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Noboru Mizushima's web page in English / in Japanese
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| Catalog # | Name of clone | Running title | Publication |
| RDB20245 | pMXs-puro GFP-p62 dC | Retroviral vector for stable expression of rat p62 dC with N-terminal EGFP. | Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP] |
| RDB20244 | pMXs-IP-EGFP-LC3 | Retroviral vector for stable expression of rat LC3 with N-terminal EGFP. | Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP] |
| RDB20243 | p3xFLAG-CMV10-mApg16L | Transient expression vector of mouse Atg16l1 with N-terminal 3×FLAG. | Mizushima, N., Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J. Cell Sci. 116 (Pt 9): 1679-1688 (2023). PMID 12665549. (original). [link to RRC of NBRP] |
| RDB20084 | pMRX-IPU-muGFP-TAX1BP1 | Retroviral vector for stable expression of human TAX1BP1 with codon-optimised ultra-stable GFP (muGFP) tag. | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB20083 | pMRX-IPU-muGFP-NDP52 | Retroviral vector for stable expression of human NDP52 with codon-optimised ultra-stable GFP (muGFP) tag | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB20082 | pMRX-IPU-muGFP-NBR1 | Retroviral vector for stable expression of human NBR1 with codon-optimised ultra-stable GFP (muGFP) tag | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB20081 | pMRX-IPU-muGFP-p62 | Retroviral vector for stable expression of human p62 with codon-optimised ultra-stable GFP (muGFP) tag | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB20080 | pMRX-IPU-muGFP-Ubiquitin | Retroviral vector for stable expression of human ubiquitin with codon-optimised ultra-stable GFP (muGFP) tag | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB20079 | pMRX-IPU-muGFP-OPTN | Retroviral vector for stable expression of human OPTN with codon-optimised ultra-stable GFP (muGFP) tag | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB20078 | pMRX-IPU-muGFP-OPTN delta UBAN | Retroviral vector for stable expression of human OPTN (without ubiquitin binding domain UBAN) with codon-optimised ultra-stable GFP (muGFP) tag | Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. (original). [link to RRC of NBRP] |
| RDB19968 | pMRX-IP-HaloTag7-LC3 | Retroviral vector for stable expression of HaloTag7-LC3 to measure autophagic flux. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19967 | pMRX-No-HaloTag7-LC3 | Retroviral vector for stable expression of HaloTag7-LC3 to measure autophagic flux. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19966 | pMRX-No-HaloTag7-mGFP-LC3 | Retroviral vector for stable expression of HaloTag7-mGFP-LC3 to measure autophagic flux. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19965 | pMRX-No-HaloTag7-mGFP-LC3-mRFP | Retroviral vector for stable expression of HaloTag7-mGFP-LC3-mRFP to measure autophagic flux. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19964 | pMRX-IB-HaloTag7-mGFP | Retroviral vector for stable expression of HaloTag7-mGFP to measure autophagic flux. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19963 | pMRX-IB-HaloTag7-mGFP-KDEL | Retroviral vector for stable expression of HaloTag7-mGFP-KDEL to measure ER-phagy activity. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19962 | pMRX-IB-pSu9-HaloTag7-mGFP | Retroviral vector for stable expression of pSu9-HaloTag7-mGFP to measure mitophagy activity. | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP] |
| RDB19804 | pcDNA3-GFP-LC3-RFP-LC3 deltaG | A autophagic flux probe, expression vector of GFP-rat LC3-RFP-rat LC3 deletion G. | Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. (original). [link to RRC of NBRP] |
| RDB19803 | pCI-neo-HA-hUVRAG | Expression vector of human UVRAG with N-terminal HA. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19802 | pCI-neo-HA-hAtg13 | Expression vector of human Atg13 with N-terminal HA. | Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. (original). [link to RRC of NBRP] |
| RDB19800 | p3xFLAG-CMV10-hTti1 | Transient expression vector of human Tti1 with N-terminal 3×FLAG. | Kaizuka, T., Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J. Biol. Chem. 285 (26): 20109-20116 (2010). PMID 20427287. (original). [link to RRC of NBRP] |
| RDB19799 | pCI-neo-hApg5(K130R)-HA | Expression vector of human Apg5 mutant (K130R) with C-terminal HA. | Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. (original). [link to RRC of NBRP] |
| RDB19797 | pCI-neo-HA-hAtg14 | Expression vector of human Atg14 with N-terminal HA. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19796 | pMXs-puro-GFP-p62 D337, 338, 339A | Retroviral vector for stable expression of mouse p62 with N-terminal GFP. | Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP] |
| RDB19795 | p3xFLAG-CMV10-hTel2 | Transient expression vector of human Tel2 with N-terminal 3×FLAG. | Kaizuka, T., Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J. Biol. Chem. 285 (26): 20109-20116 (2010). PMID 20427287. (original). [link to RRC of NBRP] |
| RDB19794 | pMXs-puro-GFP-p62 K7A, D69A | Retroviral vector for stable expression of mouse p62 mutant (K7A/D69A) with N-terminal GFP. | Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP] |
