021 CGCGGATGGG AAATGGTAAT TCAGGCCTCG TCATTCGCCG CATCACCTTT TCGCGATTTC
            1081 GCAGAAACGG TGGCCTTGGA CCTGGCTTCA CCTAAGGCCG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl