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Noboru Mizushima's Resource

Noboru Mizushima's web page in English /  in Japanese.

Learn more about their research, in English  /  in Japanese (by Reseachmap[link])

Catalog #Name of cloneRunning titlePublication
RDB14600pMRX-IP-GFP-LC3-RFP-LC3 delta GFluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP. Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. (original). [link to RRC of NBRP]
RDB14601pMRX-IP-GFP-LC3-RFPFluorescent probe to evaluate autophagic flux by the rat LC3 fused with GFP and RFP. Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. (original). [link to RRC of NBRP]
RDB19621pMRX-IP-hTMEM41B-3xFLAG (WT)Retroviral vector for stable expression of human TMEM41B (WT) with C-terminal 3xFLAG.Okawa, F., Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1. J. Cell Sci. 134 (8): jcs255877 (2021). PMID 33771928. (original). [link to RRC of NBRP]
RDB19623pMXs-IP-EGFP-hFIP200Retroviral vector for stable expression of human FIP200 with N-terminal EGFP.Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP]
RDB19624pMRX-IP-EGFP-mULK1Retroviral vector for stable expression of mouse ULK1 with N-terminal EGFP.Itakura, E., Structures containing Atg9A and the ULK1 complex independently target depolarized mitochondria at initial stages of Parkin-mediated mitophagy. J. Cell Sci. 125 (Pt 6): 1488-1499 (2012). PMID 22275429. (original). [link to RRC of NBRP]
RDB19625pMXs-IP-EGFP-hATG13Retroviral vector for stable expression of human ATG13 with N-terminal EGFP.Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP]
RDB19626pEGFP-C1-hATG101Transient expression vector of human ATG101 with N-terminal EGFP.Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. (original). [link to RRC of NBRP]
RDB19627pMXs-puro-RFP-ATG9ARetroviral vector for stable expression of human ATG9A with N-terminal RFP.Koyama-Honda, I., Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9 (10): 1491-1499 (2013). PMID 23884233. (original). [link to RRC of NBRP]
RDB19628pMXs-puro-GFP-p62Retroviral vector for stable expression of mouse p62/SQSTM1 with N-terminal EGFP.Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP]
RDB19629pMXs-IP-GFP-NBR1Retroviral vector for stable expression of human NBR1 with N-terminal EGFP.Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP]
RDB19630pMXs-IP-GFP-WIPI1Retroviral vector for stable expression of human WIPI1 with N-terminal EGFP.Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP]
RDB19631pEGFP-C1-hATG2ATransient expression vector of human ATG2A with N-terminal EGFP.Velikkakath, A.K., Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol. Biol. Cell 23 (5): 896-909 (2012). PMID 22219374. (original). [link to RRC of NBRP]
RDB19632pEGFP-C1-hUVRAGTransient expression vector of human UVRAG with N-terminal EGFP.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19633pMXs-puro-VPS34-GFPRetroviral vector for stable expression of human VPS34 with C-terminal EGFP.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19634pMXs-IP-EGFP-mAtg5Retroviral vector for stable expression of mouse Atg5 with C-terminal EGFP.Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP]
RDB19635pMRX-IP-SECFP-hATG16L1Retroviral vector for stable expression of human ATG16L1 with N-terminal SECFP.Koyama-Honda, I., Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site. Autophagy 9 (10): 1491-1499 (2013). PMID 23884233. (original). [link to RRC of NBRP]
RDB19636pMRX-IP-GFP-STX17Retroviral vector for stable expression of human STX17/syntaxin 17 with N-terminal EGFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19637pMXs-puro-GFP-DFCP1Retroviral vector for stable expression of mouse DFCP1 with N-terminal EGFP.Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP]
RDB19638pMRX-IP-GFP-SNAP29Retroviral vector for stable expression of human SNAP29 with N-terminal EGFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19639pMRX-IP-GFP-VAMP7Retroviral vector for stable expression of mouse VAMP7 with N-terminal EGFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19640pMRX-IP-GFP-VAMP8Retroviral vector for stable expression of human VAMP8 with N-terminal EGFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19761p3xFLAG-CMV10-Stx17Transient expression vector of human STX17 with N-terminal 3×FLAG.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19762p3xFLAG-CMV10-hFIP200Transient expression vector of human FIP200 with N-terminal 3×FLAG.Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP]
