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pET28/EryA-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.

Catalog number RDB13964
Resource name pET28/EryA-EGFP-His6
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.
Expression was confirmed by the depositor.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Km^r
Gene/insert name Pleurotus eryngii EryA cDNA Aequorea victoria GFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)
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Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
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RDB13964 pET28/EryA-EGFP-His6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13964_A6Btp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR 322 BamHI R RDB13964_A6Bta.seq
>D03144A7_A6Bt_1_pBR 322 BamHI R_H04_22.ab1
    1 CTCATCAAGC ATTAAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT
   61 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC GGTACCATGG CATACGCTCA
  121 ATGGGTCATC ATCCTCATCC ACAACGTCGG GCAACAGAAC GTCAAGATCA AAAACCTCAA
  181 TGCTTCCTGG GGAAAGCTTT ATGCTGACGG CGACAAAGAC ACCGAAGTCC CAGCAAGCAA
  241 GTACGAAGGA ATGGTTATAG CGCCTGACGA CCAAGTCCAG ATCAACGCCT GTGGCAGAGA
  301 AGATGCTGCC GAGGGCACGA CAGGAACTTT TGACCTCGTC GACCCCAACG ACAGTGATAA
  361 GCAGGTCCGC CATTTTGCCT GGGACTGCCC CTGGGGAACC AAGGCCAACA GCTGGGTCGT
  421 TGGCGGGTCC AACTCCAAGT GGATGATCGA GTACACCGGT CAGAACTTGG ATAGTGGTGC
  481 CCTTGGCACG ATCACTGTTA ACACCTTGAG GATCGGGAAC GAATTCGGAG GAGGAGGAGG
  541 AGGATCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
  601 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
  661 CACCTATGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCCGTGCCCC
  721 TGGCCCCACC CTCGTGACCA CCCCTGACCT ACGGCGTGCA GTGCTTCACC CGGCTACCCC
  781 CGACCCACAT GGAAGCAGCA CGACTTCCTT CAAGTCCGCC ATGCCCGAAA GGCTACCTCC
841 AGGAGCGCAC CCATCCTTCA TCAAAGGACG AACGGCAACT AC
//
Primer: T7Terminator RDB13964_A6Btb.seq
>D03144A7_A6Bt_1_T7Terminator_G04_19.ab1
    1 CAACGGCTTC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGAAG
   61 CTTCTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG
  121 GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA
  181 GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC
  241 GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC
  301 GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT
  361 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC
  421 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA
  481 GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG
  541 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG
  601 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC
  661 ATAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC
  721 GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT
  781 GGATCCTCCT CCTCCTCCTC CGAATTCGTT CCCGATCCTC AAGGTGTTAA CAGTGATCGT
  841 GCCAAGGGCA CCACTATCCA AGTTCTGACC GGTGTACTCG ATCATCCACT TGGAGTTGGA
  901 CCCGCCAACG ACCCCAGCTG TTGGGCCTTG GTTCCCCAAG GGCAGTCCCA GGCAAAATTG
  961 GCCGGGACCT TGCCTTATCA CTGTCGTTGG GGTTCGACGA GGTCAAAGTT TCCTGGTCGT
 1021 GCCCTCGGCA GCATCATCTC TGCCACAGCG TTGAATCTGG GACTTGGTCG TCAGGGCGCT
 1081 AATAAACCAA TCCCTCGTAC TTGGCCTGGC TGGACTCCGG GTGTCTTTGT ATCGCCCGGA
1141 TCA
//
Primer: pGL3_RV4 RDB13964_A6Btc.seq
>D03144A7_A6Bt_1_pGL3_RV4_H09_24.ab1
    1 CGAACTCTGG GTTGAGGCTC TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG
   61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
  121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
  181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
  241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
  301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
  361 CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
  421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
  481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
  541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
  601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC
  661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA
  721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT
  781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG
  841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA
  901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC
  961 TGGATGTGGC AACGCCCAAT CAGCAACGAC TGTTTGCCCG GCCCAGGTTG GTGGTGGCCA
 1021 CGCGGATGGG AAATGGTAAT TCAGGCCTCG TCATTCGCCG CATCACCTTT TCGCGATTTC
1081 GCAGAAACGG TGGCCTTGGA CCTGGCTTCA CCTAAGGCCG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

2019.06.21

GNP_filter3_RDBDEP_html_190430.pl