Retroviral vector for stable expression of HaloTag7-mGFP-KDEL to measure ER-phagy activity.
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022) Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested. The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
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Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] |
Remarks | Remember that you will be working with samples containing infectious virus. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)。 付加的提供条件: pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
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提供依頼 | 手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB19963 | pMRX-IB-HaloTag7-mGFP-KDEL | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pMRX-IB-HaloTag7-mGFP-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19963). |
Reference section:
Yim, W.W., Yamamoto, H., Mizushima, N., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP] |
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2023.06.05
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