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pMXs-IP-EGFP-ULK1(K46N)

Retroviral vector for stable expression of mouse ULK1(K46N) with N-terminal EGFP.

Catalog number RDB19780
Resource name pMXs-IP-EGFP-ULK1(K46N)
Clone info. Retroviral vector for stable expression of mouse ULK1(K46N) with N-terminal EGFP.
Vector backbone pMXs-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name mouse Ulk1 cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |
 
Sequence (full) RDB19780hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19780_B2Jlp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hara, T. et al. J. Cell Biol. 181(3): 497-510, 2008)
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] 
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hara, T. et al. J. Cell Biol. 181(3): 497-510, 2008)。
付加的提供条件:
pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19780 pMXs-IP-EGFP-ULK1(K46N) DNA solution


How to cite this biological resource

Materials & Methods section:

The pMXs-IP-EGFP-ULK1(K46N) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19780).

Reference section:

Hara, T., Takamura, A., Kishi, C., Iemura, S., Natsume, T., Guan, J.L., Mizushima, N., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Hara, T., FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. J. Cell Biol. 181 (3): 497-510 (2008). PMID 18443221. [link to RRC of NBRP]