RDB19764pMXs-IP-HA-ParkinRetroviral vector for stable expression of human Parkin with N-terminal HA.Yoshii, S.R., Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J. Biol. Chem. 286 (22): 19630-19640 (2011). PMID 21454557. (original). [link to RRC of NBRP]
RDB19765p3xFLAG-CMV10-SNAP29Transient expression vector of human SNAP29 with N-terminal FLAG.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19767pCW57-CMV-ssRFP-GFP-KDELLentiviral vector for inducible expression of ER-phagy marker ssRFP-GFP-KDEL.Chino, H., Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol. Cell 74 (5): 909-921.e6 (2019). PMID 31006538. (original). [link to RRC of NBRP]
RDB19768pMXs-IP spGFP-ERGIC53Retroviral vector for stable expression of human ERGIC-53 with N-terminal GFP.Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP]
RDB19769pME18s-HA-hFIP200Expression vector of human FIP200 with N-terminal HA.Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP]
RDB19770pEGFP-C1-hAtg13Transient expression vector of human Atg13 with N-terminal EGFP.Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP]
RDB19771p3xFLAG-CMV10-hAtg13Transient expression vector of human Atg13 with N-terminal 3×FLAG.Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP]
RDB19772p3xFLAG-CMV14-mULK1Transient expression vector of mouse ULK1 with N-terminal 3×FLAG.Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. (original). [link to RRC of NBRP]
RDB19773p3xFLAG-CMV10-hAtg101Transient expression vector of human Atg101 with N-terminal 3×FLAG.Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. (original). [link to RRC of NBRP]
RDB19775pCI-neo-hApg5-HAExpression vector of human Apg5 with C-terminal HA.Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. (original). [link to RRC of NBRP]
RDB19776p3xFLAG-CMV10-hUVRAGTransient expression vector of human UVRAG with N-terminal 3×FLAG.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19778p3xFLAG-CMV10-VAMP7Transient expression vector of mouse VAMP7 with N-terminal FLAG.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19779pMRXIP GFP-Stx7Expression vector of human Stx7 with N-terminal GFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19780pMXs-IP-EGFP-ULK1(K46N)Retroviral vector for stable expression of mouse ULK1(K46N) with N-terminal EGFP.Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP]
RDB19781pMRX-INU-FLAG-RTN3LRetroviral vector for stable expression of human RTN3L with N-terminal FLAG.Chino, H., Intrinsically Disordered Protein TEX264 Mediates ER-phagy. Mol. Cell 74 (5): 909-921.e6 (2019). PMID 31006538. (original). [link to RRC of NBRP]
RDB19782pMRXIP-GFP-hATG2ARetroviral vector for stable expression of human ATG2A with N-terminal GFP.Tamura, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. (original). [link to RRC of NBRP]
RDB19783pMXsIP-GFP-VPS33ARetroviral vector for stable expression of human VPS33A with N-terminal GFP.Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. (original). [link to RRC of NBRP]
RDB19784pEGFP-C1-mApg5Transient expression vector of mouse Apg5 with N-terminal EGFP.Mizushima, N., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. (original). [link to RRC of NBRP]
RDB19785pEGFP-C1-hAtg14Transient expression vector of human ATG14 with N-terminal EGFP.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19786pMXs-IP-GFP-Omp25Retroviral vector for stable expression of rat Omp25 with N-terminal EGFP.Yoshii, S.R., Parkin mediates proteasome-dependent protein degradation and rupture of the outer mitochondrial membrane. J. Biol. Chem. 286 (22): 19630-19640 (2011). PMID 21454557. (original). [link to RRC of NBRP]
RDB19787pMRXIP-VPS16-GFPRetroviral vector for stable expression of human VPS16 with C-terminal EGFP.Jiang, P., The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol. Biol. Cell 25 (8): 1327-1337 (2014). PMID 24554770. (original). [link to RRC of NBRP]
RDB19788pMXs-puro HA-WIPI-1Retroviral vector for stable expression of human WIPI-1 with N-terminal HA.Itakura, E., Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins. Autophagy 6 (6): 764-776 (2010). PMID 20639694. (original). [link to RRC of NBRP]
RDB19789pEGFP-C1-mApg5(K130R)Transient expression vector of mouse Apg5 mutant (K130R) with N-terminal GFP.Mizushima, N., Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. J. Cell Biol. 152 (4): 657-668 (2001). PMID 11266458. (original). [link to RRC of NBRP]
