Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/Oly-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13963_A6Btp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R Sequence file: RDB13963_A6Bta.seq
>D03144A6_A6Bt_1_pBR 322 BamHI R_F04_16.ab1     1 CTCTCAAAAA ATTTAATACG ACTCACTATA GGGGATTGTG AGCGGATAAC AATTCCCCTC    61 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCG GTACCATGGC TTATGCTCAA   121 TGGGTCATCA TCCTGATCCA CAATGTCGGT CAACAAAATG TCAAAATCAA AAACCTGAAC   181 GCCAGTTGGG GCAAGCTGTA TGCCGATGGC GACAAAGATA CCGAAGTGCC GGCAAGCAAG   241 TACGAAGGCA TGGTCATTGC TCCGGGTGAC CAGGTGCAAA TCAACGCGTG CGGTCGTGAA   301 GATGCGGCCG AAGGCACCAC CGGTACGTTT GACCTGGTTG ATCCGAATGA CAGTGATAAA   361 CAGGTCCGCC ATATTTCCTG GGATTGTCCG TGGGGCACCA AAGCGAACAG CTGGGTGGTT   421 GGCGGTAGCA ATTCTAAGTG GATGATCGAA TATACGGGTC AAAACCTGGA CTCTGGCGCA   481 CTGGGCACGA TTACCGTCAA CACGCTGAAG ATTGGCAACG GATCCATGGT GAGCAAGGGC   541 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   601 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTATGGCAA AGCTGACCCT   661 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCCTG GCCCACCCTC GTGACCCACC   721 CTGACCTACG GCGTGCAGTG CCTTCAGCCG CTACCCCGAA CCACATGAAA GCAGCACGAC   781 TTTCTTCAAG TCCCGCCCAT GCCCGAAAGC TACGTCCAGG ACCCGCACCA ATCTTCTTCA 841 CGGGACGACA GGCAACCTAC AAGACCCCGC GCCAAGATGG A //
Primer: T7 terminator Sequence file: RDB13963_A6Btb.seq
>D03144A6_A6Bt_1_T7Terminator_E04_13.ab1     1 GACTGCATCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGAAGC    61 TTCTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG   121 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG   181 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG   241 GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG   301 TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG   361 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC   421 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG   481 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC   541 TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG   601 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCA   661 TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG   721 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG   781 GATCCGTTGC CAATCTTCAG CGTGTTGACG GTAATCGTGC CCAGTGCGCC AGAGTCCAGG   841 TTTTGACCCG TATATTCGAT CATCCACTTA GAATTGCTAC CGCCCAACCA CCCAGCTGTT   901 CGCTTTGGTG CCCACGGACA ATCCCAGGAA ATATGGCGGA CCTGTTTATC ACTGTCATTC   961 GGATCAACCA GGTCAAACGT ACCGGGTGGT GCCCTTCGCG CATCCTCCAC GACGCACGCC  1021 GTTTGATTGC AACTGGGTCA CCCGAGCAAT GACAATGGCC ATCGTACTTG GCTTGGCCAG 1081 GCACTCAGTA ATCTTGGTCG CAATCGGCAA TACAGGCCTT GCACCCGAC //
Primer: pGL3_RV4 Sequence file: RDB13963_A6Btc.seq
>D03144A6_A6Bt_1_pGL3_RV4_H12_24.ab1     1 GAGACGCATG GCGTTGACGC TCTCAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA    61 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT   121 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC   181 AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG   241 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT   301 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT   361 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC   421 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC   481 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA   541 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA   601 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG   661 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG   721 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG   781 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC   841 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC   901 AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA   961 CTGGATGTGG GCAACGCCCA ATCAGCAACG ACTGTTGCCC GCCCAGTTGT TGTGCCACGC  1021 GGATTGGGAA TGTAATTCAG CTCCGGCCAT CGGCGGCCTT CACTTTTTCC CGCGTTTTCG 1081 CAGAACGTTG GCCTTGGCCT GGAATCCACC ACGGCCGGGG GA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl