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pMRXIP-GFP-hATG2A

Retroviral vector for stable expression of human ATG2A with N-terminal GFP.

Catalog number RDB19782
Resource name pMRXIP-GFP-hATG2A
Clone info. Retroviral vector for stable expression of human ATG2A with N-terminal GFP.
Vector backbone pMRX-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human ATG2A cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19782hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19782_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tamura, N. et al. FEBS Lett. 591(23): 3819-3830, 2017)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] 
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tamura, N. et al. FEBS Lett. 591(23): 3819-3830, 2017)。
付加的提供条件:
pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19782 pMRXIP-GFP-hATG2A DNA solution


How to cite this biological resource

Materials & Methods section:

The pMRXIP-GFP-hATG2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19782).

Reference section:

Tamura, N., Nishimura, T., Sakamaki, Y., Koyama-Honda, I., Yamamoto, H., Mizushima, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Tamura, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. [link to RRC of NBRP]