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pET28/Oly-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.

Catalog number RDB13963
Resource name pET28/Oly-EGFP-His6
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.
Comment Expression was confirmed by the depositor.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Km^r
Gene/insert name Pleurotus ostreatus Oly cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
Pleurotus ostreatus Oly
    Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13963 pET28/Oly-EGFP-His6 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13963_A6Btp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pBR322_BamHI_R
            Sequence file: RDB13963_A6Bta.seq check
            >D03144A6_A6Bt_1_pBR 322 BamHI R_F04_16.ab1
                1 CTCTCAAAAA ATTTAATACG ACTCACTATA GGGGATTGTG AGCGGATAAC AATTCCCCTC
               61 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCG GTACCATGGC TTATGCTCAA
              121 TGGGTCATCA TCCTGATCCA CAATGTCGGT CAACAAAATG TCAAAATCAA AAACCTGAAC
              181 GCCAGTTGGG GCAAGCTGTA TGCCGATGGC GACAAAGATA CCGAAGTGCC GGCAAGCAAG
              241 TACGAAGGCA TGGTCATTGC TCCGGGTGAC CAGGTGCAAA TCAACGCGTG CGGTCGTGAA
              301 GATGCGGCCG AAGGCACCAC CGGTACGTTT GACCTGGTTG ATCCGAATGA CAGTGATAAA
              361 CAGGTCCGCC ATATTTCCTG GGATTGTCCG TGGGGCACCA AAGCGAACAG CTGGGTGGTT
              421 GGCGGTAGCA ATTCTAAGTG GATGATCGAA TATACGGGTC AAAACCTGGA CTCTGGCGCA
              481 CTGGGCACGA TTACCGTCAA CACGCTGAAG ATTGGCAACG GATCCATGGT GAGCAAGGGC
              541 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
              601 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTATGGCAA AGCTGACCCT
              661 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCCTG GCCCACCCTC GTGACCCACC
              721 CTGACCTACG GCGTGCAGTG CCTTCAGCCG CTACCCCGAA CCACATGAAA GCAGCACGAC
              781 TTTCTTCAAG TCCCGCCCAT GCCCGAAAGC TACGTCCAGG ACCCGCACCA ATCTTCTTCA
            841 CGGGACGACA GGCAACCTAC AAGACCCCGC GCCAAGATGG A
            //
            Primer: T7 terminator
            Sequence file: RDB13963_A6Btb.seq check
            >D03144A6_A6Bt_1_T7Terminator_E04_13.ab1
                1 GACTGCATCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGAAGC
               61 TTCTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG
              121 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG
              181 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
              241 GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG
              301 TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG
              361 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
              421 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
              481 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
              541 TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG
              601 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCA
              661 TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
              721 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
              781 GATCCGTTGC CAATCTTCAG CGTGTTGACG GTAATCGTGC CCAGTGCGCC AGAGTCCAGG
              841 TTTTGACCCG TATATTCGAT CATCCACTTA GAATTGCTAC CGCCCAACCA CCCAGCTGTT
              901 CGCTTTGGTG CCCACGGACA ATCCCAGGAA ATATGGCGGA CCTGTTTATC ACTGTCATTC
              961 GGATCAACCA GGTCAAACGT ACCGGGTGGT GCCCTTCGCG CATCCTCCAC GACGCACGCC
             1021 GTTTGATTGC AACTGGGTCA CCCGAGCAAT GACAATGGCC ATCGTACTTG GCTTGGCCAG
            1081 GCACTCAGTA ATCTTGGTCG CAATCGGCAA TACAGGCCTT GCACCCGAC
            //
            Primer: pGL3_RV4
            Sequence file: RDB13963_A6Btc.seq check
            >D03144A6_A6Bt_1_pGL3_RV4_H12_24.ab1
                1 GAGACGCATG GCGTTGACGC TCTCAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA
               61 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
              121 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC
              181 AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG
              241 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT
              301 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT
              361 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC
              421 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC
              481 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA
              541 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA
              601 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG
              661 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG
              721 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
              781 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC
              841 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC
              901 AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA
              961 CTGGATGTGG GCAACGCCCA ATCAGCAACG ACTGTTGCCC GCCCAGTTGT TGTGCCACGC
             1021 GGATTGGGAA TGTAATTCAG CTCCGGCCAT CGGCGGCCTT CACTTTTTCC CGCGTTTTCG
            1081 CAGAACGTTG GCCTTGGCCT GGAATCCACC ACGGCCGGGG GA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl