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pET28/Oly-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.

Catalog number RDB13963
Resource name pET28/Oly-EGFP-His6
Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.
Expression was confirmed by the depositor.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Km^r
Gene/insert name Pleurotus ostreatus Oly cDNA Aequorea victoria GFP cDNA
Depositor Kobayashi, Toshihide | Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., FASEB J. 29 (9): 3920-3934, 2015.)
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13963 pET28/Oly-EGFP-His6 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Sphingolipid signaling pathway (English text)
Featured content Organelle marker (English text)

References

original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13963_A6Btp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R RDB13963_A6Bta.seq
>D03144A6_A6Bt_1_pBR 322 BamHI R_F04_16.ab1
    1 CTCTCAAAAA ATTTAATACG ACTCACTATA GGGGATTGTG AGCGGATAAC AATTCCCCTC
   61 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCG GTACCATGGC TTATGCTCAA
  121 TGGGTCATCA TCCTGATCCA CAATGTCGGT CAACAAAATG TCAAAATCAA AAACCTGAAC
  181 GCCAGTTGGG GCAAGCTGTA TGCCGATGGC GACAAAGATA CCGAAGTGCC GGCAAGCAAG
  241 TACGAAGGCA TGGTCATTGC TCCGGGTGAC CAGGTGCAAA TCAACGCGTG CGGTCGTGAA
  301 GATGCGGCCG AAGGCACCAC CGGTACGTTT GACCTGGTTG ATCCGAATGA CAGTGATAAA
  361 CAGGTCCGCC ATATTTCCTG GGATTGTCCG TGGGGCACCA AAGCGAACAG CTGGGTGGTT
  421 GGCGGTAGCA ATTCTAAGTG GATGATCGAA TATACGGGTC AAAACCTGGA CTCTGGCGCA
  481 CTGGGCACGA TTACCGTCAA CACGCTGAAG ATTGGCAACG GATCCATGGT GAGCAAGGGC
  541 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  601 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTATGGCAA AGCTGACCCT
  661 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCCTG GCCCACCCTC GTGACCCACC
  721 CTGACCTACG GCGTGCAGTG CCTTCAGCCG CTACCCCGAA CCACATGAAA GCAGCACGAC
  781 TTTCTTCAAG TCCCGCCCAT GCCCGAAAGC TACGTCCAGG ACCCGCACCA ATCTTCTTCA
841 CGGGACGACA GGCAACCTAC AAGACCCCGC GCCAAGATGG A
//
Primer: T7 terminator RDB13963_A6Btb.seq
>D03144A6_A6Bt_1_T7Terminator_E04_13.ab1
    1 GACTGCATCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGAAGC
   61 TTCTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG
  121 ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG
  181 TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG
  241 GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG
  301 TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG
  361 TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC
  421 AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG
  481 AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC
  541 TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG
  601 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCA
  661 TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  721 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG
  781 GATCCGTTGC CAATCTTCAG CGTGTTGACG GTAATCGTGC CCAGTGCGCC AGAGTCCAGG
  841 TTTTGACCCG TATATTCGAT CATCCACTTA GAATTGCTAC CGCCCAACCA CCCAGCTGTT
  901 CGCTTTGGTG CCCACGGACA ATCCCAGGAA ATATGGCGGA CCTGTTTATC ACTGTCATTC
  961 GGATCAACCA GGTCAAACGT ACCGGGTGGT GCCCTTCGCG CATCCTCCAC GACGCACGCC
 1021 GTTTGATTGC AACTGGGTCA CCCGAGCAAT GACAATGGCC ATCGTACTTG GCTTGGCCAG
1081 GCACTCAGTA ATCTTGGTCG CAATCGGCAA TACAGGCCTT GCACCCGAC
//
Primer: pGL3_RV4 RDB13963_A6Btc.seq
>D03144A6_A6Bt_1_pGL3_RV4_H12_24.ab1
    1 GAGACGCATG GCGTTGACGC TCTCAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA
   61 GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
  121 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC
  181 AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG
  241 CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT
  301 TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT
  361 ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC
  421 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC
  481 ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA
  541 ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA
  601 CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG
  661 CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG
  721 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
  781 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC
  841 GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC
  901 AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA
  961 CTGGATGTGG GCAACGCCCA ATCAGCAACG ACTGTTGCCC GCCCAGTTGT TGTGCCACGC
 1021 GGATTGGGAA TGTAATTCAG CTCCGGCCAT CGGCGGCCTT CACTTTTTCC CGCGTTTTCG
1081 CAGAACGTTG GCCTTGGCCT GGAATCCACC ACGGCCGGGG GA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Bhat, H.B., Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates. FASEB J. 29 (9): 3920-3934 (2015). PMID 26060215.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl