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pMRX-No-HaloTag7-mGFP-LC3-mRFP (#RDB19965)

Retroviral vector for stable expression of HaloTag7-mGFP-LC3-mRFP to measure autophagic flux.

Clone info. Retroviral vector for stable expression of HaloTag7-mGFP-rat LC3-mRFP without a drug resistance marker to measure autophagic flux. See, Yim, W.WY. et al., eLife 11: e78923, 2022.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pMRX-No (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name Rhodococcus rhodochrous HaloTag7; Aequorea victoria GFP (mGFP); rat LC3 cDNA; Discosoma sp. RFP (mRFP).
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19965hts01.seq checkAssembled from experimentally sequenced data.
Publication Yim, W.W., Elife 11: e78923 (2022). [link to RRC of NBRP]
Test sheet RDB19965_B3Dsp1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)
Additional terms and conditions:
Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Remarks Remember that you will be working with samples containing infectious virus.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Willa Wen-You Yim et al. eLife. 11:e78923, 2022)。
MTAに書く付加的使用条件：
pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19965 pMRX-No-HaloTag7-mGFP-LC3-mRFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pMRX-No-HaloTag7-mGFP-LC3-mRFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19965).

Reference section:

Yim, W.W., Yamamoto, H., Mizushima, N., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yim, W.W., A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag. Elife 11: e78923 (2022). PMID 35938926. [link to RRC of NBRP]