| RDB19793 | pMRXIP-GFP-Stx17TM | Retroviral vector for stable expression of human Stx17TM with N-terminal GFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19792 | pMRXIP-GFP-Stx18 | Retroviral vector for stable expression of human Stx18 with N-terminal GFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19791 | pCI-neo-HA-hApg12 | Expression vector of human Apg12 with N-terminal HA. | Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. (original). [link to RRC of NBRP] |
| RDB19790 | pMXs-IP-GFP-Atg14 | Retroviral vector for stable expression of human Atg14 with N-terminal EGFP. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19789 | pEGFP-C1-mApg5(K130R) | Transient expression vector of mouse Apg5 mutant (K130R) with N-terminal GFP. | Mizushima, N., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. (original). [link to RRC of NBRP] |
| RDB19788 | pMXs-puro HA-WIPI-1 | Retroviral vector for stable expression of human WIPI-1 with N-terminal HA. | Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP] |
| RDB19787 | pMRXIP-VPS16-GFP | Retroviral vector for stable expression of human VPS16 with C-terminal EGFP. | Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. (original). [link to RRC of NBRP] |
| RDB19786 | pMXs-IP-GFP-Omp25 | Retroviral vector for stable expression of rat Omp25 with N-terminal EGFP. | Yoshii, S.R., Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J. Biol. Chem. 286 (22): 19630-19640 (2011). PMID 21454557. (original). [link to RRC of NBRP] |
| RDB19785 | pEGFP-C1-hAtg14 | Transient expression vector of human ATG14 with N-terminal EGFP. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19784 | pEGFP-C1-mApg5 | Transient expression vector of mouse Apg5 with N-terminal EGFP. | Mizushima, N., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. (original). [link to RRC of NBRP] |
| RDB19783 | pMXsIP-GFP-VPS33A | Retroviral vector for stable expression of human VPS33A with N-terminal GFP. | Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. (original). [link to RRC of NBRP] |
| RDB19782 | pMRXIP-GFP-hATG2A | Retroviral vector for stable expression of human ATG2A with N-terminal GFP. | Tamura, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. (original). [link to RRC of NBRP] |
| RDB19781 | pMRX-INU-FLAG-RTN3L | Retroviral vector for stable expression of human RTN3L with N-terminal FLAG. | Chino, H., Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol. Cell 74 (5): 909-921.e6 (2019). PMID 31006538. (original). [link to RRC of NBRP] |
| RDB19780 | pMXs-IP-EGFP-ULK1(K46N) | Retroviral vector for stable expression of mouse ULK1(K46N) with N-terminal EGFP. | Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP] |
| RDB19779 | pMRXIP GFP-Stx7 | Expression vector of human Stx7 with N-terminal GFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19778 | p3xFLAG-CMV10-VAMP7 | Transient expression vector of mouse VAMP7 with N-terminal FLAG. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19776 | p3xFLAG-CMV10-hUVRAG | Transient expression vector of human UVRAG with N-terminal 3×FLAG. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19775 | pCI-neo-hApg5-HA | Expression vector of human Apg5 with C-terminal HA. | Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. (original). [link to RRC of NBRP] |
| RDB19773 | p3xFLAG-CMV10-hAtg101 | Transient expression vector of human Atg101 with N-terminal 3×FLAG. | Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. (original). [link to RRC of NBRP] |
| RDB19772 | p3xFLAG-CMV14-mULK1 | Transient expression vector of mouse ULK1 with N-terminal 3×FLAG. | Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP] |
| RDB19771 | p3xFLAG-CMV10-hAtg13 | Transient expression vector of human Atg13 with N-terminal 3×FLAG. | Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP] |
| RDB19770 | pEGFP-C1-hAtg13 | Transient expression vector of human Atg13 with N-terminal EGFP. | Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP] |
| RDB19769 | pME18s-HA-hFIP200 | Expression vector of human FIP200 with N-terminal HA. | Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP] |
| RDB19768 | pMXs-IP spGFP-ERGIC53 | Retroviral vector for stable expression of human ERGIC-53 with N-terminal GFP. | Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP] |
| RDB19767 | pCW57-CMV-ssRFP-GFP-KDEL | Lentiviral vector for inducible expression of ER-phagy marker ssRFP-GFP-KDEL. | Chino, H., Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol. Cell 74 (5): 909-921.e6 (2019). PMID 31006538. (original). [link to RRC of NBRP] |
| RDB19765 | p3xFLAG-CMV10-SNAP29 | Transient expression vector of human SNAP29 with N-terminal FLAG. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19764 | pMXs-IP-HA-Parkin | Retroviral vector for stable expression of human Parkin with N-terminal HA. | Yoshii, S.R., Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J. Biol. Chem. 286 (22): 19630-19640 (2011). PMID 21454557. (original). [link to RRC of NBRP] |
| RDB19762 | p3xFLAG-CMV10-hFIP200 | Transient expression vector of human FIP200 with N-terminal 3×FLAG. | Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP] |
