Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/EryA-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13964_A6Btp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR 322 BamHI R () Sequence file: RDB13964_A6Bta.seq
>D03144A7_A6Bt_1_pBR 322 BamHI R_H04_22.ab1     1 CTCATCAAGC ATTAAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT    61 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC GGTACCATGG CATACGCTCA   121 ATGGGTCATC ATCCTCATCC ACAACGTCGG GCAACAGAAC GTCAAGATCA AAAACCTCAA   181 TGCTTCCTGG GGAAAGCTTT ATGCTGACGG CGACAAAGAC ACCGAAGTCC CAGCAAGCAA   241 GTACGAAGGA ATGGTTATAG CGCCTGACGA CCAAGTCCAG ATCAACGCCT GTGGCAGAGA   301 AGATGCTGCC GAGGGCACGA CAGGAACTTT TGACCTCGTC GACCCCAACG ACAGTGATAA   361 GCAGGTCCGC CATTTTGCCT GGGACTGCCC CTGGGGAACC AAGGCCAACA GCTGGGTCGT   421 TGGCGGGTCC AACTCCAAGT GGATGATCGA GTACACCGGT CAGAACTTGG ATAGTGGTGC   481 CCTTGGCACG ATCACTGTTA ACACCTTGAG GATCGGGAAC GAATTCGGAG GAGGAGGAGG   541 AGGATCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA   601 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC   661 CACCTATGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCCGTGCCCC   721 TGGCCCCACC CTCGTGACCA CCCCTGACCT ACGGCGTGCA GTGCTTCACC CGGCTACCCC   781 CGACCCACAT GGAAGCAGCA CGACTTCCTT CAAGTCCGCC ATGCCCGAAA GGCTACCTCC 841 AGGAGCGCAC CCATCCTTCA TCAAAGGACG AACGGCAACT AC //
Primer: T7Terminator () Sequence file: RDB13964_A6Btb.seq
>D03144A7_A6Bt_1_T7Terminator_G04_19.ab1     1 CAACGGCTTC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGAAG    61 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG   121 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA   181 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC   241 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC   301 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT   361 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC   421 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA   481 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG   541 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG   601 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC   661 ATAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC   721 GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT   781 GGATCCTCCT CCTCCTCCTC CGAATTCGTT CCCGATCCTC AAGGTGTTAA CAGTGATCGT   841 GCCAAGGGCA CCACTATCCA AGTTCTGACC GGTGTACTCG ATCATCCACT TGGAGTTGGA   901 CCCGCCAACG ACCCCAGCTG TTGGGCCTTG GTTCCCCAAG GGCAGTCCCA GGCAAAATTG   961 GCCGGGACCT TGCCTTATCA CTGTCGTTGG GGTTCGACGA GGTCAAAGTT TCCTGGTCGT  1021 GCCCTCGGCA GCATCATCTC TGCCACAGCG TTGAATCTGG GACTTGGTCG TCAGGGCGCT  1081 AATAAACCAA TCCCTCGTAC TTGGCCTGGC TGGACTCCGG GTGTCTTTGT ATCGCCCGGA 1141 TCA //
Primer: pGL3_RV4 () Sequence file: RDB13964_A6Btc.seq
>D03144A7_A6Bt_1_pGL3_RV4_H09_24.ab1     1 CGAACTCTGG GTTGAGGCTC TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG    61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG   121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA   181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC   241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT   301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA   361 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG   421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA   481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA   541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC   601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC   661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA   721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT   781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG   841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA   901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC   961 TGGATGTGGC AACGCCCAAT CAGCAACGAC TGTTTGCCCG GCCCAGGTTG GTGGTGGCCA  1021 CGCGGATGGG AAATGGTAAT TCAGGCCTCG TCATTCGCCG CATCACCTTT TCGCGATTTC 1081 GCAGAAACGG TGGCCTTGGA CCTGGCTTCA CCTAAGGCCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl