Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pMRX-IPU-muGFP-p62 (#RDB20081)

Retroviral vector for stable expression of human p62 with codon-optimised ultra-stable GFP (muGFP) tag

Clone info. Retroviral vector for stable expression of human p62 with codon-optimised ultra-stable GFP (muGFP) tag.
See Methods section of Yang Z, EMBO J. 43 (22): 5613-5634 (2024). doi: 10.1038/s44318-024-00272-5.
Vector backbone pMRX-IPU-GFP (plasmid)
Growth conditions LB+Ampicilin, 30oC
Growth remarks NEB Stable or an equivalent host is recommended for amplifying this clone.
Gene/insert name human p62 cDNA.
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB20081hts01.seq checkAssembled from experimentally sequenced data.
Publication Yang, Z., EMBO J. 43 (22): 5613-5634 (2024). [link to RRC of NBRP]
Test sheet RDB20081_B3I8p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Zi Yang et al., EMBO J. 43: 5613-5634, 2024)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Zi Yang et al., EMBO J. 43: 5613-5634, 2024)。
付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20081 pMRX-IPU-muGFP-p62 DNA solution

How to cite this biological resource

Materials & Methods section:

The pMRX-IPU-muGFP-p62 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20081).

Reference section:

Yang, Z., Yoshii, S.R., Sakai, Y., Zhang, J., Chino, H., Knorr, R.L., Mizushima, N., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yang, Z., Autophagy adaptors mediate Parkin-dependent mitophagy by forming sheet-like liquid condensates. EMBO J. 43 (22): 5613-5634 (2024). PMID 39420095. [link to RRC of NBRP]