| RDB19761 | p3xFLAG-CMV10-Stx17 | Transient expression vector of human STX17 with N-terminal 3×FLAG. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19640 | pMRX-IP-GFP-VAMP8 | Retroviral vector for stable expression of human VAMP8 with N-terminal EGFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19639 | pMRX-IP-GFP-VAMP7 | Retroviral vector for stable expression of mouse VAMP7 with N-terminal EGFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19638 | pMRX-IP-GFP-SNAP29 | Retroviral vector for stable expression of human SNAP29 with N-terminal EGFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19637 | pMXs-puro-GFP-DFCP1 | Retroviral vector for stable expression of mouse DFCP1 with N-terminal EGFP. | Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP] |
| RDB19636 | pMRX-IP-GFP-STX17 | Retroviral vector for stable expression of human STX17/syntaxin 17 with N-terminal EGFP. | Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP] |
| RDB19635 | pMRX-IP-SECFP-hATG16L1 | Retroviral vector for stable expression of human ATG16L1 with N-terminal SECFP. | Koyama-Honda, I., Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9 (10): 1491-1499 (2013). PMID 23884233. (original). [link to RRC of NBRP] |
| RDB19634 | pMXs-IP-EGFP-mAtg5 | Retroviral vector for stable expression of mouse Atg5 with C-terminal EGFP. | Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP] |
| RDB19633 | pMXs-puro-VPS34-GFP | Retroviral vector for stable expression of human VPS34 with C-terminal EGFP. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19632 | pEGFP-C1-hUVRAG | Transient expression vector of human UVRAG with N-terminal EGFP. | Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP] |
| RDB19631 | pEGFP-C1-hATG2A | Transient expression vector of human ATG2A with N-terminal EGFP. | Velikkakath, A.K., Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol. Biol. Cell 23 (5): 896-909 (2012). PMID 22219374. (original). [link to RRC of NBRP] |
| RDB19630 | pMXs-IP-GFP-WIPI1 | Retroviral vector for stable expression of human WIPI1 with N-terminal EGFP. | Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP] |
| RDB19629 | pMXs-IP-GFP-NBR1 | Retroviral vector for stable expression of human NBR1 with N-terminal EGFP. | Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP] |
| RDB19628 | pMXs-puro-GFP-p62 | Retroviral vector for stable expression of mouse p62/SQSTM1 with N-terminal EGFP. | Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP] |
| RDB19627 | pMXs-puro-RFP-ATG9A | Retroviral vector for stable expression of human ATG9A with N-terminal RFP. | Koyama-Honda, I., Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9 (10): 1491-1499 (2013). PMID 23884233. (original). [link to RRC of NBRP] |
| RDB19626 | pEGFP-C1-hATG101 | Transient expression vector of human ATG101 with N-terminal EGFP. | Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. (original). [link to RRC of NBRP] |
| RDB19625 | pMXs-IP-EGFP-hATG13 | Retroviral vector for stable expression of human ATG13 with N-terminal EGFP. | Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP] |
| RDB19624 | pMRX-IP-EGFP-mULK1 | Retroviral vector for stable expression of mouse ULK1 with N-terminal EGFP. | Itakura, E., Structures containing Atg9A and the ULK1 complex independently target depolarized mitochondria at initial stages of Parkin-mediated mitophagy. J. Cell Sci. 125 (Pt 6): 1488-1499 (2012). PMID 22275429. (original). [link to RRC of NBRP] |
| RDB19623 | pMXs-IP-EGFP-hFIP200 | Retroviral vector for stable expression of human FIP200 with N-terminal EGFP. | Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP] |
| RDB19621 | pMRX-IP-hTMEM41B-3xFLAG (WT) | Retroviral vector for stable expression of human TMEM41B (WT) with C-terminal 3xFLAG. | Okawa, F., Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1. J. Cell Sci. 134 (8): jcs255877 (2021). PMID 33771928. (original). [link to RRC of NBRP] |
| RDB14601 | pMRX-IP-GFP-LC3-RFP | Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP. | Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. (original). [link to RRC of NBRP] |
| RDB14600 | pMRX-IP-GFP-LC3-RFP-LC3 delta G | Fluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP. | Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. (original). [link to RRC of NBRP] |
♦ No Approval Forms Required for Fluorescent Protein Genes Developed by Dr. Atsushi Miyawaki
♦ RIKEN BRC will be closed from December 28, 2024 through January 5, 2025 due to New Year's holidays.
♦ A bicistronic cell cycle reporter, Fucci2a (Sep 17, 2024)
♦ Monomeric Fluorescent Protein Resource, mStayGold (Dec 18, 2023)
♦ Visualization of Organelles update (Dec 18, 2023)
♦ Development of two mouse strains conditionally expressing bright luciferases (Sep 08, 2023)
♦ Autophagy and Mitophagy Updates (Aug 16, 2023)
♦ High intensity forms of luciferase and luminescent proteins from various organisms (BRC RESOURCE NEWS) (Apr 28, 2023)
♦ Plasmid of Cas9 expression/mRNA production, evaluation of the genome edit efficiency, and Knock-in donors and tags
♦ Fucci cell cycle indicator, Calcium sensor and Fluorescent and Luminescent protein resources
♦ Revision of Distribution Fees for Bioresources in RIKEN BRC
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2025.03.28
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