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p3xFLAG-CMV10-hAtg101 (#RDB19773)

Transient expression vector of human Atg101 with N-terminal 3×FLAG.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hosokawa, N., Autophagy 5 (7): 973-979 (2009). PMID 19597335. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Transient expression vector of human Atg101 with N-terminal 3×FLAG.
Vector backbone p3xFLAG-CMV-10 (plasmid)
Size of vector backbone 6.3 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name human ATG101 cDNA; synthetic 3×FLAG-tag.
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hosokawa, N. et al. Autophagy, 5(7): 973-979, 2009)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hosokawa, N. et al. Autophagy, 5(7): 973-979, 2009)。

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RDB19773 p3xFLAG-CMV10-hAtg101 DNA solution

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV10-hAtg101 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19773).

Reference section:

Hosokawa, N., Sasaki, T., Iemura, S., Natsume, T., Hara, T., Mizushima, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hosokawa, N., Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy 5 (7): 973-979 (2009). PMID 19597335. [PubMed] [Article] [RRC of NBRP]