RDB19790pMXs-IP-GFP-Atg14Retroviral vector for stable expression of human Atg14 with N-terminal EGFP.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19791pCI-neo-HA-hApg12Expression vector of human Apg12 with N-terminal HA.Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. (original). [link to RRC of NBRP]
RDB19792pMRXIP-GFP-Stx18Retroviral vector for stable expression of human Stx18 with N-terminal GFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19793pMRXIP-GFP-Stx17TMRetroviral vector for stable expression of human Stx17TM with N-terminal GFP.Itakura, E., The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151 (6): 1256-1269 (2012). PMID 23217709. (original). [link to RRC of NBRP]
RDB19794pMXs-puro-GFP-p62 K7A, D69ARetroviral vector for stable expression of mouse p62 mutant (K7A/D69A) with N-terminal GFP.Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP]
RDB19795p3xFLAG-CMV10-hTel2Transient expression vector of human Tel2 with N-terminal 3×FLAG.Kaizuka, T., Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J. Biol. Chem. 285 (26): 20109-20116 (2010). PMID 20427287. (original). [link to RRC of NBRP]
RDB19796pMXs-puro-GFP-p62 D337, 338, 339ARetroviral vector for stable expression of mouse p62 with N-terminal GFP.Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP]
RDB19797pCI-neo-HA-hAtg14Expression vector of human Atg14 with N-terminal HA.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19799pCI-neo-hApg5(K130R)-HAExpression vector of human Apg5 mutant (K130R) with C-terminal HA.Mizushima, N., A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. J. Biol. Chem. 273 (51): 33889-33892 (1998). PMID 9852036. (original). [link to RRC of NBRP]
RDB19800p3xFLAG-CMV10-hTti1Transient expression vector of human Tti1 with N-terminal 3×FLAG.Kaizuka, T., Tti1 and Tel2 are critical factors in mammalian target of rapamycin complex assembly. J. Biol. Chem. 285 (26): 20109-20116 (2010). PMID 20427287. (original). [link to RRC of NBRP]
RDB19802pCI-neo-HA-hAtg13Expression vector of human Atg13 with N-terminal HA.Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. (original). [link to RRC of NBRP]
RDB19803pCI-neo-HA-hUVRAGExpression vector of human UVRAG with N-terminal HA.Itakura, E., Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 19 (12): 5360-5372 (2008). PMID 18843052. (original). [link to RRC of NBRP]
RDB19804pcDNA3-GFP-LC3-RFP-LC3 deltaGA autophagic flux probe, expression vector of GFP-rat LC3-RFP-rat LC3 deletion G.Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. (original). [link to RRC of NBRP]
RDB19962pMRX-IB-pSu9-HaloTag7-mGFPRetroviral vector for stable expression of pSu9-HaloTag7-mGFP to measure mitophagy activity.
Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB19963pMRX-IB-HaloTag7-mGFP-KDELRetroviral vector for stable expression of HaloTag7-mGFP-KDEL to measure ER-phagy activity.
Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB19964pMRX-IB-HaloTag7-mGFPRetroviral vector for stable expression of HaloTag7-mGFP to measure autophagic flux.
Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB19965pMRX-No-HaloTag7-mGFP-LC3-mRFPRetroviral vector for stable expression of HaloTag7-mGFP-LC3-mRFP to measure autophagic flux.
Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB19966pMRX-No-HaloTag7-mGFP-LC3Retroviral vector for stable expression of HaloTag7-mGFP-LC3 to measure autophagic flux.
Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB19967pMRX-No-HaloTag7-LC3Retroviral vector for stable expression of HaloTag7-LC3 to measure autophagic flux.Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB19968pMRX-IP-HaloTag7-LC3Retroviral vector for stable expression of HaloTag7-LC3 to measure autophagic flux.Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. (original). [link to RRC of NBRP]
RDB20243p3xFLAG-CMV10-mApg16LTransient expression vector of mouse Atg16l1 with N-terminal 3×FLAG.Mizushima, N., Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J. Cell Sci. 116 (Pt 9): 1679-1688 (2023). PMID 12665549. (original). [link to RRC of NBRP]
RDB20244pMXs-IP-EGFP-LC3Retroviral vector for stable expression of rat LC3 with N-terminal EGFP.Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. (original). [link to RRC of NBRP]
RDB20245pMXs-puro GFP-p62 dCRetroviral vector for stable expression of rat p62 dC with N-terminal EGFP.Itakura, E., p62 Targeting to the autophagosome formation site requires self-oligomerization but not LC3 binding. J. Cell Biol. 192 (1): 17-27 (2011). PMID 21220506. (original). [link to RRC of NBRP]

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2024.07.09

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